Publications

TitleAbstractYear
Filter
PMID
Filter
axial vessel widening in arborescent monocots.dicotyledons have evolved a strategy to compensate for the increase in hydraulic resistance to water transport with height growth by widening xylem conduits downwards. in monocots, the accumulation of hydraulic resistance with height should be similar, but the absence of secondary growth represents a strong limitation for the maintenance of xylem hydraulic efficiency during ontogeny. the hydraulic architecture of monocots has been studied but it is unclear how monocots arrange their axial vascul ...201424488857
performance and characteristics of carcass and non-carcass components of lambs fed peach-palm by-product.the objective of this study was to evaluate the effects of supplying the by-product of peach-palm (bactris gasipaes) on performance and characteristics of carcass and non-carcass components of feedlot lambs. twenty santa ines lambs of 150 days average age and 22.4 ± 3.4 kg body weight were confined in individual pens. a completely randomized design was utilized with four experimental diets composed of: fresh peach-palm by-product enriched with urea, fresh peach-palm by-product + concentrate, sil ...201323712399
survival and ultrastructural features of peach palm (bactris gasipaes, kunth) somatic embryos submitted to cryopreservation through vitrification.bactris gasipaes (arecaceae), also known as peach palm, was domesticated by amazonian indians and is cultivated for its fruit and heart-of-palm, a vegetable grown in the tree's inner core. currently, the conservation of this species relies on in situ conditions and field gene banks. complementary conservation strategies, such as those based on in vitro techniques, are indicated in such cases. to establish an appropriate cryopreservation protocol, this study aimed to evaluate the ultrastructural ...201323636432
lipid-dissolved γ-carotene, β-carotene, and lycopene in globular chromoplasts of peach palm (bactris gasipaes kunth) fruits.high levels of β-carotene, lycopene, and the rare γ-carotene occur predominantly lipid-dissolved in the chromoplasts of peach palm fruits. first proof of their absorption from these fruits is reported. the structural diversity, the physical deposition state in planta, and the human bioavailability of carotenoids from the edible fruits of diverse orange and yellow-colored peach palm (bactris gasipaes kunth) varieties were investigated. hplc-pda-ms(n) revealed a broad range of carotenes, reaching ...201425023631
chemical characterization and antioxidant activity of amazonian (ecuador) caryodendron orinocense karst. and bactris gasipaes kunth seed oils.nowadays, data concerning the composition of caryodendron orinocense karst. (euphorbiaceae) and bactris gasipaes kunth (arecaceae) seed oils are lacking. in light of this fact, in this paper fatty acids and unsaponifiable fraction composition have been determined using gc-ms, hplc-dad (diode array detector), nmr approaches and possible future applications have been preliminary investigated through estimation of antioxidant activity, performed with dpph test. for c. orinocense linoleic acid (85.5 ...201425391685
silage or fresh by-product of peach palm as roughage in the feeding of lambs.the objective of this study was to evaluate intake and apparent digestibility of agro-industrial by-product of peach palm in diets for lambs. twenty castrated, crossbred santa ines lambs, with average age of 150 days and body weight of 22.4 ± 3.4 kg, were distributed in a completely randomized design with four experimental diets composed of the following: fresh by-product of peach palm enriched with urea + ammonia sulfate (fu); fresh peach palm by-product + concentrate (fp); silage of peach palm ...201525604074
carbohydrate composition of peach palm (bactris gasipaes kunth) by-products flours.the flours obtained from peach palm by-products are rich in dietary fiber (62-71%) and they can be used as food ingredients. the aim of this work was to investigate the carbohydrate composition of the flours processed from the residual parts (stem and median sheath) of a hearts-of-palm industry. the flours were fractionated, based on their solubility, whose monomeric compounds were determined. the fraction containing mostly cellulose (s5) was the most abundant (26-28%), followed by the sum of fr ...201525839811
optimization of ultrasonic-assisted extraction of total carotenoids from peach palm fruit (bactris gasipaes) by-products with sunflower oil using response surface methodology.the present study reports on the extraction of total carotenoids from peach palm fruit by-products with sunflower oil. response surface methodology (rsm) was used to investigate the effect of process variables on the ultrasound-assisted extraction (uae). three independent variables including ultrasonic intensity (764-1528, w/m(2)), temperature (25-45°c), and the extraction time (10-30 min). according to the results, the optimal uae condition was obtained with an ultrasonic intensity of 1528 w/m( ...201525911166
