Publications

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responsiveness of the slug (limax maximus) to injections of fluorescein- and rhodamine-conjugated immunogens. 1978710668
natural infection of land-snails by protostrongylids on a pasture grazed by sheep in the rabat area of morocco.the output by sheep of first-stage protostrongylid larvae, and the population density and level of infection of terrestrial molluscs were assessed monthly during a 1-year study. limax maximus was the most infected mollusc. juveniles were less infected than adults, for cernuella virgata and theba pisana; the reverse was observed for otala lactea. banded individuals had a lower intensity of infection than the non-banded ones for c. virgata and t. pisana. sixty-one percent of protostrongylid larvae ...19883347990
post-ingestive food-aversion learning to amino acid deficient diets by the terrestrial slug limax maximus.an agar-based artificial diet containing carbohydrates, fats and twenty amino acids was constructed. this diet is highly palatable and nutritionally complete for limax maximus as demonstrated by significant ingestion on first encounter, consistent ingestion on subsequent days and good growth of young slugs fed this diet. removing methionine, an essential amino acid, from the complete diet produces a food which is initially as palatable as the complete diet, but after one day's intake the amount ...19863772825
certain bacteria, some of medical interest, associated with the slug limax maximus. 19704315600
immunological and biological characteristics of the vasotocin-like activity in the head ganglia of gastropod molluscs.extracts of cerebral and pleuro-pedal ganglia from two terrestrial slugs, ariolimax columbianus and limax maximus, and from the marine opisthobranch, aplysia californica, contain immunoreactivity resembling that of a vasotocin or vasopressin. radioimmunoassays using several antisera indicate that the immunoreactivity is not due to vasotocin, vasopressin, or any other known naturally occurring neurohypophyseal peptide. immunoreactivity of extracts on a relatively nonspecific vasopressin antiserum ...19846724297
localization of[3h]-2-deoxyglucose in single molluscan neurones.the glucose analogue 2-deoxyglucose (2-dg) can be used quantitatively to measure metabolic activity and is widely used qualitatively for mapping functional activity in the brain. the resolution (meaning the full width at half maximum of the grain density distribution around a line source) of the technique using [14c]-2-dg and x-ray film is limited to about 100 micrometers. attempts have been made to improve the resolution using [3h]-2-dg (ref. 6) and cellular resolution has been achieved in the ...19807432469
on the diversity of mollusc intermediate hosts of angiostrongylus costaricensis morera & cespedes, 1971 in southern brazil.veronicellid slugs are considered the most important intermediate hosts of angiostrongylus costaricensis, an intra-arterial nematode of rodents. studies undertaken in three localities in southern brazil led to identification of molluscs other than veronicellid slugs as hosts of a. costaricensis: limax maximus, limax flavus and bradybaena similaris. these data indicate a low host specificity of larval stages of a. costaricensis, as it has been reported to other congeneric species.19938107609
neurons in a variety of molluscs react to antibodies raised against the vd1/rpd2 alpha-neuropeptide of the pond snail lymnaea stagnalis.the vd1 and rpd2 neurons of lymnaea stagnalis innervate other central neurons, certain skin areas, the pneumostome area, and the auricle of the heart. recently, a set of four (delta, epsilon, alpha, beta) neuropeptides produced by these giant neurons and by certain other central neurons has been characterized. although alternative splicing of the preprohormone of these neurons yields at least 10 different alpha neuropeptides, an affinity-purified antiserum directed against a domain common to all ...19938364974
oscillations and gaseous oxides in invertebrate olfaction.olfactory systems combine an extraordinary molecular sensitivity with robust synaptic plasticity. central neuronal circuits that perform pattern recognition in olfaction typically discriminate between hundreds of molecular species and form associations between odor onsets and behavioral contingencies that can last a lifetime. two design features in the olfactory system of the terrestrial mollusk limax maximus may be common elements of olfactory systems that display the twin features of broad mol ...19968727987
determination of diphacinone residues in hawaiian invertebrates.a reversed-phase ion-pair liquid chromatographic analysis combined with a solid-phase extraction clean-up method is used to assess the quantity of diphacinone residue found in invertebrates. three invertebrate species are exposed to commercially available diphacinone-fortified bait used for rat control. the invertebrate samples are collected, frozen, and shipped to the laboratory. the samples are homogenized after cryogenic freezing. a portion of the homogenized samples are extracted with acidif ...200616599403
