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genetic analysis of avian influenza a viruses isolated from domestic waterfowl in live-bird markets of hanoi, vietnam, preceding fatal h5n1 human infections in 2004.the first known cases of human infection with highly pathogenic avian influenza (hpai) h5n1 viruses in vietnam occurred in late 2003. however, hpai h5n1 and low-pathogenic avian influenza (lpai) h5n2 and h9n3 viruses were isolated from domestic waterfowl during live-bird market (lbm) surveillance in vietnam in 2001 and 2003. to understand the possible role of these early viruses in the genesis of h5n1 strains infecting people, we performed sequencing and molecular characterization. phylogenetic ...200919578928
evaluation of neuraminidase antigen based competitive enzyme-linked immunosorbent assay in chickens vaccinated with avian influenza inactivated vaccine.a competitive enzyme-linked immunosorbent assay (c-elisa) was developed as a serologic diagnostic tool to detect antibodies against na subtype 3 of avian influenza virus (aiv). the na antigen used in this c-elisa was obtained by pronase treatment of allantoic fluid of specific-pathogen-free (spf) eggs infected with aiv. the na specific monoclonal antibodies were produced from purified na. the n3 c-elisa was carried out on serum samples collected from both spf chickens and commercial layers to co ...201020521715
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