| nucleotidase and dnase activities in brazilian snake venoms. | among the myriad of enzymes present in animal venoms, nucleotidases and nucleases are poorly investigated. herein, we studied such enzymes in 28 crude venoms of animals found in brazil. higher levels of atpase, 5'-nucleotidase, adpase, phosphodiesterase and dnase activities were observed in snake venoms belonging to bothrops, crotalus and lachesis genera than to micrurus genus. the venom of bothrops brazili snake showed the highest nucleotidase and dnase activities, whereas that of micrurus fron ... | 2008 | 17904425 |
| myotoxic phospholipases a(2) isolated from bothrops brazili snake venom and synthetic peptides derived from their c-terminal region: cytotoxic effect on microorganism and tumor cells. | this paper reports the purification and biochemical/pharmacological characterization of two myotoxic phospholipases a(2) (pla(2)s) from bothrops brazili venom, a native snake from brazil. both myotoxins (mtx-i and ii) were purified by a single chromatographic step on a cm-sepharose ion-exchange column up to a high purity level, showing m(r) approximately 14,000 for the monomer and 28,000da for the dimer. the n-terminal and internal peptide amino acid sequences showed similarity with other myotox ... | 2008 | 18602430 |
| structural and functional characterization of brazilitoxins ii and iii (bbtx-ii and -iii), two myotoxins from the venom of bothrops brazili snake. | we report the purification and biochemical/pharmacological characterization of two myotoxic pla(2) (bbtx-ii k49 pla(2) homologue and bbtx-iii pla(2)) from bothrops brazili venom. both were purified by a single chromatographic step on reverse phase hplc, showing m(r) approximately 14 kda for both myotoxins, showing high content of hydrophobic and basic amino acids as well as 14 half-cysteine residues. the bbtx-ii k49 pla(2) homologue and bbtx-iii pla(2), had a sequence of 121 amino acid residues. ... | 2009 | 19539640 |
| antigenic cross-reactivity and immunogenicity of bothrops venoms from snakes of the amazon region. | snakebites are still a critical public health problem in developing countries or isolated areas. in brazil, the north region has a high distribution coefficient worsened by the significant number of eventually unreported cases, due to difficulties in access to health services, to the natural geographic barriers and the vast territory. in the rio negro area, the species bothrops atrox, bothrops brazili, lachesis muta muta and bothriopsis taeniata are thought to be the major species responsible fo ... | 2010 | 20036275 |
| bbmp-1, a new metalloproteinase isolated from bothrops brazili snake venom with in vitro antiplasmodial properties. | this study describes the biochemical and functional characterization of a new metalloproteinase named bbmp-1, isolated from bothrops brazili venom. bbmp-1 was homogeneous on sds-page, presented molecular mass of 22,933da and pi 6.4. the primary structure was partially elucidated with high identity with others metalloproteinases from viperidae venoms. the enzymatic activity on azocasein was evaluated in different experimental conditions (ph, temperature). a significant reduction in enzyme activit ... | 2015 | 26363289 |
| inhibition of the myotoxicity induced by bothrops jararacussu venom and isolated phospholipases a2 by specific camelid single-domain antibody fragments. | antivenoms, produced using animal hyperimmune plasma, remains the standard therapy for snakebites. although effective against systemic damages, conventional antivenoms have limited efficacy against local tissue damage. additionally, the hypersensitivity reactions, often elicited by antivenoms, the high costs for animal maintenance, the difficulty of producing homogeneous lots, and the instability of biological products instigate the search for innovative products for antivenom therapy. in this s ... | 2016 | 27028872 |
| [experimental efficacy of igy antibodies produced in eggs against the venom of the peruvian snake bothrops atrox]. | to develop an immunization protocol in order to produce avian igy immunoglobulins against bothrops atrox peruvian snake venom and to evaluate its neutralizing capacity. | 2012 | 22510909 |
| serine proteinases from bothrops snake venom activates pi3k/akt mediated angiogenesis. | the discovery of rapid acting and powerful angiogenic proteins are of significant interest in the treatment of various human disorders associated with insufficient angiogenesis such as ischemia, menorrhagia and delayed wound healing. snake venoms consist of a mixture of bioactive proteins and polypeptides and are rich sources of pharmacologically important molecules. serine proteinases are one of the abundant proteins present in bothrops snake venoms and possess multiple biological functions inc ... | 2016 | 27816537 |
| bbrzsp-32, the first serine protease isolated from bothrops brazili venom: purification and characterization. | snake venom toxins are related not only in detention, death and the promotion of initial digestion of prey but also due to their different biochemical, structural and pharmacological effects they can result in new drugs. among these toxins snake venom serine proteases (svsps) should be highlighted because they are responsible for inducing changes in physiological functions such as blood coagulation, fibrinolysis, and platelet aggregation. this article presents the first serine protease (sp) isol ... | 2016 | 26827743 |
| genotoxic effect of bothrops snake venoms and isolated toxins on human lymphocyte dna. | in the present study, micronucleus with cytokinesis blocking and comet assays were used to evaluate the genotoxic potential of bothrops jararacussu, bothrops atrox, bothrops moojeni, bothrops alternatus (rhinocerophis alternatus) and bothrops brazili snake venoms, and also of some isolated toxins (mjtx-i, bthtx-i and ii myotoxins, bjussump-ii metalloprotease, and batxlaao l-amino acid oxidase) on human lymphocytes. significant dna damages were observed, indicating genotoxic potential after expos ... | 2013 | 23333649 |
