| prevention of thromboses in human patients with bothrops lanceolatus envenoming in martinique: failure of anticoagulants and efficacy of a monospecific antivenom. research group on snake bites in martinique. | envenomation by the bothrops lanceolatus, a snake found only in martinique, leads to swelling and pain, and occasionally to systemic signs and/or coagulopathy. severe thromboses at some distance from the site of the bite may appear within 48 hr. uncertainties as to the actual development of thrombotic complications in patients appearing to be suffering from moderate poisoning and as to the availability and the toxicity of a monospecific antivenom (avs) initially led us to reserve antivenom for t ... | 1995 | 7771608 |
| purification of an acidic phospholipase a2 from bothrops lanceolatus (fer de lance) venom: molecular and enzymatic properties. | the acidic phospholipase a2 from bothrops lanceolatus venom has been purified by gel filtration on sephadex g-50 and ion exchange chromatography on cm-cellulose. analysis by fplc on mono-q column of the purified phospholipase a2 indicated that it is a mixture of several isoenzymes. the two major isoforms consist of a single polypeptide chain with mol. wts of 14,500 and 15,000, which slightly differ in their isoelectric point (4.9 and 5.3) and amino acid composition. however, enzymatic and pharma ... | 1994 | 7801343 |
| natural resistance of opossum (didelphis marsupialis) to the mapanare (bothrops lanceolatus) snake venom. | the inactivation of local and general effects of the mapanare (bothrops lanceolatus) venom by opossum's (didelphis marsupialis) serum fractions was tested using an in vivo assay and an in vitro preincubation experiment. a serum fraction of the opossum serum has been obtained by immunochemical purification. it is only present in opossum's protective opossum serum fraction (f-0.1). | 1993 | 8186456 |
| early infusion of a purified monospecific f(ab')2 antivenom serum for bothrops lanceolatus bites in martinique. | | 1996 | 8598738 |
| clinical indicators of envenoming and serum levels of venom antigens in patients bitten by bothrops lanceolatus in martinique. research group on snake bites in martinique. | an enzyme-linked immunosorbent assay was developed to measure venom antigen levels in the serum of 40 patients bitten by bothrops lanceolatus. the grading system used for the severity of envenomation (grades 1 to 4, minor to major) was predominantly based on the presence of local signs. serum venom levels increased with the grade of severity (p < 0.001, by spearman's rank correlation test); they were 6 +/- 6 ng/ml (mean +/- sd) in clinically non-envenomed patients (grade 1, n = 3), 7.6 +/- 11.7 ... | 1997 | 9196765 |
| purification from bothrops lanceolatus (fer de lance) venom of a fibrino(geno)lytic enzyme with esterolytic activity. | bothrops lanceolatus venom has high caseinolytic, phospholipasic, esterolytic and hemorrhagic activities. in spite of having no coagulant effect on plasma, this venom contains a thrombin-like enzyme. using gel filtration and ion-exchange chromatographies, we have purified an esterolytic fraction (f-ii-1a) from this venom with a protein yield of 4% and a 58% recovery in enzyme activity. sds-page in the presence of beta-mercaptoethanol showed that the enzyme is a single chain polypeptide with a mw ... | 1998 | 9655635 |
| prognostic significance of clinical grading of patients envenomed by bothrops lanceolatus in martinique. members of the research group on snake bite in martinique. | the correlation between clinical grading of patients bitten by bothrops lanceolatus and the subsequent development of their envenoming was examined. severity of envenoming was graded using a 1-4 scale (minor to major). patients were classified into 2 groups according to the time elapsed between bite and treatment with a specific purified equine f(ab')2 antivenom. the late/no treatment group (n = 33) was characterized by a systemic thrombotic complication rate of 14/33 (42.4%) leading to 4 deaths ... | 1998 | 9861375 |
