evaluation of rapid one system for identification of 379 strains in the family enterobacteriaceae and oxidase-negative, gram-negative nonfermenters.the ability of the rapid one system (innovative diagnostic systems, inc., norcross, ga.) to identify 364 strains in the family enterobacteriaceae and 15 oxidase-negative, gram-negative, nonfermentative rods was evaluated. kits were inoculated with no. 2 mcfarland standard suspensions, and reactions were interpreted after 4 h of incubation at 35 degrees c. overall, the method correctly identified (to the species level or to the genus level for salmonellas and non-shigella sonnei shigella species) ...19948027345
enterobacter hormaechei bloodstream infection at three neonatal intensive care units in brazil.enterobacter hormaechei was defined as a unique species in 1989. we describe six case patients of e. hormaechei bloodstream infection in three neonatal intensive care units in rio de janeiro, brazil. e. hormaechei identification was performed on the vitek system and confirmed by conventional testing. strain relatedness was evaluated by pulsed field gel electrophoresis. all children recovered completely. chart review for previous procedures revealed parenteral nutrition as the only common procedu ...200211840092
chromosomal ampc genes in enterobacter species other than enterobacter cloacae, and ancestral association of the act-1 plasmid-encoded cephalosporinase to enterobacter asburiae.the amplification and sequence of ampc genes in enterobacter asburiae, enterobacter cancerogenus, enterobacter dissolvens, enterobacter hormaechei and enterobacter intermedius bring the number of known cephalosporinase sequences from the genus enterobacter to seven. expression in escherichia coli of the ampc genes from e. asburiae, e. hormaechei and e. intermedius established the functional nature of these genes. ampc from e. asburiae shows 96.5% identity to bla(act-1) encoding a plasmid-borne c ...200212023082
growth control of small-colony variants by genetic regulation of the hemin uptake system.small-colony variants (scvs) are slow-growing variants of human bacterial pathogens. they are associated with chronic persistent infections, and their biochemical identification and antimicrobial treatment are impaired by altered metabolic properties. to contribute to the understanding of scv-mediated infections, we analyzed a clinical scv isolate derived from a chronic prosthetic hip infection. a sequence analysis of housekeeping genes identified an enterobacter hormaechei-like organism. the sc ...200415039350
detection and characterization of extended-spectrum beta-lactamases among bloodstream isolates of enterobacter spp. in hong kong, 2000-2002.a total of 139 consecutive and non-duplicate bloodstream isolates of enterobacter spp. collected from inpatients in hong kong during 2000-2002 were studied for production of extended-spectrum beta-lactamases (esbls).200515681579
genomic diversity within the enterobacter cloacae complex.isolates of the enterobacter cloacae complex have been increasingly isolated as nosocomial pathogens, but phenotypic identification of the e. cloacae complex is unreliable and irreproducible. identification of species based on currently available genotyping tools is already superior to phenotypic identification, but the taxonomy of isolates belonging to this complex is cumbersome.200818716657
identification of resistance and virulence factors in an epidemic enterobacter hormaechei outbreak strain.bacterial strains differ in their ability to cause hospital outbreaks. using comparative genomic hybridization, enterobacter cloacae complex isolates were studied to identify genetic markers specific for enterobacter cloacae complex outbreak strains. no outbreak-specific genes were found that were common in all investigated outbreak strains. therefore, the aim of our study was to identify specific genetic markers for an enterobacter hormaechei outbreak strain (ehos) that caused a nationwide outb ...200919372158
isolation and characterization of rhamnolipid-producing bacterial strains from a biodiesel facility.novel strains of rhamnolipid-producing bacteria were isolated from soils at a biodiesel facility on the basis of their ability to grow on glycerol as a sole carbon source. strains were identified as acinetobacter calcoaceticus, enterobacter asburiae, enterobacter hormaechei, pantoea stewartii, and pseudomonas aeruginosa. the strains of the former five species were found to produce rhamnolipids in quantities the same as, or similar to, coisolated strains of p. aeruginosa. measurements of surface ...200919473254
molecular cloning and expression of a novel trehalose synthase gene from enterobacter hormaechei.abstract:200919523196
emergence of an enterobacter hormaechei strain with reduced susceptibility to tigecycline under tigecycline therapy. 200919738023
[effect of cultivation parameters of antarctic strains enterobacter hormaechei and brevibacterium antarcticumon resistant to copper(ii) ions].enterobacter hormaechei and brevibacterium antarcticum strains isolated from ornithogenic soils of galindez island (west antarctica) were investigated for their resistance to cu2+ cations and for their capacity to cu2+ uptake from the environment. the studied strains are capable to grow in the concentration range of copper 100-1100 mg/l and to extract 11-75% of cu2+ from the environment depending on cultivation parameters and copper output concentration in the culture medium.200919938609
