| diet and carbohydrate digestion in the yellow-spotted longicorn beetle psacothea hilaris. | larval and adult psacothea hilaris feed on mulberry wood and leaves, respectively. high levels of endogenous activity against the major dietary carbohydrates, cellulose, hemicellulose, starch and soluble sugars were secreted in the gut of larvae and adults. activity against pectin was also high and multiple polygalacturonase (ec 3.2.1.15) components were secreted in the gut of larvae. one glycanase component, beta-eg1, which was primarily an endo-beta-1,4-glucanase (ec 3.2.1.4) and another, beta ... | 1997 | 12770476 |
| change in significance of feeding during larval development in the yellow-spotted longicorn beetle, psacothea hilaris. | larvae of the west-japan type yellow-spotted longicorn beetle, psacothea hilaris (coleoptera: cerambycidae), show a long-day type photoperiodic response at 25 degrees c; under long-day conditions, larvae pupate after the fourth or fifth instar, while under short-day conditions, they undergo a few nonstationary supernumerary molts and eventually enter diapause. in the present study, the effect of food on the development and photoperiodic response of the larvae was examined with special reference ... | 2003 | 14511830 |
| antifungal properties and mode of action of psacotheasin, a novel knottin-type peptide derived from psacothea hilaris. | psacotheasin is a 34-mer knottin-type peptide that is derived from psacothea hilaris larvae. in this study, the antifungal activity and mechanism(s) by which psacotheasin affects human fungal pathogens were investigated. psacotheasin shows remarkable antifungal properties without hemolytic activity against human erythrocytes. to understand the antifungal mechanism(s) of psacotheasin in candida albicans, flow cytometric analysis with dibac(4)(3) and pi was conducted. the results showed that psaco ... | 2010 | 20735987 |
| isolation and characterization of psacotheasin, a novel knottin-type antimicrobial peptide, from psacothea hilaris. | we report the isolation and characterization of a novel knottin-type antimicrobial peptide from the yellow-spotted long-horned beetle psacothea hilaris. a cdna encoding a 56-mer knottin-type propeptide was identified and its predicted molecular mass and pi was 5.92 kda and 8.28, respectively. a 34-mer mature peptide was also selected and named herein as psacotheasin. the antimicrobial activity of chemically synthesized psacotheasin against human bacterial pathogens was subsequently investigated. ... | 2010 | 20467242 |
| photoperiodic response of larvae of the yellow-spotted longicorn beetle psacothea hilaris after removal of the stemmata. | the role of the stemmata in photoperiodism has been examined in holometabolic insects, but the only reliable results in coleoptera have been obtained in leptocarabus kumagaii (carabidae), the larvae of which do not respond to photoperiod without stemmata. in the present study, photoperiodism was examined in another coleopteran, psacothea hilaris (pascoe) (cerambycidae), after surgical removal of the stemmata. larvae reared under short-day conditions and transferred to long-day conditions on day ... | 2010 | 20230824 |
| complete mitochondrial genome sequence of the yellow-spotted long-horned beetle psacothea hilaris (coleoptera: cerambycidae) and phylogenetic analysis among coleopteran insects. | we have determined the complete mitochondrial genome of the yellow-spotted long horned beetle, psacothea hilaris (coleoptera: cerambycidae), an endangered insect species in korea. the 15,856-bp long p. hilaris mitogenome harbors gene content typical of the animal mitogenome and a gene arrangement identical to the most common type found in insect mitogenomes. as with all other sequenced coleopteran species, the 5-bp long tagta motif was also detected in the intergenic space sequence located betwe ... | 2009 | 19390824 |
| relationship between rapid cold-hardening and cold acclimation in the eggs of the yellow-spotted longicorn beetle, psacothea hilaris. | rapid cold-hardening (rch) and cold acclimation (acc) were examined in eggs of the yellow-spotted longicorn beetle, psacothea hilaris (pascoe) (coleoptera: cerambycidae). when eggs incubated at 25 degrees c were transferred directly to conditions of -22 degrees c for 2h, less than 30% survived, whereas exposure to 0 degrees c for 4h prior to transfer to -22 degrees c increased survival to nearly 60%. the rapidly enhanced cold tolerance (rch) was transient and lost rapidly after 1h at 25 degrees ... | 2007 | 17628587 |
