| differentiation of potyviruses and their strains by hybridization with the 3' non-coding region of the viral genome. | nucleic acid hybridization with the 3' non-coding region of the potyvirus genome as the probe was shown to be a relatively simple means of distinguishing between distinct potyviruses and their strains. comparisons of the nucleotide sequences of potyvirus genomes (ignoring gaps) showed that the degree of identity between equivalent genes of strains was greater than 96%, while between distinct potyviruses the identity ranged from 42% to 65%, suggesting that any extended sequence could be considere ... | 1992 | 1551936 |
| coat protein of potyviruses. 7. amino acid sequence of peanut stripe virus. | the amino acid sequence of the 287-residue coat protein of peanut stripe virus (pstv) was determined from the sequences of overlapping peptide fragments. results indicated that the amino terminus was blocked by an acetyl group, as has previously been found for the coat protein of johnsongrass mosaic potyvirus. comparison of the pstv sequence with coat proteins of 20 distinct potyviruses gave sequence identities of 47-57%, except for zucchini yellow mosaic virus (zymv), passionfruit woodiness vir ... | 1991 | 1863222 |
| nucleotide sequences of the coat protein genes of two aphid-transmissible strains of soybean mosaic virus. | the nucleotide sequences of the coat protein genes and 3' non-coding regions of two aphid-transmissible isolates (g2 and g7) of soybean mosaic virus (smv) were determined. the coat protein of the g2 isolate differs from that of the aphid non-transmissible n isolate by a single amino acid at position 12 (aspartic acid in n, glycine in g2 and g7). the g7 isolate differs from g2 and n at three and four amino acid residues, respectively. the nucleotide sequence similarity of the three isolates in th ... | 1991 | 2016589 |
| isolation and serological comparison of virus-coded proteins of three potyviruses infecting cucurbitaceous plants. | cylindrical inclusion proteins (cips), amorphous inclusion proteins (aips), and virus particles were partially purified from cucurbita maxima leaves infected with zucchini yellow mosaic virus (zymv), watermelon mosaic virus 2 (wmv2), and the watermelon mosaic virus 1 strain of papaya ringspot virus (prsv-w). antisera to these individual antigens, except aips of zymv and wmv2, were prepared and used to determine with electroblot-elisa the serological differentiation indices (sdis) between homolog ... | 1990 | 2338401 |
| watermelon mosaic virus ii and zucchini yellow mosaic virus: cloning of 3'-terminal regions, nucleotide sequences, and phylogenetic comparisons. | the 3'-terminal genomic regions of an isolate of watermelon mosaic virus ii (wmvii) and a florida isolate of zucchini yellow mosaic virus (zymv-f) have been cloned. the nucleotide sequence of the wmvii cdna clone shows the presence of the large nuclear inclusion protein gene, the coat protein gene and 3' untranslated region. the nucleotide sequence of a zymv-f cdna clone shows the presence of the coat protein gene and 3' untranslated region. comparisons of the nucleotide and deduced amino acid s ... | 1990 | 2374006 |
| cdna cloning and sequence analysis of the 3'-terminal region of zucchini yellow mosaic virus rna. | the 3' half of the rna of the cucurbit potyvirus zucchini yellow mosaic virus (zymv) was genetically cloned and the cdna sequence of a portion of the putative rna polymerase gene, the complete coat protein gene and the 3' untranslated region was determined. the coat protein and putative polymerase genes are both part of a continuous open reading frame as is the case for other potyviruses whose genomes are expressed as polyproteins. the q/s protease cleavage site for the n terminus of the coat pr ... | 1990 | 2374012 |
| coat protein of potyviruses. 6. amino acid sequences suggest watermelon mosaic virus 2 and soybean mosaic virus-n are strains of the same potyvirus. | the amino acid sequence of the coat protein of watermelon mosaic virus 2 (wmv 2) was determined by a combination of peptide and nucleic acid sequencing. the coat protein of wmv 2 contained 281 amino acid residues including a single cysteine at position 132 and a blocked amino terminus. comparison with the coat protein sequences of 20 strains of ten distinct potyviruses showed sequence homologies ranging from 43% to 69% except for the n strain of soybean mosaic virus (smv-n), where the sequence h ... | 1989 | 2719555 |
| the use of 3' non-coding nucleotide sequences in the taxonomy of potyviruses: application to watermelon mosaic virus 2 and soybean mosaic virus-n. | the sequence of the 3' 1106 nucleotides of the watermelon mosaic virus 2 (wmv 2) genome has been determined. the sequence contains the complete coding region of the viral coat protein followed by a 3' untranslated sequence of 251 nucleotides. when these sequences were compared with the equivalent regions of the n strain of soybean mosaic virus (smv-n), the coat protein coding regions were 82% homologous, whereas the 3' untranslated sequences were 78% homologous. optimal alignment of the 3' untra ... | 1989 | 2794980 |
| complementary dna cloning and expression of the papaya ringspot potyvirus sequences encoding capsid protein and a nuclear inclusion-like protein in escherichia coli. | three cdna clones that express viral gene products in escherichia coli jm83 were derived from a watermelon mosaic virus-1 strain of papaya ringspot virus (prsv-w). dnas complementary to portions of the viral rna were inserted into the puc8 and puc9 plasmids, and the expressed polypeptides were fusion products with the amino terminus of beta-galactosidase. clones w1-77 and w2-1 expressed fusion products with apparent molecular weights of 40,000 (40k) and 14k, respectively, which were serologicall ... | 1985 | 2998020 |
| the inhibitory activity of some plant juices on the infectivity of watermelon mosaic virus. | | 1969 | 4389116 |
| [effect of watermelon mosaic virus on the development of cucurbitaceae crops]. | | 1972 | 4663645 |
| wind effects on spread of watermelon mosaic virus 1 from local virus sources to watermelon. | | 1974 | 4839221 |
| aggregated filaments in extracts from lupinus infected with watermelon mosaic virus. | | 1968 | 5662872 |
| disruption of watermelon mosaic virus-induced inclusions by phosphotungstate. | | 1968 | 5723677 |
| [demonstration of the watermelon mosaic virus in imported watermelons and their possible significance as virus sources]. | | 1965 | 5898365 |
