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bioaccumulation of cadmium in an experimental aquatic food chain involving phytoplankton (chlorella vulgaris), zooplankton (moina macrocopa), and the predatory catfish clarias macrocephalus x c. gariepinus.the accumulation of cadmium (cd) was studied in an experimental aquatic food chain involving the phytoplankton chlorella vulgaris as the primary producer, the zooplankton moina macrocopa as the primary consumer, and the catfish clarias macrocephalus x clarias gariepinus as the secondary consumer. c. vulgaris was first exposed to cd solutions at 0.00, 0.35, and 3.50 mg l(-1), referred to as control group and experimental groups 1 and 2, respectively. subsequently, each group was fed to three corr ...200616504313
derivation of guideline values for gold (iii) ion toxicity limits to protect aquatic ecosystems.this study focused on estimating the toxicity values of various aquatic organisms exposed to gold (iii) ion (au(3+)), and to propose maximum guideline values for au(3+) toxicity that protect the aquatic ecosystem. a comparative assessment of methods developed in australia and new zealand versus the european community (ec) was conducted. the test species used in this study included two bacteria (escherichia coli and bacillus subtilis), one alga (pseudokirchneriella subcapitata), one euglena (eugl ...201424094731
ecotoxicological assessment of cimetidine and determination of its potential for endocrine disruption using three test organisms: daphnia magna, moina macrocopa, and danio rerio.cimetidine is a histamine h2-receptor antagonist used for treatment of gastrointestinal disorders. it is often detected in aquatic environments, but its ecotoxicological effects have not been well studied. thus, ecotoxicity of cimetidine was evaluated using daphnia magna and moina macrocopa, and zebrafish (danio rerio), and a predicted no effect concentration (pnec) was derived. in d. magna, 48 h immobilization ec50 was determined at 394.9 mg l(-1). however, reproduction damages in d. magna were ...201525957140
evidence of three-level trophic transfer of quantum dots in an aquatic food chain by using bioimaging.in this study, we demonstrated the three-level trophic transfer of quantum dots (qds) within the aquatic food chain. using bioimaging, we observed qd transfer from protozoa (astasia longa) to zooplankton (moina macrocopa) to fish (danio rerio). bioimaging is an effective tool that can improve our understanding of the delivery of nanomaterials in vivo. measurement with an intravital multiphoton laser scanning microscope visually proved the transfer of qds from the first to the second and the seco ...201525119416
toxicity and endocrine disruption in zebrafish (danio rerio) and two freshwater invertebrates (daphnia magna and moina macrocopa) after chronic exposure to mefenamic acid.pharmaceuticals have been frequently detected in the aquatic environment. their potential effects on the endocrine system in wildlife are of special concern because these alterations could lead to impaired reproduction. we evaluated ecotoxicities associated with long-term exposure to mefenamic acid (mfa) and potential endocrine disruption. for this purpose, acute and chronic toxicities of mfa on several aquatic organisms, including two cladocerans, daphnia magna and moina macrocopa, and a teleos ...201323725676
occurrences and ecological risks of roxithromycin, trimethoprim, and chloramphenicol in the han river, korea.we evaluated the occurrence of three antibiotics (roxithromycin, trimethoprim, and chloramphenicol) in surface water and effluents from several sewage treatment plants (stps) on the han river, korea. in addition, standard acute toxicity tests were conducted using the microbe vibrio fischeri, freshwater macroinvertebrates daphnia magna and moina macrocopa, and fish (oryzias latipes) for these antibiotics. antibiotics were more frequently detected at higher amounts in effluent samples and in sampl ...200817944547
cysteine and serine protease-mediated proteolysis in body homogenate of a zooplankter, moina macrocopa, is inhibited by the toxic cyanobacterium, microcystis aeruginosa pcc7806.the paper describes the characterization of proteases in the whole body homogenate of moina macrocopa, which can possibly be inhibited by the extracts of microcystis aeruginosa pcc7806. with the use of oligopeptide substrates and specific inhibitors, we detected the activities of trypsin, chymotrypsin, elastase and cysteine protease. cysteine protease, the predominant enzyme behind proteolysis of a natural substrate, casein, was partially purified by gel filtration. the substrate sds-polyacrylam ...200515820132
