| presence of a vicia unijuga lectin-binding (vgu) glycoprotein with thomsen-friedenreich (t) activity and vgu glycoproteins in human primary hepatocellular carcinoma. | 1. twenty perchloric acid-soluble glycoprotein fractions (pasfs) were separated from carcinomatous and non-carcinomatous regions of the livers of 5 patients with primary hepatocellular carcinoma (hcc) and 5 patients with a complication of hcc and hepatocirrhosis (hc). 2. in autoradiography using 125i-labelled vicia unijuga lectin (vua) and 125i-labelled arachis hypogaea anti-t lectin as probes, 10 pasfs from carcinomatous regions of the livers of 10 patients gave 1 vgu glycoprotein with t activi ... | 1992 | 1330763 |
| [serological property of perchloric acid-soluble glycoprotein fractions of human meconium]. | perchloric acid-soluble fractions (pasfs) were separated, in yields of 87.0-151.9 mg per 1.0 g of wet meconium sample, from six samples of human meconium, and identified as glycoproteins having 41.2-74.1% carbohydrate by chemical composition analysis. six pasfs reacted with anti-a, -b, -lea and -leb sera, but did not react with anti-m and -n sera. these pasfs alos reacted with many lectins such as dba, uea-i, vicia graminea lectin (vga), vicia unijuga lectin (vua), con a, wga, rca-i, pha-e, sba, ... | 1993 | 8396178 |
| [extract from leaves of vicia unijuga for isolation of antigen from human blood]. | | 1976 | 1005394 |
| presence of vicia graminea or vicia unijuga lectin-binding (vgu) glycoproteins with and without thomsen-friedenreich (t) antigen in normal human seminal plasma. | vgu glycoprotein (vicia graminea lectin- or vicia unijuga lectin-binding glycoprotein) has been reported as oncofetal antigen, which is found in many kind of tumor tissues, amniotic fluid and fetal membranes. in autoradiography with an 125i-labeled vicia unijuga lectin (vua) probe and an 125i-labeled arachis hypogaea lectin (pna, anti-t lectin) probe, seminal plasma samples of eight healthy men gave 2-7 vgu glycoproteins without t antigen, 1-2 vgu glycoproteins with thomsen-friedenreich antigen ... | 2001 | 12889521 |
| biosynthesis of vicia graminea lectin- and vicia unijuga lectin-binding glycoproteins in human tumor and nontumor cells and an estimation of its epitope structure. | we investigated biosynthesis of vicia graminea lectin (vga)- and vicia unijuga lectin (vua)-binding (vgu) glycoproteins, which are human malignant tumor-associated antigens, in cultured human tumor and non-tumor cells by pulse-labeling experiments with [35s]-methionine, followed by immunoprecipitation using immobilized vua, sds-page and autofluorography. it was shown that vgu glycoproteins synthesized by tumor cells were 15-30 times greater than those of non-tumor cells. it was also shown that a ... | 2000 | 10757124 |
| presence of vicia graminea lectin- and vicia unijuga lectin-binding (vgu) glycoproteins in human fetal membranes and some of their biochemical properties. | we have previously reported that vicia graminea lectin (vga)- and vicia unijuga lectin (vua)-binding glycoproteins (vgu glycoproteins), malignant tumor-associated antigens, exist in human meconium and amniotic fluid. to examine the origin of vgu glycoprotein, their presence, some of their chemical and serological properties and their biosynthesis in the human fetal membrane, amnion and chorion laeve and accompanying membrane cells were examined. perchloric acid-soluble fractions were prepared fr ... | 1998 | 9693961 |
| investigation of vicia graminea lectin- and vicia unijuga lectin-binding glycoproteins as novel oncofetal antigens in cyst and ascitic fluids of human ovarian tumors in benign, dysplastic, and malignant stages. | perchloric acid-soluble fractions (pasfs) were obtained from cyst fluids of human benign ovarian mucinous cystadenoma, benign dermoid cyst, "borderline" mucinous cystadenoma, and malignant ovarian clear cell carcinoma, and from fluids of malignant embryonal carcinoma and malignant serous cystadenocarcinoma. pasfs from benign lesions did not react with arachis hypogaea lectin (pna), vicia graminea lectin (vga), or vicia unijuga lectin (vua). pasfs from "borderline" lesions reacted with pna but di ... | 1997 | 9232320 |
| presence of vicia graminea lectin- and vicia unijuga lectin-binding (vgu) glycoprotein in human amniotic fluid and some of its chemical and serological properties. | vicia graminea- and vicia unijuga-binding glycoprotein (vgu glycoprotein) has been reported as a malignant tumor-associated antigen, which is found in various kinds of malignant tumor-tissues and ascitic and cyst fluids of malignant tumor patients, but not found in 20 kinds of normal human tissues. the glycoprotein reacts with anti-n lectins of vicia-graminea (vga) and vicia unijuga (vua), but does not react with anti-blood group m and n sera. the existence of vgu glycoprotein in human amniotic ... | 1997 | 9202424 |
| [vicia graminea lectin or vicia unijuga lectin-binding (vgu) glycoproteins as new tumor-associated substances]. | the purification and serological and chemical properties of vicia graminea lectin (vga) and vicia unijuga lectin (vua) were described, and then the binding-specificity of anti-m and -n antibodies and both the lectins was discussed in this review. on the basis of the facts that vgu glycoproteins which react with either vga or vua but not with anti-m and -n antibodies and were not detected in human normal organ tissues and sera, were separated and identified as mucin-type glycoproteins with very h ... | 1993 | 8487160 |
