| isolation of a species-specific mitochondrial dna sequence for identification of tilletia indica, the karnal bunt of wheat fungus. | mitochondrial dna (mtdna) from five isolates of tilletia indica was isolated and digested with several restriction enzymes. a 2.3-kb ecori fragment was chosen, cloned, and shown to hybridize with total dna restricted with ecori from t. indica and not from a morphologically similar smut fungus, tilletia barclayana. the clone was partially sequenced, and primers were designed and tested under high-stringency conditions in pcr assays. the primer pair ti1/ti4 amplified a 2.3-kb fragment from total d ... | 1996 | 8572716 |
| detection of fungal infectous agent of wheat grains in store-pits of markazi province, iran. | wheat is an economic and important crop that provides approximately 20% of food calorie in the world. it is first crop in iran and cultivated in the most areas of this country. store-pit fungi make undesirable changes in quality and appearance of wheat grains. even, some fungi produce different mycotoxins which are toxic to human and livestock's that use wheat grains as source of food. in this study, several samples were randomly collected from each of five store-pits located in different areas ... | 2004 | 15756837 |
| identification of new wheat common bunt pathotypes (tilletia laevis kuhn.). | common bunt (cb) is the most important wheat disease after the rusts and the development of bunt-resistant cultivars is one of the best methods to control the disease. to identify new sources of resistance genes, identification of different pathotypes of this disease is essential. for this purpose, 449 isolates of cb collected from rainfed wheat fields in different regions of kermanshah province, west of iran were evaluated. out of which, 417 isolates were identified as tilletia laevis. accordin ... | 2006 | 17390864 |