evolution in quantum leaps: multiple combinatorial transfers of hpi and other genetic modules in enterobacteriaceae.horizontal gene transfer is a key step in the evolution of enterobacteriaceae. by acquiring virulence determinants of foreign origin, commensals can evolve into pathogens. in enterobacteriaceae, horizontal transfer of these virulence determinants is largely dependent on transfer by plasmids, phages, genomic islands (gis) and genomic modules (gms). the high pathogenicity island (hpi) is a gi encoding virulence genes that can be transferred between different enterobacteriaceae. we investigated the ...201020084283
isolates of the enterobacter cloacae complex induce apoptosis of human intestinal epithelial cells.strains of the enterobacter cloacae complex are becoming increasingly important human pathogen. the aim of the study was to identify, by sequencing the hsp60 gene, the species of clinical isolates phenotypically identified as e. cloacae and to examine them for virulence-associated properties: the ability of adhesion, invasion to hep-2 cells and the induced apoptosis of infected epithelial cells. the majority of the strains were identified as enterobacter hormaechei with e. hormaechei subsp. stei ...201020451600
cultivation of enterobacter hormaechei from human atherosclerotic determine whether culturable bacterial strains are present in human atheromatous tissue and to investigate their properties using culture, quantitative pcr, metagenomic screening, genomic and biochemical methods.201020972353
comparison of microbial diversity of edible flowers and basil grown with organic versus conventional methods.the consumption and use of edible flowers as food is growing; however, no study has been conducted to evaluate their role in the cause of food-borne illness or in food safety. recent food-borne outbreaks traced to fresh herbs have raised concern about their processing and handling. basil, one of the most commonly used fresh herbs, has been identified as a source of food-borne illness. baseline assessments of microflora were performed, and the microbial diversity between growing methods (organic ...201021076485
molecular epidemiological survey of citrobacter freundii misidentified as cronobacter spp. (enterobacter sakazakii) and enterobacter hormaechei isolated from powdered infant milk formula.a total of 75 powdered infant milk formula (pif) samples collected from pharmacies and drugstores in western sicily, italy, and representative of 12 different brands were analyzed in this study to evaluate their microbiological quality. according to the u.s. food and drug administration protocol, 32 samples out of 75 were contaminated by enterobacteria. commercial biochemical api(r) 20e-system identification method indicated that six pif samples were presumptively contaminated by cronobacter spp ...201121204699
metabolic surgery profoundly influences gut microbial-host metabolic cross-talk.background and aims bariatric surgery is increasingly performed worldwide to treat morbid obesity and is also known as metabolic surgery to reflect its beneficial metabolic effects especially with respect to improvement in type 2 diabetes. understanding surgical weight loss mechanisms and metabolic modulation is required to enhance patient benefits and operative outcomes. methods the authors applied a parallel and statistically integrated bacterial profiling and metabonomic approach to character ...201121572120
outbreak of a novel enterobacter sp. carrying bla(ctx-m-15) in a neonatal unit of a tertiary care hospital in tanzania.enterobacter hormaechei and cronobacter sakazakii are amongst the most important causes of outbreaks of neonatal sepsis associated with powdered milk. in this study, we report for the first time an outbreak of a novel enterobacter sp. harbouring bla(ctx-m-15) in a neonatal unit in tanzania. seventeen gram-negative enteric isolates from neonatal blood cultures were studied. antibiotic susceptibility was assessed by disc diffusion testing, and the presence of the bla(ctx-m-15) gene was established ...201121752606
Experimental bariatric surgery in rats generates a cytotoxic chemical environment in the gut contents.Bariatric surgery, also known as metabolic surgery, is an effective treatment for morbid obesity, which also offers pronounced metabolic effects including the resolution of type 2 diabetes and a decrease in cardiovascular disease and long-term cancer risk. However, the mechanisms of surgical weight loss and the long-term consequences of bariatric surgery remain unclear. Bariatric surgery has been demonstrated to alter the composition of both the microbiome and the metabolic phenotype. We observe ...201121949514
a dual approach employing maldi-tof ms and real-time pcr for fast species identification within the enterobacter cloacae complex.a real-time pcr procedure targeting the gene of the molecular cochaperon dnaj (dnaj) was developed for specific detection of strains belonging to the enterobacter cloacae group. the inclusivity and exclusivity of the real-time pcr assay were assessed with seven reference strains of e. cloacae, 12 other enterobacter species and 41 non-enterobacter strains. inclusivity as well as exclusivity of the duplex real-time pcr was 100%. in contrast, resolution of matrix-assisted laser desorption ionizatio ...201122150997
