| additional evidence that juvenile oyster disease is caused by a member of the roseobacter group and colonization of nonaffected animals by stappia stellulata-like strains. | juvenile oyster disease (jod) causes significant annual mortalities of hatchery-produced eastern oysters, crassostrea virginica, cultured in the northeast. we have reported that a novel species of the alpha-proteobacteria roseobacter group (designated cvsp) was numerically dominant in jod-affected animals sampled during the 1997 epizootic on the damariscotta river, maine. in this study we report the isolation of cvsp bacteria from jod-affected oysters during three separate epizootics in 1998. th ... | 2000 | 10966410 |
| biofilm growth kinetics of a monomethylamine producing alphaproteobacteria strain isolated from an anaerobic reactor. | industrial fishing effluents are characterized by high loads of protein and sulfate that stimulate the activity of proteolytic and sulfate reducing bacteria during anaerobic digestion. their metabolic products (nh3 and h2s respectively) have a well-known detrimental effect on the activity of methanogens. since methylamine is a carbon source used by methylaminotrophic methane producing archaea (mmpa) but not by sulfate reducing bacteria (srb), enriched mmpa anaerobic biofilms have been developed ... | 2010 | 19446030 |
| [screening and characterization of antibacterial and cytotoxic marine bacteria associated with karlodinium micrum]. | antibacterial and cytotoxic activities were screened for marine bacteria which have been isolated from karlodinium micrum, in order to obtain potential strains with antibacterial and cytotoxic activities. | 2010 | 20931872 |
| molecular genetic analysis of a dimethylsulfoniopropionate lyase that liberates the climate-changing gas dimethylsulfide in several marine alpha-proteobacteria and rhodobacter sphaeroides. | the alpha-proteobacterium sulfitobacter ee-36 makes the gas dimethylsulfide (dms) from dimethylsulfoniopropionate (dmsp), an abundant antistress molecule made by many marine phytoplankton. we screened a cosmid library of sulfitobacter for clones that conferred to other bacteria the ability to make dms. one gene, termed dddl, was sufficient for this phenotype when cloned in pet21a and introduced into escherichia coli. close dddl homologues exist in the marine alpha-proteobacteria fulvimarina, lok ... | 2008 | 18237308 |
| mesaconyl-coenzyme a hydratase, a new enzyme of two central carbon metabolic pathways in bacteria. | the coenzyme a (coa)-activated c5-dicarboxylic acids mesaconyl-coa and beta-methylmalyl-coa play roles in two as yet not completely resolved central carbon metabolic pathways in bacteria. first, these compounds are intermediates in the 3-hydroxypropionate cycle for autotrophic co2 fixation in chloroflexus aurantiacus, a phototrophic green nonsulfur bacterium. second, mesaconyl-coa and beta-methylmalyl-coa are intermediates in the ethylmalonyl-coa pathway for acetate assimilation in various bacte ... | 2008 | 18065535 |
| stappia indica sp. nov., isolated from deep seawater of the indian ocean. | a taxonomic study was carried out on strain b106(t), which was isolated from a polycyclic aromatic hydrocarbon-degrading consortium, enriched with deep seawater from the indian ocean. the isolate was gram-negative, oxidase- and catalase-positive, rod-shaped and motile by means of one polar flagellum. growth was observed at salinities of 0.5-11 % and at temperatures of 4-42 degrees c, and the strain was capable of nitrate reduction, but was unable to degrade tween 80 or gelatin. phylogenetic anal ... | 2010 | 19656920 |
| phylogenomics and signature proteins for the alpha proteobacteria and its main groups. | alpha proteobacteria are one of the largest and most extensively studied groups within bacteria. however, for these bacteria as a whole and for all of its major subgroups (viz. rhizobiales, rhodobacterales, rhodospirillales, rickettsiales, sphingomonadales and caulobacterales), very few or no distinctive molecular or biochemical characteristics are known. | 2007 | 18045498 |