| neutralization, by a monospecific bothrops lanceolatus antivenom, of toxic activities induced by homologous and heterologous bothírops snake venoms. | a monospecific bothrops lanceolatus antivenom, currently used in martinique, was tested for its efficacy in the neutralization of several toxic and enzymatic activities of the venoms of b. lanceolatus, b. atrox and b. asper. when tested by the i.p. route in mice, b. lanceolatus venom had an ld50 of 12.8 microg/g. in addition, it induced local tissue damage (hemorrhage, edema and myotoxicity) and showed indirect hemolytic activity, but was devoid of coagulant effect on human plasma in vitro and o ... | 1999 | 10080358 |
| bothrops lanceolatus (fer de lance) venom induces oedema formation and increases vascular permeability in the mouse hind paw. | the ability of snake venoms to increase vascular permeability and to induce oedema through the release of pharmacologically active substances is well known. we have studied the oedema and vascular permeability induced by bothrops lanceolatus venom in male swiss white mice. paw oedema was induced by the subplantar injection of b. lanceolatus venom (125-1000 ng/paw) and was quantified as the increase in paw weight. changes in vascular permeability were assessed by measuring the amount of evans blu ... | 2000 | 10665802 |
| coagulant and anticoagulant activities of bothrops lanceolatus (fer de lance) venom. | bothrops lanceolatus venom contains caseinolytic, phospholipase, esterase and haemorrhagic activities. we have investigated the coagulant and anticoagulant actions of b. lanceolatus venom on human citrated plasma and on purified plasma components. although b. lanceolatus venom up to 50 microg/ml was unable to clot citrated plasma, at concentrations > or = 5 microg/ml the venom dose-dependently clotted purified human fibrinogen, indicating the presence of a thrombin-like enzyme. human plasma (fin ... | 2001 | 10978756 |
| pharmacological characterization of the rat paw edema induced by bothrops lanceolatus (fer de lance) venom. | the inflammatory response induced by bothrops lanceolatus venom (blv) in the rat hind-paw was studied measuring paw edema. non-heated blv (75microg/paw) caused a marked paw edema accompanied by intense haemorrhage whereas heated venom (97 degrees c, 30s; 12.5-100microg/paw) produced a dose- and time-dependent non-haemorrhagic edema. the response with heated blv was maximal within 15min disappearing over 24h. heated venom was then routinely used at the dose of 75microg/paw. the prostacyclin analo ... | 2001 | 11137542 |
| neuromuscular action of bothrops lanceolatus (fer de lance) venom and a caseinolytic fraction. | a protein capable of inducing neuromuscular blockade in avian preparations and of depolarizing mouse diaphragm muscle was isolated from bothrops lanceolatus venom using gel filtration and ion-exchange chromatography. the purified protein was a single chain polypeptide with an estimated molecular mass of 27.5 kda by sds-page and had caseinolytic activity (13.3 units/mg), but no phospholipase a(2). b.lanceolatus venom (50 micro g/ml) and the caseinolytic protein (20 micro g/ml) produced contractur ... | 2002 | 12220713 |
| multiple cerebral infarctions following a snakebite by bothrops caribbaeus. | bothrops caribbaeus, a species of the bothrops complex, is found only in the island of saint lucia, west indies. snakebite from this pitviper is very rare. we report the case of a healthy 32-year-old saint lucian man who developed multiple cerebral infarctions following envenoming by this snake. this patient developed signs and symptoms very similar to those observed in patients envenomed by bothrops lanceolatus, a snake found only in martinique, the neighbor island of saint lucia. this clinical ... | 2002 | 12408668 |
| pharmacological and histopathological characterization of bothrops lanceolatus (fer de lance) venom-induced edema. | bothrops venoms cause local edema, pain, hemorrhage and necrosis. in this study, we investigated the ability of bothrops lanceolatus venom to cause edema in rat hind paws and examined the mediators involved. | 2004 | 15241562 |
| purification and characterization of a hemorrhagic metalloproteinase from bothrops lanceolatus (fer-de-lance) snake venom. | bothrops snake venoms contain metalloproteinases that contribute to the local effects seen after envenoming. in this work, a hemorrhagic metalloproteinase (blah1) was purified from the venom of the snake bothrops lanceolatus by a combination of gel filtration, affinity (metal chelating) and hydrophobic interaction chromatographies. the hemorrhagin was homogeneous by sds-page and had a molecular mass of 28 kda that was unaltered by treatment with beta-mercaptoethanol. blah1 gave a single band in ... | 2005 | 15733562 |