| watermelon mosaic virus-morocco is a distinct potyvirus. | the relationship of the morocco isolate of watermelon mosaic virus (wmv) to wmv2, soybean mosaic virus (a virus closely related to wmv2) and the w strain of papaya ringspot virus (prsv-w), formerly wmv1, was examined by comparing tryptic peptide profiles using high performance liquid chromatography. the profiles indicated that the coat protein sequence of wmv-morocco differed substantially from those of the other potyviruses. this conclusion was supported by sequence data from five tryptic pepti ... | 1993 | 8347085 |
| nucleotide sequence, serology and symptomatology suggest that vanilla necrosis potyvirus is a strain of watermelon mosaic virus ii. | vanilla necrosis potyvirus (vnv) is the cause of significant losses to the south pacific islands vanilla crop. the gene for the coat protein of vnv has been cloned and sequenced. comparison of this gene with other potyviral coat protein sequences revealed 97% nucleotide sequence homology (98% amino acid homology) to a us isolate of watermelon mosaic virus ii (wmv-ii), 93% nucleotide sequence homology (96% amino acid homology) to an australian isolate of wmv-ii and 81% nucleotide sequence homolog ... | 1993 | 8470960 |
| identification of zucchini yellow mosaic potyvirus by rt-pcr and analysis of sequence variability. | a reverse transcription-polymerase chain reaction (rt-pcr) method was used to identify zucchini yellow mosaic virus (zymv) in leaves of infected cucurbits. oligonucleotide primers which annealed to regions in the nuclear inclusion body (nib) and the coat protein (cp) genes, generated a 300-bp product from zymv and also from the closely related watermelon mosaic virus type 2 (wmv-2). however, no product was obtained from papaya ringspot potyvirus which also infects cucurbits. zymv and wmv-2 were ... | 1995 | 8576311 |
| properties of a virus causing mosaic and leaf curl disease of celosia argentea l. in nigeria. | a sap transmissible virus, causing mosaic and leaf curl disease of celosia argentea, was isolated at vegetable farms in amuwo odofin, tejuoso, and abule ado, lagos, nigeria. the virus had a restricted host range confined to a few species of the amaranthaceae, chenopodiaceae and solanaceae families. it failed to infect several other species of the aizoaceae, brassicaceae, cucurbitaceae, fabaceae, lamiaceae, malvaceae, poaceae and tiliaceae families. the virus was transmitted in a non-persistent m ... | 1998 | 9842442 |
| affinity purification of hc-pro of potyviruses with ni2+-nta resin. | the hc-pro of zucchini yellow mosiac virus (zymv) was found to bind to ni2+-nta resin with or without his-tagging. the binding stringency was similar to that observed in proteins with a zinc finger motif like the hc-pro. using this characteristic we developed an efficient and rapid method (2-3 h) for purification of the hc-pro of several potyviruses. a dominant protein of about 150 kda was extracted and identified as the hc-pro of zymv by means of immunoblotting. about 150 microg of hc-pro were ... | 1998 | 9923736 |
| molecular characterization and coat protein serology of watermelon leaf mottle virus (potyvirus). | a cdna library was generated from purified rna of watermelon leaf mottle virus (wlmv) (genus potyvirus). two overlapping clones totaling 2,316 nucleotides at the 3' terminus of the virus were identified by immunoscreening with coat protein antiserum. the sequence analyses of the clones indicated an open reading frame (orf) of 2,050 nucleotides which encoded part of the replicase and the coat protein, a 243-nucleotide non-coding region (3'utr), and 23 adenine residues of the poly (a) tail. the ta ... | 2000 | 10795529 |
| ribozyme genes protecting transgenic melon plants against potyviruses. | potyviruses are the most important viral pathogens of crops worldwide. under a contract with gene shears pty limited, we are using ribozyme genes to protect melon plants against two potyviruses: wmv2 and zymv. different polyribozyme genes were designed, built and introduced into melons plants. transgenic melon plants containing a resistance gene were obtained and their progeny was challenged by the appropriate virus. most of the genes tested conferred some degree of resistance to the viruses in ... | 2001 | 11471972 |
| on the evolution and molecular epidemiology of the potyvirus papaya ringspot virus. | the potyvirus papaya ringspot virus (prsv) is found throughout the tropics and subtropics. its p biotype is a devastating pathogen of papaya crops and its w biotype of cucurbits. prsv-p is thought to arise by mutation from prsv-w. however, the relative impact of mutation and movement on the structure of prsv populations is not well characterized. to investigate this, we have determined the coat protein sequences of isolates of both biotypes of prsv from vietnam (50), thailand (13), india (1) and ... | 2002 | 12237441 |
| zone electrophoresis and electron microscopy of a watermelon mosaic virus from south africa. | | 1960 | 14436981 |
| a virus related to soybean mosaic virus from pinellia ternata in china and its comparison with local soybean smv isolates. | a potyvirus isolated from pinellia ternata in china was characterised and shown to be related to soybean mosaic virus (smv). the virus was pathogenic on p. ternata and some soybean cultivars, whereas the local soybean smv isolate hh5 did not infect p. ternata. western blot experiments demonstrated a serological relationship between the virus from pinellia, smv and watermelon mosaic virus (wmv). the complete nucleotide sequences of the pinellia virus (isolate p-1, 9735 nt) and of the chinese soyb ... | 2004 | 14745600 |
| variability and genetic structure of the population of watermelon mosaic virus infecting melon in spain. | the genetic structure of the population of watermelon mosaic virus (wmv) in spain was analysed by the biological and molecular characterisation of isolates sampled from its main host plant, melon. the population was a highly homogeneous one, built of a single pathotype, and comprising isolates closely related genetically. there was indication of temporal replacement of genotypes, but not of spatial structure of the population. analyses of nucleotide sequences in three genomic regions, that is, i ... | 2004 | 14972570 |