| alkaliphilic and halophilic hydrocarbon-utilizing bacteria from kuwaiti coasts of the arabian gulf. | green animate materials from the intertidal zone of the arabian gulf coast accommodated more alkaliphilic and halophilic bacteria than inanimate materials. the alkaliphilic oil-utilizing bacteria, as identified by their 16s ribonucleic acid sequences, belonged to the following genera arranged in decreasing frequences: marinobacter, micrococcus, dietzia, bacillus, oceanobacillus, and citricoccus. the halophilic oil-utilizing bacteria belonged to the genera: marinobacter, georgenia, microbacterium ... | 2007 | 17710391 |
| description of labrenzia alexandrii gen. nov., sp. nov., a novel alphaproteobacterium containing bacteriochlorophyll a, and a proposal for reclassification of stappia aggregata as labrenzia aggregata comb. nov., of stappia marina as labrenzia marina comb. nov. and of stappia alba as labrenzia alba comb. nov., and emended descriptions of the genera pannonibacter, stappia and roseibium, and of the species roseibium denhamense and roseibium hamelinense. | a slightly pink-coloured strain, strain dfl-11(t), was isolated from single cells of the marine dinoflagellate alexandrium lusitanicum and was found to contain the genes encoding two proteins of the photosynthetic reaction centre, pufl and pufm. 16s rrna gene sequence analysis revealed that the novel strain belonged to the alpha-2 subgroup of the proteobacteria and was most closely related to stappia aggregata (97.7 % similarity), stappia alba (98.0 %) and stappia marina (98.0 %). dark-grown cel ... | 2007 | 17473266 |
| molecular and culture-based analyses of aerobic carbon monoxide oxidizer diversity. | isolates belonging to six genera not previously known to oxidize co were obtained from enrichments with aquatic and terrestrial plants. dna from these and other isolates was used in pcr assays of the gene for the large subunit of carbon monoxide dehydrogenase (coxl). coxl and putative coxl fragments were amplified from known co oxidizers (e.g., oligotropha carboxidovorans and bradyrhizobium japonicum), from novel co-oxidizing isolates (e.g., aminobacter sp. strain cox, burkholderia sp. strain lu ... | 2003 | 14660374 |
| physiological, ecological, and phylogenetic characterization of stappia, a marine co-oxidizing bacterial genus. | bacteria play a major role in marine co cycling, yet very little is known about the microbes involved. thirteen co-oxidizing stappia isolates obtained from existing cultures, macroalgae, or surf samples representing geographically and ecologically diverse habitats were characterized using biochemical, physiological, and phylogenetic approaches. all isolates were aerobic chemoorganotrophs that oxidized co at elevated (1,000 ppm) and ambient-to-subambient concentrations (<0.3 ppm). all contained t ... | 2007 | 17142374 |
| nitrate-dependent anaerobic carbon monoxide oxidation by aerobic co-oxidizing bacteria. | two dissimilatory nitrate-reducing (burkholderia xenovorans lb400 and xanthobacter sp. str. cox) and two denitrifying isolates (stappia aggregata iam 12614 and bradyrhizobium sp. str. cpp), previously characterized as aerobic co oxidizers, consumed co at ecologically relevant levels (<100 ppm) under anaerobic conditions in the presence, but not absence, of nitrate. none of the isolates were able to grow anaerobically with co as a carbon or energy source, however, and nitrate-dependent anaerobic ... | 2006 | 16542399 |
| stappia alba sp. nov., isolated from mediterranean oysters. | three bacterial strains isolated from oysters recovered at the spanish mediterranean coast have been phenotypically and genetically characterized. the results of the phylogenetic analysis based on almost complete 16s rdna sequences clustered all three strains together with 99.9% average sequence similarity and situated them in the neighbourhood of the genera stappia, roseibium and pannonibacter, stappia aggregata being their closest neighbour with sequence similarities between 98.8% and 98.9%. d ... | 2005 | 16261856 |