| [severe hypofibrinegenemia after rattlesnake envenomation in france]. | a 22-year-old man, who maintains illegally numerous exotic snakes at home (suburbs of paris), was bitten by one of his bresilian rattlesnakes, the lance-headed viper bothrops moojeni, with grade iii envenomation. the fibrinogen was less than 0,5 g/l, the prothombin time was 22%, the activated partial thromboplastin time was 94 seconds. the authors discuss the biological and clinical management of this defibrination, due to defibrinogenating proteases (thrombin-like enzymes), present in bothrops ... | 2005 | 15771982 |
| occipital infarction revealed by quadranopsia following snakebite by bothrops lanceolatus. | we report a case of snakebite in which envenomation was manifested through impairment of the visual field. the patient, a 46-year-old man, was bitten on the right thumb by bothrops lanceolatus. treatment with a specific equine antivenom (bothrofav) was administered one hour after the bite. with the exception of fang marks, the results of a clinical examination, particularly the neurologic component, were normal. the day after the bite, the patient developed an inferior left lateral homonymous qu ... | 2005 | 16172485 |
| thrombotic stroke following snake bites by the "fer-de-lance"bothrops lanceolatus in martinique despite antivenom treatment: a report of three recent cases. | the severity of envenoming from bothrops lanceolatus is determined by the development of cerebral, myocardial or pulmonary infarctions, and occasionnaly by serious local envenoming. introduction of specific antivenom has resulted in a dramatic improvement in the prognosis of this envenoming. against this background, we report 3 recent cases of patients bitten by b. lanceolatus who developed cerebral infarctions despite early administration of antivenom. | 2006 | 16750232 |
| fatal diffuse thrombotic microangiopathy after a bite by the "fer-de-lance" pit viper (bothrops lanceolatus) of martinique. | in martinique, a man bitten two days earlier by a pit viper (bothrops lanceolatus) was hospitalized with impaired consciousness and tetraplegia. investigations confirmed cerebral and myocardial infarctions. resolving thrombocytopenia was associated with virtually normal blood prothrombin time/activated partial thromboplastin time but increasing hyperfibrinogenemia. despite specific antivenom treatment, he developed fatal left ventricular failure six days after the bite. at autopsy, multiple cere ... | 2008 | 18541759 |
| snake venomics of the lesser antillean pit vipers bothrops caribbaeus and bothrops lanceolatus: correlation with toxicological activities and immunoreactivity of a heterologous antivenom. | the venom proteomes of the snakes bothrops caribbaeus and bothrops lanceolatus, endemic to the lesser antillean islands of saint lucia and martinique, respectively, were characterized by reverse-phase hplc fractionation, followed by analysis of each chromatographic fraction by sds-page, n-terminal sequencing, maldi-tof mass fingerprinting, and collision-induced dissociation tandem mass spectrometry of tryptic peptides. the venoms contain proteins belonging to seven ( b. caribbaeus) and five ( b. ... | 2008 | 18785768 |
| hyperbaric oxygen therapy after bothrops lanceolatus snake bites in martinique: a brief report. | every year 10 to 20 cases of snake bites are reported on the caribbean island of martinique. the only snake involved, bothrops lanceolatus, is endemic on the island, and its bite may lead to systemic multifocal thrombotic complications in the'absence of the monospecific antivenom. between january 1988 and january 2009, more than 250 snake bites have been reported, and five patients were treated with hyperbaric oxygen (hbo2) therapy for local complications. the patients were male, bitten on the l ... | 2010 | 21226390 |