| molecular cloning and developmental expression of the gene encoding juvenile hormone esterase in the yellow-spotted longicorn beetle, psacothea hilaris. | juvenile hormone (jh) plays a key role in the regulation of growth, development, diapause and reproduction in insects. the regulation of jh titers in the insect body is therefore crucial throughout postembryonic development. one of the major pathways of jh metabolism is degradation by a highly selective enzyme, juvenile hormone esterase (jhe). we obtained a full-length cdna encoding jhe in psacothea hilaris (phjhe). the complete phjhe cdna sequence is comprised of 1989 bp with an open reading fr ... | 2007 | 17456444 |
| molecular cloning, expression, and enzymatic activity of a novel endogenous cellulase from the mulberry longicorn beetle, apriona germari. | a novel endogenous beta-1,4-endoglucanase (ag-egase iii) gene belonging to the glycoside hydrolase family (ghf) 5 was cloned from the mulberry longicorn beetle, apriona germari. the ag-egase iii gene spans 1061 bp and consists of a single exon coding for 325 amino acid residues. the ag-egase iii showed 89% protein sequence identity to another beetle, psacothea hilaris, cellulase belonging to ghf 5. the ag-egase iii has the potential proton donor and nucleophile amino acids conserved in ghf 5 and ... | 2006 | 16945565 |
| endocrine changes associated with the starvation-induced premature metamorphosis in the yellow-spotted longicorn beetle, psacothea hilaris. | under 25 degrees c and a long day photoperiod, about half of the fourth instar psacothea hilaris larvae molt to the fifth instar on day 13 and pupate about 18 days later; the rest pupate without a further larval molt with a mean fourth instar period of 24 days. however, starvation of fourth instar larvae exceeding a threshold weight induces premature pupation, resulting in the formation of small but morphologically normal adults. to clarify the endocrine basis for this premature pupation, hemoly ... | 2005 | 16024020 |
| feeding glucose or sucrose, but not trehalose, suppresses the starvation-induced premature pupation in the yellow-spotted longicorn beetle, psacothea hilaris. | under 25 degrees c and a long-day photoperiod, starvation induces premature pupation in 4th instar psacothea hilaris larvae exceeding a threshold weight of 180 mg, resulting in the formation of small but morphologically normal adults. to investigate possible mechanisms underlying this phenomenon, we first measured the hemolymph trehalose and glucose levels of starved larvae. when larvae were starved after 4 days of feeding (attaining the threshold weight), glucose levels decreased 4-fold within ... | 2005 | 15963528 |
| endocrine changes associated with metamorphosis and diapause induction in the yellow-spotted longicorn beetle, psacothea hilaris. | at 25 degrees c and under a long-day photoperiod, all 5th instar psacothea hilaris larvae pupate at the next molt. under a short-day photoperiod, in contrast, they undergo one or two additional larval molts and enter diapause; the 7th instar larvae enter diapause without further molt. the changes in hemolymph juvenile hormone (jh iii) titers, jh esterase activity, and ecdysteroid titers in pupation-destined, pre-diapause, and diapause-destined larvae were examined. jh titers of the 5th instar pu ... | 2004 | 15607510 |
| evidence for the presence of a threshold weight for entering diapause in the yellow-spotted longicorn beetle, psacothea hilaris. | under long-day conditions larvae of psacothea hilaris (coleoptera: cerambycidae) pupate after the 4th or 5th instar, while under short-day conditions they undergo 2-4 nonstationary supernumerary molts and eventually enter diapause. to explore the possibility of a threshold weight for entering diapause, p. hilaris larvae were deprived of food on days 0 (day of ecdysis), 4 or 8 of the 4th, 5th and 6th instars under short-day conditions. within the first 40 days of starvation, 60% of the larvae sta ... | 2004 | 15081822 |
| purification, characterization, cdna cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, psacothea hilaris. | a cellulase (endo-beta-1,4-glucanase, ec 3.2.1.4) was purified from the gut of larvae of the yellow-spotted longicorn beetle psacothea hilaris by acetone precipitation and elution from gels after native page and sds/page with activity staining. the purified protein formed a single band, and the molecular mass was estimated to be 47 kda. the purified cellulase degraded carboxymethylcellulose (cmc), insoluble cello-oligosaccharide (average degree of polymerization 34) and soluble cello-oligosaccha ... | 2003 | 12899703 |