| massilia timonae gen. nov., sp. nov., isolated from blood of an immunocompromised patient with cerebellar lesions. | a fastidious, slowly growing, strictly aerobic, gram-negative bacterium was isolated from a culture of blood from a 25-year-old man with common variable immunodeficiency. the man had been admitted to hospital with febrile progressive cerebellar ataxia. the use of standard phenotypic schemes did not lead to identification, but sequence analysis demonstrated that the 16s rrna gene of the isolate was most similar to those of the environmental bacteria duganella zoogloeoides (formerly zoogloea ramig ... | 1998 | 9738031 |
| molecular analysis of the trimethylamine n-oxide (tmao) reductase respiratory system from a shewanella species. | trimethylamine n-oxide (tmao) is an abundant compound of tissues of marine fish and invertebrates. during fish spoilage, certain marine bacteria can reduce tmao to nauseous trimethylamine (tma). one such bacterium has been isolated and identified as a new shewanella species, and called shewanella massilia. the anaerobic growth of s. massilia is greatly increased when tmao is added, indicating that tmao reduction involves a respiratory pathway. the tora enzyme responsible for tmao reduction is a ... | 1998 | 9813127 |
| crystal structure of oxidized trimethylamine n-oxide reductase from shewanella massilia at 2.5 a resolution. | the periplasmic trimethylamine n-oxide (tmao) reductase from the marine bacteria shewanella massilia is involved in a respiratory chain, having trimethylamine n-oxide as terminal electron acceptor. this molybdoenzyme belongs to the dimethyl sulfoxide (dmso) reductase family, but has a different substrate specificity than its homologous enzyme. while the dmso reductases reduce a broad spectra of organic s-oxide and n-oxide compounds, tmao reductase from shewanella massilia reduces only tmao as th ... | 1998 | 9813128 |
| characterization and multiple molecular forms of tord from shewanella massilia, the putative chaperone of the molybdoenzyme tora. | several bacteria use trimethylamine n-oxyde (tmao) as an exogenous electron acceptor for anaerobic respiration. this metabolic pathway involves expression of the tor operon that codes for a periplasmic molybdopterin-containing reductase of the dmso/tmao family, a pentahemic c-type cytochrome, and the tord cytoplasmic chaperone, possibly required for acquisition of the molybdenum cofactor and translocation of the reductase by the twin-arginine translocation system. in this report, we show that th ... | 2002 | 12192070 |
| characteristics of massilia timonae and massilia timonae-like isolates from human patients, with an emended description of the species. | the description of massilia timonae, a nonfermentative aerobic gram-negative rod, was based on a single strain. a subsequent report of a second isolate has been recently published. phenotypic descriptions of these two strains were based primarily on commercial test kit results. we have identified three additional strains as m. timonae by 16s rrna sequence analysis and have characterized them phenotypically in parallel with the type strain of m. timonae, cip 105350, by conventional test methods. ... | 2003 | 12517847 |
| a novel protein fold and extreme domain swapping in the dimeric tord chaperone from shewanella massilia. | tord is the cytoplasmic chaperone involved in the maturation of the molybdoenzyme tora prior to the translocation of the folded protein into the periplasm. the x-ray structure at 2.4 a resolution of the tord dimer reveals extreme domain swapping between the two subunits. the all-helical architecture of the globular domains within the intertwined molecular dimer shows no similarity with known protein structures. according to sequence similarities, this new fold probably represents the architectur ... | 2003 | 12575936 |
| use of a packed-column bioreactor for isolation of diverse protease-producing bacteria from antarctic soil. | seventy-five aerobic heterotrophs have been isolated from a packed-column bioreactor inoculated with soil from antarctica. the column was maintained at 10 degrees c and continuously fed with a casein-containing medium to enrich protease producers. twenty-eight isolates were selected for further characterization on the basis of morphology and production of clearing zones on skim milk plates. phenotypic tests indicated that the strains were mainly psychrotrophs and presented a high morphological a ... | 2003 | 12620829 |