a novel triculture system (cc3) for simultaneous enzyme production and hydrolysis of common grasses through submerged fermentation.the perennial grasses are considered as a rich source of lignocellulosic biomass, making it a second generation alternative energy source and can diminish the use of fossil fuels. in this work, four perennial grasses saccharum arundinaceum, panicum antidotale, thysanolaena latifolia, and neyraudia reynaudiana were selected to verify their potential as a substrate to produce hydrolytic enzymes and to evaluate them as second generation energy biomass. here, cellulase and hemi-cellulase producing t ...201627065995
cultivable endophytic bacteria from leaf bases of agave tequilana and their role as plant growth promoters.agave tequilana weber var. 'azul' is grown for the production of tequila, inulin and syrup. diverse bacteria inhabit plant tissues and play a crucial role for plant health and growth. in this study culturable endophytic bacteria were extracted from leaf bases of 100 healthy agave tequilana plants. in plant tissue bacteria occurred at mean population densities of 3 million cfu/g of fresh plant tissue. three hundred endophytic strains were isolated and 16s rdna sequences grouped the bacteria into ...201425763038
pantoea agglomerans in immunodeficient patients with different respiratory symptoms.the aim of this paper was to determine in 32 patients from 4 different mexican hospitals the frequency of opportunistic bacteria in the 2010 to 2011 time period. the patients were divided in 4 groups. group 1 included 21 hiv positive patients with acute respiratory syndrome. four hiv positive patients with tuberculosis symptoms were included in group 2; two patients with tuberculosis symptoms and one asymptomatic person formed group 3. reference group 4 included 4 patients from whom 4 strains of ...201222619600
microbial diversity and dynamics during the production of may bryndza cheese.diversity and dynamics of microbial cultures were studied during the production of may bryndza cheese, a traditional slovak cheese produced from unpasteurized ewes' milk. quantitative culture-based data were obtained for lactobacilli, lactococci, total mesophilic aerobic counts, coliforms, e. coli, staphylococci, coagulase-positive staphylococci, yeasts, fungi and geotrichum spp. in ewes' milk, curd produced from it and ripened for 0 - 10 days, and in bryndza cheese produced from the curd, in th ...201424291178
characterization of carbapenem-resistant gram-negative bacteria from tamil nadu.carbapenem resistance is disseminating worldwide among gram-negative bacteria. the aim of this study was to identify carbapenem-resistance level and to determine the mechanism of carbapenem resistance among clinical isolates from two centres in tamil nadu. in the present study, a total of 93 gram-negative isolates, which is found to be resistant to carbapenem by disk diffusion test in two centres, were included. all isolates are identified at species level by 16s rrna sequencing. minimal inhibit ...201626198414
characterization of an extended-spectrum beta-lactamase enterobacter hormaechei nosocomial outbreak, and other enterobacter hormaechei misidentified as cronobacter (enterobacter) sakazakii.enterobacter hormaechei is a gram-negative bacterium within the enterobacter cloacae complex, and has been shown to be of clinical significance by causing nosocomial infections, including sepsis. ent. hormaechei is spread via horizontal transfer and is often associated with extended-spectrum beta-lactamase production, which increases the challenges associated with treatment by limiting therapeutic options. this report considers 10 strains of ent. hormaechei (identified by 16s rdna sequencing) th ...200819047733
analysis of bacterial diversity during the fermentation of inyu, a high-temperature fermented soy sauce, using nested pcr-denaturing gradient gel electrophoresis and the plate count method.the diversity of bacteria associated with the fermentation of inyu, also known as black soy sauce, was studied through the nested pcr-denaturing gradient gel electrophoresis (dgge) of samples collected from the fermentation stages of the inyu production process. the dgge profiles targeted the bacterial 16s rdna and revealed the presence of citrobacter farmeri, enterobacter cloacae, enterobacter hormaechei, enterococcus faecium, klebsiella pneumoniae, pantoea agglomerans, salmonella enterica, ser ...201323200659
high incidence of plant growth-stimulating bacteria associated with the rhizosphere of wheat grown on salinated soil in uzbekistan.soil salinization is increasing steadily in many parts of the world and causes major problems for plant productivity. under these stress conditions, root-associated beneficial bacteria can help improve plant growth and nutrition. in this study, salt-tolerant bacteria from the rhizosphere of uzbek wheat with potentially beneficial traits were isolated and characterized. eight strains which initially positively affect the growth of wheat plants in vitro were investigated in detail. all eight strai ...200818211262