| osteopontin, a chemotactic protein with cytokine-like properties, is up-regulated in muscle injury caused by bothrops lanceolatus (fer-de-lance) snake venom. | osteopontin (opn) is a chemotactic, adhesive protein whose receptors include some integrins and matrix proteins known to have role in inflammatory and repair processes. we examined the time course of opn expression at acute and chronic stages after intramuscular injection of bothrops lanceolatus venom in rats. additionally, we examined the expression of cd68 (a marker for phagocytic macrophages) and the myogenic factors, myod and myogenin. there was a biphasic upregulation of opn (6-48-áh and 3- ... | 2011 | 21839764 |
| bothrops lanceolatus bites: guidelines for severity assessment and emergent management. | approximately 20-30 declared snakebite cases occurin martinique each year. bothrops lanceolatus, a member of the crotalidae family, is considered to be the only involved snake. b. lanceolatus, commonly named "fer-de-lance", is endemic and only found on this caribbean island. envenomation local features include the presence of fang marks, swelling, pain, bleeding from punctures, and ecchymosis. severe envenomation is associated with multiple systemic thromboses appearing within 48 h of the bite a ... | 2010 | 22069552 |
| proteomic profiling of snake venom metalloproteinases (svmps): insights into venom induced pathology. | bothrops sp. snakebites account for the majority of envenomations in south and central america. bothrops jararaca accidents are characterized by edema, hemorrhage and necrosis, mainly attributed to the action of hemorrhagic snake venom metalloproteinases (svmps). interestingly, accidents involving bothrops lanceolatus (fer-de-lance) have a prothrombotic profile with necrosis and hemorrhage rarely reported. here we describe biochemical and proteomic approaches to compare the venom composition of ... | 2009 | 19539639 |
| inhibition of crotalidae venom hemorrhagic activities by didelphis marsupialis liver spheroids culture supernatants. | the main aim of this work was the development of a primary hepatocyte culture from didelphis marsupialis, to determine the possible use of culture medium supernatants as a source of inhibitors of the bothrops lanceolatus venom hemorrhagic activity. the cellular culture was carried out from isolated hepatocytes by the double perfusion technique, and digestion of the liver with collagenase and culturing the hepatocytes in a liquid media under continuous agitation at 37 degrees c in 5% co2. the hem ... | 2001 | 11405280 |
| antivenom activity of opossum (didelphis marsupialis) serum fraction. | we have found an opossum serum fraction of approximately 97,000 mol. wt to be highly proficient in inactivating the haemorrhagic and proteolytic fractions of bothrops lanceolatus venom. this antivenom substance, isolated from opossum serum or a synthetic peptide based on the aforementioned protein, would probably be useful in the medical management of bothrops accidents. | 1995 | 7778133 |
| bothrops lanceolatus (fer de lance) venom stimulates leukocyte migration into the peritoneal cavity of mice. | the ability of bothrops lanceolatus venom to induce neutrophil migration into the peritoneal cavity of mice was investigated. intraperitoneal injection of venom caused dose- and time-dependent neutrophil migration, which peaked with 750 ng of venom/cavity 4h after venom injection. the neutrophil migration was significantly reduced by pretreatment with dexamethasone (0.5 mg/kg, s.c.), an indirect inhibitor of phospholipase a(2) (pla(2)), and aa861 (0.01 mg/kg, s.c.), a 5-lipoxygenase inhibitor, b ... | 2003 | 12467667 |
| análisis clínico y epidemiológico de los accidentes por mordeduras de serpientes del género bothrops en venezuela [a clinical and epidemiological analysis of accidental bites by snakes of the genus bothrops in venezuela]. | clinical register of 60 patients bitten by bothrops snake who assisted at leopoldo manrique hospital and the institute of tropical medicine (hlm-imt) in caracas during 1996-1997 were analysed. the accident was more frequent in males (45/75%). in 32 cases (53.3%) the snake was classified and 26 were bothrops lanceolatus, 4 bothrops venezuelensis and 2 bothrops atrox. anatomic regions more frequent bitten were superior members (40/66.6%): hands (36/60%), forearm (2/3.3%), elbow (1/1.6%) and arm (1 ... | 2009 | 11107900 |
