| in vitro culture of the tropical sponge axinella corrugata (demospongiae): effect of food cell concentration on growth, clearance rate, and biosynthesis of stevensine. | in vitro culture is one possible method for supplying sponge metabolites for pharmaceutical applications, but appropriate feeding regimens that maximize both growth and metabolite biosynthesis are largely unknown. according to the natural concentration (nc) of cells 1 to 50 micro m in size that are available to wild axinella corrugata, we fed explants a multispecific diet of bacteria, microalgae, and yeast at 4 different concentrations: 1nc, 3nc, 5nc, and 5+1nc (the last consisted of 5 nc of bac ... | 2003 | 14564533 |
| characterization of a culturable alphaproteobacterial symbiont common to many marine sponges and evidence for vertical transmission via sponge larvae. | a closely related group of alphaproteobacteria were found to be present in seven genera of marine sponges from several locations and were shown to be transferred between sponge generations through the larvae in one of these sponges. isolates of the alphaproteobacterium were cultured from the sponges axinella corrugata, mycale laxissima, monanchora unguifera, and niphates digitalis from key largo, florida; didiscus oxeata and monanchora unguifera from discovery bay, jamaica; an acanthostronglyoph ... | 2006 | 16672523 |
| development of a red-shifted fluorescence-based assay for sars-coronavirus 3cl protease: identification of a novel class of anti-sars agents from the tropical marine sponge axinella corrugata. | sars-coronavirus (sars-cov) encodes a main protease, 3clpro, which plays an essential role in the viral life cycle and is currently the prime target for discovering new anti-coronavirus agents. in this article, we report our success in developing a novel red-shifted (rs) fluorescence-based assay for 3clpro and its application for identifying small-molecule anti-sars agents from marine organisms. we have synthesised and characterised the first generation of a red-shifted internally quenched fluor ... | 2006 | 16895476 |
| acl-i, a lectin from the marine sponge axinella corrugata: isolation, characterization and chemotactic activity. | the lectin from the marine sponge axinella corrugata (acl-i) was purified by affinity chromatography on rabbit erythrocytic stroma incorporated into a polyacrylamide gel followed by gel filtration on ultrogel aca 44 column. purified acl-i is a hexameric glycoprotein with a mr of 82.3 kda estimated by sds-page and 78.5 kda by fplc on superose 12 hr column. the pi of lectin is 6.3 and acl-i is constituted of 13.9 kda similar subunits some of them linked by disulphide bridges. this lectin agglutina ... | 2008 | 18424185 |
| immunohistochemical localization of an n-acetyl amino-carbohydrate specific lectin (acl-i) of the marine sponge axinella corrugata. | the n-acetyl amino-carbohydrate specific lectin (acl-i) was previously identified and purified by us from the marine sponge axinella corrugata (phylum porifera, class demospongiae). the distribution of the specific lectin within the tissue of the sponge was studied by bright-field optical microscopy immunohistochemistry in order to better understand its physiological role in the sponge. polyclonal antibodies were raised against purified acl-i in mice and tested by western blot technique. the imm ... | 2010 | 20727574 |
| gene recruitment--a common mechanism in the evolution of transfer rna gene families. | the evolution of alloacceptor transfer rnas (trnas) has been traditionally thought to occur vertically and reflect the evolution of the genetic code. yet there have been several indications that a trna gene could evolve horizontally, from a copy of an alloacceptor trna gene in the same genome. earlier, we provided the first unambiguous evidence for the occurrence of such "trna gene recruitment" in nature--in the mitochondrial (mt) genome of the demosponge axinella corrugata. yet the extent and t ... | 2010 | 21195140 |
| biological activities of acl-i and physicochemical properties of acl-ii, lectins isolated from the marine sponge axinella corrugata. | lectin ii from the marine sponge axinella corrugata (acl-ii) was purified by affinity chromatography on rabbit erythrocytic stroma incorporated into a polyacrylamide gel, followed by gel filtration on ultrogel aca 44 column. purified acl-ii is a lectin with an mr of 80kda and 78kda, estimated by sds-page and by fplc on superose 12 hr column, respectively. acl-ii mainly agglutinates native rabbit erythrocytes and this hemagglutinating activity is independent of ca(2+), mg(2+) and mn(2+), but is i ... | 2012 | 22245532 |