conservation implications of the mating system of the pampa hermosa landrace of peach palm analyzed with microsatellite markers.peach palm (bactris gasipaes) is cultivated by many indigenous and traditional communities from amazonia to central america for its edible fruits, and is currently important for its heart-of-palm. the objective of this study was to investigate the mating system of peach palm, as this is important for conservation and breeding. eight microsatellite loci were used to genotype 24 open-pollinated progenies from three populations of the pampa hermosa landrace maintained in a progeny trial for genetic ...201525983626
carotenoid deposition in plant and animal foods and its impact on bioavailability.over the past decades, an enormous body of literature dealing with the natural deposition of carotenoids in plant- and animal-based foods has accumulated. prominent examples are the large solid-crystalline aggregates in carrots and tomatoes or the lipid-dissolved forms in dairy products and egg yolk. latest research has identified lipid-dissolved forms in a rare number of plant foods, such as tangerine tomatoes and peach palm fruit (bactris gasipaes kunth). in addition, liquid-crystalline forms ...201726115350
carotenoid composition and antioxidant activity of the raw and boiled fruit mesocarp of six varieties of bactris gasipaes.total carotenoid content and composition of carotenoids of six varieties of bactris gasipaes were determined by spectrophotometry and hplc, with photodiode array detector. significant differences in total carotenoid content (1.1 to 22.3 mg/100g) were detected among these varieties. boiling the fruits for 30 minutes did not affect total carotenoid content, but did change the amount of some specific carotenoids, mainly by the production of z-isomers. peach palm varieties had the same carotenoids, ...201021090177
[leaf micrografic anatomy of the neotropical palm bactris gasipaes (arecaceae)].the economic importance of the palm bactris gasipaes is growing in the neotropoical region. we collected leaflets from plants under a chemical fertilization regime and a population of 5000 plants per hectare, in costa rica. the variety, diamantes 10, has an ascendency fom the upper amazon basin. we used harries hematoxiline, eocine and standard light microscopy techniques. the presence of raphids and buliform cells was confirmed for the abaxial surface of the leaflets and for the hypodermic tiss ...200819256456
[evaluation of consumer's acceptance of a peach palm snack (bactris gasipaes) and determination of its potential as a functional food].the aim of this study was to evaluate consumers' acceptance of a peach palm snack and to determine its potential as a functional food by chemical characterization. an assessment was conducted with 100 consumers to determine the acceptance of different snack formulations and the results were subjected to cluster analysis. this analysis revealed two groups. group 2 included people that consume snacks and peach palm frequently and showed the highest grades for the snack evaluated characteristics. a ...201526320306
assessment of nano cellulose from peach palm residue as potential food additive: part ii: preliminary studies.high consumption of dietary fibers in the diet is related to the reduction of the risk of non-transmitting of chronic diseases, prevention of the constipation etc. rich diets in dietary fibers promote beneficial effects for the metabolism. considering the above and recognizing the multifaceted advantages of nano materials, there have been many attempts in recent times to use the nano materials in the food sector including as food additive. however, whenever new product for human and animal consu ...201526344977
identification and quantification of carotenoids, by hplc-pda-ms/ms, from amazonian fruits.the major and minor carotenoids from six fruits, buriti (mauritia vinifera), mamey (mammea americana), marimari (geoffrola striata), peach palm (bactrys gasipaes), physalis (physalis angulata), and tucuma (astrocaryum aculeatum), all native to the amazonia region, were determined by high-performance liquid chromatography-photodiode array detector-mass spectrometry detector (hplc-pda-ms/ms), fulfilling the recommended criteria for identification. a total of 60 different carotenoids were separated ...200717530774
cryopreservation of peach palm zygotic embryos.cryopreservation is a safe and cost-effective option for long-term germplasm conservation of non-orthodox seed species, such as peach palm (bactris gasipaes). the objective of the present study was to establish a cryopreservation protocol for peach palm zygotic embryos based on the encapsulation-dehydration technique. after excision, zygotic embryos were encapsulated with 3 percent sodium alginate plus 2 m glycerol and 0.4 m sucrose, and pre-treated or not with 1 m sucrose during 24 h, followed ...201317369958
