| replication of reptilian paramyxovirus in avian host systems. | the reptilian paramyxovirus gov replicated in chicken embryo fibroblasts, in embryonated chicken eggs and in explanted chorio-allantoic membrane with titres of up to 10(8.2) tcid50/ml at 28 degrees c. the virus did not multiply above 30 degrees c. gov re-isolated from the avian host systems was identified by immunofluorescence and by immunogold-electron microscopy. | 1999 | 10085774 |
| adaptation of reptilian paramyxovirus to mammalian cells (vero cells). | the reptilian paramyxovirus gov was successfully adapted to vero cells in 80 passages at 30 degrees c. the virus replicated with ha titres of 1:64 and 10(7.5) tcid50/ml in the heterologous host cells forming syncytia, giant cells and cytoplasmic inclusion bodies. after 80 passages gov was identified by immunofluorescence, by immune electron microscopy and by pcr. sequencing of rt-pcr products of gov after 80 passages did not reveal changes in the nucleotide sequence of the partial l-gene of gov. | 2000 | 10763377 |
| complete genome sequence of fer-de-lance virus reveals a novel gene in reptilian paramyxoviruses. | the complete rna genome sequence of the archetype reptilian paramyxovirus, fer-de-lance virus (fdlv), has been determined. the genome is 15,378 nucleotides in length and consists of seven nonoverlapping genes in the order 3' n-u-p-m-f-hn-l 5', coding for the nucleocapsid, unknown, phospho-, matrix, fusion, hemagglutinin-neuraminidase, and large polymerase proteins, respectively. the gene junctions contain highly conserved transcription start and stop signal sequences and tri-nucleotide intergeni ... | 2004 | 14747569 |
| sequence motifs and prokaryotic expression of the reptilian paramyxovirus fusion protein. | fourteen reptilian paramyxovirus isolates were chosen to represent the known extent of genetic diversity among this novel group of viruses. selected regions of the fusion (f) gene were sequenced, analyzed and compared. the f gene of all isolates contained conserved motifs homologous to those described for other members of the family paramyxoviridae including: signal peptide, transmembrane domain, furin cleavage site, fusion peptide, n-linked glycosylation sites, and two heptad repeats, the secon ... | 2006 | 16328138 |
| a unique novel reptilian paramyxovirus, four atadenovirus types and a reovirus identified in a concurrent infection of a corn snake (pantherophis guttatus) collection in germany. | in 2009, 26 clinical samples (organs and oral/cloacal swabs) from a total of 24 corn snakes (pantherophis guttatus) from a single owner were sent to our laboratory to be tested for the presence of viruses. paramyxoviruses (pmv), adenoviruses (adv) and reoviruses were detected by rt-pcr, pcr and virus isolation methods. three snakes were infected with all three viruses at the same time, while two other snakes had a double infection (pmv and reo, adv and reo) and nine other snakes had a single inf ... | 2011 | 21316873 |