Publications

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parasitic protozoa of cyprinid fishes: protozoa of the roach rutilus rutilus (linnaeus, 1758) in czechoslovakia.in the years 1985-1988, a total of 254 specimens of the roach, rutilus rutilus, captured in 23 localities of south bohemia, were examined for the presence of protozoan parasites. only 17 specimens (6.7%) were free of infection, whereas the others were infected at least with one parasite species, mixed infections were observed most frequently. the following species were found rarely: myxidium rhodei léger, 1905 in the liver and muscles. pleistophora mirandellae vaney et conte, 1901 in ovaries, tr ...19911916526
ultrastructure of the wall of the sporophorous vesicle during sporogony of pleistophora mirandellae (protozoa:microspora).the wall of the younger sporophorous vesicle (enclosing the sporogogonial plasmodium) of pleistophora mirandellae vaney and conte, 1901 is composed of a membranous labyrinth, about 0.3-0.6 microns high, interwoven with host cell cytoplasm. there is electron-opaque matter within the partitions of the labyrinth. at the onset of plasmotomy, the partitions of the labyrinth become shorter and then possibly due to stretching of the wall and withdrawal of the membranes, the electron-opaque matter is ej ...19968897511
fatty acid composition of four microsporidian species compared to that of their host fishes.the fatty acid composition of four microsporidian species (glugea atherinae, spraguea lophii, glugea americanus, and pleistophora mirandellae) and their host fishes has been determined using gas chromatography. twenty-four fatty acids were identified with differences in relative abundance of fatty acids among the four parasites. certain even-saturated fatty acids were found in a very high proportion: palmitic acid (16:0) represented one-third of total fatty acids in pleistophora mirandellae. the ...200010651288
small subunit ribosomal dna phylogeny of microsporidia with particular reference to genera that infect fish.molecular data have proved useful in the study of microsporidia phylogeny. previous studies have shown that there are several important differences between phylogenies based on rrna and morphological data. in the present study, small subunit (ssu) rdna sequences were obtained from 7 different fish-infecting microsporidia from 4 different genera (glugea thélohan, 1891, loma morrison and sprague, 1981, pleistophora gurley, 1893, and spraguea weissenberg, 1976). the lengths of the ssu rdna genes in ...200010701575
relationships of microsporidian genera, with emphasis on the polysporous genera, revealed by sequences of the largest subunit of rna polymerase ii (rpb1).molecular data have proved useful as an alternative to morphological data in showing the relationships of genera within the phylum microsporidia, but until now have been available only for ribosomal genes. in previous studies protein-coding genes of microsporidia have been used only to assess their position in the evolution of eukaryotes. for the first time we report on the use of a protein-coding gene, the a-g region of the largest subunit of rna polymerase ii (rpb1) from 14 mainly polysporous ...200111249186
ovipleistophora gen. n., a new genus for pleistophora mirandellae-like microsporidia.based on ultrastructural study and molecular analysis, a new genus, ovipleistophora, is established for pleistophora mirandellae-like microsporidia from roach and ruff oocytes. unlike pleistophora, ovipleistophora has a thick additional envelope around the meront. this envelope breaks open to release the cells into the host cell cytoplasm. the cells, becoming multinuclear sporogonic plasmodia, already have a surface coat that transforms into the sporont wall and eventually into the sporophorous ...200212005235
intersex in wild roach (rutilus rutilus) from danish sewage effluent-receiving streams.roach (rutilus rutilus) from danish streams that receive discharges of domestic sewage effluent were examined for the presence of alterations to gonadal development. in male roach, intersex was observed at a prevalence of 4.5-5% at reference sites and 6.7-6.5% at sewage effluent-impacted sites. a positive correlation was found between sewage effluent load and intersex frequency among male roach. the highest frequency of intersex (26.5%) was found in the stream kristrup landkanal, which had the h ...200616054215
pleistophora infestation in fathead minnows, pimephales promelas (rafinesque).pleistophora infestation was observed in adult fathead minnows, pimephales promelas, held under laboratory conditions. fish were clinically healthy, and presented no gross findings at necropsy. histopathology revealed parasitic stages only in the ovaries. spores within sporophorous vesicles were mainly encountered in late vitellogenic oocytes and were ultrastructurally identified as a microsporidian parasite. heavily parasitized oocytes underwent degeneration followed by the release of spores in ...200516303025
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