somatic embryogenesis in peach-palm (bactris gasipaes) using different explant sources.peach palm (bactris gasipaes kunth) is a member of the family arecaceae and is a multipurpose but underutilized species. nowadays, fruit production for subsistence and local markets, and heart-of-palm production for local, national, and international markets are the most important uses of this plant. conventional breeding programs in peach palm are long-term efforts due to the prolonged generation time, large plant size, difficulties with controlled pollination and other factors. although it is ...201626619867
an integrated hypothesis on the domestication of bactris gasipaes.peach palm (bactris gasipaes kunth) has had a central place in the livelihoods of people in the americas since pre-columbian times, notably for its edible fruits and multi-purpose wood. the botanical taxon includes both domesticated and wild varieties. domesticated var gasipaes is believed to derive from one or more of the three wild types of var. chichagui identified today, although the exact dynamics and location of the domestication are still uncertain. drawing on a combination of molecular a ...201526658881
intake, digestibility and performance of lambs fed diets containing peach palm meal.the aim of this study was to evaluate the intake and apparent digestibility of nutrients, performance, and plasma glucose concentration of ram lambs fed diets containing peach palm meal substituting maize (0, 10, 40, 60, and 85 % dry matter (dm)). thirty santa inês rams with an average initial body weight of 21.6 ± 0.87 kg were distributed in a completely randomized design with five diets and six replicates. the substitution of the maize for the peach palm meal affected (p < 0.05) the intakes of ...201626781510
amazonian native palm fruits as sources of antioxidant bioactive compounds.the amazon region has many sources of fruits, especially native ones not yet explored, but which have some potential for use, as is the case with certain palms. the objective of this study was to evaluate the content of bioactive compounds and total antioxidant capacities of fruits from native palms from the brazilian amazon. the fruits of five palm species (bacaba, buriti, inajá, pupunha, and tucumã) were evaluated for levels of ascorbic acid, anthocyanins, yellow flavonoids, total carotenoids, ...201526783846
nitrogen metabolism in lambs fed diets containing peach palm meal.the objective of this study was to evaluate the nitrogen metabolism of lambs fed diets containing peach palm meal substituting maize (0, 10, 40, 60, and 85 % dm). thirty santa inês uncastrated lambs with an average initial body weight of 21.6 ± 0.87 kg were distributed in a completely randomized design with five diets and six replicates. the nitrogen (n) intake (g/day, %bw) and the fecal n decreased linearly as the peach palm meal was added to the concentrate. the urinary n, however, responded q ...201627307278
genetic analysis identifies the region of origin of smuggled peach palm seeds.seeds of a plant, supposedly a palm tree known popularly as peach palm (bactris gasipaes), were seized by the federal police in the state of pará, brazil, without documentation of legal origin to authorize transportation and marketing in brazil. they were alleged to be from the western part of amazonas, brazil, near the frontier with peru and colombia, justifying the lack of documentation. the species was confirmed to be peach palm. to determine the likely place of origin, a genetic analysis was ...201728262385
centesimal composition and physical-chemistry analysis of the edible mushroom lentinus strigosus occurring in the brazilian amazon.the centesimal composition and the physical and chemical analyses of lentinus strigosus, an edible mushroom occurring in the brazilian amazon and produced in alternative substrates based on wood and agroindustrial residues, were evaluated. for this purpose, the c, n, ph, soluble solids, water activity, protein, lipids, total fiber, ash, carbohydrate, and energy levels were determined. the substrates were formulated from simarouba amara aubl. ("marupá"), ochroma piramidale cav. ex. lam. ("pau-de- ...201324141410
morphoanatomy of the flower of syagrus inajai (spruce) becc. (arecaceae- arecoideae- attaleinae), amazon.the occurrence of syagrus inajai (spruce) becc., popularly known as pupunha palm, among other names, has been registered in the guianas and in the north of brazil in areas of terra firme (non-flooding) and gallery forests. in order to characterize the inflorescence and further knowledge of this family, a morphoanatomical study was carried out of the palm s. inajai in a green area of the campus of the federal university of amazonas--ufam, manaus, amazonas. the inflorescences are branched to one o ...201324212708
