| sphingomonas melonis sp. nov., a novel pathogen that causes brown spots on yellow spanish melon fruits. | a polyphasic taxonomic study was performed on the phytopathogenic bacterial strains dapp-pg 224(t) and dapp-pg 228, which cause brown spot on yellow spanish melon (cucumis melo var. inodorus) fruits. based on the presence of glucuronosyl ceramide (sgl-1) in cellular lipids, the results of fatty acid analysis and 16s rdna sequence comparison, the strains had been identified as belonging to the genus sphingomonas and as phylogenetically related to sphingomonas mali, sphingomonas pruni and sphingom ... | 2002 | 12508872 |
| description of two novel species, sphingomonas abaci sp. nov. and sphingomonas panni sp. nov. | two gram-negative, rod-shaped, non-spore-forming bacterial strains designated c42t and c52t were isolated in the medical clinic for small animals and ungulates at the university for veterinary medicine vienna, austria. on the basis of 16s rrna gene sequence similarity, both strains were shown to belong to the genus sphingomonas. strain c42t showed the greatest levels of sequence similarity with sphingomonas melonis dsm 14444t and sphingomonas aquatilis kctc 2881t (both 97.7 %). strain c52t showe ... | 2005 | 16280528 |
| sphingomonas astaxanthinifaciens sp. nov., a novel astaxanthin-producing bacterium of the family sphingomonadaceae isolated from misasa, tottori, japan. | a red-pigmented, gram-negative, motile, strictly aerobic, mesophilic, oval- or short rod-shaped bacterium (tdma-17(t)) was isolated from fresh water collected at misasa, a radioactive site in japan. tdma-17(t) was slightly tolerant against gamma-ray irradiation, and effectively produced carotenoids (2.8 mg g(-1) dry cells) including, astaxanthin and astaxanthin isomers. phylogenetic analysis based on 16s rrna gene sequences placed tdma-17(t) in a distinct lineage in the family sphingomonadaceae, ... | 2007 | 17590230 |
| sphingomonas jaspsi sp. nov., a novel carotenoid-producing bacterium isolated from misasa, tottori, japan. | a yellow-pigmented, gram-negative, motile, strictly aerobic, pleomorphic bacterium (strain tdma-16(t)) was isolated from a freshwater sample collected at misasa (tottori, japan). strain tdma-16(t) was slightly tolerant to gamma-ray irradiation and produced carotenoids, including zeaxanthin, nostoxanthin and an unknown carotenoid, effectively [1.7 mg (g dry cells)(-1)]. the dna g+c content of strain tdma-16(t) was 63.3 mol%. phylogenetic analysis based on 16s rrna gene sequences placed strain tdm ... | 2007 | 17625171 |
| novel methylotrophic bacteria isolated from the river thames (london, uk). | enrichment and elective culture for methylotrophs from sediment of the river thames in central london yielded a diversity of pure cultures representing several genera of gram-negative and gram-positive bacteria, which were mainly of organisms not generally regarded as typically methylotrophic. substrates leading to successful isolations included methanol, monomethylamine, dimethylamine, trimethylamine, methanesulfonate and dimethylsulfone. several isolates were studied in detail and shown by the ... | 2008 | 18681896 |
| sphingomonas rubra sp. nov., isolated from bioreactor wastewater. | a gram-reaction-negative, rod-shaped, motile, neutrophilic bacterium, designated strain bh3(t), was isolated from wastewater of a sequential batch reactor treating wastewater taken from a leather plant. the isolate grew in 0-8 % (w/v) nacl, at ph 6-9 and at 4-45 °c. chemotaxonomic analysis showed that strain bh3(t) had characteristics typical of members of the genus sphingomonas, such as the presence of sphingolipids, q-10 and 2-hydroxymyristic acid and the absence of 3-hydroxy fatty acids. the ... | 2011 | 20511462 |
| atp modulates the growth of specific microbial strains. | the regulatory function of extracellular atp (exatp) in bacteria is unknown, but recent studies have demonstrated exatp induced enhanced secondary metabolite production and morphological differentiation in streptomyces coelicolor. the growth of streptomyces coelicolor, however, was unaffected by exatp, although changes in growth are common phenotypes. to identify bacteria whose growth is altered by exatp, we measured exatp-induced population changes in fast-growing microbes and actinomycetes in ... | 2011 | 20512647 |
| two-tiered histidine kinase pathway involved in heat shock and salt sensing in the general stress response of sphingomonas melonis fr1. | the general stress response (gsr) allows bacteria to monitor and defend against a broad set of unrelated, adverse environmental conditions. in alphaproteobacteria, the key step in gsr activation is phosphorylation of the response regulator phyr. in sphingomonas melonis fr1, seven phyr-activating kinases (paks), paka to pakg, are thought to directly phosphorylate phyr under different stress conditions, but the nature of the activating signals remains obscure. pakf, a major sensor of nacl and heat ... | 2015 | 25666137 |
| complex two-component signaling regulates the general stress response in alphaproteobacteria. | the general stress response (gsr) in alphaproteobacteria was recently shown to be controlled by a partner-switching mechanism that is triggered by phosphorylation of the response regulator phyr. activation of phyr ultimately results in release of the alternative extracytoplasmic function sigma factor σ(ecfg), which redirects transcription toward the gsr. little is known about the signal transduction pathway(s) controlling phyr phosphorylation. here, we identified the single-domain response regul ... | 2014 | 25404331 |
