| fucogalactan isolated from sarcodon aspratus elicits release of tumor necrosis factor-alpha and nitric oxide from murine macrophages. | eight species of mushrooms were evaluated for mitogenic activity by the tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (mtt) method using spleen cells of c3h/hen female mice. the hot water-soluble (hws) fraction extracted from sarcodon aspratus showed the highest activity. the mitogen in sa. aspratus was isolated by sepharose 6b and deae-sepharose cl-6b column chromatography. preliminary structural analyses indicated that the mitogen was a fucogalactan. fucogalact ... | 2000 | 10647870 |
| ceramide constituents from five mushrooms. | five mushrooms, panellus serotinus, lyophyllum connatum, amanita pantherina, sarcodon aspratus and lepista nuda, have been investigated chemically. two new ceramides, (2s,3r,4e,8e)-n-hexadecanoyl-2-amino-9-methyl-4,8-octadecadiene-1,3-diol (1) and (2s,3r,4e,8e,9'z,12'z)-n-9',12'-octadecadienoyl-2-amino-9-methyl-4,8-octadecadiene-1,3-diol (2), have been isolated from panellus serotinus. compound 2 was also isolated from lyophyllum connatum. two new ceramides, (2s,2'r,3r,4e,8e)-n-2'-hydroxypentade ... | 2002 | 12036031 |
| ergosterol peroxide, an apoptosis-inducing component isolated from sarcodon aspratus (berk.) s. ito. | among the lipophilic extracts of seven traditional edible mushrooms, the acetone extract of sarcodon aspratus markedly inhibited the growth of hl60 human leukemia cells and induced apoptosis after 24 h incubation. the major active component was identified as ergosterol peroxide by nmr and esi-ms analysis. ergosterol peroxide completely inhibited growth and induced apoptosis of hl60 cells at a concentration of 25 microm. | 2005 | 15665489 |
| identification of bacteria isolated from diseased neungee mushroom, sarcodon aspratus. | as the first step in an investigation of the problem with quality deterioration seen in the neungee mushroom (sarcodon aspratus) due to bacterial overgrowth during its storage, an attempt to isolate bacterial strains was made using infected gills of sarcodon aspratus. five bacterial strains were isolated; one phototrophic cyanobacterial species and four heterotrophic gram negative rods. the four heterotrophic bacterial isolates (strains p, s, r, and mk1) were subjected to identification based on ... | 2007 | 17304616 |
| antitumor activity of sarcodon aspratus (berk.) s. ito and ganoderma lucidum (fr.) karst. | antitumor activity of sarcodon aspratus (berk.) s. ito and ganoderma lucidum (fr.) karst. was investigated. methanol and aqueous extracts of these japanese mushrooms were tested for antitumor activity against solid type of sarcoma 180 by intraperitoneal or oral administration. the aqueous extract was remarkably effective for inhibition of tumor growth, but the methanol extract was not. the fraction of molecular weight more than 10000 had a high inhibitory activity against the tumor growth, but t ... | 1989 | 2746451 |
| activation of macrophages by exopolysaccharide produced by mk1 bacterial strain isolated from neungee mushroom, sarcodon aspratus. | the mk1 strain, a novel bacterial isolate from soft-rotten tissue of the neungee mushroom, produces copious amounts of exopolysaccharide (eps) in a dextrose minimal medium. this study examined the molecular characteristics and immunomodulatory activity of mk1 eps. | 2010 | 21286384 |
| structure elucidation and immunological activity of a novel polysaccharide from the fruit bodies of an edible mushroom, sarcodon aspratus (berk.) s. ito. | a water-soluble polysaccharide with a molar weight of 4.3x10(5)da, termed as hbp was isolated from the fruit bodies of an edible mushroom, sarcodon aspratus (berk.) s. ito. composition, methylation analysis, fourier transform infrared spectroscopy, and nuclear magnetic resonance spectroscopy experiments were conducted to elucidate its structure. the results indicated that hbp is a glucan featuring a backbone of (1-->6)-linked-beta-d-glucopyranosyl residues, which occasionally branched at o-3 pos ... | 2010 | 20621666 |
| 5alpha,8alpha-epidioxy-22e-ergosta-6,9(11),22-trien-3beta-ol from an edible mushroom suppresses growth of hl60 leukemia and ht29 colon adenocarcinoma cells. | we purified a sterol with antitumor activity from the edible mushroom sarcodon aspratus (berk.) s. ito and identified it as 5alpha,8alpha-epidioxy-22e-ergosta-6,9(11),22-trien-3beta-ol (9,11-dehydroergosterol peroxide (9(11)-dhep)). purified 9(11)-dhep was a more effective inhibitor of hl60 leukemia cell growth and stronger apoptosis-inducer than 5alpha,8alpha-epidioxy-22e-ergosta-6,22-dien-3beta-ol (ergosterol peroxide (ep)) that we had previously identified as an apoptosis inducer. moreover, 9 ... | 2006 | 16595913 |