detection of extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae in vegetables, soil and water of the farm environment in samples of 18 different farms (49 of food-vegetables, 41 of soil and 19 of irrigation water) and 45 vegetable food samples of 13 markets were collected in tunisia. these samples were inoculated in macconkey agar plates supplemented with cefotaxime (2 μg/ml). esbl-producing enterobacteriaceae (esbl-eb) were detected in 10 of the 109 farm samples (vegetables, 8.2%; soil, 7.3%; water, 15.8%), and in 4 of 45 vegetables of markets (8.9%), recovering 15 esbl-eb. isolates and esbl gene ...201525791254
outbreak of enterobacter hormaechei septicaemia in newborns caused by contaminated parenteral nutrition in brazil. 200717428576
bacterial reduction of selenium in coal mine tailings pond sediment.sediment from a storage facility for coal tailings solids was assessed for its capacity to reduce selenium (se) by native bacterial community. one se(6+)-reducing bacterium enterobacter hormaechei (tar11) and four se(4+)-reducing bacteria, klebsiella pneumoniae (tar1), pseudomonas fluorescens (tar3), stenotrophomonas maltophilia (tar6), and enterobacter amnigenus (tar8) were isolated from the sediment. enterobacter hormaechei removed 96% of the added se(6+) (0.92 mg l(-1)) from the effluents whe ...200717412898
identification of plasmid-mediated extended-spectrum and ampc beta-lactamases in enterobacter spp. isolated from dogs.the genetic determinants involved in reduced susceptibility to third-generation cephalosporins and aztreonam were identified in ten canine enterobacter isolates associated with opportunistic infections in three veterinary hospitals in brisbane, australia. all isolates were evaluated by a combination of phenotypic (broth microdilution and disc susceptibility, modified disc diffusion and ief) and genotypic (pfge, plasmid analysis, southern blot hybridization, bacterial conjugation, pcr and sequenc ...200717314376
rapid identification of enterobacteriaceae by sequencing dna gyrase subunit b encoding gene.real-time polymerase chain reaction and sequencing were used to characterize a 506-bp-long dna fragment internal to the gyrb gene (gyrbint). the sequences obtained from 32 enterobacteriaceae-type strains and those available in the genbank nucleotide sequence database (n = 24) were used as a database to identify 240 clinical enterobacteria isolates. sequence analysis of the gyrbint fragment of 240 strains showed that gyrbint constitutes a discriminative target sequence to differentiate between en ...200616626902
enterobacter hormaechei subsp. oharae subsp. nov., e. hormaechei subsp. hormaechei comb. nov., and e. hormaechei subsp. steigerwaltii subsp. nov., three new subspecies of clinical importance.six species and six additional genovars are combined within the so-called enterobacter cloacae complex, with one of them being the species enterobacter hormaechei. in a recent population genetic study, two genetic clusters were found in close phylogenetic proximity to the genetic cluster of e. hormaechei. in order to prove the hypothesis that these three genetic clusters belong to the same species, we performed cross-hybridization experiments in microplates with dnas of representatives of each g ...200516000451
description of enterobacter ludwigii sp. nov., a novel enterobacter species of clinical relevance.a new species, enterobacter ludwigii, is presented on the basis of the characteristics of 16 strains, which were isolated from clinical specimens. these bacteria form a distinct genetic cluster in phylogenetic analyses of the population structure of the enterobacter cloacae complex. as determined by dna-dna cross-hybridization experiments in microplates, this genetic cluster can be delineated from the other species of the e. cloacae complex with deltatm values equal to or above 5 degrees c with ...200515900967
the influence of endoscopic procedures upon the contamination of helicobacter pylori cultures.among the various diagnostic methods for the detection of helicobacter pylori infection, histological examination and microbiological processing of gastric biopsy samples are assumed to be the gold standard techniques.200415543382
population genetics of the nomenspecies enterobacter cloacae.the genetic heterogeneity of the nomenspecies enterobacter cloacae is well known. enterobacter asburiae, enterobacter cancerogenus, enterobacter dissolvens, enterobacter hormaechei, enterobacter kobei, and enterobacter nimipressuralis are closely related to it and are subsumed in the so-called e. cloacae complex. dna-dna hybridization studies performed previously identified at least five dna-relatedness groups of this complex. in order to analyze the genetic structure and the phylogenetic relati ...200312957918
core flooding tests to investigate the effects of ift reduction and wettability alteration on oil recovery during meor process in an iranian oil reservoir.microbial enhanced oil recovery (meor) refers to the process of using bacterial activities for more oil recovery from oil reservoirs mainly by interfacial tension reduction and wettability alteration mechanisms. investigating the impact of these two mechanisms on enhanced oil recovery during meor process is the main objective of this work. different analytical methods such as oil spreading and surface activity measurements were utilized to screen the biosurfactant-producing bacteria isolated fro ...201323553033