| characterization of a novel nicotine degradation gene cluster ndp in sphingomonas melonis ty and its evolutionary analysis. | sphingomonas melonis ty utilizes nicotine as a sole source of carbon, nitrogen, and energy through a variant of the pyridine and pyrrolidine pathways (vpp). a 31-kb novel nicotine-degrading gene cluster, ndp, in strain ty exhibited a different genetic organization with the vpp cluster in strains ochrobactrum rhizosphaerae sjy1 and agrobacterium tumefaciens s33. genes in vpp were separated by a 20-kb interval sequence, while genes in ndp were localized together. half of the homolog genes were in ... | 2017 | 28337179 |
| two novel sets of genes essential for nicotine degradation by sphingomonas melonis ty. | nicotine is a type of environmental pollutant present in the tobacco waste that is generated during tobacco manufacturing. sphingomonas melonis ty can utilize nicotine as a sole source of carbon, nitrogen and energy via a variant of the pyridine and pyrrolidine pathway (the vpp pathway). in this study, we report the identification of two novel sets of genes, ndra1a2a3, and ndrb1b2b3b4, which are crucial for nicotine degradation by strain ty. ndra1a2a3 and ndrb1b2b3b4 exhibit similarity with both ... | 2016 | 28144232 |
| systems-level proteomics of two ubiquitous leaf commensals reveals complementary adaptive traits for phyllosphere colonization. | plants are colonized by a diverse community of microorganisms, the plant microbiota, exhibiting a defined and conserved taxonomic structure. niche separation based on spatial segregation and complementary adaptation strategies likely forms the basis for coexistence of the various microorganisms in the plant environment. to gain insights into organism-specific adaptations on a molecular level, we selected two exemplary community members of the core leaf microbiota and profiled their proteomes upo ... | 2016 | 27457762 |
| the arabidopsis leaf transcriptome reveals distinct but also overlapping responses to colonization by phyllosphere commensals and pathogen infection with impact on plant health. | plants are colonized by a variety of bacteria, most of which are not pathogenic. currently, the plant responses to phyllosphere commensals or to pathogen infection in the presence of commensals are not well understood. here, we examined the transcriptional response of arabidopsis thaliana leaves to colonization by common commensal bacteria in a gnotobiotic system using rna sequencing and conducted plant mutant assays. arabidopsis responded differently to the model bacteria sphingomonas melonis f ... | 2016 | 27306148 |
| metabolic footprint of epiphytic bacteria on arabidopsis thaliana leaves. | the phyllosphere, which is defined as the parts of terrestrial plants above the ground, is a large habitat for different microorganisms that show a high extent of adaption to their environment. a number of hypotheses were generated by culture-independent functional genomics studies to explain the competitiveness of specialized bacteria in the phyllosphere. in contrast, in situ data at the metabolome level as a function of bacterial colonization are lacking. here, we aimed to obtain new insights ... | 2016 | 26305156 |
| synthetic vanillate-regulated promoter for graded gene expression in sphingomonas. | regulated promoters are an important basic genetic tool allowing, for example, gene-dosage and gene depletion studies. we have previously described a cumate-inducible promoter (p(q5)) that is functional in diverse alphaproteobacteria. this promoter has been engineered by combining a synthetic minimal promoter, p(syn2), and operator sites and the repressor of the pseudomonas putida f1 cym/cmt system. in the present study, we engineered a vanillate-regulated promoter using p(syn2) and the regulato ... | 2014 | 25262659 |
| the branched ccsa/ccka-chpt-ctra phosphorelay of sphingomonas melonis controls motility and biofilm formation. | the ccka-chpt-ctra phosphorelay is central to the regulation of the cell cycle in caulobacter crescentus. the three proteins are conserved in alphaproteobacteria, but little is known about their roles in most members of this class. here, we characterized the system in sphingomonas melonis. we found that the transcription factor ctra is the master regulator of flagella synthesis genes, the hierarchical transcriptional organization of which is herein described. ctra also regulates genes involved i ... | 2015 | 25825287 |
| sphingomonas kyungheensis sp. nov., a bacterium with ginsenoside-converting activity isolated from soil of a ginseng field. | a bacterial strain thg-b283(t), which has β-glucosidase activity, was isolated from soil of a ginseng field. cells were gram-reaction-negative, oxidase- and catalase-positive, aerobic, motile with one polar flagellum and rod-shaped. the strain was subjected to a polyphasic taxonomic study. strain thg-b283(t) grew optimally at around ph 7.0, at 25-28 °c and in the absence of nacl on r2a agar. 16s rrna gene sequence analysis revealed that strain thg-b283(t) belongs to the family sphingomonadaceae ... | 2013 | 23687058 |