| respiration of 13c-labeled substrates added to soil in the field and subsequent 16s rrna gene analysis of 13c-labeled soil dna. | our goal was to develop a field soil biodegradation assay using (13)c-labeled compounds and identify the active microorganisms by analyzing 16s rrna genes in soil-derived (13)c-labeled dna. our biodegradation approach sought to minimize microbiological artifacts caused by physical and/or nutritional disturbance of soil associated with sampling and laboratory incubation. the new field-based assay involved the release of (13)c-labeled compounds (glucose, phenol, caffeine, and naphthalene) to soil ... | 2003 | 12620850 |
| temporal change in culturable phenanthrene degraders in response to long-term exposure to phenanthrene in a soil column system. | widespread environmental contamination by polycyclic aromatic hydrocarbons (pah) has led to increased interest in the use of natural attenuation as a clean-up strategy. however, few bioremediation studies have investigated the behaviour of the indigenous pah-degrading community after long-term exposure to a pah. in this study, a column packed with sandy loam soil was exposed to a solution saturated with phenanthrene ( approximately 1.2 mg l-1) for a 6-month period to examine the temporal respons ... | 2003 | 14510842 |
| studies on the interaction of nadph with rhodobacter sphaeroides biotin sulfoxide reductase. | rhodobacter sphaeroides biotin sulfoxide reductase (bsor) contains the bis(molybdopterin guanine dinucleotide)molybdenum cofactor and catalyzes the reduction of d-biotin-d-sulfoxide to biotin. this protein is the only member of the dimethyl sulfoxide reductase family of molybdopterin enzymes that utilizes nadph as the direct electron donor to the catalytic mo center. kinetic studies using stopped-flow spectrophotometry indicate that bsor reduction by nadph (>1000 s(-1)) is faster than steady-sta ... | 2004 | 15366932 |
| diversity of n-acyl homoserine lactone-producing and -degrading bacteria in soil and tobacco rhizosphere. | in gram-negative bacteria, quorum-sensing (qs) communication is mostly mediated by n-acyl homoserine lactones (n-ahsl). the diversity of bacterial populations that produce or inactivate the n-ahsl signal in soil and tobacco rhizosphere was investigated by restriction fragment length polymorphism (rflp) analysis of amplified 16s dna and dna sequencing. such analysis indicated the occurrence of n-ahsl-producing strains among the alpha-, beta- and gamma-proteobacteria, including genera known to pro ... | 2005 | 16232294 |
| massilia dura sp. nov., massilia albidiflava sp. nov., massilia plicata sp. nov. and massilia lutea sp. nov., isolated from soils in china. | four gram-negative, motile, rod-shaped bacterial strains were isolated from soil samples collected from south-east china. a taxonomic study including phylogenetic analysis based on 16s rrna gene sequences and phenotypic characteristics was performed. dna g+c contents of the four strains were 63-66 mol%. their predominant ubiquinone was q-8. the fatty acid profiles contained c16:1omega7c (36.9-54.7%) and c16:0 (22.8-25.5%) as the major components. based on their phenotypic characteristics, phylog ... | 2006 | 16449458 |
| massilia aurea sp. nov., isolated from drinking water. | a gram-negative, motile, rod-shaped organism, strain ap13(t), able to produce yellow-pigmented colonies, was isolated from the drinking water distribution system of seville (spain) and was characterized by using a polyphasic taxonomic approach. in 16s rrna gene sequence comparisons, strain ap13(t) exhibited 96.9-95.6 % similarity with respect to the five recognized species of the genus massilia. the dna g+c content of strain ap13(t) was 66.0 mol%, a value that supports the affiliation of strain ... | 2006 | 17012578 |
| undibacterium pigrum gen. nov., sp. nov., isolated from drinking water. | two gram-negative, rod-shaped, oxidase-positive, non-spore-forming, non-motile bacteria (strains ccug 49009(t) and ccug 49012), both isolated from drinking water, were characterized. on the basis of chemotaxonomic data [major ubiquinone, q-8; predominant polyamines, putrescine and 2-hydroxyputrescine; major polar lipids, phosphatidylethanolamine, moderate amounts of diphosphatidylglycerol and phosphatidylglycerol and minor amounts of three aminolipids and phosphatidylserine; major fatty acids, c ... | 2007 | 17625185 |