potential risks of effluent from acid mine drainage treatment plants at abandoned coal mines.the lethal and sublethal toxicity of effluent from three acid mine drainage treatment plants were monitored from august 2009 to april 2010 using daphnia magna (reference species) and moina macrocopa (indigenous species). acute lethal toxicity was observed in samma effluent due to incomplete neutralization of acid mine drainages by the successive alkalinity producing system (saps). additionally, there was no significant difference in toxicity values (tu) between d. magna and m. macrocopa (p < 0.0 ...201222415647
effect of chronic exposure to acetaminophen and lincomycin on japanese medaka (oryzias latipes) and freshwater cladocerans daphnia magna and moina macrocopa, and potential mechanisms of endocrine disruption.chronic toxicity of acetaminophen and lincomycin were evaluated using freshwater organisms including two crustaceans (daphnia magna and moina macrocopa) and a fish (oryzias latipes). h295r, a human adrenal cell was also used to understand the effects on steroidogenesis. in 21 d d. magna exposure, survival noec was found at 5.72 mg l(-1) and no reproduction related effects were noted at this level of exposure to acetaminophen, while 21 d survival or growth effects were not observed even at the hi ...201222560975
risk assessment of chlortetracycline, oxytetracycline, sulfamethazine, sulfathiazole, and erythromycin in aquatic environment: are the current environmental concentrations safe?to understand potential risks of major pharmaceutical residues in waters, we evaluated ecotoxicities of five major veterinary pharmaceuticals, i.e., chlortetracycline, oxytetracycline, sulfamethazine, sulfathiazole, and erythromycin, which have been frequently detected in freshwater environment worldwide. we conducted acute and chronic toxicity tests using two freshwater invertebrates (daphnia magna and moina macrocopa) and a fish (oryzias latipes). in general, d. magna exhibited greater sensiti ...201222711548
molecular cloning of doublesex genes of four cladocera (water flea) species.the gene doublesex (dsx) is known as a key factor regulating genetic sex determination in many organisms. we previously identified two dsx genes (dapmadsx1 and dapmadsx2) from a freshwater branchiopod crustacean, daphnia magna, which are expressed in males but not in females. d. magna produces males by parthenogenesis in response to environmental cues (environmental sex determination) and we showed that dapmadsx1 expression during embryonic stages is responsible for the male trait development. t ...201323575357
non-monotonic concentration-response relationship of tio(2) nanoparticles in freshwater cladocerans under environmentally relevant uv-a light.the effects of uv-a on the toxicity of tio2 nano-particles (nps) were evaluated using moina macrocopa and daphnia magna under environmentally relevant level of uv-a. the waterfleas were exposed to tio2 nps with different sizes of ~298nm, ~132nm, or ~72nm for up to 48h, with or without uv-a light. whole body reactive oxygen species and transcription of antioxidant enzyme genes were measured, as well as the survival of the waterflea. in the presence of uv-a, the survival rates of m. macrocopa sign ...201424507152
effect of chronic exposure to two components of tritan copolyester on daphnia magna, moina macrocopa, and oryzias latipes, and potential mechanisms of endocrine disruption using h295r cells.tritan copolyester is a novel plastic form from eastman company utilizing three main monomers, 1,4-cyclohexanedimethanol (chdm), dimethyl terephthalate (dmt), and 2,2,4,4-tetramethyl-1,3-cyclobutanediol. despite tritan has been widely applied for plastic bottles, the effects of long-term exposure to these compounds have seldom been investigated. we investigated chronic effects and endocrine disruption potential of chdm and terephthalic acid (tpa), main mammalian metabolite formed from dmt, using ...201526289545
toxic effect of parathion on moina macrocopa metabolism. 20071932864
leaf litter leachates have the potential to increase lifespan, body size, and offspring numbers in a clone of moina macrocopa.leaf litter processing is one major pathway of the global organic carbon cycle. during this process, a variety of small reactive organic compounds are released and transported to the aquatic environment, and may directly impact aquatic organisms as natural xenobiotics. we hypothesize that different forest stockings produce different leachate qualities, which in turn, stress the aquatic communities and, eventually, separate sensitive from tolerant species. particularly, leachates from coniferous ...201122115468