tdz pulsing evaluation on the in vitro morphogenesis of peach palm.peach palm (bactris gasipaes kunth.) cropping is an excellent alternative to native species exploitation; nevertheless, the problems with seed germination and conventional propagation justify the use of in vitro culturing. aiming to asses tdz pulsing effect on b. gasipaes morphogenesis, explants obtained from unarmed microplants were maintained in two treatments, half of them in ms free medium (without growth regulator) and the other half in ms with tdz (0.36 μm). both groups were transferred to ...201324431497
[Proximal composition, lipid and cholesterol content of meat from pigs fed peach-palm meal (Bactris gasipaes Kunth) and synthetic lysine].Two experiments were conducted to evaluate the proximal composition, lipids and cholesterol content of meat from pigs fed diets with peach-palm meal (PPM), with or without addition of synthetic lysine (LYS). In experiment 1, 24 pigs were randomly allotted into six treatments with three levels of PPM (0.16 and 32%) and two levels of LYS (0 and 0.27%). In experiment II, 16 finishing pigs were fed with two levels of PPM (0 and 17.50%) and two levels of LYS (0 and 0.27%). At the end of each experime ...201122097296
effects of peach palm oil on performance, serum lipoproteins and haemostasis in broilers.1. an experiment was conducted to study the comparative effects of peach palm oil (ppo, bactris gasipaes h.b.k), crude palm oil (cpo, elaeis guinenesis), maize oil (mo) and beef tallow (bt) on serum total and lipoprotein cholesterol levels and haemostatic factors in broiler chickens. 2. four experimental diets were formulated to be isocaloric (14·2 mj ame(n)/kg) and isonitrogenous (230 g cp/kg). ppo was extracted from the whole dry fruit with hexane. each fat was added to the diet in an amount e ...201021161785
[bipolaris bicolor (mitra) shoemaker: species associated to folial spot in pupunha palm (bactris gasipaes kunth) in brazil.].one species of hiphomycetos group, belonging to the genus bipolaris shoemaker that was identified like bipolaris bicolor (mitra) shoemaker is recorded for the first time on pupunha palm (bactris gasipaes kunth) from brazil. the comparison with other close species reported like pathogenic folial spot in genus arecaceae is made. its morphological and cultural characteristics are described.199817655408
the consumption of red pupunha (bactris gasipaes kunth) increases hdl cholesterol and reduces weight gain of lactating and post-lactating wistar rats.the lactating and post-lactating periods are marked by large metabolic change. production of milk is 60% lipid dependent. we reported in a recent scientific meeting that red pupunha palm tree fruit increases hdl cholesterol in lactating rats. this study evaluated if consumption of red pupunha by adult female rats has a beneficial impact on the lipid metabolism of lacting and post-lacting adult rats.201325580386
development of a novel micro-assay for evaluation of peroxyl radical scavenger capacity: application to carotenoids and structure-activity relationship.a micro-assay was developed and validated, using a microplate reader in 96-well format, c(11)-bodipy(581/591) as fluorescent probe and aibn as roo() generator. the structure-activity relationship was established for 15 carotenoid standards, indicating that the opening of the β-ionone ring and the increase of chromophore extension in the carotenoid structure were the major factors leading to the increase of roo() scavenging capacity. the values for roo() scavenging capacity were calculated using ...201222953962
estimation of glycaemic index of peach palm (bactris gasipaes) cooked fruits and chips, and pitahaya (hylocereus spp.) pulp.the glycaemic index (gi) is a physiological measure of a food's potential to increase postprandial blood glucose, as compared to the effect produced by food taken as reference, such as glucose or white bread. currently researchers and consumers are interested in low gi foods, since their consumption is associated with better weight control and reduced risk of incidence of chronic diseases, like diabetes. in the present study, the gi value for peach palm cooked fruit, peach palm chips and pitahay ...201224617026
genetic divergence among populations and accessions of the spineless peach palm from pampa hermosa landrace used in the heart-of-palm agribusiness in brazil.although originally domesticated for its fruit, exploitation of the peach palm (bactris gasipaes kunth) in the production of gourmet heart-of-palm has also become an important activity, hence the need for improved material for large-scale production, on employing the pampa hermosa landrace as the seed source. in this study 11 microsatellite markers were used to evaluate genetic divergence among 96 elite plants representing four populations of spineless peach palm from the above cited source. gen ...201222888298