| the nucleotide sequence of watermelon mosaic virus (wmv, potyvirus) reveals interspecific recombination between two related potyviruses in the 5' part of the genome. | watermelon mosaic virus (wmv, potyvirus) is a potyvirus with a worldwide distribution, mostly in temperate and mediterranean regions. according to the partial sequences that were available, wmv appeared to share high sequence similarity with soybean mosaic virus (smv), and it was almost considered as a strain of smv in spite of its different and much broader host range. like smv, it was also related to legume-infecting potyviruses belonging to the " bean common mosaic virus (bcmv) subgroup". in ... | 2004 | 15290385 |
| inheritance of resistance to zucchini yellow mosaic virus and watermelon mosaic virus in watermelon. | high resistance to zucchini yellow mosaic virus-china strain (zymv-ch) and moderate resistance to watermelon mosaic virus (wmv) were found in a selection of pi 595203 (citrullus lanatus var. lanatus), an egusi type originally collected in nigeria. mixed inoculations showed primarily that these two viruses have no cross-protection. this fact may explain the high frequency of mixed infection often observed in commercial fields. when plants were inoculated with a mixture of the two viruses, the fre ... | 2004 | 15475395 |
| movement of coat protein genes from a commercial virus-resistant transgenic squash into a wild relative. | we monitored pollen-mediated transgene dissemination from commercial transgenic squash czw-3 into its wild relative cucurbita pepo ssp. ovifera var. texana (c. texana). transgenic squash czw-3 expresses the neomycin phosphotransferase ii (nptii) gene and the coat protein (cp) genes of cucumber mosaic virus (cmv), zucchini yellow mosaic virus (zymv), and watermelon mosaic virus (wmv); thereby, it is resistant to these three aphid-borne viruses. the rate of npt ii and cp transgene introgression in ... | 2004 | 15612351 |
| comparative fitness of a wild squash species and three generations of hybrids between wild x virus-resistant transgenic squash. | we compared some fitness components of the wild squash species cucurbita pepo spp. ovifera var. texana (c. texana) and three generations of hybrids (f1, bc1, and bc2) between c. texana and commercial transgenic squash czw-3 over three consecutive years under field conditions of low (ldp) and high disease pressure (hdp) by cucumber mosaic virus (cmv), zucchini yellow mosaic virus (zymv) and watermelon mosaic virus (wmv). transgenic squash czw-3 expresses the coat protein (cp) genes of cmv, zymv, ... | 2004 | 15612352 |
| unusual cytoplasmic inclusions induced by watermelon mosaic virus. | | 1976 | 948866 |
| occurrence, distribution and relative incidence of mosaic viruses infecting field--grown squash in tehran province, iran. | squash (cucurbita pepo) belongs to cucurbitaceae family. every year cucurbitaceae are planted world wide. they are one of the most important economic crops. cucurbitaceae are threatened by viruses. many viruses damage the plants of this family. since nine viruses have been reported on squash from iran. in this survey, during 2002--2003, to determine the distribution of cucumber mosaic virus (cmv), zucchini yellow mosaic virus (zymv) and watermelon mosaic virus (wmv), 466 samples were collected f ... | 2004 | 15756831 |
| the genomic sequence of wisteria vein mosaic virus and its similarities with other potyviruses. | the complete nucleotide sequence of a beijing isolate of wisteria vein mosaic virus was determined to be 9695 nucleotides in length excluding the poly(a) tail. sequence analysis predicted a single large open reading frame of 9279 nucleotides potentially encodes a polyprotein of 3092 amino acids. phylogenetic analysis based on the genomic and deduced amino acid sequences support the current status of wisteria vein mosaic virus (wvmv) as a distinct virus of the genus potyvirus and a member of the ... | 2006 | 16732423 |
| the complete nucleotide sequence of a pakistani isolate of watermelon mosaic virus provides further insights into the taxonomic status in the bean common mosaic virus subgroup. | watermelon mosaic virus (wmv) is a potyvirus with a worldwide distribution, but is mostly found in temperate and mediterranean regions. the complete nucleotide (nt) sequence of a pakistani isolate of wmv (wmv-pk) was determined and compared with french isolate (wmv-fr) and other closely related potyviruses. wmv-pk showed overall identities of 94.4% (nt) and 96% (amino acid; aa) with the wmv-fr. however, variability was observed in the 5' utr and p1 region. although sequence identities over most ... | 2006 | 16732483 |
| comparative spatial spread overtime of zucchini yellow mosaic virus (zymv) and watermelon mosaic virus (wmv) in fields of transgenic squash expressing the coat protein genes of zymv and wmv, and in fields of nontransgenic squash. | the spatial and temporal patterns of aphid-vectored spread of zucchini yellow mosaic virus (zymv) and watermelon mosaic virus (wmv) were monitored over two consecutive years in plantings of nontransgenic and transgenic squash zw-20h (commercial cv. freedom ii) and zw-20b, both expressing the coat protein genes of zymv and wmv. all test plants were surrounded by nontransgenic plants that were mechanically inoculated with zymv or wmv, and served as primary virus source. across all trials, none of ... | 2006 | 16838196 |
| molecular evidence that zucchini yellow fleck virus is a distinct and variable potyvirus related to papaya ringspot virus and moroccan watermelon mosaic virus. | zucchini yellow fleck virus (zyfv, genus potyvirus) infects cultivated or wild cucurbits in the mediterranean basin and occasionally causes severe damage in crops. biological and serological data tend to indicate that zyfv is related to other cucurbit-infecting potyviruses, mainly papaya ringspot virus (prsv) and moroccan watermelon mosaic virus (mwmv). in order to establish unambiguously the taxonomic status of zyfv, the sequence of the 3' part of the genome - encompassing the cp coding region ... | 2007 | 16969629 |
| serological and molecular variability of watermelon mosaic virus (genus potyvirus). | watermelon mosaic virus (wmv, genus potyvirus) is very common in cucurbits worldwide, but its variability has been little studied. in france, where wmv has been known since 1974, unusually severe symptoms on zucchini squash have been found to be associated with wmv since 1999. we have developed serological and molecular tools to study wmv variability and the origin of severe strains. eight monoclonal antibodies were obtained, characterized by epitope mapping, and used to assess the serological v ... | 2007 | 17219020 |