natural antibiotic susceptibility of strains of the enterobacter cloacae complex.the natural susceptibility to 70 antibiotics of 104 strains of the enterobacter cloacae complex was examined using a microdilution procedure in isosensitest broth. one hundred and one clinical strains designated as 'e. cloacae' were identified as e. hormaechei (n=65), e. asburiae (n=20) and e. cloacae genospecies 1 or 2 (n=16). apart from fosfomycin susceptibility, there were only minor differences in natural antibiotic susceptibility patterns. all species were naturally sensitive or naturally s ...200111738341
an outbreak of enterobacter hormaechei infection and colonization in an intensive care nursery.enterobacter hormaechei was first identified as a unique species in 1989. between 29 november 1992 and 17 march 1993, an outbreak of e. hormaechei occurred among premature infants in the intensive care nursery (icn) at the hospital of the university of pennsylvania. the 10 infants whose cultures were positive for e. hormaechei (six were infected and four were colonized) had a lower median estimated gestational age and birth weight than did other icn infants; other risk factors for infection or c ...19979195091
a nosocomial outbreak due to enterobacter cloacae strains with the e. hormaechei genotype in patients treated with fluoroquinolones.during a 7-month period, we isolated 21 highly fluoroquinolone-resistant enterobacter cloaecae strains in units from two hospitals in marseille, france. random amplification of polymorphic dna showed clonal identity between isolates which, furthermore, presented the enterobacter hormaechei genotype on dna-dna hybridization. the emergence of this clone was observed only in patients treated with fluoroquinolones.19979157119
enterobacter hormaechei, a new species of the family enterobacteriaceae formerly known as enteric group 75.the name enterobacter hormaechei is proposed for a new species of the family enterobacteriaceae, formerly called enteric group 75, which consists of 23 strains, 22 of which were isolated from humans. dnas from 12 e. hormaechei strains tested were highly related to the type strain (atcc 49162) by dna hybridization, using the hydroxyapatite method (80 to 97% in 60 degrees c reactions; 80 to 90% in 75 degrees c reactions). the strains were most closely related (50 to 63%) to enterobacter cloacae, e ...19892778068
isolation and identification of phosphate solubilizing bacteria able to enhance the growth and aloin-a biosynthesis of aloe barbadensis miller.the effect of four phosphate solubilizing bacteria (psb) was studied on growth and aloin-a content of aloe barbadensis in soil containing tricalcium phosphate (tcp). psb were identified based on 16s rrna gene sequencing as pseudomonas synxantha, burkholderia gladioli, enterobacter hormaechei and serratia marcescens. these psb solubilized 25-340 μg ml(-1) of tcp into the liquid phase. the treatment of plants with individual psb or mixture of these increased soil available p, p uptake in plants an ...201222417676
first report of ndm-1-producing acinetobacter baumannii sequence type 25 in delhi metallo-β-lactamase 1 (ndm-1) was first identified in brazil in enterobacter hormaechei and providencia rettgeri in 2013. here, we describe the first case of ndm-1-producing acinetobacter baumannii sequence type 25 isolated from the urinary tract of a 71-year-old man who died of multiple complications, including a. baumannii infection. the ndm-1 gene was detected by quantitative pcr, and its sequence confirmed its presence in an ∼ 100-kb plasmid.201425288087
characterization by culture-dependent and culture-independent methods of the bacterial population of suckling-lamb packaged in different atmospheres.this study offers insight into the dynamics of bacterial populations in fresh cuts of suckling lamb under four different atmospheric conditions: air (a), and three modified atmosphere packaging (map) environments, 15%o2/30%co2/55%n2 (c, commercial), 70%o2/30%co2 (o), and 15%o2/85%co2 (h) for 18 days. microbial analyses by both conventional methods and pcr-dgge were performed. controversial and surprising results emerged from comparing both methods in relation to the genus pseudomonas. thus, conv ...201324010600
molecular characterization by using next-generation sequencing of plasmids containing blandm-7 in enterobacteriaceae from calgary, canada.enterobacteriaceae with blandm-7 are relatively uncommon and had previously been described in europe, india, the united states, and japan. this study describes the characteristics of enterobacteriaceae (klebsiella pneumoniae [n = 2], escherichia coli [n = 2], serratia marcescens [n = 1], and enterobacter hormaechei [n = 1] isolates) with blandm-7 obtained from 4 patients from calgary, canada, from 2013 to 2014. the 46,161-bp incx3 plasmids with blandm-7 are highly similar to other blandm-harbori ...201626643346
draft genome sequences of three ndm-1-producing enterobacteriaceae species isolated from brazil.the emergence of multidrug-resistant enterobacteriaceae strains producing carbapenemases, such as ndm-1, has become a major public health issue due to a high dissemination capacity and limited treatment options. here we describe the draft genome of three ndm-1-producing isolates: providencia rettgeri (ccbh11880), enterobacter hormaechei subsp. oharae (ccbh10892) and klebsiella pneumoniae (ccbh13327), isolated in brazil. besides blandm-1, resistance genes to aminoglycosides [aada1, aada2, aac(6') ...201526061151
