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determination and differentiation of triacylglycerol molecular species in antarctic and non-antarctic yeasts by atmospheric pressure-chemical ionization-mass spectrometry.yeast, particularly saccharomyces cerevisiae, has long served as a model eukaryotic system for studies on the regulation of lipid metabolism. we developed a high performance liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry method for the detailed analysis of triacylglycerols (tags) in 14 species of yeast consisting of seven antarctic yeasts (grown at 15°c and 5°c) and seven non-antarctic yeasts (grown at 25°c and 15°c), the latter including 3 strains of s. cerevis ...201323831436
the diversity, extracellular enzymatic activities and photoprotective compounds of yeasts isolated in antarctica.the diversity of yeasts collected from different sites in antarctica (admiralty bay, king george island and port foster bay and deception island) and their ability to produce extracellular enzymes and mycosporines were studied. samples were collected during the austral summer season, between november 2006 and january 2007, from the rhizosphere of deschampsia antarctica, ornithogenic (penguin guano) soil, soil, marine and lake sediments, marine water and freshwater from lakes. a total of 89 isola ...201124031709
extracellular enzymes produced by microorganisms isolated from maritime antarctica.antarctic environments can sustain a great diversity of well-adapted microorganisms known as psychrophiles or psychrotrophs. the potential of these microorganisms as a resource of enzymes able to maintain their activity and stability at low temperature for technological applications has stimulated interest in exploration and isolation of microbes from this extreme environment. enzymes produced by these organisms have a considerable potential for technological applications because they are known ...201222806048
cryptococcus species (tremellales) from glacial biomes in the southern (patagonia) and northern (svalbard) hemispheres.cryptococcus species (basidiomycota) were isolated as the predominant yeast from glacial biomes of both patagonia (argentina) and the svalbard archipelago (norway). for a selected group of cryptococcus belonging to tremellales, assimilative profile, production of extracellular hydrolytic enzymes and ribosomal dna internal transcribed spacer and large subunit (d1/d2) sequences were analysed. cryptococcus victoriae, which was originally described from antarctica, was the most frequently found spec ...201222861821
taxonomic assessment and enzymes production by yeasts isolated from marine and terrestrial antarctic samples.the aim of the present study was to investigate the taxonomic identity of yeasts isolated from the antarctic continent and to evaluate their ability to produce enzymes (lipase, protease and xylanase) at low and moderate temperatures. a total of 97 yeast strains were recovered from marine and terrestrial samples collected in the antarctica. the highest amount of yeast strains was obtained from marine sediments, followed by lichens, ornithogenic soils, sea stars, salpa sp., algae, sea urchin, sea ...201324114281
polyphenolic substrates and dyes degradation by yeasts from 25 de mayo/king george island (antarctica).antarctica offers a range of extreme climatic conditions, such as low temperatures, high solar radiation and low nutrient availability, and constitutes one of the harshest environments on earth. despite that, it has been successfully colonized by ’cold-loving’ fungi, which play a key role in decomposition cycles in cold ecosystems. however, knowledge about the ecological role of yeasts in nutrient or organic matter recycling/mineralization remains highly fragmentary. the aim of this work was to ...201324298603
electrospray ionization-tandem mass spectrometry analysis of phospholipid molecular species from antarctic and non-antarctic yeasts.high performance liquid chromatography-electrospray ionization tandem mass spectrometry was applied to the comprehensive analysis of phospholipids from seven antarctic and seven non-antarctic yeasts. identification of specific fatty acyl moieties to the sn-1 and sn-2 positions of phosphatidylethanolamine (pe), phosphatidylcholine (pc), phosphatidylserine (ps) and phosphatidylinositol (pi) were determined by relative abundance of fragment ions associated with formation of carboxylate anions and l ...201425019517