| biological characterization and complete nucleotide sequence of a tunisian isolate of moroccan watermelon mosaic virus. | during a survey conducted in october 2005, cucurbit leaf samples showing virus-like symptoms were collected from the major cucurbit-growing areas in tunisia. das-elisa showed the presence of moroccan watermelon mosaic virus (mwmv, potyvirus), detected for the first time in tunisia, in samples from the region of cap bon (northern tunisia). mwmv isolate tn05-76 (mwmv-tn) was characterized biologically and its full-length genome sequence was established. mwmv-tn was found to have biological propert ... | 2008 | 17978887 |
| molecular characterization and evolutionary analysis of soybean mosaic virus infecting pinellia ternata in china. | twenty-nine pinellia ternata specimens were collected from representative areas in china, including the major production provinces of zhejiang, henan, shanxi, hunan, shandong and hubei. seven isolates related to soybean mosaic virus (smv), which could be pathogenic on p. ternata and some soybean [glycine max (l.) merr.] cultivars, were detected using double antibody sandwich immunosorbent assay (das-elisa) and rt-pcr amplification performed with degenerate primer of potyviruses. it is revealed t ... | 2008 | 18058013 |
| [research on secretion expression in pichia pastoris and function of the hc-pro gene of watermelon mosaic virus]. | hc-pro gene of watermelon mosaic virus was obtained by rt-pcr was 1371bp in length. it was cloned into ppi(9k, then the eucaryotic recombinant expression plasmid ppic9k-whc was constructed. after being linearized with restriction endonuclease sal i , the recombinant plasmid was transformed into pichia pastoris gs115 by electroporation. the high copy transformants with mut+ /his+ phenotype were selected by rt-pcr and screening on g418, md and mm medium. induced by methanol for 5 days, the culture ... | 2007 | 18257244 |
| algerian watermelon mosaic virus (awmv): a new potyvirus species in the prsv cluster. | a potyvirus was isolated from a naturally infected squash plant in algeria in 1986. biological and serological data have revealed that the virus, initially described as h4, is related to other cucurbit-infecting potyviruses, particularly moroccan watermelon mosaic virus (mwmv) and papaya ringspot virus (prsv). to establish unequivocally the taxonomic status of h4, its full-length genome sequence was established. h4 shared identities of 70% and 65% at the amino acid level with mwmv and prsv, resp ... | 2008 | 18484176 |
| occurrence and distribution of ten viruses infecting cucurbit plants in guilan province, iran. | during the 2006 and 2007 growing seasons, a systematic survey was conducted in open-field of melon (cucumis melo l.), cucumber (c. sativus l.), squash (cucurbita sp.), and watermelon (citrulus lanatus l.) crops in 16 major cucurbit-growing areas of guilan province in iran. symptomatic leaf samples were collected and screened by double-antibody sandwich elisa (das-elisa) or rt-pcr to detect zucchini yellow mosaic virus (zymv), watermelon mosaic virus (wmv), cucurbit aphid-borne yellows virus (cab ... | 2008 | 18564898 |
| different helper factors associated with aphid transmission of some potyviruses. | myzus persicae transmitted watermelon mosaic virus (wmv) after acquiring it through artificial membranes from a solution of purified virus mixed with the soluble fraction from infected leaf extracts or by prefeeding on the soluble fraction before acquiring purified virus. wmv-induced helper factor assisted m. persicae in transmitting purified turnip mosaic virus (tumv) but not potato virus y (pvy). tumv-induced helper factor from infected leaves was ineffective for the transmission of purified w ... | 1981 | 18635077 |
| isolation and partial characterization of the amorphous cytoplasmic inclusions associated with infections caused by two potyviruses. | the potyviruses pepper mottle (pemv) and watermelon mosaic virus-1, a strain of papaya ringspot (prsv-w), induce the formation of a second type of cytoplasmic inclusion in addition to cylindrical (pinwheel) inclusions. the conspicuous aggregates of electron-dense material with imperfect spherical shapes have been called amorphous inclusions (ai). the ai were isolated from extracts of infected tissue using a combination of clarification with triton x-100 and low-speed centrifugations through sucr ... | 1985 | 18639842 |
| identification of potyviral amorphous inclusion protein as a nonstructural, virus-specific protein related to helper component. | antisera to amorphous inclusion (ai) proteins associated with infections by pepper mottle virus (pemv) and the watermelon mosaic virus-1 strain of papaya ringspot virus (prsv-w) were used to probe in vitro translation products of the viral rnas. the major translation product of pemv rna in the rabbit reticulocyte lysate (rrl) system was a previously reported polypeptide of apparent molecular weight 78,000 (mr 78k). it reacted with anti-ai serum, whereas the major translation product in the wheat ... | 1985 | 18639843 |
| topographic analysis of tobacco etch virus capsid protein epitopes. | monoclonal antibodies have been prepared, which react with capsid protein of an aphid-transmitted isolate of tobacco etch virus (tev). ten different monoclonal antibodies were characterized with reference to (1) antibody class, (2) reactivity with different plant virus antigens, (3) the spatial relationship between epitopes, and (4) whether these epitopes were located on the exterior surface of the virion. three monoclonal antibodies were specific for tev isolates. these monoclonal antibodies re ... | 1985 | 18640514 |
| evidence for multiple intraspecific recombinants in natural populations of watermelon mosaic virus (wmv, potyvirus). | the full-length sequences of 13 isolates of watermelon mosaic virus (wmv, genus potyvirus) belonging to the three main molecular groups defined at the capsid level were obtained and compared to 4 sequences available in databases. many isolates presented evidence for intraspecific recombination, particularly between molecular groups 1 and 2. most isolates had different recombination breakpoints suggestive of multiple independent recombination events. the breakpoints were for the most part located ... | 2008 | 18668194 |
| watermelon mosaic virus: tubular inclusions in pumpkin leaves and aggregates in leaf extracts. | | 1967 | 6028936 |
| [demonstration of the specific watermelon mosaic virus in cuba]. | | 1966 | 5990844 |
