| staphylococcus fleurettii sp. nov., isolated from goat's milk cheeses. | a new coagulase-negative and novobiocin-resistant species of the genus staphylococcus, staphylococcus fleurettii, isolated from raw-milk cheeses, is described. this species is differentiated from the other novobiocin-resistant staphylococci on the basis of ribotype and intergenic transcribed spacer patterns, dna-dna reassociation reactions, cell wall composition and phenotypic characteristics. s. fleurettii could be distinguished by its oxidase activity, by its ability to produce acid aerobicall ... | 2000 | 10939659 |
| supplementary biochemical tests useful for the differentiation of oxidase positive staphylococci. | differentiation of the oxidase positive staphylococci, staphylococcus sciuri, staphylococcus lentus, staphylococcus vitulinus and staphylococcus fleurettii, based on tributyrin, urease, caseinase, gelatinase and dnase activity is described. these tests may be used for preliminary identification of oxidase positive isolates of staphylococci resulting in more accurate identification of these species. | 2007 | 17207599 |
| evolutionary origin of the staphylococcal cassette chromosome mec (sccmec). | several lines of evidence indicate that the most primitive staphylococcal species, the staphylococcus sciuri group, were involved in the first stages of evolution of sccmec - the genetic element carrying the β-lactam resistance gene meca. however, many steps are still missing from this evolutionary history. in particular, it is not known how meca was incorporated into the mobile element scc prior to dissemination among staphylococcus aureus and other pathogenic staphylococcal species.to gain ins ... | 2017 | 28373201 |
| characterization of staphylococci in urban wastewater treatment plants in spain, with detection of methicillin resistant staphylococcus aureus st398. | the objective of this study was to determine the prevalence of staphylococcus in urban wastewater treatment plants (uwtp) of la rioja (spain), and to characterize de obtained isolates. 16 wastewater samples (8 influent, 8 effluent) of six uwtps were seeded on mannitol-salt-agar and oxacillin-resistance-screening-agar-base for staphylococci and methicillin-resistant staphylococcus aureus recovery. antimicrobial susceptibility profile was determined for 16 antibiotics and the presence of 35 antimi ... | 2016 | 26840519 |
| interaction between bovine-associated coagulase-negative staphylococci species and strains and bovine mammary epithelial cells reflects differences in ecology and epidemiological behavior. | bacteria adherence seems to be an essential first stage for the internalization of bacteria into the cytoplasm of the host cell, which is considered an important virulence strategy enabling bacteria to occupy a microenvironment separated from host defense mechanisms. thus, this study aimed to explore the difference in the capacity of 4 bovine-associated staphylococci species or strains to adhere to and internalize into bovine mammary epithelial cells (mec). three different isolates of coagulase- ... | 2016 | 26830736 |
| the new macrolide-lincosamide-streptogramin b resistance gene erm(45) is located within a genomic island in staphylococcus fleurettii. | genome alignment of a macrolide, lincosamide, and streptogramin b (mlsb)-resistant staphylococcus fleurettii strain with an mlsb-susceptible s. fleurettii strain revealed a novel 11,513-bp genomic island carrying the new erythromycin resistance methylase gene erm(45). this gene was shown to confer inducible mlsb resistance when cloned into staphylococcus aureus. the erm(45)-containing island was integrated into the housekeeping gene guaa in s. fleurettii and was able to form a circular intermedi ... | 2015 | 25779586 |
| technical note: a pilot study using a mouse mastitis model to study differences between bovine associated coagulase-negative staphylococci. | coagulase-negative staphylococci (cns) are a group of bacteria classified as either minor mastitis pathogens or commensal microbiota. recent research suggests species- and even strain-related epidemiological and genetic differences within the large cns group. the current pilot study investigated in 2 experiments whether a mouse mastitis model validated for bovine staphylococcus aureus can be used to explore further differences between cns species and strains. in a first dose titration experiment ... | 2015 | 25497801 |
| the ecological importance of the staphylococcus sciuri species group as a reservoir for resistance and virulence genes. | the staphylococcus sciuri species group includes five species that are most often presented as commensal animal-associated bacteria. the species of this group are staphylococcus sciuri (with three subspecies), staphylococcus lentus, staphylococcus vitulinus, staphylococcus fleurettii and staphylococcus stepanovicii. members of these group are commonly found in a broad range of habitats including animals, humans and the environment. however, those species have been isolated also from infections, ... | 2014 | 24629775 |
| characterization of methicillin-resistant non-staphylococcus aureus staphylococci carriage isolates from different bovine populations. | this study aimed at investigating bovine non-staphylococcus aureus staphylococci for their role as a potential reservoir for methicillin resistance. | 2013 | 23090977 |
| draft genome sequence of staphylococcus fleurettii strain mbts-1 isolated from cucumber. | the draft genome of staphylococcus fleurettii mbts-1, isolated from cucumber in northern germany, was sequenced. analysis showed that the assembled genome had a size of 2,582,128 bp with a predicted total of 2,491 protein-encoding genes, 9 rrnas, 5 ncrnas, and 44 trnas. this strain did not contain plasmid dna. | 2017 | 28495782 |
| genetic characterization of antimicrobial resistance in coagulase-negative staphylococci from bovine mastitis milk. | coagulase-negative staphylococci (cns; n=417) were isolated from bovine milk and identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. nineteen different species were identified, and staphylococcus xylosus, staphylococcus chromogenes, staphylococcus haemolyticus, and staphylococcus sciuri were the most prevalent species. resistance to oxacillin (47.0% of the isolates), fusidic acid (33.8%), tiamulin (31.9%), penicillin (23.3%), tetracycline (15.8%), streptom ... | 2013 | 23415536 |
| milk prolactin response and quarter milk yield after experimental infection with coagulase-negative staphylococci in dairy heifers. | coagulase-negative staphylococci (cns) are the most common bacteria involved in subclinical mastitis in dairy cows. remarkably, cns-infected dairy heifers produce more milk than uninfected heifers. because the lactation hormone prolactin (prl) is also involved in mammary gland immunity, we investigated the milk prl response and the mammary quarter milk yield following experimental cns challenge. eight healthy holstein-friesian heifers in mid-lactation were experimentally infected using a split-u ... | 2015 | 25981074 |
| local host response following an intramammary challenge with staphylococcus fleurettii and different strains of staphylococcus chromogenes in dairy heifers. | coagulase-negative staphylococci (cns) are a common cause of subclinical mastitis in dairy cattle. the cns inhabit various ecological habitats, ranging between the environment and the host. in order to obtain a better insight into the host response, an experimental infection was carried out in eight healthy heifers in mid-lactation with three different cns strains: a staphylococcus fleurettii strain originating from sawdust bedding, an intramammary staphylococcus chromogenes strain originating f ... | 2016 | 27176792 |
| staphylococcus stepanovicii sp. nov., a novel novobiocin-resistant oxidase-positive staphylococcal species isolated from wild small mammals. | five novobiocin-resistant and oxidase-positive staphylococcal strains were isolated from wild small mammals. phenotypic studies and phylogenetic analysis based on 16s rrna, rpob and dnaj gene sequences revealed that these strains were members of the staphylococcus sciuri cluster group and were similar to staphylococcus fleurettii. dna-dna hybridisation with closely related staphylococcal species suggested that the strains represented a novel species. the name staphylococcus stepanovicii is propo ... | 2010 | 20418037 |