| pigmentiphaga kullae gen. nov., sp. nov., a novel member of the family alcaligenaceae with the ability to decolorize azo dyes aerobically. | the taxonomic position of pseudomonas strain k24, which was isolated previously after an aerobic enrichment with the azo compound 1-(4'-carboxyphenylazo)-4-naphthol as the sole source of carbon and energy, was investigated. the detection of a quinone system with ubiquinone q-8 as the predominant compound and a polyamine pattern with putrescine and 2-hydroxyputrescine as the major polyamines present suggested that strain k24t belongs to the beta-subclass of the proteobacteria. this was supported ... | 2001 | 11594620 |
| cloning and characterization of the gene coding for the aerobic azoreductase from pigmentiphaga kullae k24. | the gene coding for an aerobic azoreductase was cloned from pigmentiphaga kullae k24, which is able to grow with the carboxylated azo compound 1-(4'-carboxyphenylazo)-4-naphthol (carboxy-orange i) as sole source of carbon and energy. the gene encoded a protein with a molecular weight of 20,557 da, with a conserved putative nad(p)h-binding site in the amino-terminal region. the deduced amino acid sequence showed no further significant sequence homologies to previously studied aerobic azoreductase ... | 2003 | 12719939 |
| kerstersia gyiorum gen. nov., sp. nov., a novel alcaligenes faecalis-like organism isolated from human clinical samples, and reclassification of alcaligenes denitrificans rüger and tan 1983 as achromobacter denitrificans comb. nov. | a polyphasic taxonomic study was performed on nine isolates recovered from various human clinical samples. phenotypically, these isolates resembled alcaligenes faecalis. whole-cell protein analysis distinguished two different species, and this was confirmed by dna-dna hybridizations. cellular fatty acid analysis and 16s rdna sequence analysis indicated that these isolates were related to the genera alcaligenes, bordetella, achromobacter and pigmentiphaga and belonged to the family alcaligenaceae ... | 2003 | 14657111 |
| advenella incenata gen. nov., sp. nov., a novel member of the alcaligenaceae, isolated from various clinical samples. | a polyphasic taxonomic study of 14 isolates recovered from various human and veterinary clinical samples was performed. phenotypically these isolates shared several characteristics with members of the alcaligenaceae and related genera. random amplified polymorphic dna fingerprinting and whole-cell protein analysis suggested the presence of multiple genomic groups, which was confirmed by dna-dna hybridization experiments. 16s rrna gene sequence analysis indicated that these isolates were related ... | 2005 | 15653883 |
| tetrathiobacter kashmirensis gen. nov., sp. nov., a novel mesophilic, neutrophilic, tetrathionate-oxidizing, facultatively chemolithotrophic betaproteobacterium isolated from soil from a temperate orchard in jammu and kashmir, india. | twelve chemolithotrophic strains were isolated from temperate orchard soil on reduced sulfur compounds as energy and electron sources and characterized on the basis of their physiological properties and ability to oxidize various reduced sulfur compounds. the new isolates could oxidize tetrathionate as well as thiosulfate, and oxidation of the latter involved conversion of thiosulfate to tetrathionate followed by its accumulation and eventual oxidation to sulfate, manifested in the production of ... | 2005 | 16166666 |
| mutational analysis of the metabolism of 2,6-naphthalenedisulfonate by pigmentiphaga sp. nds-2. | the metabolism of 2,6-naphthalenedisulfonate (26nds) by pigmentiphaga sp. nds-2 was analyzed by isolating a mutant (nds2-002) which slowly grew on a minimal medium containing 26nds as the sole source of carbon. liquid chromatography/mass spectrometry (lc/ms) analysis of metabolic intermediates in this strain revealed that 5-sulfosalicylate (5ssa) is accumulated during the degradation of 26nds. to analyze the lower metabolic pathway, a mutant strain nds2-008, which could not grow either on 26nds, ... | 2003 | 16233443 |
| pigmentiphaga daeguensis sp. nov., isolated from wastewater of a dye works, and emended description of the genus pigmentiphaga. | a gram-negative, non-spore-forming, rod-shaped pigmentiphaga-like bacterial strain, k110(t), was isolated from wastewater collected from a dye works in korea and was subjected to a polyphasic taxonomic analysis. strain k110(t) grew optimally at ph 7.0--8.0 and 37 degrees c in the presence of 0.5 % (w/v) nacl. it contained q-8 as the predominant ubiquinone and c(16 : 0), cyclo c(17 : 0) and cyclo c(19 : 0)omega8c as the major fatty acids. the major polar lipids were phosphatidylglycerol, phosphat ... | 2007 | 17551027 |
| diversity of bacterial isolates from commercial and homemade composts. | the diversity of heterotrophic bacterial isolates of three commercial and two homemade composts was studied. the commercial composts were produced from poultry litter (pc), sewage sludge (sc), municipal solid waste (mc), and homemade composts (thermal compost [dc] and vermicompost [vc]) from food wastes. the taxonomic and physiological diversity of the heterotrophic culturable bacteria was assessed using phenotypic and genotypic characterization and the analysis of the partial 16s rrna gene sequ ... | 2008 | 17876655 |