clonal dissemination of oxa-370-producing klebsiella pneumoniae in rio de janeiro, brazil.enzymes of the oxa-48 family have become some of the most important beta-lactamases in the world. a new oxa-48 variant (oxa-370) was first described for an enterobacter hormaechei strain isolated in rio grande do sul (southern region of brazil) in 2013. here we report detection of the blaoxa-370 gene in 24 isolates belonging to three enterobacteriaceae species (22 klebsiella pneumoniae isolates, 1 enterobacter cloacae isolate, and 1 enterobacter aerogenes isolate) collected from five hospitals i ...201525987619
characterization of tn3000, a transposon responsible for blandm-1 dissemination among enterobacteriaceae in brazil, nepal, morocco, and enterobacteriaceae, the blandm genes have been found in many different genetic contexts, and a wide diversity of plasmid scaffolds bearing those genes has been found. in august 2013, we identified ndm-1-producing escherichia coli and enterobacter hormaechei strains from a single rectal swab sample from a patient hospitalized in rio de janeiro, brazil, who had no history of travel abroad. complete dna sequencing using the illumina platform and annotation of the two plasmids harboring the bland ...201526392506
effects of ensiling fermentation and aerobic deterioration on the bacterial community in italian ryegrass, guinea grass, and whole-crop maize silages stored at high moisture content.the effects of storage period and aerobic deterioration on the bacterial community were examined in italian ryegrass (ir), guinea grass (gg), and whole-crop maize (wm) silages. direct-cut forages were stored in a laboratory silo for 3, 7, 14, 28, 56, and 120 d without any additives; live counts, content of fermentation products, and characteristics of the bacterial community were determined. 2,3-butanediol, acetic acid, and lactic acid were the dominant fermentation products in the ir, gg, and w ...201325049913
chromosomal cephalosporinase in enterobacter hormaechei as an ancestor of act-1 plasmid-mediated ampc β this study of the diversity of ampc β-lactamase in clinical isolates of enterobacter spp., a strain was found carrying the plasmid-mediated ampc β-lactamase act-1 gene on its chromosome. the strain was identified as enterobacter hormaechei using phylogenetic analysis of 16s rrna and hsp60 genes. in addition, the species was confirmed by dna-dna hybridization. the genetic environment of the bla(act-1) gene was characterized, including the ampr and ampg genes, using a two-step pcr. the amino ac ...201221873382
resistance patterns and integron cassette arrays of enterobacter cloacae complex strains of human origin.the aim of this research was to analyse the resistance patterns and characterize the distribution and genetic content of resistance integrons within enterobacter cloacae complex strains originating from hospitalized patients. the strains were included in the e. cloacae complex study following sequence analysis of the hsp60 gene. the determination of resistance towards eight classes of antimicrobials was followed by pcr detection of integrons and analyses of the size and sequences of their variab ...201121330416
global molecular epidemiology of imp-producing data on the molecular epidemiology of enterobacteriaceae with imp carbapenemases are lacking. we performed short-read (illumina) whole-genome sequencing on a global collection of 38 imp-producing clinical enterobacteriaceae (2008 to 2014). imp-producing enterobacteriaceae (7 varieties within 11 class 1 integrons) were mainly present in the south pacific and asia. specific blaimp-containing integrons (in809 with blaimp-4, in722 with blaimp-6, and in687 with blaimp-14) were circulati ...201728167555
enterobacter muelleri sp. nov., isolated from the rhizosphere of zea mays.a beige-pigmented, oxidase-negative bacterial strain (jm-458t), isolated from a rhizosphere sample, was studied using a polyphasic taxonomic approach. cells of the isolate were rod-shaped and stained gram-negative. a comparison of the 16s rrna gene sequence of strain jm-458t with sequences of the type strains of closely related species of the genus enterobacter showed that it shared highest sequence similarity with enterobacter mori (98.7 %), enterobacter hormaechei (98.3 %), enterobacter cloaca ...201526294947
enterobacter xiangfangensis sp. nov., isolated from chinese traditional sourdough, and reclassification of enterobacter sacchari zhu et al. 2013 as kosakonia sacchari comb. nov.a gram-stain-negative bacterial strain, 10-17(t), was isolated from traditional sourdough in heilongjiang province, china. the bacterium was characterized by a polyphasic approach, including 16s rrna gene sequence analysis, rna polymerase β subunit (rpob) gene sequence analysis, dna gyrase (gyrb) gene sequence analysis, initiation translation factor 2 (infb) gene sequence analysis, atp synthase β subunit (atpd) gene sequence analysis, fatty acid methyl ester analysis, determination of dna g+c co ...201424824638
isolation, screening, characterization, and selection of superior rhizobacterial strains as bioinoculants for seedling emergence and growth promotion of mandarin orange (citrus reticulata blanco).mandarin orange (mo) is an important fruit crop of tropical and subtropical regions of the world. a total of 217 morphologically distinct rhizobacteria from mo orchards in 3 states of northeastern india were isolated and analyzed for 4 plant-growth-promoting (pgp) attributes: nitrogen fixation, production of indole acetic acid like substances, solubilization of phosphate, and ability to antagonize pathogenic fungi. isolates were ranked based on in-vitro-assayed pgp attributes, and 10 superior is ...201424498985