| corrugoside, a new immunostimulatory alpha-galactoglycosphingolipid from the marine sponge axinella corrugata. | corrugoside (1a), a new immunostimulatory triglycosilated alpha-galactoglycosphingolipid, was isolated from the marine sponge axinella corrugata, and its structure determined by spectral analysis and chemical degradation. compound 1a activated murine nkt cells in vitro, with a potency of about 2 logs lower than that of alphagalcer. four stereoisomeric glycosphingolipids (2a-2d) were also obtained, beta-glucosylceramides bearing unusual endoperoxide and allylic hydroperoxide functionalities on th ... | 2008 | 18037298 |
| pyrosequencing of bacterial symbionts within axinella corrugata sponges: diversity and seasonal variability. | marine sponge species are of significant interest to many scientific fields including marine ecology, conservation biology, genetics, host-microbe symbiosis and pharmacology. one of the most intriguing aspects of the sponge "holobiont" system is the unique physiology, interaction with microbes from the marine environment and the development of a complex commensal microbial community. however, intraspecific variability and temporal stability of sponge-associated bacterial symbionts remain relativ ... | 2012 | 22701613 |
| staining tumor cells with biotinylated acl-i, a lectin isolated from the marine sponge, axinella corrugata. | axinella corrugata lectin 1 (acl-1) was purified from aqueous extracts of the marine sponge, axinella corrugata. acl-1 strongly agglutinates native rabbit erythrocytes. the hemagglutination is inhibited by n-acetyl derivatives, particularly n, n', n"-triacetylchitotriose, n-acetyl-d-glucosamine, n-acetyl-d-mannosamine and n-acetyl-d-galactosamine. we investigated the capacity of biotinylated acl-1 to stain several transformed cell lines including breast (t-47d, mcf7), colon (ht-29), lung (h460), ... | 2013 | 22954064 |
| marine sponges (porifera: demospongiae) from the gulf of méxico, new records and redescription of erylus trisphaerus (de laubenfels, 1953). | marine sponges usually constitute the most diverse group of the benthic community in coral reefs. although they are reasonably well studied at the northern gulf of mexico (gmx), the southern gmx is poorly known and lacks records from many major reef systems that lie off the mexican coast. the present taxonomic study is the first sponge account from alacranes reef, the largest coral reef system in the gmx, and from the shallow reef banks of sisal, both in the northwest yucatan peninsula. the 19 s ... | 2015 | 25661604 |
| conservation and phylogeny of a novel family of non-hox genes of the antp class in demospongiae (porifera). | a survey across the most basal animal phylum, the porifera, for the presence of homeobox-containing genes led to the isolation of 24 partial or complete homeobox sequences from 21 sponge species distributed in 15 families and 6 orders of demospongiae. all the new sequences shared a high identity/similarity with emh-3 (ephydatia muelleri), a non-hox gene from the antp class. the demox sequences, emh-3, and related homeodomains formed a well-supported clade with no true affinity with any known bil ... | 2006 | 16786434 |
| transfer rna gene recruitment in mitochondrial dna. | transfer rna (trna) is the adaptor molecule that mediates recognition of the codon sequence in mrna and enables its translation into the appropriate amino acid. accordingly, phylogenetic relationships among trna genes are often thought to recapitulate the evolution of the genetic code. however, it has been demonstrated experimentally that one trna gene can be replaced with a copy of another carrying a single mutation in its anticodon sequence. in this article, we show that such "gene recruitment ... | 2005 | 15734570 |
| characterization of genetic markers for in vitro cell line identification of the marine sponge axinella corrugata. | the marine sponge axinella corrugata is being developed as a model organism for in vitro marine invertebrate research. molecular genetics methods such as dna fingerprinting [amplified fragment length polymorphism (aflp) and single-stranded conformation polymorphism (sscp)] and single-locus dna sequence analyses were applied to this model to meet the primary objective of identifying positive a. corrugata-specific molecular markers that will aid in verifying cell identity in vitro and distinguish ... | 2006 | 12011172 |