microbial diversity of mid-stage palinurid phyllosoma from great barrier reef waters. | this study aimed to determine the bacterial community associated with wild-caught, mid-stage larvae of spiny lobsters (palinuridae) in their native oligotrophic marine environment, and to compare their diversity and composition with communities associated with aquaculture-reared larvae of the tropical rock lobster panulirus ornatus. | 2008 | 18298531 |
screening of marine bacteria with bacteriocin-like activities and probiotic potential for ornate spiny lobster (panulirus ornatus) juveniles. | bacteriocins are ribosomally synthesized antimicrobial peptides, which have been found in diverse bacterial species of terrestrial origins and some from the sea. new bacteriocins with new characteristics, new origins and new applications are likely still awaiting discovery. the present study screened bacteria isolated from marine animals of interest to the aquaculture industry for antimicrobial and bacteriocin-like activities in order to uncover biodiversity of bacteriocin producers, and explore ... | 2014 | 24969424 |
identification of an antagonistic probiotic combination protecting ornate spiny lobster (panulirus ornatus) larvae against vibrio owensii infection. | vibrio owensii dy05 is a serious pathogen causing epizootics in the larviculture of ornate spiny lobster panulirus ornatus. in the present study a multi-tiered probiotic screening strategy was used to identify a probiotic combination capable of protecting p. ornatus larvae (phyllosomas) from experimental v. owensii dy05 infection. from a pool of more than 500 marine bacterial isolates, 91 showed definitive in vitro antagonistic activity towards the pathogen. antagonistic candidates were shortlis ... | 2012 | 22792184 |
probiont niche specialization contributes to additive protection against vibrio owensii in spiny lobster larvae. | the development of efficient probiotic application protocols for use in marine larviculture relies on comprehensive understanding of pathogen-probiont-host interactions. the probiont combination of pseudoalteromonas sp. pp107 and vibrio sp. pp05 provides additive protection against vectored vibrio owensii dy05 infection in larvae (phyllosomas) of ornate spiny lobster, panulirus ornatus. here, fluorescently tagged strains were used to demonstrate niche specialization of these probionts in both th ... | 2012 | 23757129 |
effects of mannan oligosaccharide dietary supplementation on performances of the tropical spiny lobsters juvenile (panulirus ornatus, fabricius 1798). | the effects of dietary mannan oligosaccharide (mos) (bio-mos, alltech, usa) on the growth, survival, physiology, bacteria and morphology of the gut and immune response to bacterial infection of tropical rock lobsters (panulirus ornatus) juvenile were investigated. dietary inclusion level of mos at 0.4% was tested against the control diet (trash fish) without mos inclusion. at the end of 56 days of rearing period, a challenged test was also conducted to evaluate the bacterial infection resistant ... | 2010 | 20034574 |
vibrio owensii sp. nov., isolated from cultured crustaceans in australia. | two bacterial strains (dy05(t) and 47666-1) were isolated in queensland, australia, from diseased cultured crustaceans panulirus ornatus and penaeus monodon, respectively. on the basis of 16s rrna gene sequence identity, the strains were shown to belong to the harveyi clade of the genus vibrio. multilocus sequence analysis using five housekeeping genes (rpoa, pyrh, topa, ftsz and mreb) showed that the strains form a monophyletic group with 94.4% concatenated sequence identity to the closest spec ... | 2010 | 20002183 |
isolation of lytic bacteriophage against vibrio harveyi. | the isolation of lytic bacteriophage of vibrio harveyi with potential for phage therapy of bacterial pathogens of phyllosoma larvae from the tropical rock lobster panulirus ornatus. | 2010 | 19886890 |
microbial diversity within early-stage cultured panulirus ornatus phyllosomas. | a thorough understanding of the microorganisms and pathogens associated with the larval stage of the tropical ornate rock lobster, panulirus ornatus, is required to overcome disease outbreaks that currently block aquaculture attempts. this study used microscopy in addition to culture and molecularly based microbiological techniques to characterize the bacterial community associated with cultured, developmental stage pi to pii p. ornatus phyllosomas. scanning electron microscopy demonstrated colo ... | 2007 | 17220250 |
experimental transmission and tissue tropism of white spot syndrome virus (wssv) in two species of lobsters, panulirus homarus and panulirus ornatus. | the susceptibility of two species of lobsters, panulirus homarus and panulirus ornatus to white spot syndrome virus (wssv) was tested by oral route and intramuscular injection. the results revealed that these lobsters were as highly susceptible as marine shrimp when the wssv was administered intramuscularly. the wssv caused 100% mortality in both panulirus homarus and panulirus ornatus, at 168 and 120 h, respectively, after intramuscular injection and failed to cause mortality when given orally. ... | 2006 | 16905144 |
novel polymorphic microsatellite markers for panulirus ornatus and their cross-species primer amplification in panulirus homarus. | polymorphic microsatellite loci were isolated for panulirus ornatus using 454 gs-flx titanium pyrosequencing. fifteen markers containing perfect di-, tri-, tetra-, and penta-nucleotide motifs were consistently co-amplified in five multiplexes in a panel of 91 randomly selected samples. observed number of alleles varied from 2 to 14 per locus. observed and expected heterozygosity ranged from 0.090 to 0.79 and 0.08 to 0.87, respectively. ten loci deviated from hardy-weinberg equilibrium after sequ ... | 2016 | 27565876 |
pathogenicity and infection cycle of vibrio owensii in larviculture of the ornate spiny lobster (panulirus ornatus). | the type strain of vibrio owensii (dy05) was isolated during an epizootic of aquaculture-reared larvae (phyllosomas) of the ornate spiny lobster (panulirus ornatus). v. owensii dy05 was formally demonstrated to be the etiological agent of a disease causing rapid and reproducible larval mortality with pathologies similar to those seen during disease epizootics. vectored challenge via the aquaculture live feed organism artemia (brine shrimp) caused consistent cumulative mortality rates of 84 to 89 ... | 2012 | 22307306 |