enterobacter cloacae complex: clinical impact and emerging antibiotic resistance.species of the enterobacter cloacae complex are widely encountered in nature, but they can act as pathogens. the biochemical and molecular studies on e. cloacae have shown genomic heterogeneity, comprising six species: enterobacter cloacae, enterobacter asburiae, enterobacter hormaechei, enterobacter kobei, enterobacter ludwigii and enterobacter nimipressuralis, e. cloacae and e. hormaechei are the most frequently isolated in human clinical specimens. phenotypic identification of all species bel ...201222827309
microbial biofilm development on neonatal enteral feeding tubes.neonates in intensive care units often require supporting medical devices and antibiotic treatment. the intensive care treatment combined with their immature immune system, the increased permeability of mucosa, and the undeveloped microflora of the gut may render the neonates highly vulnerable to colonisation and subsequent infections when exposed to opportunistic pathogens. these infections may not only be local gastrointestinal infections, but also systematic following translocation from the g ...201525366224
phytochemical screening: antioxidant and antibacterial properties of potamogeton species in order to obtain valuable feed additives.the alcoholic extracts from three submerged perennial plants potamogeton crispus l., p. pusillus l. and p. pectinatus l. were analyzed by gas chromatography-mass spectrometry coupled with solid phase microextraction (spme-gc/ms) and by high performance liquid chromatography (hplc) and their volatile fingerprint and polyphenols composition was mutually compared. twenty-nine chemical compounds were detected and identified in ethanolic and methanolic extracts; the highest abundance (over 5%) in des ...201526369590
coproduction of ndm-1 and kpc-2 in enterobacter hormaechei from brazil.the most important resistance mechanism against β-lactam drugs is the production of carbapenemases. in this study, we report the first identification of klebsiella pneumoniae carbapenemase (kpc)-2 and new delhi metallo-β-lactamase (ndm)-1 in enterobacter hormaechei subps. oharae from brazil. the detection of carbapenemases was done by phenotypic assays, pcr, and dna sequencing, whereas the identification was performed by conventional techniques, sequencing of the 16s rdna gene, and hsp60-genotyp ...201525473727
molecular characteristics of carbapenem-resistant gram-negative bacteria in southern china.a total of 368 nonreplicate gram-negative bacteria with resistance to imipenem or meropenem were collected to search for carbapenemase genes, class 1 integrons, and insertion sequence with common region 1 (iscr1). the carbapenemase genes blaimp-4, blakpc-2, and blandm-1 were found in two enterobacteriaceae and seven pseudomonas aeruginosa isolates, nine klebsiella pneumoniae isolates, and seven enterobacteriaceae and two acinetobacter spp. isolates. the class d oxa-type carbapenemase genes blaox ...201525469995
artificial citrate operon and vitreoscilla hemoglobin gene enhanced mineral phosphate solubilizing ability of enterobacter hormaechei dhrss.mineral phosphate solubilization by bacteria is mediated through secretion of organic acids, among which citrate is one of the most effective. to overproduce citrate in bacterial systems, an artificial citrate operon comprising of genes encoding nadh-insensitive citrate synthase of e. coli and salmonella typhimurium sodium-dependent citrate transporter was constructed. in order to improve its mineral phosphate solubilizing (mps) ability, the citrate operon was incorporated into e. hormaechei dhr ...201425016342
detection of ndm-1-, ctx-m-15-, and qnrb4-producing enterobacter hormaechei isolates in brazil. 201424449772
culture-independent identification of bacteria associated with ovine 'broken mouth' periodontitis.'broken mouth' periodontitis (bmp) is a painful condition of sheep grazed on rough pasture and involves periodontal infection of the incisor teeth and progressive tooth loss. this can reduce the efficiency of grazing of sheep, which contributes to malnutrition, weight loss, systemic health problems, poor quality of life and early culling from flocks. consequently, this condition is a major economic problem to sheep farmers. however, there are no treatment or control methods available. the aim of ...201323928119
prevalence and characterization of multidrug-resistant zoonotic enterobacter spp. in poultry of bangladesh.poultry and poultry products are major contributors of zoonotic pathogens. limited data are available on enterobacter spp. as a potent zoonotic pathogen in poultry. the present study is a first endeavor on the emergence of multidrug-resistant zoonotic enterobacter spp. and its prevalence arising from poultry in bangladesh. cloacal swabs from poultry samples of five different farms at savar, dhaka, bangladesh were collected and from 106 isolates, 18 presumptive enterobacter spp. were obtained. an ...201323560422
detection of oxa-370, an oxa-48-related class d β-lactamase, in enterobacter hormaechei from brazil. 201424709259
