| the humoral immune response of turbot, scophthalmus maximus l., to spore-surface antigens of microsporidian parasites. | experiments based on enzyme-linked immunosorbent assay (elisa) revealed considerable antigenic homology in turbot between two species of microsporidian, tetramicra brevifilum (a parasite of the turbot, scophthalmus maximus) and glugea caulleryi (a parasite of the lesser sand-eel, ammodytes tobianus). we next investigated whether g. caulleryi is able to suppress the turbot immune response. intraperitoneal inoculation of turbot with g. caulleryi spores (whether heat-killed or viable) did not suppr ... | 1996 | 9014320 |
| tetramicra brevifilum (matthews & matthews, 1980) (microsporida: tetramicriidae) in a new fish host, lophius budegassa (spinola, 1807) in spain. | tetramicra brevifilum, a microsporidian parasite of scophthalmus maximus, was found in lophius budegassa for the first time. this parasite was detected in 5 of 199 hosts captured in the coastal waters of barcelona (northwest mediterranean), which enlarges the geographic distribution of this microsporidian. affected fish did not show any external sign of disease, and cysts of t. brevifilum were found associated with the body musculature but were easily differentiated from those of spraguea lophii ... | 1998 | 9521010 |
| dna probes for detection of the fish microsporidians microgemma caulleryi and tetramicra brevifilum. | dna probes were developed for the detection and identification of 2 microsporidian parasites of marine fishes, microgemma caulleryi (infecting the liver of the greater sand-eel, hyperoplus lanceolatus) and tetramicra brevifilum (infecting muscle, intestine and liver of the turbot, scophthalmus maximus, a commercially important species). the probe-development procedure used is fast and straightforward, and readily applicable to the development of probes for other microsporidian species. first, ge ... | 1999 | 10503252 |
| rflp analysis of pcr-amplified small subunit ribosomal dna of three fish microsporidian species. | the phylogenetic relationships of the microsporidian species microgemma caulleryi, pleistophora finisterrensis and tetramicra brevifilum were investigated on the basis of restriction fragment length polymorphism (rflp) analysis of pcr-amplified small-subunit rdna (ssurdna). using pcr primers specific for microsporidian ssurdna, a single product was obtained from each species, and heteroduplex analysis indicated a high degree of sequence homology among the 3 products. in rflp analysis of the pcr- ... | 2000 | 10726273 |
| effect of tetramicra brevifilum (microspora) infection on respiratory-burst responses of turbot (scophthalmus maximus l.) phagocytes. | in vitro assays were performed to investigate microsporidian-induced intracellular and extracellular production of reactive oxygen species (ros) by peritoneal-exudate adherent (pea) cells from turbot. ros production was quantified using the fluorescent reagents oxyburst green h2hff bsa (extracellular) and oxyburst green h2dcfda succinimidyl ester (intracellular). five days before assay, the cells had been elicited in vivo by intraperitoneal injection of sodium thioglycollate or spores of tetrami ... | 2001 | 11592590 |
| non-isotopic detection of tetramicra brevifilum (microspora) dna in turbot tissues. | a non-isotopic in situ hybridization (ish) method was developed for detection of tetramicra brevifilum, a commercially important parasite in farmed turbot scophthalmus maximus. the probe relies on sequences from the small-subunit rrna gene (ssurdna) of tetramicra brevifilum and was obtained by polymerase chain reaction then labeled with digoxigenin. the results obtained demonstrate that the probe hybridizes well with genomic dna of the spores; thus, it is an effective method for detecting multio ... | 2001 | 11780847 |
| pcr detection of tetramicra brevifilum (microspora) infection in turbot (scophthalmus maximus l.) musculature. | this study investigated the spatial distribution of tetramicra brevifilum spores in the musculature of infected turbot scophthalmus maximus, with the aim of identifying the most appropriate body locations for diagnostic assays. a pcr protocol optimized for the detection of t. brevifilum spores in turbot muscle is also described. in fish showing low- and moderate-intensity infection, the spatial distribution of spores was best fitted by a negative binomial distribution, indicating a clumped spati ... | 2002 | 11860032 |
| the role of opsonization by antibody and complement in in vitro phagocytosis of microsporidian parasites by turbot spleen cells. | we investigated the role played by opsonization by antibody and complement in in vitro phagocytosis of microsporidian spores by turbot adherent phagocytes. most turbot adherent cells displaying phagocytic activity are probably macrophages. phagocytosis of yeast cells and polystyrene beads was greatly enhanced in the presence of both the ig and the non-ig (i.e. complement-containing) fractions of normal turbot serum, but phagocytosis of glugea caulleryi or tetramicra brevifilum spores was not aff ... | 1996 | 8797289 |
| fast real-time pcr assay for detection of tetramicra brevifilum in cultured turbot. | global aquaculture production of turbot has rapidly increased worldwide in the last decade and it is expected to have even bigger growth in the next years due to new farms operating. the losses caused by pathogen infections have grown at the same time as the production of this species. parasitological infections are among the main relevant pathologies associated with its culture and produce serious losses in aquaculture, reduce the growth rate in fish and may lead to unmarketable fish due to ske ... | 2013 | 23068417 |
| first record of tetramicra brevifilum in lumpfish (cyclopterus lumpus, l.). | a microsporidian species with 98.3-98.4% nucleotide identity to tetramicra brevifilum (journal of fish diseases, 3, 1980, 495) was diagnosed in lumpfish (cyclopterus lumpus, l.) broodstock held at a breeding and rearing facility in western ireland. the fish were wild-caught from the west coast of ireland, and the first case was diagnosed one year after capture. clinical signs included severe bloating, lethargy, exophthalmos, anorexia, white patches on the cornea and externally visible parasitic ... | 2016 | 27716959 |
| examination of naturally exposed bottlenose dolphins (tursiops truncatus) for microsporidia, cryptosporidium, and giardia. | bottlenose dolphins (tursiops truncatus) captured in the estuarine waters off the coasts of south carolina and florida were examined for the presence of microsporidia, cryptosporidium sp., and giardia sp. dna extracted from feces or rectal swabs was amplified by polymerase chain reaction using parasite-specific small subunit ribosomal rna gene primers. all positive specimens were subjected to gene sequence analysis. of 83 dolphins, 17 were positive for microsporidia. none was positive for crypto ... | 2008 | 18372633 |