arabinogalactan proteins and the extracellular matrix surface network during peach palm somatic embryogenesis.somatic embryogenesis has been described in peach palm as a reliable method for its in vitro multiplication and conservation. in this study, we evaluated the possible role of arabinogalactan proteins (agps) during this morphogenetic pathway. the presence of yariv reagent, a synthesized chemical antibody that specifically binds agp molecules, affected somatic embryos and callus development rate, but no effect was observed on fresh weight increment. this substance also had profound effects on embr ...201222574975
pre-procambial cells are niches for pluripotent and totipotent stem-like cells for organogenesis and somatic embryogenesis in the peach palm: a histological study.the direct induction of adventitious buds and somatic embryos from explants is a morphogenetic process that is under the influence of exogenous plant growth regulators and its interactions with endogenous phytohormones. we performed an in vitro histological analysis in peach palm (bactris gasipaes kunth) shoot apexes and determined that the positioning of competent cells and their interaction with neighboring cells, under the influence of combinations of exogenously applied growth regulators (na ...201222534682
enzyme characterisation, isolation and cdna cloning of polyphenol oxidase in the hearts of palm of three commercially important species.heart of palm (palmito) is the edible part of the apical meristem of palms and is considered a gourmet vegetable. palmitos from the palms euterpe edulis (juçara) and euterpe oleracea (açaí) oxidise after harvesting, whereas almost no oxidation is observed in palmitos from bactris gasipaes (pupunha). previous investigations showed that oxidation in juçara and açaí was mainly attributable to polyphenol oxidase (ppo; ec 1.14.18.1) activity. in this study, we partially purified ppos from these three ...201121530289
a temporary immersion system improves in vitro regeneration of peach palm through secondary somatic embryogenesis.secondary somatic embryogenesis has been postulated to occur during induction of peach palm somatic embryogenesis. in the present study this morphogenetic pathway is described and a protocol for the establishment of cycling cultures using a temporary immersion system (tis) is presented.201121355009
ecological adaptation of wild peach palm, its in situ conservation and deforestation-mediated extinction in southern brazilian amazonia.the arc of fire across southern amazonia seasonally attracts worldwide attention as forests are cut and burned for agricultural expansion. these forests contain numerous wild relatives of native south american crops, such as peach palm.200919238213
somatic embryogenesis in peach palm using the thin cell layer technique: induction, morpho-histological aspects and aflp analysis of somaclonal variation.the thin cell layer (tcl) technique is based on the use of very small explants and has allowed enhanced in vitro morphogenesis in several plant species. the present study evaluated the tcl technique as a procedure for somatic embryo production and plantlet regeneration of peach palm.200717670751
genetic differentiation and trade among populations of peach palm ( bactris gasipaes kunth) in the peruvian amazon-implications for genetic resource management.peach palm ( bactris gasipaes kunth) is cultivated for fruit and 'heart of palm', and is an important component of agroforestry systems in the peruvian amazon. in this study, aflp was used to compare genetic diversity among domesticated populations along the paranapura and cuiparillo rivers, which are managed by indigenous and colonist farming communities, respectively. gene diversity was 0.2629 for the populations in indigenous communities and 0.2534 in colonist communities. genetic differentia ...200414985969
chemical composition of the fruit mesocarp of three peach palm (bactris gasipaes) populations grown in central amazonia, brazil.the percent composition, soluble and insoluble food fibers, oil fatty acids and minerals were determined in the mesocarp of fruits of three peach palm (bactris gasipaes kunth) populations grown in central amazonia, brazil. amino acids were also determined in one of the populations. the mean protein levels ranged from 1.8 to 2.7%, lipid levels ranged from 3.5 to 11.1%, the nitrogen free fraction ranged from 24.3 to 35%, food fiber ranged from 5.2% to 8.7%, and energy ranged from 179.1 to 207.4 kc ...200312701237
[fatty acids contained in 4 pejibaye palm species, bactris gasipaes (palmae)].cooked and uncooked samples from four pejibaye palm races were analyzed to determine the moisture content, ether extract and the content of six fatty acids (c16:0 to c18:3). there was an increase in moisture and a decrease in ether extract (p < 0.05) in the cooked samples in comparison with the uncooked ones. no significant differences were found in fatty acid content between cooked and uncooked samples, but there were differences (p < 0.05) among races concerning the content of four fatty acids ...20088728758
