| terminal structure involving a single-stranded stretch in the double-stranded rna from penicillium chrysogenum virus. | | 1977 | 408972 |
| intracellular appearance of penicillium chrysogenum virus. | | 1973 | 4126408 |
| comparison of interferon induction in mice by purified penicillium chrysogenum virus and derived double-stranded rna. | | 1971 | 5001254 |
| deep sequencing analysis of viruses infecting grapevines: virome of a vineyard. | double stranded rna, isolated from 44 pooled randomly selected vines from a diseased south african vineyard, has been used in a deep sequencing analysis to build a census of the viral population. the dsrna was sequenced in an unbiased manner using the sequencing-by-synthesis technology offered by the illumina genome analyzer ii and yielded 837 megabases of metagenomic sequence data. four known viral pathogens were identified. it was found that grapevine leafroll-associated virus 3 (glrav-3) is t ... | 2010 | 20172578 |
| comparison of the biophysical and biochemical properties of penicillium cyaneo-fulvum virus and penicillium chrysogenum virus. | the biophysical and biochemical properties of penicillium cyaneo-fulvum virus (pc-fv) and penicillium chrysogenum virus (pcv) have been compared. in sucrose density gradient sedimentation, purified virus preparations gave one major component, l, and three minor components e1, e2 and h with sedimentation coefficients of 145s, 80s, 102s and 172s respectively in each case. e1, e2 were shown to be empty particles. pc-fv l particles contained only double-stranded rna, which separated in polyacrylamid ... | 1977 | 833575 |
| action of nucleases on double-stranded rna. | double-stranded rnas from penicillium chrysogenum virus have been treated with rnase iii, pancreatic rnase a and rnase t1 and the degradation of the rnas has been studied under different conditions. it was found that only the two former enzymes cut across both strands, rnase t1 cannot cleave double strands. rnase iii was shown to digest double-stranded rna by a two step process: an initial phase of specific cleavage is followed by random degradation. in the first phase the enzyme exhibited a def ... | 1976 | 813998 |
| chrysovirus structure: repeated helical core as evidence of gene duplication. | chrysoviruses are double-stranded rna viruses with a multipartite genome. structure of two fungal chrysoviruses, penicillium chrysogenum virus and cryphonectria nitschkei chrysovirus 1, has been determined by three-dimensional cryo-electron microscopy analysis and in hydrodynamic studies. the capsids of both viruses are based on a t=1 lattice containing 60 subunits, remain structurally undisturbed throughout the viral cycle, and participate in genome metabolism. the capsid protein is formed by a ... | 2013 | 23498904 |
| cryo-em near-atomic structure of a dsrna fungal virus shows ancient structural motifs preserved in the dsrna viral lineage. | viruses evolve so rapidly that sequence-based comparison is not suitable for detecting relatedness among distant viruses. structure-based comparisons suggest that evolution led to a small number of viral classes or lineages that can be grouped by capsid protein (cp) folds. here, we report that the cp structure of the fungal dsrna penicillium chrysogenum virus (pcv) shows the progenitor fold of the dsrna virus lineage and suggests a relationship between lineages. cryo-em structure at near-atomic ... | 2014 | 24821769 |
| the t=1 capsid protein of penicillium chrysogenum virus is formed by a repeated helix-rich core indicative of gene duplication. | penicillium chrysogenum virus (pcv), a member of the chrysoviridae family, is a double-stranded rna (dsrna) fungal virus with a multipartite genome, with each rna molecule encapsidated in a separate particle. chrysoviruses lack an extracellular route and are transmitted during sporogenesis and cell fusion. the pcv capsid, based on a t=1 lattice containing 60 subunits of the 982-amino-acid capsid protein, remains structurally undisturbed throughout the viral cycle, participates in genome metaboli ... | 2010 | 20463071 |
| molecular characterization of penicillium chrysogenum virus: reconsideration of the taxonomy of the genus chrysovirus. | molecular cloning and complete nucleotide sequencing of penicillium chrysogenum virus (pcv) dsrnas indicated that pcv virions contained four dsrna segments with sizes of 3562, 3200, 2976 and 2902 bp. each dsrna segment had unique sequences and contained a single large open reading frame (orf). in vitro translation of transcripts derived from full-length cdna clones of pcv dsrnas yielded single products of sizes similar to those predicted from the deduced amino acid sequences of the individual or ... | 2004 | 15218197 |
| three-dimensional structure of penicillium chrysogenum virus: a double-stranded rna virus with a genuine t=1 capsid. | although double-stranded (ds) rna viruses are a rather diverse group, they share general architectural principles and numerous functional features. all dsrna viruses, from the mammalian reoviruses to the bacteriophage phi6, including fungal viruses, share a specialized capsid involved in transcription and replication of the dsrna genome, and release of the viral plus strand rna. this ubiquitous capsid consists of 120 protein subunits in a so-called t=2 organization. the stringent requirements of ... | 2003 | 12888349 |
| a circular dichroism study of the structure of penicillium chrysogenum mycovirus. | we have examined the absorption and circular dichroism spectra of intact penicillium chrysogenum virus, empty capsid particles, and isolated double-stranded rna. the absorbance at 260 nm of intact virus was less than 4% hypochromic relative to the absorbances of the free double-stranded rna and free viral protein, indicating very little change in the base stacking interactions of the rna. circular dichroism studies of intact virus indicate that the capsid protein consists of 45% alpha-helix. emp ... | 1983 | 6408610 |