| burkholderia tuberum sp. nov. and burkholderia phymatum sp. nov., nodulate the roots of tropical legumes. | the taxonomic status of five root nodule isolates from tropical legumes was determined using a polyphasic taxonomic approach. two isolates were identified as b. caribensis, an organism originally isolated from soil in martinique (the french west indies). one isolate was identified as burkholderia cepacia genomovar vi, a b. cepacia complex genomovar thus far only isolated from sputum of cystic fibrosis patients. the remaining two isolates were identified as novel burkholderia species for which we ... | 2002 | 12583710 |
| legume symbiotic nitrogen fixation by beta-proteobacteria is widespread in nature. | following the initial discovery of two legume-nodulating burkholderia strains (l. moulin, a. munive, b. dreyfus, and c. boivin-masson, nature 411:948-950, 2001), we identified as nitrogen-fixing legume symbionts at least 50 different strains of burkholderia caribensis and ralstonia taiwanensis, all belonging to the beta-subclass of proteobacteria, thus extending the phylogenetic diversity of the rhizobia. r. taiwanensis was found to represent 93% of the mimosa isolates in taiwan, indicating that ... | 2003 | 14645288 |
| proof that burkholderia strains form effective symbioses with legumes: a study of novel mimosa-nodulating strains from south america. | twenty mimosa-nodulating bacterial strains from brazil and venezuela, together with eight reference mimosa-nodulating rhizobial strains and two other beta-rhizobial strains, were examined by amplified rrna gene restriction analysis. they fell into 16 patterns and formed a single cluster together with the known beta-rhizobia, burkholderia caribensis, burkholderia phymatum, and burkholderia tuberum. the 16s rrna gene sequences of 15 of the 20 strains were determined, and all were shown to belong t ... | 2005 | 16269788 |
| burkholderia terrae sp. nov., isolated from a forest soil. | a gram-negative, slightly curved rod-shaped bacterium, designated strain kmy02t, was isolated from a forest soil in daejeon, south korea. on the basis of 16s rrna gene sequence similarity, strain kmy02t was shown to belong to the family burkholderiaceae of the betaproteobacteria, and to be related most closely to burkholderia hospita lmg 20598t (98.7%), burkholderia caribensis lmg 18531t (98.0%) and burkholderia phymatum lmg 21445t (97.4%). its phylogenetic distance from all recognized species w ... | 2006 | 16449457 |
| burkholderia phymatum is a highly effective nitrogen-fixing symbiont of mimosa spp. and fixes nitrogen ex planta. | * the ability of burkholderia phymatum stm815 to effectively nodulate mimosa spp., and to fix nitrogen ex planta, was compared with that of the known mimosa symbiont cupriavidus taiwanensis lmg19424. * both strains were equally effective symbionts of m. pudica, but nodules formed by stm815 had greater nitrogenase activity. stm815 was shown to have a broader host range across the genus mimosa than lmg19424, nodulating 30 out of 31 species, 21 of these effectively. lmg19424 effectively nodulated o ... | 2007 | 17176403 |
| mixotrophic metabolism in burkholderia kururiensis subsp. thiooxydans subsp. nov., a facultative chemolithoautotrophic thiosulfate oxidizing bacterium isolated from rhizosphere soil and proposal for classification of the type strain of burkholderia kururiensis as burkholderia kururiensis subsp. kururiensis subsp. nov. | a thiosulfate-oxidizing facultative chemolithoautotrophic burkholderia sp. strain atsb13(t) was previously isolated from rhizosphere soil of tobacco plant. strain atsb13(t) was aerobic, gram-staining-negative, rod shaped and motile by means of sub-terminal flagellum. strain atsb13(t) exhibited mixotrophic growth in a medium containing thiosulfate plus acetate. a phylogenetic study based on 16s rrna gene sequence analysis indicated that strain atsb13(t) was most closely related to burkholderia ku ... | 2009 | 19841903 |
| phylogenetic relationships and diversity of β-rhizobia associated with mimosa species grown in sishuangbanna, china. | in order to investigate the genetic diversity of rhizobia associated with various exotic and invasive species in tropical mainland china, 116 bacterial isolates were obtained from mimosa root nodules collected from sishuangbanna and yuanjiang districts of yunnan province. isolated rhizobia were characterized by rflp analysis of 16s rrna genes, sds-page of whole-cell proteins and box-pcr. most of the isolated strains were identified as β-rhizobia belonging to diverse populations of burkholderia a ... | 2011 | 20228206 |
| nodulation and nitrogen fixation by mimosa spp. in the cerrado and caatinga biomes of brazil. | *an extensive survey of nodulation in the legume genus mimosa was undertaken in two major biomes in brazil, the cerrado and the caatinga, in both of which there are high degrees of endemicity of the genus. *nodules were collected from 67 of the 70 mimosa spp. found. thirteen of the species were newly reported as nodulating. nodules were examined by light and electron microscopy, and all except for m. gatesiae had a structure typical of effective mimosa nodules. the endosymbiotic bacteria in nodu ... | 2010 | 20456044 |
| burkholderia phymatum strains capable of nodulating phaseolus vulgaris are present in moroccan soils. | phylogenetic analysis of 16s rrna, nodc, and nifh genes of four bacterial strains isolated from root nodules of phaseolus vulgaris grown in morocco soils were identified as burkholderia phymatum. all four strains formed n(2)-fixing nodules on p. vulgaris and mimosa, acacia, and prosopis species and reduced acetylene to ethylene when cultured ex planta. | 2010 | 20472732 |
| genetic diversity of mimosa pudica rhizobial symbionts in soils of french guiana: investigating the origin and diversity of burkholderia phymatum and other beta-rhizobia. | the genetic diversity of 221 mimosa pudica bacterial symbionts trapped from eight soils from diverse environments in french guiana was assessed by 16s rrna pcr-rflp, rep-pcr fingerprints, as well as by phylogenies of their 16s rrna and reca housekeeping genes, and by their nifh, noda and nodc symbiotic genes. interestingly, we found a large diversity of beta-rhizobia, with burkholderia phymatum and burkholderia tuberum being the most frequent and diverse symbiotic species. other species were als ... | 2011 | 22093060 |