reevaluation of a suspected cronobacter sakazakii outbreak in 2010, two infants became ill at a hospital in mexico. subsequently, a range of clinical, environmental, and powdered and rehydrated infant formula isolates were identified by using a combination of phenotyping and pcr probes. the strains were clustered according to pulsed-field gel electrophoresis. the causative agent was reported as cronobacter sakazakii, with powdered infant formula (pif) identified as the likely source of the infections. this new study further characterized the isolates fr ...201526038912
proteomic response of β-lactamases-producing enterobacter cloacae complex strain to cefotaxime-induced stress.bacteria of the enterobacter cloacae complex are among the ten most common pathogens causing nosocomial infections in the usa. consequently, increased resistance to β-lactam antibiotics, particularly expanded-spectrum cephalosporins like cefotaxime (ctx), poses a serious threat. differential in-gel electrophoresis (dige), followed by lc-ms/ms analysis and bioinformatics tools, was employed to investigate the survival mechanisms of a multidrug-resistant e. hormaechei subsp. steigerwaltii 51 carry ...201627162211
rapid identification of enterobacter hormaechei and enterobacter cloacae genetic cluster iii.enterobacter cloacae complex bacteria are of both clinical and environmental importance. phenotypic methods are unable to distinguish between some of the species in this complex, which often renders their identification incomplete. the goal of this study was to develop molecular assays to identify enterobacter hormaechei and ent. cloacae genetic cluster iii which are relatively frequently encountered in clinical material.201424428402
metal oxide gas sensors, a survey of selectivity issues addressed at the sensor lab, brescia (italy).this work reports the recent results achieved at the sensor lab, brescia (italy) to address the selectivity of metal oxide based gas sensors. in particular, two main strategies are being developed for this purpose: (i) investigating different sensing mechanisms featuring different response spectra that may be potentially integrated in a single device; (ii) exploiting the electronic nose (en) approach. the former has been addressed only recently and activities are mainly focused on determining th ...201728353673
bioconversion of lutein by enterobacter hormaechei to form a new compound, 8-methyl-α investigate the final product of the bioconversion of lutein by a novel lutein-degrading bacterium, enterobacter hormaechei a20, and the kinetics of the process.201728353147
metabolic and co-metabolic transformation of diclofenac by enterobacter hormaechei d15 isolated from activated sludge.the presence of non-steroidal anti-inflammatory drugs, such as diclofenac (dcf), in the environment, is an emerging problem due to their harmful effects on non-target organisms, even at low concentrations. we studied the biodegradation of dcf by the strain d15 of enterobacter hormaechei. the strain was isolated from an activated sludge, and identified as e. hormaechei based on its physiological characteristics and its 16 s rna sequence. using hptlc and gc-ms methods, we demonstrated that this st ...201728175958
molecular identification of multi drug resistant bacteria from urinary tract infected urine samples.urinary tract infections (utis) are of great concern in both developing and developed countries all over the world. even though the infections are more common in women and children, they are at a considerable rate in men and of all ages. the uropathogens causing the infections are spread through various routes. the treatment generally recommended by the physicians is antibiotic usage. but, most of the uropathogens have evolved antibiotic resistance mechanisms. this makes the present situation he ...201627354209
inoculation of paperboard mill sludge versus mixed culture bacteria for hydrogen production from paperboard mill wastewater.a comparative evaluation of paperboard mill sludge (pms) versus mixed culture bacteria (mcb) as inoculum for hydrogen production from paperboard mill wastewater (pmw) was investigated. the experiments were conducted at different initial cultivation phs, inoculums to substrate ratios (isrs gvs/gcod), and hydraulic retention times (hrts). the peak hydrogen yield (hy) of 5.29 ± 0.16 and 1.22 ± 0.11 mmol/gcodinitial was occurred at ph = 5 for mcb and pms, respectively. at ph of 5, the hy and cod rem ...201626498965
genomic epidemiology of global vim-producing data on the molecular epidemiology of enterobacteriaceae with vim carbapenemases are limited.201728520983
intestinal bacterial communities of trypanosome-infected and uninfected glossina palpalis palpalis from three human african trypanomiasis foci in cameroon.glossina sp. the tsetse fly that transmits trypanosomes causing the human or the animal african trypanosomiasis (hat or aat) can harbor symbiotic bacteria that are known to play a crucial role in the fly's vector competence. we hypothesized that other bacteria could be present, and that some of them could also influence the fly's vector competence. in this context the objectives of our work were: (a) to characterize the bacteria that compose the g. palpalis palpalis midgut bacteriome, (b) to evi ...201728824591
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