| presence of vicia graminea lectin- or vicia unijuga lectin-binding (vgu) glycoproteins, vgu glycoproteins with thomsen-friedenreich (t) activity and t-active glycoproteins in human meconium. | perchloric acid-soluble fraction prepared from a mixture of meconiums of 22 newborn babies was subjected to a systematic affinity chromatography using vicia unijuga lectin-cellulofine column and arachis hypogaea lectin-agarose column and separated into four fractions, vgu glycoprotein, vgu glycoprotein with t activity, t-active glycoprotein and another glycoprotein fractions. hplc analysis showed that the first fraction contained three glycoproteins with molecular weight (mw) of about 19,400 kda ... | 1995 | 7780836 |
| inhibition of phytoagglutinin potency of korean vicia unijuga by human urine. | human urines are often examined for individual identification in medicolegal field, therefore, authors attempted to classify the urines by inhibition of agglutination potency of phytoagglutinin korean vicia unijuga which is known as a panphytoagglutinin to human red blood cells, and demonstrated that some individuals excrete urine which has ability to interfere with the agglutination potency of vicia extracts. the inhibiting substance appears to be either a protein or a glycoprotein, for it can ... | 1989 | 2770033 |
| purification and characterization of anti-n lectin from vicia unijuga leaves. | 1. an anti-n lectin was extracted from vicia unijuga leaves with phosphate-buffered saline (pbs). purification of the lectin was achieved, after pretreatment of the pbs extract by ammonium sulfate fractionation and absorption with human m erythrocytes, by using a combination of conventional chromatographic techniques with asialoglycophorin an-sepharose cl-4b affinity chromatography. purification steps were followed by increase of specific activity. 2. homogeneity of the purified lectin was demon ... | 1990 | 2328820 |
| biochemical characterization of glycoprotein components in human ovarian cyst fluids by lectins. | 1. perchloric acid-soluble glycoprotein fractions (pasfs) were separated, in yields of 180-610 mg per 100 ml of cyst fluid, from the cyst fluids of human ovarian cystadenomas (ocas) in benign and borderline and ovarian clear cell carcinoma (occ) in malignant, and then identified as glycoproteins with 19.1-69.5% carbohydrate by their chemical composition analyses. 2. these pasfs reacted with anti-a, -b, -lea and/or -leb sera, but did not react with anti-h, -m and -n sera. all of the pasfs reacted ... | 1990 | 2165928 |
| [separation of thomsen-friedenreich (t) antigen and n antigen precursor glycoproteins from perchloric acid-soluble fraction of cyst fluid of human ovarian clear cell carcinoma and their some chemical and serological properties]. | one m perchloric acid-soluble fraction separated from cyst fluid of a patient (blood group ab) with ovarian clear cell carcinoma in malignant was subjected to sephacryl s-200 gel filtration and fraction 1 which was eluted at void volume, reacted with both of the lectins of vicia unijuga and arachis hypogaea. this fraction was then separated into 6 fractions (deae-frs. 1-6) in a deae-sephacel column chromatography. deae-frs. 3-6 were sugar-rich glycoprotein fractions which contained galactose and ... | 1990 | 2165527 |
| isolation and characterization of a vicia graminea and vicia unijuga lectins-binding (vgu) glycoprotein with thomsen-friedenreich (t) activity from human liver metastases of pancreas carcinoma. | 1. perchloric acid-soluble fraction from liver metastases of pancreas carcinoma of a patient with blood group b, was subjected to a systematic affinity chromatography using vicia unijuga lectin (vua) and arachis hypogaea anti-t lectin (pna) as immobilized ligands and separated into three fractions, b-active glycoprotein fraction, serologically inactive glycoprotein fraction and glycoprotein (vp) fraction exhibiting reactivities for vicia graminea lectin (vga), vua and pna. 2. vga- and vua-bindin ... | 1992 | 1592152 |
| separation and biochemical characterization of blood group n antigen precursor glycoproteins with thomsen-friedenreich (t) activity, t-active glycoproteins and n antigen precursor glycoproteins from ascites of primary ovarian cancer patients. | 1. three perchloric acid-soluble fractions from ascites of three primary ovarian cancer patients were subjected to sephacryl s-300 gel filtration, respectively, and three fr. 1 which were eluted in the vicinity of void volume as minor fractions, were then separated by a systematic affinity chromatography using vicia unijuga lectin-sepharose cl-4b column and arachis hypogaea lectin-sepharose cl-4b column into three glycoproteins, blood group n antigen precursor glycoprotein with thomsen-friedenre ... | 1990 | 2257955 |
| isolation and characterization of blood group n antigen precursor glycoproteins with thomsen-friedenreich (t) activity, n antigen precursor glycoproteins and t-active glycoproteins from cyst fluids of malignant ovarian clear cell carcinoma. | 1. two perchloric acid-soluble glycoprotein fractions from cyst fluids of 2 patients with ovarian clear cell carcinoma in malignant were subjected to a systematic affinity chromatography using vicia unijuga lectin-cellulofine column and arachis hypogaea lectin-cellulofine column and separated into four glycoproteins, blood group n antigen precursor glycoprotein with thomsen-friedenreich (t) activity, n antigen precursor glycoprotein, t-active glycoprotein and serologically inactive glycoprotein, ... | 1990 | 2282960 |
| the effect of some non-protein amino acids on pollen germination and pollen-tube growth in five species of the vicieae. | the effects of canavanine, α,γ-diaminobutyric acid, homoarginine and lathyrine on the germination of pollen and on in-vitro growth of pollen tubes were studied in the following species: lathyrus niger, l. silvestris, vicia unijuga, pisum sativum and cicer arietinum.the effects of these non-protein amino acids depended on their quantity and on the plant species. every amino acid had a promoting effect on germination and growth at some concentration in some species. inhibition or promotion of poll ... | 1967 | 24522605 |