genotoxicity and endocrine disruption potentials of sediment near an oil spill site: 2 years after the hebei spirit oil spill.the hebei spirit oil spill episode (december 7th 2007) has affected the western coastal area of south korea, however, there is limited information on the potential toxicity of oil spill to the ecosystem or humans. the potential toxicity of sediments collected from the affected area (n=22) 2 years after the spill were evaluated. acute lethal toxicity tests using vibrio fischeri and moina macrocopa, and tests for genotoxicity and alteration of steroidogenesis using chicken dt40 cells and h295r cel ...201121786741
[changes in the behaviour of moina macrocopa (crustacea: cladocera) under the influence of gurleya sp. (microsporidia: gurleyidae)].it is established, that infestation with gurleya sp. (microsporidia, gurleyidae) changes the behaviour of its host moina macrocopa (straus, 1820), a crustacean inhabiting pools. heavily infested host individuals attach to surface film of water before their death by senescence. as a consequence, their dead bodies filled with spores of the parasite adhere to objects dipped in water that probably facilitates the transfer of spores by birds and cattle to other pools. weakly infested or uninfested in ...201021309152
hazard assessment of commonly used agricultural antibiotics on aquatic ecosystems.in this study, eleven commonly used antibiotics including sulfonamides, tetracyclines, aminoglycosides, fluoroquinolones, and beta-lactams were evaluated for their acute and chronic aquatic toxicities using standard test organisms e.g., vibrio fischeri, daphnia magna, moina macrocopa, and oryzias latipes. among the antibiotics tested for acute toxicity, neomycin was most toxic followed by trimethoprim, sulfamethoxazole and enrofloxacin. sulfamethazine, oxytetracycline, chlortetracycline, sulfadi ...200818449638
microcystin production by microcystis aeruginosa exposed to different stages of herbivorous zooplankton.microcystin (mc) production by four monoclonal microcystis aeruginosa strains was evaluated in response to infochemicals (indirect exposure) released from different stages of herbivorous zooplankton (neonate/juvenile and adult daphnia magna and moina macrocopa). the intracellular mc and extracellular mc concentrations were significantly different among the control and treatments with zooplankton culture media filtrates (p<0.05), and in most cases mc production was significantly higher (p<0.05) i ...200818243271
the ability of selected cladoceran species to utilize domestic wastewaters in mexico city.the population growth patterns of four cladocerans, viz. alona rectangula, ceriodaphnia dubia, moina macrocopa and daphnia pulex on wastewaters from a treatment plant at iztacalco, mexico city were analyzed in this study. crude wastewater (tank a) did not support a. rectangula and all animals in the replicates died after 5 days. a. rectangula with partially treated wastewater (tank b) showed growth curves similar to controls where algal density was 1 x 10(6) cells ml(-1). with wastewater from ta ...200415084360
exploring embryonic germ line development in the water flea, daphnia magna, by zinc-finger-containing vasa as a marker.vasa is an atp-dependent rna helicase belonging to the dead-box family that, in many organisms, is specifically expressed in germ line cells throughout the life cycle, making it a powerful molecular marker to study germ line development. to obtain further information on germ line development in crustaceans, we cloned vasa cdnas from three branchiopod species: water fleas daphnia magna and moina macrocopa, and brine shrimp artemia franciscana. rna helicase domains in branchiopod vasa were highly ...200515939379
review of recent ecotoxicological studies on cladocerans.cladocerans have been widely used as the bioassay organisms in evaluating the impact of different toxic substances. literature survey during the last 10 years revealed that cladoceran ecotoxicological research has been in an exponential phase constituting nearly 10% of publications on this group. many studies have considered typically planktonic taxa such as daphnia magna, d. pulex, moina macrocopa, m. micrura and ceriodaphania dubia. experimental data on toxicity tests, to a lesser extent, are ...200617090502
the combined effects of heavy metals (copper and zinc), temperature and food (chlorella vulgaris) level on the demographic characters of moina macrocopa (crustacea: cladocera).in this work we evaluated the effect of temperature (22 degrees and 27 degrees c), algal density (chlorella vulgaris 0.5 x 10(6) and 2.0 x 10(6) cells/ml) and different combinations of 2 heavy metals (zn at 25.25-101.0 microg/l and cu at 17.75-71.0 microg/l) on the population level variables of moina macrocopa. median lethal concentration (24 h bioassay at 1 x 10(6) cells/ml of algal diet) of zn and cu for m. macrocopa were 1010 microg/l and 710 microg/l, respectively. in the survivorship curves ...200717680482