plant cells which aid in pollen digestion within a beetle's gut.the peach palm, bactris gasipaes h.b.k., in costa rica, possesses unusual trichomes on the inflorescence epidermal surface. certain cells of the trichome possess a thick, highly lignified cell wall and are consumed by the beetle cyclocephala amazona l. before it ingests pollen from the same inflorescence. chemical analyses show the trichome to possess no nutritive value. the thick-walled trichome cells pass intact through the beetle's digestive system, while ingested pollen is crushed. we sugges ...199028312721
[use of flour of pejibaye fruit (bactris gasipaes h.b.k.) in bread making].trial were conducted in costa rica in 1984 and 1985, to determine the possibility of substituting pejibaye (bactris gasipaes h.b.k.) meal for wheat flour in bread. utilization in three distinct mixtures was examined: 90:10, 85:15 and 80:20 percentage of wheat flour to percentage of pejibaye meal, respectively. the breads were made, and dough analyses were conducted at "molinos de costa rica, s.a.", the country's principal flour mill. chemical analyses were carried out at the university of costa ...19873454612
composition and nutritive value of pejibaye (bactris gasipaes) in animal feeds.nutritive assessment of pejibaye (bactris gasipaes) meals included proximal composition of the lipid and nitrogenous fractions. caloric values obtained as true metabolizable energy (tme) indicate that the pejibaye has a higher content of energy than corn and that it is not necessary to separate the seeds from the fruits in animal feeds; the level of indispensable aminoacids is considerably low, especially methionine, which is lower than in corn; thin layer chromatography shows that most of the f ...19846535181
[effect of supplementation with peach palm as source of vitamin a: study with rats].the effect of supplementation with peach palm (bactris gasipaes h.b.k.), as an alternative source of vit. a, in the regional diet of manaus, am, brazil, in which the pulp was cooked and transformed into flour, was studied. the biological trial involved rats which were depleted in zinc and vitamin a, followed by repletion using the regional diet (rd), rd plus peach palm and rd plus vitamin a. the parameters used to determine the utilization of vitamin a were the vitamin a concentrations in the li ...19969008923
[content and bioavailability of carotenoids from peach palm fruit (bactris gasipaes) as a source of vitamin a].four introductions of ripe peach palm fruit (bactris gasipaes) were analyzed for ether extract, total carotenoids and their biological conversion into vitamin a. also, edible portion in cooked fruit was estimated. ether extract content in raw fruit ranged from 8.2 to 12.9% dry basis (db), cooked between 5.7 to 12.4% db and nutrient retention after cooking was 69 to 100%. carotenoids content in raw and cooked pejibaye was similar, 4.8 to 29.6% db and 4.8 to 29.9% db, respectively, giving a nutrie ...19921341854
latin american food sources of carotenoids.latin america has a wide variety of carotenogenic foods, notable for the diversity and high levels of carotenoids. a part of this natural wealth has been analyzed. carrot, red palm oil and some cultivars of squash and pumpkin are sources of both beta-carotene and alpha-carotene. beta-carotene is the principal carotenoid of the palm fruits burití, tucumã and bocaiuva, other fruits such as loquat, marolo and west indian cherry, and sweet potato. buriti also has high amounts of alpha-carotene and g ...199910971848
[genetic diversity and kin relationships among wild and cultivated populations of the pejibaye palm (bactris gasipaes, palmae) using microsatellite markers].genetic diversity and kin relationships among wild and cultivated populations of the pejibaye palm (bactris gasipaes, palmae) using microsatellite markers. the genetic diversity of the peach palm (pejibaye, bactris gasipaes kunth) was evaluated using four nuclear dna microsatellites in an effort to elucidate the evolution and domestication of this crop. a total of 258 samples from seven wild populations and eleven races were analyzed. all loci were polymorphic and a total of 50 alleles were iden ...200818624239
a survey of pig production systems in the rain forest of the pacific coast colombia.a questionnaire-based survey was conducted in small-scale pig farms in the rain forest of the colombian pacific coast in order to study aspects of reproduction, nutrition and productivity. a total of 124 farmers was interviewed. they owned, on average, 13.6 pigs, including 2.3 sows, mainly of the zungo breed. pigs are reared in extensive systems and are allowed to wander freely in search of food. the sows produce, on average, 9.6 piglets/litter but, owing to poor sanitary conditions, 1.5 are bor ...200515934639
Displaying items 1 - 49 of 49