| elisa assays for the detection of bothrops lanceolatus venom in envenomed patient plasmas. | a double antibody sandwich enzyme linked immunosorbant assay (elisa) was carried out to detect bothrops ianceolatus venom in plasma from envenomed patients at various time intervals (0, 6, 12, 18 and 24 hrs). the test could detect bothrops lanceolatus levels up to 12 ng/ml of envenomed patient plasmas. elaboration of an easy, fast and species-diagnostic based on this elisa technique useful to physicians is discussed. | 2004 | 11845439 |
| haemorrhagic activity of neotropical rattlesnake (crotalus vegrandis klauber, 1941) venom. | in this work we have demonstrated for the first time in any venezuelan crotalus, haemorrhagic activities that are present in the neotropical uracoan rattlesnake (crotalus vegrandis) venom. this venom has been little studied, perhaps because the snake is restricted to a small habitat located in the dry savannahs of northeastern venezuela. in our experiments crotalus vegrandis venom caused a very evident haemorrhagic area consisting of approximately 2/3 diameter of the area caused by a positive co ... | 1998 | 9851507 |
| antivenom activity of opossum (didelphys marsupialis) serum fractions against uracoan rattlesnake (crotalus vegrandis klauber, 1941) venom. | in this work, we have found strong evidence for the presence of an opossum serum which is highly proficient in inactivating the neurotoxic fractions of uracoan rattlesnake (crotalus vegrandis) venom. analyses of strained electrophoretic patterns of sds gels run in non-reducing conditions revealed a small group of antivenom proteins in 0.1 m deae cellulose fraction that was not found in 0.05 m, 0.02 m, 0.25 m and 0.3 m nacl ionic strength. neutralizing activities to mapanare (bothrops lanceolatus ... | 2003 | 8993125 |
| [pulmonary embolism and disseminated intravascular coagulation after being bitten by a bothrops lanceolatus snake. apropos of a case]. | the authors report the case of a bothrops lanceolatus snake bite complicated by severe pulmonary embolism a few hours after admission. this thromboembolic complication developed despite heparin therapy and was followed by disseminated intravascular coagulation (dic). vascular thrombosis and pulmonary embolism are rare after bothrops lanceolatus snake bite as patients are usually hypocoagulable due to dic. in this case, the thromboembolism was probably caused by the procoagulant effect of the thr ... | 1989 | 2514645 |
| [specific serotherapy of envenomization by "bothrops lanceolatus". 1st therapeutic trials of monovalent serum prepared by institut pasteur]. | | 1970 | 5422390 |
| the ex vivo eye irritation test (eveit) model as a mean of improving venom ophthalmia understanding. | snakes belonging to the genus naja (elapid family), also known as "spitting cobras", can spit venom towards the eyes of the predator as a defensive strategy, causing painful and potentially blinding ocular envenoming. venom ophthalmia is characterized by pain, hyperemia, blepharitis, blepharospasm and corneal erosions. elapid venom ophthalmia is not well documented and no specific treatment exists. furthermore, accidental ejection of venom by non-spitting vipers, as bothrops, also occurs. the ex ... | 2018 | 29890230 |
| enzymatic and pro-inflammatory activities of bothrops lanceolatus venom: relevance for envenomation. | bothrops lanceolatus, commonly named 'fer-de-lance', is an endemic snake of the french caribbean island of martinique. envenomations by b. lanceolatus present clinical aspects characterized by systemic thrombotic syndrome and important local inflammation, involving edema and pain but limited hemorrhage. to investigate mechanisms of venom-induced inflammation, b. lanceolatus venom was characterized, its cross-reactivity with bothropic antivenom explored, its cytotoxicity on human keratinocytes an ... | 2017 | 28783135 |
| cellulitis and bacteremia after a snake bite despite antibiotic preemptive treatment: a diagnosis challenge. | | 2020 | 31935467 |
