evolution of temperature optimum in thermotogaceae and the prediction of trait values of uncultured organisms.quantitative characterization of the mode and rate of phenotypic evolution is rarely applied to prokaryotes. here, we present an analysis of temperature optimum (t (opt)) evolution in the thermophilic family thermotogaceae, which has a large number of cultured representatives. we use log-rate-interval analysis to show that t (opt) evolution in thermotogaceae is consistent with a brownian motion (bm) evolutionary model. the properties of the bm model are used to a establish confidence intervals o ...201121638056
atopostipes suicloacale gen. nov., sp. nov., isolated from an underground swine manure storage pit.phenotypic and molecular genetic studies were performed on an unknown facultative anaerobic, catalase-negative, non-spore-forming, rod-shaped bacterium isolated from a pig manure storage pit. the unknown bacterium was nutritionally fastidious with growth enhanced by the addition of rumen fluid and was phenotypically initially identified as an eubacterium species. comparative 16s rrna gene sequencing studies, however, revealed that the unknown bacterium was phylogenetically distant from eubacteri ...200416701518
endocarditis due to a new rod-shaped neisseria sp.we report the first case of pacemaker endocarditis due to a new rod-shaped neisseria sp. isolated from blood culture. on the basis of rrs sequencing, the isolate was found to be most closely related to an uncultured organism from human subgingival plaque and was identified as neisseria sp. group ak105. a cure was achieved after a combination of surgical and antibiotic treatment. oral flora-induced pacemaker endocarditis is a rare condition that reinforces the need for good oral hygiene as an imp ...200515695696
phylogenetic analysis of some sporomusa sub-branch members isolated from human clinical specimens: description of megasphaera micronuciformis sp. nov.two unknown, gram-negative, catalase-negative and strictly anaerobic cocci were isolated from two independent human samples (strains aip 49.01 and aip 412.00t). comparative 16s rrna gene sequencing demonstrated that these two organisms displayed 99.8% sequence identity and that they are members of the sporomusa sub-branch of the low-g + c gram-positive bacteria. the most closely related 16s rdna sequences were from megasphaera sp. oral clone bu057 (99.8%) and from isolates of megasphaera cerevis ...200312710625
microbiology of enhanced biological phosphorus removal in aerated-anoxic orbal processes.the traditional process for enhanced biological phosphorus removal (ebpr) in wastewater treatment involves an anaerobic zone followed by an aerobic zone. although there is no strict anaerobic zone in aerated-anoxic orbal processes, phosphorus removal in excess of that required for cell growth does occur. the microbial ecology of polyphosphate-accumulating organisms (pao) in two full-scale orbal wastewater treatment plants was investigated using flow cytometry to physically separate pao from non- ...200212469947
dna analysis of intracellular campylobacter-like organisms associated with the porcine proliferative enteropathies: novel organism proposed.intracellular campylobacter-like organisms are a consistent feature of the porcine proliferative enteropathies. the relationship between these organisms and known campylobacter sp. previously associated with the disease was studied using restriction enzyme analysis and dna-dna blot hybridization techniques. bglii restriction enzyme fragment patterns of dna of the campylobacter-like organisms were fundamentally different from those of c. mucosalis, c. hyointestnalis, c. jejuni, and c. coli. crude ...19902210329
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