| structure of the molybdenum site of escherichia coli trimethylamine n-oxide reductase. | we report a structural characterization of the molybdenum site of recombinant escherichia coli trimethylamine n-oxide (tmao) reductase using x-ray absorption spectroscopy. the enzyme active site shows considerable similarity to that of dimethyl sulfoxide (dmso) reductase, in that, like dmso reductase, the tmao reductase active site can exist in multiple forms. examination of the published crystal structure of tmao oxidase from shewanella massilia indicates that the postulated mo coordination str ... | 2008 | 18163615 |
| massilia brevitalea sp. nov., a novel betaproteobacterium isolated from lysimeter soil. | a gram-negative, strictly aerobic bacterium, designated strain byr23-80t, was isolated from lysimeter soil by using a high-throughput cultivation technique. cells of strain byr23-80t were found to be short rods that multiplied by binary fission and were motile by means of a single polar flagellum. occasionally, two to three polar or lateral flagella were observed. the optimum growth temperature was 15 degrees c and the ph optimum was 7.0-7.5. the predominant cellular fatty acids were c16 : 1omeg ... | 2008 | 18450722 |
| massilia aerilata sp. nov., isolated from an air sample. | a novel aerobic, gram-negative, rod-shaped, motile bacterium, designated strain 5516s-11(t), was isolated from air samples collected in the suwon region of the republic of korea. analysis of the 16s rrna gene sequence indicated that the organism belongs to the genus massilia; the highest sequence similarity (97.2 %) was found with respect to massilia aurea dsm 18055(t). cells of strain 5516s-11(t) contained ubiquinone q-8 as the predominant isoprenoid quinone and possessed summed feature 3 (c(16 ... | 2008 | 18523189 |
| interactions between strains of 2,4-diacetylphloroglucinol-producing pseudomonas fluorescens in the rhizosphere of wheat. | abstract strains of fluorescent pseudomonas spp. that produce the antibiotic 2,4-diacetylphoroglucinol (2,4-dapg) are among the most effective rhizobacteria controlling diseases caused by soilborne pathogens. the genotypic diversity that exists among 2,4-dapg producers can be exploited to improve rhizosphere competence and biocontrol activity. knowing that d-genotype 2,4-dapg-producing strains are enriched in some take-all decline soils and that p. fluorescens q8r1-96, a representative d-genotyp ... | 2003 | 18943865 |
| massilia virus, a novel phlebovirus (bunyaviridae) isolated from sandflies in the mediterranean. | a new virus was isolated from three independent pools of phlebotomus perniciosus sandflies (diptera; psychodidae) trapped in two regions of southeastern france, located 90 miles apart. microscopic, antigenic and genetic analyses indicate that this novel virus belongs to the genus phlebovirus in the family bunyaviridae. the new virus is designated massilia virus since the first isolate was obtained from sandflies collected in the suburban area of marseille. the complete genome sequence was determ ... | 2009 | 19055373 |
| massilia niabensis sp. nov. and massilia niastensis sp. nov., isolated from air samples. | two bacterial isolates, designated strains 5420s-26(t) and 5516s-1(t), were recovered from air samples collected in suwon, korea. cells of both strains were aerobic, gram-negative, motile rods. phylogenetically, these strains were positioned within the radius of the genus massilia. 16s rrna gene sequence analysis showed that the strains shared 97.3 % sequence similarity and had sequence similarities of 94.9-98.1 % with respect to type strains of species belonging to the genus massilia. in dna-dn ... | 2009 | 19542125 |
| bacterial communities in pah contaminated soils at an electronic-waste processing center in china. | surface soils from guiyu, china (an intense e-waste processing center) were analyzed for persistent organic pollutants (pops) and variations in composition of the resident bacterial communities. denaturing gradient gel electrophoresis analysis of bacterial 16s rrna gene showed that e-waste pollution altered the bacterial community structure by promoting changes in species composition and species richness. bacterial diversity was not decreased at e-waste open-burning sites, compared with a non e- ... | 2010 | 19633954 |