| infectious complications following snakebite by bothrops lanceolatus in martinique: a case series. | infections secondary to snakebite occur in a number of patients and are potentially life-threatening. bothrops lanceolatus bites in martinique average 30 cases per year and may result in severe thrombotic and infectious complications. we aimed to investigate the infectious complications related to b. lanceolatus bite. a retrospective single-center observational study over 7 years (2011-2018) was carried out, including all patients admitted to the hospital because of b. lanceolatus bite. one hund ... | 2020 | 31628740 |
| third-generation antivenomics analysis of the preclinical efficacy of bothrofav® antivenom towards bothrops lanceolatus venom. | bothrops lanceolatus inflicts severe envenomings in the lesser caribbean island of martinique. bothrofav®, a monospecific antivenom against b. lanceolatus venom, has proven highly effective at the preclinical and clinical levels. here, we report a detailed third-generation antivenomics quantitative analysis of bothrofav®. with the exception of poorly-immunogenic peptides, bothrofav® immunocaptured all the major protein components. these results, along with previous preclinical and clinical obser ... | 2019 | 32831345 |
| oral microbiota of the snake bothrops lanceolatus in martinique. | in martinique, bothrops lanceolatus snakebite, although relatively uncommon (~30 cases/year), may result in serious complications such as systemic thrombosis and local infections. infections have been hypothesized to be related to bacteria present in the snake's oral cavity. in this investigation, we isolated, identified, and studied the susceptibility to beta-lactams of bacteria sampled from the oral cavity of twenty-six b. lanceolatus specimens collected from various areas in martinique. micro ... | 2018 | 30261677 |
| case report: bothrops lanceolatus snakebite surgical management-relevance of fasciotomy. | bothrops lanceolatus is an endemic crotalidae species in martinique, where approximately 30 cases of envenoming are managed yearly. envenoming characteristics from bothrops species include local tissue damage, systemic bleeding, and hemodynamic alterations. we hereby report a case of severe envenomation following b. lanceolatus snakebite to the right calf. severe local manifestations developed progressively up to the lower limb despite adequate antivenom therapy. systemic manifestations of venom ... | 2018 | 30226147 |
| venom from bothrops lanceolatus, a snake species native to martinique, potently activates the complement system. | bothrops lanceolatus snake venom causes systemic thrombotic syndrome but also local inflammation involving extensive oedema, pain, and haemorrhage. systemic thrombotic syndrome may lead to fatal pulmonary embolism and myocardial and cerebral infarction. here, we investigated the ability of b. lanceolatus venom to activate the complement system (c) in order to improve the understanding of venom-induced local inflammation. data presented show that b. lanceolatus venom is able to activate all c-pat ... | 2018 | 30116749 |
| preclinical evaluation of the neutralizing ability of a monospecific antivenom for the treatment of envenomings by bothrops lanceolatus in martinique. | bothrops lanceolatus is an endemic viperid species in the lesser caribbean island of martinique. envenomings by this species are characterized by local and systemic effects, among which the development of thrombosis in various organs is the most severe complication. an experimental toxicological characterization of this venom was performed using in vivo mouse tests and various in vitro assays. the venom induced lethal, local and systemic hemorrhagic, edema-forming, myotoxic, thrombocytopenic, pr ... | 2018 | 29654867 |
| snake bites by bothrops lanceolatus in martinique. | bothrops lanceolatus, a member of the viperidae family and crotalinae subfamily, is the only snake causing envenomation in martinique, which is the only place it exists in the world. any bite by this snake may result in severe thrombotic complications including cerebral, pulmonary, and myocardial infarction and affecting the patient's vital or functional prognosis. the exact composition of the venom as well as the mechanisms of toxicity involved are still debated today. the first specific antive ... | 2018 | 29616641 |
| bothrops lanceolatus envenomation in a patient with arteriovenous fistula for hemodialysis access. | | 2016 | 26919642 |