| analysis of the biological and molecular variability of watermelon mosaic virus isolates from iran. | watermelon mosaic virus (wmv) is one of the most important viruses that causes different symptoms in cucurbitaceae. wmv is a potyvirus with a worldwide distribution, but occurs most commonly in temperate and mediterranean regions. cucurbit species grown in yazd, esfahan, west azerbaijan, hormozgan, and kerman provinces were surveyed for the relative incidence of wmv in 2004-2005. a total of 757 symptomatic cucurbit and 31 weed species were collected and assayed for infection with wmv. of 788 lea ... | 2008 | 18712590 |
| inheritance of resistance to watermelon mosaic virus in cucumis melo that impairs virus accumulation, symptom expression, and aphid transmission. | abstract the cucumis melo accession tgr-1551 was found to be resistant to watermelon mosaic virus (wmv, genus potyvirus, family potyviridae). the resistance resulted in a drastic and significant reduction of virus titer and infected plants were asymptomatic or exhibited mild disease symptoms. the same gene or closely linked genes restricted virus accumulation and ameliorated symptom expression. no effect was observed on virus accumulation in inoculated leaves, which suggested that the initial ph ... | 2005 | 18943018 |
| characterization of synergy between cucumber mosaic virus and potyviruses in cucurbit hosts. | abstract mixed infections of cucurbits by cucumber mosaic virus (cmv) and potyviruses exhibit a synergistic interaction. zucchini squash and melon plants coinfected by the potyvirus zucchini yellow mosaic virus (zymv) and either fny-cmv (subgroup ia) or ls-cmv (subgroup ii) displayed strong synergistic pathological responses, eventually progressing to vascular wilt and plant death. accumulation of fny- or ls-cmv rnas in a mixed infection with zymv in zucchini squash was slightly higher than infe ... | 2002 | 18944139 |
| influence of cytoplasmic heat shock protein 70 on viral infection of nicotiana benthamiana. | the accumulation of heat shock protein 70 (hsp70) generally occurs in plants infected with viruses. however, the effect of hsp70 accumulation on plant viral infection and pathogenesis remains elusive. in this study, the expression of six hsp70 genes was found to be induced by the four diverse rna viruses, tobacco mosaic virus, potato virus x (pvx), cucumber mosaic virus and watermelon mosaic virus, in nicotiana benthamiana. heat treatment enhanced the accumulation and systemic infection of these ... | 2008 | 19019009 |
| population size and incidence of virus infection in free-living populations of cucurbita pepo. | impact assessments of virus resistance transgene introgression into wild, free-living populations are important for determining whether these transgenes present a risk to agriculture or the environment. transgenic virus-resistant cucurbita pepo ssp. ovifera var. ovifera l. (squash) cultivars have been commercialized, and may be cultivated in close proximity to cross-compatible wild, free-living relatives (c. pepo subsp. pepo vars. ozarkana and texana). therefore, the potential impact of these vi ... | 2008 | 19081007 |
| emergence of new strains of watermelon mosaic virus in south-eastern france: evidence for limited spread but rapid local population shift. | severe symptoms caused by watermelon mosaic virus (wmv) in zucchini squash leaves and fruits have been observed since 1999 in south-eastern (se) france. their appearance correlates with the introduction of new, "emerging" (em) isolates distant at the molecular level from the "classic" (cl) isolates present for more than 30 years. to understand the origin and spread of em isolates, a survey was performed between 2004 and 2007. wmv isolates collected were characterized by sequencing part of the po ... | 2009 | 19152813 |
| inhibitory effect of watermelon mosaic virus on meloidogyne javanica (treub) chitwood infecting cucurbita pepo l. | | 1984 | 19295884 |
| evaluation and application of a luciferase fusion system for rapid in vivo analysis of rnai targets and constructs in plants. | the practical use of rna-mediated approaches including antisense rna, ribozymes and sirnas for specific inhibition of gene expression is limited by lack of simple quantitative methods to rapidly test efficacy in vivo. there have been indications that cotransfer of target::reporter gene fusions with constructs designed against the target sequence, followed by quantification of transient reporter gene activity might be effective. here, we report detailed testing of the approach in plants, using di ... | 2010 | 20102531 |
| toward a quarter century of pathogen-derived resistance and practical approaches to plant virus disease control. | the concept of pathogen-derived resistance (pdr) describes the use of genetic elements from a pathogen's own genome to confer resistance in an otherwise susceptible host via genetic engineering [j. theor. biol. 113 (1985) 395]. illustrated with the bacteriophage qbeta in escherichia coli, this strategy was conceived as a broadly applicable approach to engineer resistance against pathogens. for plant viruses, the concept of pdr was validated with the creation of tobacco plants expressing the coat ... | 2010 | 20109666 |
| asymmetrical over-infection as a process of plant virus emergence. | disentangling the role of epidemiological factors in plant pathogen emergences is a prerequisite to identify the most likely future invaders. an example of emergence was recently observed in france: in 10 years, "classic" (cl) strains of watermelon mosaic virus (wmv) were displaced at a regional scale by newly introduced "emerging" (em) strains. here we analyse a 3 years dataset describing the co-dynamics of cl and em strains at field scale using state-space models estimating jointly: (i) probab ... | 2010 | 20435046 |
| barriers to gene flow between emerging populations of watermelon mosaic virus in southeastern france. | since 1999, "emerging" (em) strains of watermelon mosaic virus (wmv) have been detected in cucurbit crops of southeastern france, probably as a result of recent introductions. population genetic approaches were used to study the structure of em isolates in southeastern france and to identify factors involved in their spatial distribution. a population clustering method (samova) and a maximum-difference algorithm (monmonier's algorithm) were combined to visualize and quantify barriers to gene flo ... | 2010 | 20879843 |
