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siderophore typing, a powerful tool for the identification of fluorescent and nonfluorescent pseudomonads.a total of 301 strains of fluorescent pseudomonads previously characterized by conventional phenotypic and/or genomic taxonomic methods were analyzed through siderotyping, i.e., by the isoelectrophoretic characterization of their main siderophores and pyoverdines and determination of the pyoverdine-mediated iron uptake specificity of the strains. as a general rule, strains within a well-circumscribed taxonomic group, namely the species pseudomonas brassicacearum, pseudomonas fuscovaginae, pseudo ...200212039729
pseudomonas koreensis sp. nov., pseudomonas umsongensis sp. nov. and pseudomonas jinjuensis sp. nov., novel species from farm soils in korea.among pseudomonas strains isolated from korean agricultural soils, four strains (ps 9-14 group: ps 1-2, ps 1-10, ps 5-5 and ps 9-14t) from the suwon, goesan and samchok regions, three strains (ps 3-10 group: ps 2-22, ps 3-1 and ps 3-10t) from umsong region and four strains (pss 26 group: pss 14, pss 25, pss 26t and pss 27) from jinju region were identified as three independent groups on the basis of 16s rdna sequence analysis. while, on the basis of 16s rdna sequence analysis, ps 9-14t and ps 3- ...200312656147
pseudomonas rhizosphaerae sp. nov., a novel species that actively solubilizes phosphate in vitro.a bacterial strain (designated ih5(t)), isolated from rhizospheric soil of grasses growing spontaneously in spanish soil, actively solubilized phosphates in vitro when bicalcium phosphate was used as a phosphorus source. this strain was gram-negative, strictly aerobic, rod-shaped and motile. the strain produced catalase, but not oxidase. cellulose, casein, starch, gelatin, aesculin and urea were not hydrolysed. growth was observed with many carbohydrates as the carbon source. the main non-polar ...200314657147
pseudomonas lutea sp. nov., a novel phosphate-solubilizing bacterium isolated from the rhizosphere of grasses.a phosphate-solubilizing bacterial strain designated ok2(t) was isolated from rhizospheric soil of grasses growing spontaneously in a soil from spain. cells of the strain were gram-negative, strictly aerobic, rod-shaped and motile. phylogenetic analysis of the 16s rrna gene indicated that this bacterium belongs to the gamma-subclass of proteobacteria within the genus pseudomonas and that the closest related species is pseudomonas graminis. the strain produced catalase but not oxidase. cellulose, ...200415143034
isolation, characterization, and effect of fluorescent pseudomonads on micropropagated sugarcane.in this study, we report on the isolation, identification, and characterization of seven fluorescent pseudomonads isolated from the roots, shoots, and rhizosphere soil of sugarcane and their impacts on the growth of sugarcane plantlets. 16s rrna gene sequence of five isolates showed close homology with pseudomonas putida, one with pseudomonas graminis, and one with pseudomonas fluorescens. physiological and biochemical characterizations were determined using api50ch and qts24 identification kits ...200919898541
whole-genome sequence of pseudomonas graminis strain uasws1507, a potential biological control agent and biofertilizer isolated in switzerland.we report here the whole-genome shotgun sequence of the strain uasws1507 of the species pseudomonas graminis, isolated in switzerland from an apple tree. this is the first genome registered for this species, which is considered as a potential and valuable resource of biological control agents and biofertilizers for agriculture.201627795260
antagonistic potential of pseudomonas graminis 49m against erwinia amylovora, the causal agent of fire blight.in a previous study (mikiciƄski et al. in eur j plant pathol, doi: 10.1007/s10658-015-0837-y , 2015), we described the characterization of novel strain 49m of pseudomonas graminis, isolated from the phyllosphere of apple trees in poland showing a good protective activity against fire blight on different organs of host plants. we now report investigations to clarify the basis for this activity. strain 49m was found to produce siderophores on a medium containing complex cas-fe(3+) and hdtma, but w ...201627002332
biopreservative methods to control the growth of foodborne pathogens on fresh-cut lettuce.fruits and vegetables can become contaminated by foodborne pathogens such as escherichia coli o157:h7, salmonella and listeria monocytogenes, and it has been demonstrated that current industrial sanitizing treatments do not eliminate the pathogens when present. chemical control is widely used, but biological control appears to be a better solution, mainly using the native microbiota present on fresh produce. the first objective of this study was to isolate native microbiota from whole and fresh- ...201526210531
antagonistic effect of pseudomonas graminis cpa-7 against foodborne pathogens in fresh-cut apples under simulated commercial conditions.recently, we reported that the application of the strain cpa-7 of pseudomonas graminis, previously isolated from apple, could reduce the population of foodborne pathogens on minimally processed (mp) apples and peaches under laboratory conditions. therefore, the objective of the present work was to find an antioxidant treatment and a packaging atmosphere condition to improve cpa-7 efficacy in reducing a cocktail of four salmonella and five listeria monocytogenes strains on mp apples under simulat ...201323200645
draft genome sequence of pseudomonas graminis pdd-13b-3, a model strain isolated from cloud water.the whole genome of pseudomonas graminis pdd-13b-3, a strain of bacteria isolated from cloud water, was sequenced. this showed that this microorganism is equipped with genes that could potentially be involved in its survival in the atmosphere and clouds: those for oxidative stress and carbon starvation responses, dna repair, and iron uptake.201728663290
control of foodborne pathogens on fresh-cut fruit by a novel strain of pseudomonas graminis.the consumption of fresh-cut fruit has substantially risen over the last few years, leading to an increase in the number of outbreaks associated with fruit. moreover, consumers are currently demanding wholesome, fresh-like, safe foods without added chemicals. as a response, the aim of this study was to determine if the naturally occurring microorganisms on fruit are "competitive with" or "antagonistic to" potentially encountered pathogens. of the 97 and 107 isolates tested by co-inoculation with ...201323541207
pseudomonas japonica sp. nov., a novel species that assimilates straight chain alkylphenols.a bacterial strain, wl(t), which was isolated from an activated sludge, was able to degrade alkylphenols. 16s rdna sequence analysis indicated that strain wl(t) belonged to the genus pseudomonas (sensu stricto) and formed a monophyletic clade with the type strain of pseudomonas graminis and other members in the pseudomonas putida subcluster with sequence similarity values higher than 97%. genomic relatedness based on dna-dna hybridization of strain wl(t) to these strains is 2-41%. strain wl(t) c ...200818323682
a taxonomic study of bacteria isolated from grasses: a proposed new species pseudomonas graminis sp. nov.the taxonomic position of a yellow-pigmented group of bacteria, isolated from the phyllosphere of grasses was investigated. results obtained from restriction analysis of amplified 16s rdna with seven endonucleases (cfoi, haeiii, alui, hinfi, mspi, sau3a and scrfi) showed identical restriction patterns for each enzyme of all isolates studied, which suggests that all strains belong to the same species. the grass isolates displayed the characteristics of the genus pseudomonas. they were gram-negati ...199910028275
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