toxicity of perfluorooctane sulfonic acid and perfluorooctanoic acid on freshwater macroinvertebrates (daphnia magna and moina macrocopa) and fish (oryzias latipes).because of their global distribution, persistence, and tendency to bioaccumulate, concerns about perfluorooctane sulfonic acid (pfos) and perfluorooctanoic acid (pfoa) are growing. we determined the toxicity of pfos and pfoa in several freshwater organisms, including two cladocerans, daphnia magna and moina macrocopa, and the teleost oryzias latipes. in general, pfos is approximately 10 times more toxic than pfoa in these organisms. in m. macrocopa, the median lethal concentration (lc50) was 17. ...200818593212
long-term evaluation of lethal and sublethal toxicity of industrial effluents using daphnia magna and moina macrocopa.acute toxicity and feeding rate inhibition of effluent from a wastewater treatment plant and its adjacent stream water on daphnia magna and moina macrocopa were comparatively studied. the acute toxicity of the final effluent (fe) fluctuated greatly over the sampling period from january to august 2009. toxicity identification results of the fe in july 2009 showed that cu originating from the fenton's reagent was likely a key toxicant. in addition, the feeding rate of both species was still inhibi ...201020211525
endocrine disruption and consequences of chronic exposure to ibuprofen in japanese medaka (oryzias latipes) and freshwater cladocerans daphnia magna and moina macrocopa.despite frequent detection of ibuprofen in aquatic environments, the hazards associated with long-term exposure to ibuprofen have seldom been investigated. ibuprofen is suspected of influencing sex steroid hormones through steroidogenic pathways in both vertebrates and invertebrates. in this study, the effect of ibuprofen on sex hormone balance and the associated mechanisms was investigated in vitro by use of h295r cells. we also conducted chronic toxicity tests using freshwater fish, oryzias la ...201020236711
chronic exposure to diclofenac on two freshwater cladocerans and japanese medaka.consequences of long-term exposure to diclofenac up to 3 months were evaluated using freshwater crustaceans (daphnia magna and moina macrocopa) and a fish (oryzias latipes). marked decrease of reproduction was observed at 25 mg/l for d. magna, and at 50 mg/l for m. macrocopa. three-month exposure of fish to 0.001-10 mg/l of diclofenac resulted in significant decreasing trend in hatching success and delay in hatch. the hatching of the eggs produced from the fish exposed to 10 mg/l was completely ...201121489627
optimal conditions for three brood chronic toxicity test method using a freshwater macroinvertebrate moina macrocopa.freshwater cladocera such as daphnia magna and ceriodaphnia dubia have been used extensively for freshwater toxicity test worldwide. however, these species may not be indigenous in certain geographical regions, which restrict the utility of these organisms as test species. in the present study, we investigated optimal culture and test conditions for an indigenous freshwater macroinvertebrate of korea, moina macrocopa. the culture conditions that were evaluated included water temperature (20°c an ...201221769559
dissolved humic substances initiate dna-methylation in cladocerans.dna-methylation is one pathway of epigenetic programming of gene expression and can be responsive to environmental challenges such as methylating agents in the food. here we report on the dna-methylation in the cladocerans daphnia magna and moina macrocopa exposed to humic substances, ubiquitous biogeochemicals. the methylation of dna can alter the stress response, presumably including exposure to synthetic xenobiotic chemicals.201121963594
purification and properties of blood hemoglobin from the fresh-water cladocera, moina macrocopa and daphnia magna. 19715553981
ecological risk assessment of the antibiotic enrofloxacin applied to pangasius catfish farms in the mekong delta, vietnam.antibiotics applied in aquaculture production may be released into the environment and contribute to the deterioration of surrounding aquatic ecosystems. in the present study, we assessed the ecological risks posed by the use of the antibiotic enrofloxacin (enr), and its main metabolite ciprofloxacin (cip), in a pangasius catfish farm in the mekong delta region, vietnam. water and sediment samples were collected in a stream receiving effluents from a pangasius catfish farm that had applied enr. ...201525063964