| pigmentiphaga litoralis sp. nov., a facultatively anaerobic bacterium isolated from a tidal flat sediment. | a novel gram-negative, facultatively anaerobic, non-sporulating, non-motile, catalase- and oxidase-positive, rod-shaped bacterium (strain jsm 061001(t)) was isolated from a tidal flat in the south china sea, china. growth occurred with 0-5 % (w/v) nacl [optimum, 0.5-1 % (w/v) nacl], at ph 5.0-10.0 (optimum, ph 7.0) and at 4-35 degrees c (optimum, 25-30 degrees c). the major cellular fatty acids were c(16 : 0), cyclo c(17 : 0), c(18 : 1)omega7c and c(16 : 1). strain jsm 061001(t) contained ubiqui ... | 2009 | 19244433 |
| parapusillimonas granuli gen. nov., sp. nov., isolated from granules from a wastewater-treatment bioreactor. | a novel betaproteobacterium, designated strain ch07(t), was isolated from granules from the wastewater-treatment bioreactor of an alcohol fermentation factory in south korea. in order to determine its taxonomic position, the novel strain was characterized using a polyphasic approach. the new strain was gram-negative, facultatively anaerobic, non-spore-forming, motile and short rod-shaped. 16s rrna gene sequence analysis revealed that strain ch07(t) belonged to the class betaproteobacteria, being ... | 2010 | 19671719 |
| eiseniicola composti gen. nov., sp. nov., with antifungal activity against plant pathogenic fungi. | a gram-negative, short rod-shaped bacterial strain, yc06271t, was isolated from the vermicompost (vc) collected at masan, korea and its taxonomic position was investigated by a polyphasic taxonomic approach. strain yc06271t grew optimally at 28-30 degrees c and at ph 7.0-9.0. the 16s rrna gene sequence of strain yc06271t was most closely related to members of the genera bordetella (96.4-95.8 %), achromobacter (96.0-95.7 %), alcaligenes (96.0-94.2 %), pusillimonas noertemannii (95.9 %), pigmentip ... | 2010 | 19734285 |
| pusillimonas ginsengisoli sp. nov., isolated from soil of a ginseng field. | two novel strains of gram-negative, non-sporulating, short rod-shaped, motile bacteria, designated dcy25t and dcy28, were isolated from soil of a ginseng field in south korea and characterized in order to determine their taxonomic position. 16s rrna gene sequence analysis showed that strains dcy25t and dcy28 belonged to the betaproteobacteria, the highest sequence similarities being found with pusillimonas noertemannii bn9t (96.9%), bordetella trematum dsm 11334(t) (95.9%), achromobacter denitri ... | 2010 | 19749028 |
| identification and molecular characterization of a novel flavin-free nadph preferred azoreductase encoded by azob in pigmentiphaga kullae k24. | microbial degradation of azo dyes is commonly initiated by the reduction of the azo bond(s) by a group of nadh or nadph dependant azoreductases with many requiring flavin as a cofactor. in this study, we report the identification of a novel flavin-free nadph preferred azoreductase encoded by azob in pigmentiphaga kullae k24. | 2010 | 20233432 |
| multiple dna extractions coupled with stable-isotope probing of anthracene-degrading bacteria in contaminated soil. | in many of the dna-based stable-isotope probing (sip) studies published to date in which soil communities were investigated, a single dna extraction was performed on the soil sample, usually using a commercial dna extraction kit, prior to recovering the (13)c-labeled (heavy) dna by density-gradient ultracentrifugation. recent evidence suggests, however, that a single extraction of a soil sample may not lead to representative recovery of dna from all of the organisms in the sample. to determine w ... | 2011 | 21398486 |
| stable-isotope probing of the polycyclic aromatic hydrocarbon-degrading bacterial guild in a contaminated soil. | the bacteria responsible for the degradation of naphthalene, phenanthrene, pyrene, fluoranthene or benz[a]anthracene in a polycyclic aromatic hydrocarbon (pah)-contaminated soil were investigated by dna-based stable-isotope probing (sip). clone libraries of 16s rrna genes were generated from the (13) c-enriched ('heavy') dna recovered from each sip experiment, and quantitative pcr primers targeting the 16s rrna gene were developed to measure the abundances of many of the sip-identified sequences ... | 2011 | 21564459 |
| paralcaligenes ureilyticus gen. nov., sp. nov. isolated from soil of a korean ginseng field. | a bacterial strain, designated gr24-5(t), was isolated from soil cultivated with korean ginseng. cells were gram-negative, strictly aerobic, catalase- and oxidase-positive, non-spore-forming motile rods. based on the 16s rrna gene sequence, strain gr24-5(t) could be assigned to the family alcaligenaceae. strain gr24-5(t) showed the highest sequence similarities with parapusillimonas granuli ch07(t) (97.1%), pusillimonas noertemannii bn9(t) (96.9%), pigmentiphaga kullae dsm 13608(t) (96.5%), and ... | 2011 | 21717340 |
| pigmentiphaga soli sp. nov., a bacterium isolated from soil. | strain bs12(t), a gram-negative motile bacterium, was isolated from soil in south korea and characterized to determine its taxonomic position. phylogenetic analyses based on the 16s rrna gene sequence revealed that the strain belonged to the family alcaligenaceae in the class betaproteobacteria. the highest degree of sequence similarities of strain bs12(t) were found with pigmentiphaga litoralis jsm 061001(t) (98.3%), pigmentiphaga daeguensis k110(t) (98.2%), and pigmentiphaga kullae k24(t) (98. ... | 2011 | 22068507 |