| massilia jejuensis sp. nov. and naxibacter suwonensis sp. nov., isolated from air samples. | two gram-negative, motile, rod-shaped bacteria (strains 5317j-18t and 5414s-25t) were isolated from air samples collected in the jeju island and suwon region of korea, respectively. phylogenetically, strain 5317j-18t was grouped with the genus massilia with massilia brevitalea byr23-80t as the closest relative (98.8% sequence similarity). strain 5414s-25t was affiliated with the genus naxibacter with naxibacter haematophilus ccug 38318t as the closest relative (98.8% sequence similarity). the me ... | 2010 | 19783608 |
| purification and characterization of two extracellular endochitinases from massilia timonae. | two extracellular chitinases (designated as chi-56 and chi-64) produced by massilia timonae were purified by ion-exchange chromatography, ammonium sulfate precipitation, and gel-filtration chromatography. the molecular mass of chi-56 was 56 kda as determined by both sds-page and gel-filtration chromatography. on the other hand, chi-64 showed a molecular mass of 64 kda by sds-page and 28 kda by gel-filtration chromatography suggesting that its properties may be different from those of chi-56. the ... | 2010 | 19962135 |
| hit-and-run stimulation: a novel concept to reactivate latent hiv-1 infection without cytokine gene induction. | current antiretroviral therapy (art) efficiently controls hiv-1 replication but fails to eradicate the virus. even after years of successful art, hiv-1 can conceal itself in a latent state in long-lived cd4(+) memory t cells. from this latent reservoir, hiv-1 rebounds during treatment interruptions. attempts to therapeutically eradicate this viral reservoir have yielded disappointing results. a major problem with previously utilized activating agents is that at the concentrations required for ef ... | 2010 | 20538859 |
| a revision of massilia la scola et al. 2000, with an emended description of the genus, and the inclusion of all species of the genus naxibacter as new combinations and a proposal of massilia consociata sp. nov. | a gram-negative, rod-shaped, non-spore-forming bacterium originating from a human clinical specimen was studied for its taxonomic position. 16s rrna gene sequence similarity studies clearly allocated this strain (ccug 58010t) to the betaproteobacteria, closely related to members of both genera massilia and naxibacter. naxibacter varians was shown to be the most closely related species on the basis of 16s rrna gene sequence similarity (97,5%), followed by massilia niastensis (96.8%) and massilia ... | 2010 | 20656816 |
| granada virus: a natural phlebovirus reassortant of the sandfly fever naples serocomplex with low seroprevalence in humans. | a new member of the phlebovirus genus, tentatively named granada virus, was detected in sandflies collected in spain. by showing the presence of specific neutralizing antibodies in human serum collected in granada, we show that granada virus infects humans. the analysis of the complete genome of granada virus revealed that this agent is likely to be a natural reassortant of the recently described massilia virus (donor of the long and short segments) with a yet unidentified phlebovirus (donor of ... | 2010 | 20889862 |
| responses of the bacterial community to chronic gamma radiation in a rice paddy ecosystem. | purpose: public concern about radiation protection of the environment and non-human species is increasing. the potential impact of chronic gamma radiation on the bacterial community in a rice paddy ecosystem was investigated. materials and methods: paddy field microcosm samples were continuously exposed at a dose rate of 1 gy day(-1) for five days after which the bacterial community structures in them were compared with those of unirradiated control samples by denaturing gradient gel electrophor ... | 2011 | 21284570 |
| bacterial communities in aerosols and manure samples from two different dairies in central and sonoma valleys of california. | aerosols have been suspected to transport food pathogens and contaminate fruits and vegetables grown in close proximity to concentrated animal feeding operations, but studies are lacking that substantiate such transport. to monitor the potential transport of bacteria originated from fresh or dry manure through aerosols on a dairy, we identified by 16s rrna sequencing, bacteria in aerosols collected within 2 to 3 meters from dairy cows at two dairies. gram-positive firmicutes were predominant in ... | 2011 | 21364996 |