| recombination in natural populations of watermelon mosaic virus: new agronomic threat or damp squib? | since their introduction in south-eastern france around 1999, new, 'emerging' (em) strains of watermelon mosaic virus (wmv) coexist with the 'classic' (cl) strains present for more than 40 years. this situation constitutes a unique opportunity to estimate the frequency of recombinants appearing in the few years following introduction of new strains of a plant rna virus. molecular analyses performed on more than 1000 isolates from epidemiological surveys (2004-2008) and from experimental plots (2 ... | 2011 | 21471312 |
| search for factors involved in the rapid shift in watermelon mosaic virus (wmv) populations in south-eastern france. | watermelon mosaic virus (wmv, genus potyvirus, family potyviridae) was reported for the first time in france in 1974, and it is now the most prevalent virus in cucurbit crops. in 2000, new strains referred as 'emerging' (em) strains were detected in south-eastern france. em strains are generally more severe and phylogenetically distinct from those previously reported in this country and referred as 'classic' (cl) strains. since 2000, em strains have been progressively replacing cl strains in sev ... | 2011 | 21605606 |
| simple sequence repeat markers linked to qtl for resistance to watermelon mosaic virus in melon. | a population of recombinant inbred lines (ril) derived from a cross between the watermelon mosaic virus (wmv) resistant genotype tgr-1551 and the susceptible spanish cultivar 'bola de oro' has been evaluated for wmv resistance in spring, fall and growth chamber conditions. the quantitative trait loci (qtl) analyses detected one major qtl (wmv) on linkage group (lg) xi close to the microsatellite marker cmn04_35. this qtl controls the resistance to wmv in the three environmental conditions evalua ... | 2011 | 21811820 |
| microarray analysis shows that recessive resistance to watermelon mosaic virus in melon is associated with the induction of defense response genes. | resistance to watermelon mosaic virus (wmv) in melon (cucumis melo l.) accession tgr-1551 is characterized by a significant reduction in virus titer, and is inherited as a recessive, loss-of-susceptibility allele. we measured virus rna accumulation in tgr-1551 plants and a susceptible control ('tendral') by real-time quantitative polymerase chain reaction, and also profiled the expression of 17,443 unigenes represented on a melon microarray over a 15-day time course. the virus accumulated to hig ... | 2012 | 21970693 |
| development of transgenic watermelon resistant to cucumber mosaic virus and watermelon mosaic virus by using a single chimeric transgene construct. | watermelon, an important fruit crop worldwide, is prone to attack by several viruses that often results in destructive yield loss. to develop a transgenic watermelon resistant to multiple virus infection, a single chimeric transgene comprising a silencer dna from the partial n gene of watermelon silver mottle virus (wsmov) fused to the partial coat protein (cp) gene sequences of cucumber mosaic virus (cmv), cucumber green mottle mosaic virus (cgmmv) and watermelon mosaic virus (wmv) was construc ... | 2011 | 22203520 |
| identification and characterisation of dof transcription factors in the cucumber genome. | cucumber is vulnerable to many foliage diseases. recent studies reported cloning of candidate genes for several diseases in cucumber; however, the exact defence mechanisms remain unclear. dof genes have been shown to play significant roles in plant growth, development, and responses to biotic and abiotic stresses. dof genes coding for plant-specific transcription factors can promote large-scale expression of defence-related genes at whole genome level. the genes in the family have been identifie ... | 2016 | 26979661 |
| inheritance of resistance to potyviruses in phaseolus vulgaris l. iv. inheritance, linkage relations, and environmental effects on systemic resistance to four potyviruses. | we have examined the genetics of systemic resistance in phaseolus vulgaris to azuki bean mosaic virus (azmv) and cowpea aphid-borne mosaic virus (cabmv) and the relationship of this resistance to a phenotypically similar resistance to watermelon mosaic virus (wmv) and soybean mosaic virus (smv). in p. vulgaris cv 'great northern 1140' (gn1140), resistance to smv and wmv has been attributed to the genes smv and wmv, respectively, which have been shown to segregate as a unit. systemic resistance t ... | 1996 | 24166169 |
| recombinant soybean mosaic virus is prevalent in chinese soybean fields. | recombinant soybean mosaic virus (smv-r) is a novel strain that has recently been identified. smv-r was first isolated from chongqing, china, and exhibits different pathogenicity on soybeans and common beans compared with normal soybean mosaic virus (smv-n). smv-r arose from a recombination event between smv and bean common mosaic virus (bcmv) or a bcmv-like virus. in this study, we assessed the prevalence of smv-r in chinese soybean fields. polymerase chain reaction results showed that smv-r wa ... | 2014 | 24445813 |
| inheritance of resistance to potato y viruses in phaseolus vulgaris l : 1. two independent genes for resistance to watermelon mosaic virus-2. | resistance to watermelon mosaic virus-2 in phaseolus vulgaris l. is conferred by two distinct dominant alleles at independent loci. based on segregation data one locus is designated wmv, the other, hsw. the dominant allele wmv from cv. great northern 1140 prevents systemic spread of the virus but viral replication occurs in inoculated tissue. in contrast, hsw confers both local and systemic resistance to wmv-2 below 30c. at higher temperatures, plants that carry this allele in the absence of mod ... | 1987 | 24240215 |
| simultaneous multiplex pcr detection of seven cucurbit-infecting viruses. | two multiplex polymerase chain reaction (pcr) systems using dual priming oligonucleotide (dpo) primers were developed for the simultaneous detection of seven cucurbit-infecting viruses. one system allows for the detection of papaya ringspot virus, watermelon mosaic virus, and zucchini yellow mosaic virus, whereas the other permits the detection of cucumber green mottle mosaic virus, cucumber fruit mottle mosaic virus, kyuri green mottle mosaic virus, and zucchini green mottle mosaic virus. viral ... | 2014 | 24937806 |
| transcriptomic profiling of melon necrotic spot virus-infected melon plants revealed virus strain and plant cultivar-specific alterations. | viruses are among the most destructive and difficult to control plant pathogens. melon (cucumis melo l.) has become the model species for the agriculturally important cucurbitaceae family. approaches that take advantage of recently developed genomic tools in melon have been extremely useful for understanding viral pathogenesis and can contribute to the identification of target genes for breeding new resistant cultivars. in this work, we have used a recently described melon microarray for transcr ... | 2016 | 27267368 |