identification of multi-level toxicity of liquid crystal display wastewater toward daphnia magna and moina macrocopa.toxicity-based regulations of industrial effluent have been adopted to complement the conventional discharge limits based on chemical analyses. in this study, multi-level toxicity including acute toxicity, feeding rate inhibition and oxidative stress of effluent from a liquid crystal display (lcd) wastewater treatment plant (wwtp) to daphnia magna (reference species) and moina macrocopa (native species) were periodically monitored from april 2010 to april 2011. raw wastewater was acutely toxic t ...201222677053
toxicity of reactive red 141 and basic red 14 to algae and waterfleas.textile wastewater normally has a visible color although it has low concentration. this may affect the aquatic ecosystem. two dyestuffs, reactive red 141 (rr141) and basic red14 (br14) were used as compound models. rr 141 is an anionic dye which has a big molecule whereas br 14 is a cationic dye and has a small molecule. the target organisms for toxicity test were green algae (chlorella sp.) and waterfleas (moina macrocopa). the effect of humic acid on the toxicity of dyestuffs to test organisms ...200818845856
[the artificial infection of the cladoceran moina macrocopa (crustacea: phyllopoda) with the microsporidia gurleya sp. (microsporidia: gurleyidae)].microcrustaceans moina macrocopa (straus, 1820) have been collected in a small vernal pond and cultured under laboratory conditions. the infection of this culture with the microsporidia gurleya sp. was detected and high virulence of this microsporidian species allowed us to carry out the transmission experiments. horizontal transmission, the high host specificity and maximal spore production in the hypodermal and fat body cells through 5-6 days after per oral experimental infection have been rev ...200717144406
viral haemorrhagic septicaemia virus (vhsv) remains viable for several days but at low levels in the water flea moina macrocopa.viral haemorrhagic septicaemia virus (vhsv) genotype ivb has been isolated from amphipods belonging to the genus diporeia, but it has yet to be established whether crustacean zooplankton act as vectors of this virus for fish species. therefore, we evaluated the viability of infectious vhsv in the water flea moina macrocopa. vhsv was re-isolated from replicate groups of m. macrocopa that had been immersed with 108.0, 107.0, and 105.0 tcid50 ml-1 of vhsv (dk-3592b, genotype ia). furthermore, 40 m. ...201729256423
production of male neonates in four cladoceran species exposed to a juvenile hormone analog, fenoxycarb.previous studies have found that exposure of a cyclic parthenogen, the water flea daphnia magna (cladocera, crustacea), to juvenile hormones and their analogs results in the production of neonates of male sex at concentration-dependent rates. we conducted reproduction experiments in four different species (moina macrocopa, m. micrura, ceriodaphnia dubia and c. reticulata) of cladoceran to test for the first time whether the occurrence of this phenomenon after exposure of the parent to such hormo ...200515910904
a freshwater shrimp (paratya compressa improvisa) as a sensitive test organism to pesticides.the susceptibility of 2-week-old individuals of the freshwater shrimp, paratya compressa improvisa, to five kinds of insecticide and five kinds of herbicide was examined in comparison with that of two species of cladocera, daphnia magna and moina macrocopa. the shrimp was especially sensitive to two organophosphorus insecticides. the 48-h lc50 values for fenitrothion and fenthion to the shrimp were 1.15 and 1.04 microg litre(-1) (mean value, n=2), in contrast with 37.8 and 35.3 microg litre(-1) ...198915092400
elaphoidella grandidieri (harpacticoida: copepoda): demographic characteristics and possible use as live prey in aquaculture.in freshwater ecosystems, rotifers and cladocerans are ideal prey for fish larvae whereas copepods, due to their purported low growth rate and predatory tendency, are not. we recently isolated the parthenogenetic elaphoidella grandidieri (gueme et richard, 1893) a benthic freshwater harpacticoid, from a fish farm in the state of morelos, central mexico and tested its potential as a live prey organism for larval vertebrates. population growth and life table demography experiments were conducted, ...201122315830
correlation of the five test methods to assess chemical toxicity and relation to physical properties.biological tests using orizias latipes (lc50 and oxygen uptake test), moina macrocopa (lc50), and dugesia japonica (head regeneration test and lc50) were carried out in order to clarify the mutual relationship of these test methods. the oxygen uptake rate of o. latipes was not effective to assess chemical toxicity. adding the results of the growth inhibition test of tetrahymena pyriformis (yoshioka, y., ose, y., and sato, t. (1985). sci. total environ. 43, 149-157), the correlation coefficients ...19863093184
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