| infectivity and complete nucleotide sequence of cucumber fruit mottle mosaic virus isolate cm cdna. | three isolates of cucumber fruit mottle mosaic virus (cfmmv) were collected from melon, cucumber, and pumpkin plants in korea. a full-length cdna clone of cfmmv-cm (melon isolate) was produced and evaluated for infectivity after t7 transcription in vitro (pt7cf-cmflc). the complete cfmmv genome sequence of the infectious clone pt7cf-cmflc was determined. the genome of cfmmv-cm consisted of 6,571 nucleotides and shared high nucleotide sequence identity (98.8 %) with the israel isolate of cfmmv. b ... | 2014 | 24473709 |
| [complete genomic sequence of a watermelon isolate of cucumber green mottle mosaic virus in northern china]. | the complete genomic sequence of a watermelon isolate of cucumber green mottle mosaic virus (cgmmv-ln) in liaoning province was determined and compared with other cucurbit-infecting tobamoviruses. the genomic rna of cgmmv-ln comprised 6422 nt, and 5'- and 3'- noncoding regions consisted of 59 nt and 175 nt, respectively. the encoded four proteins were two replicase proteins of 186 kd and 129 kd, move protein of 29 kd and coat protein of 17.4 kd. the alignment results of complete nucleotide seque ... | 2009 | 19437890 |
| genome structure and production of biologically active in vitro transcripts of cucurbit-infecting zucchini green mottle mosaic virus. | abstract the complete nucleotide sequence of the zucchini green mottle mosaic virus (zgmmv), a new member of the genus tobamovirus, has been determined. the genome of zgmmv is 6,513 nucleotides long and contains four open reading frames coding for proteins of 131, 189, 28, and 17 kda from the 5' to 3' end, respectively. the 5'- and 3'-non-translated regions consist of 59 and 163 residues, respectively. the sequences of the viral proteins exhibit high identity to the proteins of the members of th ... | 2002 | 18943088 |
| simultaneous multiplex pcr detection of seven cucurbit-infecting viruses. | two multiplex polymerase chain reaction (pcr) systems using dual priming oligonucleotide (dpo) primers were developed for the simultaneous detection of seven cucurbit-infecting viruses. one system allows for the detection of papaya ringspot virus, watermelon mosaic virus, and zucchini yellow mosaic virus, whereas the other permits the detection of cucumber green mottle mosaic virus, cucumber fruit mottle mosaic virus, kyuri green mottle mosaic virus, and zucchini green mottle mosaic virus. viral ... | 2014 | 24937806 |
| biological and molecular characterization of a new cucurbit-infecting tobamovirus. | abstract an uncharacterized virus was isolated from greenhouse-grown cucumber plants. biological and serological data described in the present study indicated that the virus belonged in the genus tobamovirus. the host range of the virus included several plant species within the family cucurbitaceae. the virus designated cucumber fruit mottle mosaic virus (cfmmv) causes severe mottling or mosaic on cucumber fruits, and its fast spread within greenhouses could lead to significant economic losses i ... | 2001 | 18943945 |
| plant virus cdna chip hybridization for detection and differentiation of four cucurbit-infecting tobamoviruses. | a plant virus cdna chip was developed by using viral cdna clones and microarray technology. the cdna chip was designed for detection and differentiation of the four species of selected cucurbit-infecting tobamoviruses [target viruses: cucumber green mottle mosaic virus (cgmmv); cucumber fruit mottle mosaic virus (cfmmv); kyuri green mottle mosaic virus (kgmmv); and zucchini green mottle mosaic virus (zgmmv)]. the chip consisted of cdna clones of the four cucurbit-infecting tobamoviruses, two tar ... | 2003 | 12757916 |
| identification of the subgenomic promoter of the coat protein gene of cucumber fruit mottle mosaic virus and development of a heterologous expression vector. | heterologous gene expression using plant virus vectors enables research on host-virus interactions and the production of useful proteins, but the host range of plant viruses limits the practical applications of such vectors. here, we aimed to develop a viral vector based on cucumber fruit mottle mosaic virus (cfmmv), a member of the genus tobamovirus, whose members infect cucurbits. the subgenomic promoter (sgp) in the coat protein (cp) gene, which was used to drive heterologous expression, was ... | 2016 | 26976138 |