| differential life history trait associations of aphids with nonpersistent viruses in cucurbits. | the diversity of vectors and fleeting nature of virus acquisition and transmission renders nonpersistent viruses a challenge to manage. we assessed the importance of noncolonizing versus colonizing vectors with a 2-yr survey of aphids and nonpersistent viruses on commercial pumpkin farms. we quantified aphid alightment using pan traps, while testing leaf samples with multiplex rt-pcr targeting cucumber mosaic virus (cmv), zucchini yellow mosaic virus (zymv), watermelon mosaic virus (wmv), and pa ... | 2015 | 26313961 |
| antiviral activity of thuja orientalis extracts against watermelon mosaic virus (wmv) on citrullus lanatus. | watermelon mosaic potyvirus (wmv) is considered as an important virus infecting watermelon and causing adverse effects on crop productivity. to overcome this problem one of the main objectives of plant breeders is to make these strains less effective in the ability to infect plants by treatment with plant extracts. due to the advantages of plant tissue culture, in vitro, in the process of the selection of different cultivars under biotic stress, this study was conducted to achieve this aim by ev ... | 2015 | 25737655 |
| melon rna interference (rnai) lines silenced for cm-eif4e show broad virus resistance. | efficient and sustainable control of plant viruses may be achieved using genetically resistant crop varieties, although resistance genes are not always available for each pathogen; in this regard, the identification of new genes that are able to confer broad-spectrum and durable resistance is highly desirable. recently, the cloning and characterization of recessive resistance genes from different plant species has pointed towards eukaryotic translation initiation factors (eif) of the 4e family a ... | 2012 | 22309030 |
| genome sequence analysis of two south african isolates of moroccan watermelon mosaic virus infecting cucurbits. | moroccan watermelon mosaic virus (mwmv) has been prevalent in cucurbits in the republic of south africa (rsa) since it was first reported in 1987. however, full genome studies of the south african isolates have never been conducted previously. the full genome of two mwmv isolates infecting cucurbits (cucurbita pepo l.) in the province of kwazulu-natal, rsa, was compared with the genome of the tunisian isolate in this communication. the genome sequences of the rsa mwmv isolates were elucidated us ... | 2016 | 27448681 |
| zucchini shoestring virus: a distinct potyvirus in the papaya ringspot virus cluster. | zucchini shoestring virus (zssv) has been proposed to be a putative potyvirus in the papaya ringspot virus (prsv) cluster, based on the sequence similarity of its coat protein to those of related potyviruses. zssv has been associated with the outbreak of a damaging disease of baby marrow (cucurbita pepo l.) that had been observed throughout the province of kwazulu-natal, in the republic of south africa (rsa). we report the genome sequence of zssv, determined by next-generation sequencing of tota ... | 2016 | 27216927 |
| a simple, rapid and efficient way to obtain infectious clones of potyviruses. | the availability of an infectious cdna clone is a prerequisite for genetic studies on rna viruses. however, despite important improvement in molecular biology techniques during the last decades, obtaining such clones often remains tedious, time-consuming and rather unpredictable. in the case of potyviruses, cdna clones are frequently unstable due to the toxicity of some viral proteins for bacteria. the problem can be overcome by inserting introns into the viral sequence but this requires additio ... | 2012 | 22498260 |
| inheritance of resistance to watermelon mosaic virus in the cucumber line tmg-1: tissue-specific expression and relationship to zucchini yellow mosaic virus resistance. | the inbred cucumber (cucumis sativus l.) line tmg-1 is resistant to three potyviruses:zucchini yellow mosaic virus (zymv), watermelon mosaic virus (wmv), and the watermelon strain of papaya ringspot virus (prsv-w). the genetics of resistance to wmv and the relationship of wmv resistance to zymv resistance were examined. tmg-1 was crossed with wi-2757, a susceptible inbred line. f1, f2 and backcross progeny populations were screened for resistance to wmv and/or zymv. two independently assorting f ... | 1995 | 24169900 |
| characterization of a soybean mosaic virus variant causing different diseases in glycine max and nicotiana benthamiana. | we discovered a soybean mosaic virus (smv) variant (4278-1) that caused systemic infections in nicotiana benthamiana plants, resulting in stem stunting and leaf shriveling. the virus had a particle morphology and incubation period similar to those of other smv isolates but differed from them in the leaf symptoms it caused when infecting soybean and n. benthamiana. the genome of this variant consisted of a 9994-nt single-stranded rna, which was different from most of the other known smv isolates ... | 2017 | 27804020 |
| small rna profiles from virus-infected fresh market vegetables. | functional small rnas, such as short interfering rnas (sirnas) and micrornas (mirnas), exist in freshly consumed fruits and vegetables. these sirnas can be derived either from endogenous sequences or from viruses that infect them. symptomatic tomatoes, watermelons, zucchini, and onions were purchased from grocery stores and investigated by small rna sequencing. by aligning the obtained small rna sequences to sequences of known viruses, four different viruses were identified as infecting these fr ... | 2014 | 25389086 |
| potyviruses differ in their requirement for tor signalling. | potyviruses are important plant pathogens that rely on many plant cellular processes for successful infection. tor (target of rapamycin) signalling is a key eukaryotic energy-signalling pathway controlling many cellular processes such as translation and autophagy. the dependence of potyviruses on active tor signalling was examined. arabidopsis lines downregulated for tor by rnai were challenged with the potyviruses watermelon mosaic virus (wmv) and turnip mosaic virus (tumv). wmv accumulation wa ... | 2015 | 25979731 |