[resistance of various yeast ecological groups to prolonged storage in dry state].resistance of 14 yeast species belonging to different ecological groups to extensive storage in a dried state was investigated. pedobiotic yeasts isolated mainly from the soils of humid areas (cryptococcus podzolicus, cr. terricola, and lipomyces starkeyi) were the least resistant. the yeasts associated with the nectar of entomophilous plants (metschnikowia reukaufii and candida bombi) also exhibited low resistance to drying. complete death of these species occurred during the first month of sto ...201526263698
lipidomic analysis of psychrophilic yeasts cultivated at different temperatures.analysis of polar lipids from eight psychrophilic yeasts (cryptococcus victoriae, cystofilobasidium capitatum, holtermaniella wattica, mrakiella aquatica, m. cryoconiti, rhodotorula lignophila, kondoa malvinella and trichosporon aggtelekiense) grown at 4-28°c by hydrophilic interaction liquid chromatography/high resolution electrospray ionization tandem mass spectrometry determined 17 classes of lipids and identified dozens of molecular species of phospholipids including their regioisomers. most ...201627422372
yeasts from macroalgae and lichens that inhabit the south shetland islands, antarctica.antarctic terrestrial ecosystems are largely dominated by lichens, while shallow coastal environments are mainly covered by macroalgae. the aim of this study was to isolate and to evaluate the diversity of yeasts in different species of macroalgae and lichens collected in south shetland islands, antarctica. a total of 405 yeasts were recovered (205 from macroalgae and 200 from lichens). the yeast community from macroalgae was most diversity than the yeast community from lichen. the dominance ind ...201627518570
assessment of endophytic yeast diversity in rice leaves by a culture-independent approach.endophytic microorganisms inhabit internal plant tissues in the host plant without causing any symptoms or negative effects. although the diversity of endophytes has been evaluated by both culture-dependent and culture-independent methods, less information is available on yeast communities. therefore, in this study a culture-independent method was used to examine endophytic yeasts associated with rice leaves based on the large subunit of ribosomal dna using a semi-nested pcr technique. sequence ...201526122889
Cryptococcus foliicola sp. nov. and Cryptococcus taibaiensis sp. nov., novel basidiomycetous yeast species from plant leaves.A molecular taxonomic investigation performed on basidiomycetous yeast strains isolated from plant leaves collected in two areas of China revealed two novel species, Cryptococcus foliicola sp. nov. (type strain HS 23.3(T) = AS 2.2471(T)= CBS 9920(T)) and Cryptococcus taibaiensis sp. nov. (type strain ST 7.9(T) = AS 2.2444(T)= CBS 9919(T)), among the non ballistoconidium-forming strains producing cream-colored colonies. These new species differed markedly from closely related species in the inter ...201122139457
cryptococcus statzelliae sp. nov. and three novel strains of cryptococcus victoriae, yeasts isolated from antarctic soils.a morphological and physiological characterization of yeast strains cbs 8908, cbs 8915, cbs 8920, cbs 8925(t) and cbs 8926, isolated from antarctic soils, was performed. phylogenetic analyses of the sequences of the d1/d2 regions and the adjacent internal transcribed spacer (its) regions of the large-subunit rdna of these strains placed them into the tremellales clade of the hymenomycetes. the sequence data identified strains cbs 8908, cbs 8915 and cbs 8920 as belonging to the species cryptococc ...200212508901
cryptococcus tephrensis, sp.nov., and cryptococcus heimaeyensis, sp.nov.; new anamorphic basidiomycetous yeast species from iceland.two new species from iceland are described on the basis of physiological profiles and sequence data from the d2 region of lsu rdna: cryptococcus tephrensis (type ice99-itom y5, atcc mya-1765, cbs 8935, genbank af317208) and cryptococcus heimaeyensis (type ice99-itom y8, atcc mya-1759, cbs 8933, genbank af370717). the two new species are identifiable from sequence data and can be distinguished from their closest relative, cryptococcus victoriae, by their higher maximum temperatures for growth, fa ...200212109887