| stappia marina sp. nov., a marine bacterium isolated from the yellow sea. | a gram-negative, aerobic and halophilic bacterium designated strain mano18t was isolated from a tidal flat area of dae-chun, chung-nam, korea. this strain was motile by means of polar flagella, occasionally forming rosette-like aggregates, reduced nitrate to nitrite, required sodium ions for growth, exhibited catalase and oxidase activities and contained q-10 as the major quinone and c(18 : 1)omega7c as the dominant cellular fatty acid. analysis of the 16s rrna gene sequence revealed that this s ... | 2006 | 16403869 |
| reclassification of marine agrobacterium species: proposals of stappia stellulata gen. nov., comb. nov., stappia aggregata sp. nov., nom. rev., ruegeria atlantica gen. nov., comb. nov., ruegeria gelatinovora comb. nov., ruegeria algicola comb. nov., and ahrensia kieliense gen. nov., sp. nov., nom. rev. | the bootstrapped 16s rdna sequence-based neighbor-joining phylogeny has suggested that the marine species of the genus agrobacterium have no relation to the terrestrial agrobacterium species. agrobacterium atlanticum iam 14463(t) (a superscript (t)=type strain), agrobacterium ferrugineum iam 12616(t), agrobacterium gelatinovorum iam 12617(t), agrobacterium meteori iam 14464(t), agrobacterium stellulatum iam 12621(t) and iam 12614, and the invalidly published marine species "agrobacterium kielien ... | 1998 | 12501429 |
| diversity of the ring-cleaving dioxygenase gene pcah in a salt marsh bacterial community. | degradation of lignin-related aromatic compounds is an important ecological process in the highly productive salt marshes of the southeastern united states, yet little is known about the mediating organisms or their catabolic pathways. here we report the diversity of a gene encoding a key ring-cleaving enzyme of the beta-ketoadipate pathway, pcah, amplified from bacterial communities associated with decaying spartina alterniflora, the salt marsh grass that dominates these coastal systems, as wel ... | 2001 | 11722937 |
| roseigium denhamense gen. nov., sp. nov. and roseibium hemelinense sp. nov., aerobic bacteriochlorophyll-containing bacteria isolated from the east and west coasts of australia. | phenotypic and phylogenetic studies were performed with 10 strains of bacteriochlorophyll-containing bacteria isolated from a variety of marine environments (surface of rhodophyta, sand and algal sand mat) on the east and west coasts of australia. the strains were aerobic, chemoheterotrophic, gram-negative, motile rods with peritrichous flagella. bacteriochlorophyll a was synthesized under aerobic conditions. catalase, nitrate reductase, oxidase and phosphatase were produced. onpg reaction was p ... | 2000 | 11155991 |
| compositions of microbial communities associated with oil and water in a mesothermic oil field. | samples of produced water and oil obtained from the enermark field (near medicine hat, alberta, canada) were separated into oil and aqueous phases first gravitationally and then through centrifugation at 20°c in an atmosphere of 90% n(2) and 10% co(2). biomass that remained associated with oil after gravitational separation (1×g) was dislodged by centrifugation at 25,000×g. dna was isolated from the aqueous and oil-associated biomass fractions and subjected to polymerase chain reaction amplifica ... | 2011 | 22038128 |
| induction and resuscitation of the viable but nonculturable state in a cyanobacteria-lysing bacterium isolated from cyanobacterial bloom. | the viable but nonculturable (vbnc) state has been found to be a growth strategy used by many aquatic pathogens; however, few studies have focused on vbnc state on other aquatic bacterial groups. the purpose of this study was to explore the vbnc state of cyanobacteria-lysing bacteria and the conditions that regulate their vbnc state transformation. three cyanobacteria-lysing heterotrophic bacterial strains (f1, f2 and f3) were isolated with liquid infection method from a lake that has experience ... | 2011 | 21850447 |
| rubrimonas shengliensis sp. nov. and polymorphum gilvum gen. nov., sp. nov., novel members of alphaproteobacteria from crude oil contaminated saline soil. | four bacterial strains were isolated from a crude oil contaminated saline soil in shengli oilfield, china. strains sl014b-28a2(t) and sl014b-80a1 were most closely related to rubrimonas cliftonensis och 317(t), while strains sl003b-26a1(t) and sl003b-26a2 were most closely related to but readily different from the species in the pannonibacter-labrenzia-roseibium-stappia cluster. the major fatty acids were c(18:1)ω7c, c(16:0), c(18:0) and 11-methyl c(18:1)ω7c, and c(18:1)ω7c, 11-methyl c(18:1)ω7c ... | 2011 | 21600718 |
