| visualization of nodulation gene activity on the early stages of rhizobium leguminosarum bv. viciae symbiosis. | a technique was optimized for the in situ detection of nodulation (nod) gene activity in rhizobium leguminosarum bv. viciae symbiosis with compatible plant hosts vicia tetrasperma (l.) schreb. and pisum sativum l. the transcription of nodabc-lacz fusion was visualized as beta-galactosidase (beta-gal) activity after reaction with the chromogenic substrate x-gal and subsequent light microscopy, while the background of the indigenous beta-gal activity of rhizobia and the host plant was eliminated b ... | 2005 | 16408851 |
| larvicidal activity of leguminous seeds and grains against aedes aegypti and culex pipiens pallens. | larvicidal activity of methanol extracts of 26 leguminous seeds and 20 grains against early 4th-stage larvae of aedes aegypti and culex pipiens pallens was examined. at 200 ppm of the extracts from cassia obtusifolia, cassia tora, and vicia tetrasperma, more than 90% mortality was obtained in larvae of ae. aegypti and cx. pipiens pallens. extract of c. tora gave 86.7 and 100% mortality in the larvae of ae. aegypti and cx. pipiens pallens at 40 ppm but 59.2 and 78.3% mortality against larvae of a ... | 2002 | 12322944 |
| vicia tetrasperma (four-seeded vetch) ingestion by a 3-year-old child. | we report an ingestion of vicia tetrasperma (four-seeded vetch), initially misidentified as crown vetch (coronilla varia). vicia tetrasperma is not listed in poisindex; little is known of its toxic effects and we found no published human case reports. the child suffered only minor gastrointestinal effects which lasted a few hours and had no residual effects upon 24- and 96-hour followups. | 1993 | 8249267 |
| purification and characterization of a new dimeric mannose/glucose-binding isolectin from vicia tetrasperma (l.) schreber. | a new mannose/glucose specific isolectin vtl-ii has been purified to electrophoretic homogeneity from the seeds of vicia tetrasperma (l.) schreber through successive steps of (i) lectin extraction, (ii) ammonium sulfate fractionation (30-50%), and (iii) affinity chromatography on a column of sephadex g-50 covalently coupled with d-mannose. the isolectin was found to be a dimeric protein of molecular weight 62 kda made up of apparently chemically identical subunits unlike the tetrameric isolectin ... | 2009 | 19090421 |
| visualization of symbiotic tissue in intact root nodules of vicia tetrasperma using gfp-marked rhizobium leguminosarum bv. viciae. | in rhizobial symbiosis with legume plant hosts, the symbiotic tissue in the root nodules of indeterminate type is localized to the basal part of the nodule where the symbiotic zones contain infected cells (ic) interspersed with uninfected cells (uc) that are devoid of rhizobia. although ic are easily distinguished in nodule sections using standard histochemical techniques, their observation in intact nodules is hampered by nodule tissue characteristics. tagging of rhizobium leguminosarum bv. vic ... | 2008 | 18500633 |
| determination of symbiotic nodule occupancy in the model vicia tetrasperma using a fluorescence scanner. | fluorescent tagging of nodule bacteria forming symbioses with legume host plants represents a tool for vital tracking of bacteria inside the symbiotic root nodules and monitoring changes in gene activity. the constitutive expression of heterologous fluorescent proteins, such as green fluorescent protein (gfp), also allows screening for nodule occupancy by a particular strain. imaging of the fluorescence signal on a macro-scale is associated with technical problems due to the robustness of nodule ... | 2011 | 21262794 |