lichensphere: a protected natural microhabitat of the non-lichenised fungal communities living in extreme environments of antarctica.we surveyed the diversity, distribution and ecology of non-lichenised fungal communities associated with the antarctic lichens usnea antarctica and usnea aurantiaco-atra across antarctica. the phylogenetic study of the 438 fungi isolates identified 74 taxa from 21 genera of ascomycota, basidiomycota and zygomycota. the most abundant taxa were pseudogymnoascus sp., thelebolus sp., antarctomyces psychrotrophicus and cryptococcus victoriae, which are considered endemic and/or highly adapted to anta ...201526400492
correlation of maple sap composition with bacterial and fungal communities determined by multiplex automated ribosomal intergenic spacer analysis (marisa).during collection, maple sap is contaminated by bacteria and fungi that subsequently colonize the tubing system. the bacterial microbiota has been more characterized than the fungal microbiota, but the impact of both components on maple sap quality remains unclear. this study focused on identifying bacterial and fungal members of maple sap and correlating microbiota composition with maple sap properties. a multiplex automated ribosomal intergenic spacer analysis (marisa) method was developed to ...201121569942
polyphasic taxonomy of a novel yeast isolated from antarctic environment; description of cryptococcus victoriae sp. nov.in 1992 some samples of mosses, lichens and soils were collected from botany bay, southern victoria land (77 degrees 01' s 162 degrees 32' e) and, as a result of a routine screening programme some yeasts were isolated. one of them, designated as strain g5, showed marked differences when compared to other antarctic yeasts. according to morphological and physiological characteristics, we were able to identify the strain g5 as a yeast belonging to the genus cryptococcus. some characteristics of thi ...199910188283
application of issr-pcr for rapid strain typing of debaryomyces hansenii isolated from dry-cured iberian ham.yeast populations of dry-cured iberian ham isolated from seven industries in the province of badajoz were characterized by issr-pcr using the (cag)4 primer and pcr-rflp of the its1-5.8s rrna-its2 fragment, and identified by dna sequencing. a total of 242 isolates were analyzed, indicating the primary species present was debaryomyces hansenii at 80.9% of the isolates followed by candida zeylanoides at 10.3% of the isolates. the remainders of isolates were identified as yamadazyma triangularis, sp ...201424929738
yeasts from an oligotrophic lake in patagonia (argentina): diversity, distribution and synthesis of photoprotective compounds and extracellular enzymes.nahuel huapi (nh) lake is an oligotrophic temperate lake of glacial origin with high transparency, surrounded by well-developed forests and located at san carlos de bariloche, nahuel huapi national park, in patagonia, argentina. in this lake, we characterized yeast distribution and diversity along a south-to-north transect and established a relationship between the ability to produce photoprotective compounds (ppcs) (carotenoid pigments and mycosporines) and the occurrence of yeast at different ...201121223324
analysis of fungal flora in indoor dust by ribosomal dna sequence analysis, quantitative pcr, and culture.in recent years increasing attention has been given to the potential health effects of fungal exposure in indoor environments. we used large-scale sequencing of the fungal internal transcribed spacer region (its) of nuclear ribosomal dna to describe the mycoflora of two office buildings over the four seasons. dna sequencing was complemented by cultivation, ergosterol determination, and quantitative pcr analyses. sequences of 1,339 clones were clustered into 394 nonredundant fungal operational ta ...200817981947
three new combinations from the cryptococcus laurentii complex: cryptococcus aureus, cryptococcus carnescens and cryptococcus peneaus.fifteen strains of the cryptococcus laurentii complex were reclassified based on sequence analyses of 18s rdna, the d1/d2 region of the 26s rdna and the internal transcribed spacer regions, as well as physiological and biochemical properties. the strains were divided into phylogenetic groups i and ii. the type strain of c. laurentii (cbs 139t=atcc 18803t=jcm 9066t=mucl 30398t=nrrl y-2536t) was in phylogenetic group i. phylogenetic group ii, which was phylogenetically distant from phylogenetic gr ...200312892148
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