| first complete genome sequence of a watermelon mosaic virus isolated from watermelon in the united states. | watermelon mosaic virus was first reported in 1965 from the rio grande valley, tx. we report here the first complete genome sequence of a watermelon mosaic virus isolate from watermelon collected from the rio grande valley of texas. | 2016 | 27103724 |
| capsicum annum, a new host of watermelon mosaic virus. | the occurrence of watermelon mosaic virus (wmv) in sweet pepper (capsicum annuum l.) in kurdistan province, iran was confirmed by reverse transcriptase-polymerase chain reaction (rt-pcr) and partial characterization of coat protein. to the best of our knowledge, this is the first report of wmv infecting c. annuum, adding a new host to list of more than 170 species infected by this virus. | 2016 | 26925452 |
| cloning of the arabidopsis rwm1 gene for resistance to watermelon mosaic virus points to a new function for natural virus resistance genes. | arabidopsis thaliana represents a valuable and efficient model to understand mechanisms underlying plant susceptibility to viral diseases. here, we describe the identification and molecular cloning of a new gene responsible for recessive resistance to several isolates of watermelon mosaic virus (wmv, genus potyvirus) in the arabidopsis cvi-0 accession. rwm1 acts at an early stage of infection by impairing viral accumulation in initially infected leaf tissues. map-based cloning delimited rwm1 on ... | 2014 | 24930633 |
| comparative molecular epidemiology provides new insights into zucchini yellow mosaic virus occurrence in france. | zucchini yellow mosaic virus (zymv, genus potyvirus) causes important crop losses in cucurbits worldwide. in france, zymv epidemics are sporadic but occasionally very severe. this contrasts with watermelon mosaic virus (wmv, genus potyvirus) which causes regular and early epidemics. factors influencing zymv epidemiology are still poorly understood. in order to gain new insights on the ecology and epidemiology of this virus, a 5-year multilocation trial was conducted in which zymv spread and popu ... | 2014 | 24486486 |
| serological and molecular studies of a novel virus isolate causing yellow mosaic of patchouli [pogostemon cablin (blanco) benth]. | here we have identified and characterized a devastating virus capable of inducing yellow mosaic on the leaves of patchouli [pogostemon cablin (blanco) benth]. the diagnostic tools used were host range, transmission studies, cytopathology, electron microscopy, serology and partial coat protein (cp) gene sequencing. evidence from biological, serological and sequence data suggested that the causal virus belonged to genus potyvirus, family potyviridae. the isolate, designated as patchouli yellow mos ... | 2013 | 24386278 |
| a short motif in the n-terminal part of the coat protein is a host-specific determinant of systemic infectivity for two potyviruses. | although the biological variability of watermelon mosaic virus is limited, isolates from the three main molecular groups differ in their ability to infect systemically chenopodium quinoa. mutations were introduced in a motif of three or five amino acids located in the n-terminal part of the coat protein, and differing in isolates from group 1 (motif: lysine-glutamic acid-alanine (lys-glu-ala) or kea, systemic on c. quinoa), group 2 (lys-glu-thr or ket, not systemic on c. quinoa) and group 3 (kek ... | 2014 | 24118745 |
| outcomes of co-infection by two potyviruses: implications for the evolution of manipulative strategies. | recent studies have documented effects of plant viruses on host plants that appear to enhance transmission by insect vectors. but, almost no empirical work has explored the implications of such apparent manipulation for interactions among co-infecting pathogens. we examined single and mixed infections of two potyviruses, watermelon mosaic virus (wmv) and zucchini yellow mosaic virus (zymv), that frequently co-occur in cucurbitaceae populations and share the same aphid vectors. we found that zymv ... | 2013 | 23407835 |
| robust rnai-mediated resistance to infection of seven potyvirids in soybean expressing an intron hairpin nib rna. | viral pathogens, such as soybean mosaic virus (smv), are a major constraint in soybean production and often cause significant yield loss and quality deterioration. engineering resistance by rnai-mediated gene silencing is a powerful strategy for controlling viral diseases. in this study, a 248-bp inverted repeat of the replicase (nuclear inclusion b, nib) gene was isolated from the smv sc3 strain, driven by the leaf-specific rbcs2 promoter from phaseolus vulgaris, and introduced into soybean. th ... | 2017 | 28840434 |
| rnai-mediated smv p3 cistron silencing confers significantly enhanced resistance to multiple potyvirus strains and isolates in transgenic soybean. | robust rnai-mediated resistance to multiple potyvirus strains and isolates, but not to secovirus bpmv, was conferred by expressing a short smv p3 hairpin in soybean plants. engineering resistance to multiple potyvirus strains is of great interest because of a wide variability of the virus strains, and mixed infections of multiple viruses or strains commonly associated with field grown soybean. in this study, rnai-mediated silencing of the soybean mosaic virus (smv) p3 cistron, which is reported ... | 2017 | 28756582 |
| impact of vat resistance in melon on viral epidemics and genetic structure of virus populations. | cultivar choice is at the heart of cropping systems and resistant cultivars should be at the heart of disease management strategies whenever available. they are the easiest, most efficient and environmentally friendly way of combating viral diseases at the farm level. among the melon genetic resources, vat is a unique gene conferring resistance to both the melon aphid aphis gossypii and the viruses it carries. the 'virus side' of this pleiotropic phenotype is seldom regarded as an asset for viru ... | 2017 | 28587865 |
| new species in the papaya ringspot virus cluster: insights into the evolution of the prsv lineage. | the "papaya ringspot virus (prsv) cluster" of cucurbit-infecting potyviruses contains five acknowledged species that have similar biological, serological and molecular properties. additional data suggest there are other uncharacterized species from various locations in the world that likely belong to the prsv cluster including a new prsv-like virus reported from sudan in 2003. molecular and biological data indicated that the virus from sudan belongs to a new species, tentatively named wild melon ... | 2017 | 28669763 |