| the phytoplankton nannochloropsis oculata enhances the ability of roseobacter clade bacteria to inhibit the growth of fish pathogen vibrio anguillarum. | phytoplankton cultures are widely used in aquaculture for a variety of applications, especially as feed for fish larvae. phytoplankton cultures are usually grown in outdoor tanks using natural seawater and contain probiotic or potentially pathogenic bacteria. some roseobacter clade isolates suppress growth of the fish pathogen vibrio anguillarum. however, most published information concerns interactions between probiotic and pathogenic bacteria, and little information is available regarding the ... | 2011 | 22053210 |
| agaricicola taiwanensis gen. nov., sp. nov., an alphaproteobacterium isolated from the edible mushroom agaricus blazei. | a gram-negative, beige-pigmented, aerobic, motile, club-shaped bacterium, designated strain cc-sbabm117(t), was isolated from the stipe of the edible mushroom agaricus blazei murrill. 16s rrna gene sequence analysis demonstrated that the strain shared <93 % similarity with the type strains of species in the genera pannonibacter, methylopila, nesiotobacter and stappia. the organism was unable to produce acid from carbohydrates, but utilized a number of organic acids and amino acids. ubiquinone 10 ... | 2010 | 19819997 |
| pannonibacter phragmitetus gen. nov., sp. nov., a novel alkalitolerant bacterium isolated from decomposing reed rhizomes in a hungarian soda lake. | three alkalitolerant bacterial strains were isolated from the surface of decomposing rhizomes of reed [phragmites australia (cav.) trin. et steudel] in lake fertö (hungary). cells of the novel isolates were gram-negative, motile rods and formed star-shaped aggregates. they were facultatively anaerobic and chemo-organotrophic. bacteriochlorophyll a was not synthesized under aerobic conditions. the strains were catalase and oxidase positive, produced acid from d-glucose under aerobic and anaerobic ... | 2003 | 12710626 |
| stappia aquimarina sp. nov., isolated from seawater. | a light-yellow pigmented bacterial strain designated spio-19(t) was isolated from seawater in taiwan and was characterized using the polyphasic taxonomy approach. strain spio-19(t) was gram-negative, obligately aerobic, rod-shaped, motile by means of one polar flagellum and non-spore forming. growth occurred at 15-42°c (optimum, 30-40°c), at ph 5.0-9.0 (optimum, ph 8.0) and with 0.5-9.0% nacl (optimum, 1.0-5.0%). phylogenetic analyses based on 16s rrna gene sequences showed that strain spio-19(t ... | 2011 | 21421929 |
| biotransformation of bioactive (-)-mellein by a marine isolate of bacterium stappia sp. | the biological transformation of the bioactive dihydroisocoumarin, (-)-mellein, isolated from the marine-derived fungus cladosporium sp., was studied. the preparative-scale culture of (-)-mellein with a marine isolate of a bacterium stappia sp. resulted in the isolation of its oxidized metabolite, (3r,4s)-4-hydroxymellein. the stereostructure of the metabolite obtained was assigned on the basis of detailed physicochemical data analyses. | 2010 | 20622496 |
| nesiotobacter exalbescens gen. nov., sp. nov., a moderately thermophilic alphaproteobacterium from an hawaiian hypersaline lake. | a gram-negative bacterium, designated la33b(t), was isolated from water collected from a hypersaline lake on uninhabited laysan atoll in the northwestern hawaiian islands. cells of strain la33b(t) are motile, straight rods that grow between 4 and 45 degrees c and in media containing 1-17.5% (w/v) nacl. the strain oxidizes carbohydrates, nucleosides, amino acids and organic acids presented as sole carbon sources and constitutive lipolytic and proteolytic enzymes are expressed. over 75% of the fat ... | 2006 | 16514028 |
| stappia aquimarina sp. nov., isolated from seawater. | | 2011 | 21742859 |