| massilia oculi sp. nov., isolated from a human clinical specimen. | a gram-negative, rod-shaped, non-spore-forming bacterium isolated from a patient suffering from an endophthalmitis was studied for its taxonomic position. 16s rrna gene sequence similarity studies clearly allocated this strain (ccug 43427a(t)) to the genus massilia. massilia timonae was shown to be the most closely related species on the basis of 16s rrna gene sequence similarity (97.4%), followed by massilia aurea (97.2%). similarities to all other massilia species were below 97.0%. chemotaxono ... | 2011 | 21441374 |
| massilia flava sp. nov., isolated from a soil sample. | a gram-negative-staining, rod-shaped bacterium, designated strain y9t, was isolated from a soil sample collected in ningxia province in china and characterized in order to determine its taxonomic position. strain y9t contained q-8 as the predominant ubiquinone. major fatty acid components were summed feature 3 (comprising c16:1ω7c and/or iso-c15:0 2-oh) and c16:0. the major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. the g+c content of genomic dna ... | 2011 | 21515701 |
| massilia timonae infection presenting as generalized lymphadenopathy in a man returning from nigeria. | we report a case of apparent malaria infection presented with a syndrome of painless, generalized lymphadenopathy without granulomas shortly after exposure to fresh water in rural west africa. residual infection with massilia timonae was diagnosed and successfully treated with cotrimoxazole. | 2011 | 21525215 |
| molecular phylogeny of the genus dactylopius (hemiptera: dactylopiidae) and identification of the symbiotic bacteria. | phylogenetic analyses, from polymerase chain reaction (pcr)-amplified 12s rrna and 18s rrna gene sequences from cochineal insects of the genus dactylopius present in mexico, showed that d. ceylonicus, d. confusus, and d. opuntiae are closely related. d. coccus constitutes a separate clade, and d. tomentosus is the most distantly related. bacterial 16s rrna sequences from all the dactylopius species sampled showed a common ß-proteobacteria, related to azoarcus, also found in eggs and in bacterio ... | 2010 | 22127169 |
| Bacteria attachment to surfaces--AFM force spectroscopy and physicochemical analyses. | Understanding bacterial adhesion to surfaces requires knowledge of the forces that govern bacterial-surface interactions. Biofilm formation on stainless steel 316 (SS316) by three bacterial species was investigated by examining surface force interaction between the cells and metal surface using atomic force microscopy (AFM). Bacterial-metal adhesion force was quantified at different surface delay time from 0 to 60s using AFM tip coated with three different bacterial species: Gram-negative Massil ... | 2011 | 21889162 |
| Massilia tieshanensis sp. nov., isolated from Tieshan mining soil, China. | A bacterial isolate, designated strain TS3T, was isolated from soil collected from a metal mine in Tieshan District, Daye City, Hubei Province, central China. Cells of this strain were Gram-negative, motile and rod-shaped. The strain had ubiquinone Q-8 as the predominant respiratory quinone, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol as the major polar lipids and summed feature 3 (C16:1 ?7c and/or iso-C15:0 2-OH), C16:0 and C18:1 ?7c as the major fatty acids. The G ... | 2011 | 22140160 |
| massilia sp. bs-1, a novel violacein-producing bacterium isolated from soil. | a novel bacterium, massilia sp. bs-1, producing violacein and deoxyviolacein was isolated from a soil sample collected from akita prefecture, japan. the 16s ribosomal dna of strain bs-1 displayed 93% homology with its nearest violacein-producing neighbor, janthinobacterium lividum. strain bs-1 grew well in a synthetic medium, but required both l-tryptophan and a small amount of l-histidine to produce violacein. | 2011 | 21979084 |
| plant original massilia isolates producing polyhydroxybutyrate, including one exhibiting high yields from glycerol. | aims: the purpose of this study was to isolate new and potentially better polyhydroxyalkanoate (pha) producing bacteria, with a view to obtaining high yields from inexpensive substrates like glycerol, a major by-product of the biodiesel process. methods and results: 11 new plant original isolates of the genus massilia, a poorly studied lineage within the betaproteobacteria, were isolated and characterized. two isolates, 2c4 and 4d3c, could not be assigned to a validated massilia species and pr ... | 2011 | 22212185 |