| crystallization and preliminary x-ray diffraction analysis of three myotoxic phospholipases a2 from bothrops brazili venom. | two myotoxic and noncatalytic lys49-phospholipases a(2) (braziliantoxin-ii and mt-ii) and a myotoxic and catalytic phospholipase a(2) (braziliantoxin-iii) from the venom of the amazonian snake bothrops brazili were crystallized. the crystals diffracted to resolutions in the range 2.56-2.05 å and belonged to space groups p3(1)21 (braziliantoxin-ii), p6(5)22 (braziliantoxin-iii) and p2(1) (mt-ii). the structures were solved by molecular-replacement techniques. both of the lys49-phospholipases a(2) ... | 2012 | 22869126 |
| crystallographic portrayal of different conformational states of a lys49 phospholipase a₂ homologue: insights into structural determinants for myotoxicity and dimeric configuration. | catalytically inactive phospholipase a(2) (pla(2)) homologues play key roles in the pathogenesis induced by snake envenomation, causing extensive tissue damage via a mechanism still unknown. although, the amino acid residues directly involved in catalysis are conserved, the substitution of asp49 by arg/lys/gln or ser prevents the binding of the essential calcium ion and hence these proteins are incapable of hydrolyzing phospholipids. in this work, the crystal structure of a lys49-pla(2) homologu ... | 2012 | 22584077 |
| a new bothrops from brazil, bothrops brazili, sp. nov. | | 1953 | 13193513 |
| epidemiological studies of snake bite in french guiana. | the incidence of snake bite and the presence of venom antibody in previous snake bite victims was investigated in french guiana. the incidence proved to be highest (600/100,000) in inhabitants of bush regions and lowest (45/100,000) in urban areas. of 43 sera tested for specific venom antibody 22 (51%) were positive, and most of these individuals suffered severe or moderate poisoning. the main species involved, as assessed by detection of venom antibody by elisa, were lachesis muta, bothrops bra ... | 1987 | 3662671 |
| [study on the venoms of the principal venomous snakes from french guiana and the neutralization]. | we studied some biochemical, toxic and immunological characteristics of the venoms of bothrops atrox, bothrops brazili and lachesis muta, viperidae responsible for most of the bites of venomous snakes in french guiana. chromatographic (hplc) and electrophoretical profiles (sds-page), lethal, hemorrhagic, defibrinogenating, coagulant, thrombin like, proteolytic, fibrino(geno)lytic and phospholipase activities were studied. in addition, the neutralization of some toxic activities conferred by four ... | 2008 | 18956820 |
| venomics and antivenomics of the poorly studied brazil's lancehead, bothrops brazili (hoge, 1954), from the brazilian state of pará. | the brazil's lancehead, bothrops brazili, is a poorly studied pit viper distributed in lowlands of the equatorial rainforests of southern colombia, northeastern peru, eastern ecuador, southern and southeastern venezuela, guyana, suriname, french guiana, brazil, and northern bolivia. few studies have been reported on toxins isolated from venom of ecuadorian and brazilian b. brazili. the aim of the present study was to elucidate the qualitative and quantitative protein composition of b. brazili ve ... | 2020 | 32362928 |
| severe snakebite envenomation in french guiana: when antivenom is not available. | in french guiana, bothrops atrox, bothrops brazili, bothrops bilineatus, lachesis muta and micrurus sp are responsible for most cases of snakebite envenomation. the clinical features in patients suffering from envenomations by viperid snakes involve local tissue damage and systemic manifestations, such as hemorrhage, coagulopathies and hemodynamic instability. we report a severe case of envenomation in a patient bitten by a large unidentified pit viper in french guiana. due to lack of antivenom, ... | 2018 | 29621529 |
| anti-platelet aggregation activity of two novel acidic asp49-phospholipases a2 from bothrops brazili snake venom. | phospholipases a2 (pla2s) are important enzymes present in snake venoms and are related to a wide spectrum of pharmacological effects, however the toxic potential and therapeutic effects of acidic isoforms have not been fully explored and understood. due to this, the present study describes the isolation and biochemical characterization of two new acidic asp49-pla2s from bothrops brazili snake venom, named braziliase-i and braziliase-ii. the venom was fractionated in three chromatographic steps: ... | 2018 | 28951306 |
| proteomic and toxinological characterization of peruvian pitviper bothrops brazili ("jergón shushupe"), venom. | bothrops brazili is a pitviper from amazonian region, responsible for many accidents in peru. despite its relevance, its venom has not been extensively characterized. in the present work, bothrops brazili venom (bbv) components were analyzed by rp-hplc, sds-page and maldi-tof/tof. approximately 37 proteins were identified, belonging to 7 families. snake venom metalloproteinases (svmps) were the most abundant proteins of the venom (33.05%), followed by snake venom serine proteinases (svsps, 26.11 ... | 2020 | 32479836 |
| structural bases for a complete myotoxic mechanism: crystal structures of two non-catalytic phospholipases a2-like from bothrops brazili venom. | bothrops brazili is a snake found in the forests of the amazonian region whose commercial therapeutic anti-bothropic serum has low efficacy for local myotoxic effects, resulting in an important public health problem in this area. catalytically inactive phospholipases a2-like (lys49-pla2s) are among the main components from bothrops genus venoms and are capable of causing drastic myonecrosis. several studies have shown that the c-terminal region of these toxins, which includes a variable combinat ... | 2013 | 24145104 |