| bacillus shackletonii sp. nov., from volcanic soil on candlemas island, south sandwich archipelago. | a sample of mossy soil taken from the eastern lava flow of northern candlemas island, south sandwich archipelago, yielded six isolates of aerobic, endospore-forming bacteria. miniaturized routine phenotypic tests and other observations, amplified rdna restriction analysis and sds-page analysis suggested that the strains represent a novel taxon. 16s rdna sequence comparisons support the proposal of a novel species, bacillus shackletonii sp. nov., the type strain of which is lmg 18435(t) (=cip 107 ... | 2004 | 15023945 |
| molecular characterisation of bacterial contamination in semi-final gelatine extracts, using denaturing gradient gel electrophoresis. | contamination of gelatine may affect the safety and/or quality of its applications. characterisation of bacterial isolates from semi-final gelatine batches revealed thermotolerant, aerobic, endosporeforming contaminants. in this paper, bacterial contamination in gelatine batches is analysed without previous isolation, by means of denaturing gradient gel electrophoresis (dgge) of pcr-amplified 16s rdna sequences. v9 and v6-v8 regions of the 16s rdna gene were found more suitable for this purpose ... | 2004 | 15490563 |
| bacillus isabeliae sp. nov., a halophilic bacterium isolated from a sea salt evaporation pond. | a low-g+c, gram-positive isolate, designated strain cvs-8(t), was isolated from a sea salt evaporation pond on the island of sal in the cape verde archipelago. this organism was found to be a catalase- and oxidase-positive, non-motile, spore-forming, aerobic, curved rod-shaped organism with an optimum growth temperature of about 35-37 degrees c and an optimum ph between 7.5 and 8.0. optimal growth occurred in media containing 4-6% (w/v) nacl and no growth occurred in medium without nacl. the cel ... | 2008 | 18175713 |
| bacillus ectoiniformans sp. nov., a halotolerant bacterium isolated from sediments of south china sea. | a halotolerant gram-positive bacterium, which was isolated from sediment samples of south china sea, was subjected to a taxonomic study. the strain ne-14t, grew well at a wide temperature and ph ranges, 10.0-45.0 °c and 6-10, with an optimum at 30 °c and ph 8.0, respectively. the growth of strain ne-14t was occurred at total salt concentrations between 0-10% (w/v) with optimum at 2% (w/v). phylogenetic analysis based on the 16s rrna sequence comparison indicated that the isolate belongs to the g ... | 2015 | 26559004 |
| bacillus mesophilus sp. nov., an alginate-degrading bacterium isolated from a soil sample collected from an abandoned marine solar saltern. | a novel gram-stain positive, endospore-forming bacterium, designated sa4(t), was isolated from a soil sample collected from an abandoned marine solar saltern at wendeng, shandong province, pr china. cells were observed to be rod shaped, alginase positive, catalase positive and motile. the strain was found to grow at temperatures ranging from 15 to 40 °c (optimum 35 °c), and ph 5.0-11.0 (optimum ph 8.0) with 0-7.0 % (w/v) nacl concentration (optimum nacl 3.0 %). phylogenetic analysis based on 16s ... | 2016 | 27084709 |
| bacillus dabaoshanensis sp. nov., a cr(vi)-tolerant bacterium isolated from heavy-metal-contaminated soil. | a cr(vi)-tolerant, gram-staining-positive, rod-shaped, endospore-forming and facultative anaerobic bacterium, designated as gss04(t), was isolated from a heavy-metal-contaminated soil. strain gss04(t) was cr(vi)-tolerant with a minimum inhibitory concentration of 600 mg l(-1) and was capable of reducing cr(vi) under both aerobic and anaerobic conditions. growth occurred with presence of 0-3 % (w/v) nacl (optimum 1 %), at ph 5.5-10.0 (optimum ph 7.0) and 15-50 °c (optimum 30-37 °c). the main resp ... | 2015 | 25603996 |
| bacillus pervagus sp. nov. and bacillus andreesenii sp. nov., isolated from a composting reactor. | two strains, 8-4-e12(t) and 8-4-e13(t), were isolated from a biowaste composting reactor. based on 16s rrna gene sequences, both strains belong to the genus bacillus. strain 8-4-e12(t) was most closely related to the type strains of bacillus shackletonii, b. acidicola, b. sporothermodurans and b. oleronius (96.4, 96.3, 96.0 and 95.6 % 16s rrna gene similarity, respectively), whereas strain 8-4-e13(t) was most closely related to the type strain of bacillus humi (96.5 % sequence similarity). strai ... | 2014 | 24021730 |
| novel aflatoxin-degrading enzyme from bacillus shackletonii l7. | food and feed contamination by aflatoxin (af)b₁ has adverse economic and health consequences. afb₁ degradation by microorganisms or microbial enzymes provides a promising preventive measure. to this end, the present study tested 43 bacterial isolates collected from maize, rice, and soil samples for afb₁-reducing activity. the higher activity was detected in isolate l7, which was identified as bacillus shackletonii. l7 reduced afb₁, afb₂, and afm₁ levels by 92.1%, 84.1%, and 90.4%, respectively, ... | 2017 | 28098812 |
| native phytoremediation potential of urtica dioica for removal of pcbs and heavy metals can be improved by genetic manipulations using constitutive camv 35s promoter. | although stinging nettle (urtica dioica) has been shown to reduce hm (heavy metal) content in soil, its wider phytoremediation potential has been neglected. urtica dioica was cultivated in soils contaminated with hms or polychlorinated biphenyls (pcbs). after four months, up to 33% of the less chlorinated biphenyls and 8% of hms (zn, pb, cd) had been removed. bacteria were isolated from the plant tissue, with the endophytic bacteria bacillus shackletonii and streptomyces badius shown to have the ... | 2016 | 27930707 |
| draft genome sequence of bacillus shackletonii lmg 18435t, isolated from volcanic mossy soil. | bacillus shackletonii lmg 18435(t) is a gram-positive, aerobic, and spore-forming bacterium. here, we report the 5.30-mb draft genome sequence of b. shackletonii lmg 18435(t), which will promote its fundamental research and provide useful information for genomic taxonomy and phylogenomics of bacillus-like bacteria. | 2016 | 26847895 |
| isolation and characterization of a thermophilic bacillus shackletonii k5 from a biotrickling filter for the production of polyhydroxybutyrate. | polyhydroxyalkanoates (phas) are aliphatic polyesters accumulated intracellularly by both gram-negative and gram-positive bacteria. however, compared to the phas of gram-negative bacteria, few endotoxins (lipopolysaccharides, lps), which would be co-purified with phas and cause immunogenic reactions, are found in the phas produced by gram-positive bacteria. a thermophilic gram-positive bacterium k5, which exhibited good growth and polyhydroxybutyrate (phb)-accumulating ability, has been isolated ... | 2014 | 25079994 |