neutral n-glycan patterns of the gastropods limax maximus, cepaea hortensis, planorbarius corneus, arianta arbustorum and achatina fulica.the n-glycosylation potentials of limax maximus, cepaea hortensis, planorbarius corneus, arianta arbustorum and achatina fulica were analysed by investigation of the n-glycan structures of the skin and viscera glycoproteins by a combination of hplc and mass-spectrometry methods. it is one of the first steps to enlarge the knowledge on the glycosylation abilities of gastropods, which may help to establish new cell culture systems, to uncover new means for pest control for some species, and to ide ...200717516162
the occurrence of angiostrongylus vasorum in terrestrial slugs from forests and parks in the copenhagen area, denmark.a total of 298 slugs belonging to four species, arion lusitanicus, a. ater, a. ater rufus and limax maximus, were collected from six different localities within a radius of 30 km from copenhagen and examined for naturally acquired angiostrongylus vasorum infection. overall, 28 slugs (9%) were infected, but the prevalence varied among the studied localities: rude forest (26%), west amager forest (18%), jaegersborg forest and deer park (8%), frederiksberg park (4%), assistens cemetery park (0%) an ...200919460193
cat genotype tritrichomonas foetus survives passage through the alimentary tract of two common slug species.tritrichomonas foetus has recently been recognised as the cause of large bowel diarrhoea in cats (feline trichomoniasis), for which the epidemiology is largely unknown. we tested whether garden slugs common in sydney, australia can pass viable t. foetus in their faeces after oral intake. first, leopard slugs (limax maximus) were offered cat food with 10(4) of t. foetus per gram, 63% (5/8) of slugs consumed food with t. foetus and subsequently 20% (1/5) shed t. foetus in their faeces. furthermore ...201021185122
immunohistochemical demonstration of cholinergic structures in central ganglia of the slug (limax maximus, limax valentianus).immunohistochemical techniques were used to study the distribution of cholinergic neurons containing choline acetyltransferase of the common type (cchat), the synthetic enzyme of acetylcholine, in the central nervous system of the slug limax maximus and limax valentianus. because the antiserum applied here was raised against a recombinant protein encoded by exons 7 and 8 of the rat gene for chat, three methods were used in order to validate antibody specificity for the limax counterpart enzyme. ...201121315127
distribution of fmrfamide-like immunoreactivity in the nervous system of the slug limax maximus.the distribution of fmrfamide-like immunoreactive neurons in the nervous system of the slug limax maximus was studied using immunohistochemical methods. approximately one thousand fmrfamide-like immunoreactive cell bodies were found in the central nervous system. ranging between 15 micron and 200 micron in diameter, they were found in all 11 ganglia of the central nervous system. fmrfamide-like immunoreactive cell bodies were also found at peripheral locations on buccal nerve roots. fmrfamide-li ...20123416348
the molluscan neuropeptide, scpb, increases the responsiveness of the feeding motor program of limax maximus.small cardioactive peptide b (scpb) has an excitatory effect on both buccal neurons and musculature in numerous molluscan species. the present study reports the effects of scpb on the activity of specified buccal neurons and the expression of the feeding motor program of the terrestrial slug, limax maximus. superfusion of an isolated cns preparation with 10(-6)m scpb results in a 3-4-fold increase in the burst frequency of the fast salivary burster neuron (fsb), while having no effect on the act ...20123346657
gastropod-derived haemocyte extracellular traps entrap metastrongyloid larval stages of angiostrongylus vasorum, aelurostrongylus abstrusus and troglostrongylus brevior.phagocyte-derived extracellular traps (ets) were recently demonstrated mainly in vertebrate hosts as an important effector mechanism against invading parasites. in the present study we aimed to characterize gastropod-derived invertebrate extracellular phagocyte trap (inept) formation in response to larval stages of important canine and feline metastrongyloid lungworms. gastropod haemocytes were isolated from the slug species arion lusitanicus and limax maximus, and the snail achatina fulica, and ...201728143510
pedal sole immunoreactive axons in terrestrial pulmonates: limax, arion, and helix.a century ago histological techniques such as formic acid-gold chloride showed the nerve morphology of the pedal sole in limax and helix. there have been no similar descriptions since then of the central nervous system relevant to locomotory pedal waves in the foot of slugs and snails. topical application of 5-ht affects locomotory waves, but the innervation of the pedal sole with 5-ht axons is not known. three-dimensional morphology of pedal axons in terrestrial pulmonate embryos is shown herei ...201424648204
localization of the neuropeptide apgwamide in gastropod molluscs by in situ hybridization and immunocytochemistry.the amidated tetrapeptide ala-pro-gly-trp-nh2 (apgwamide) plays a key role in the control of male copulation behavior in the basommatophoran pulmonate freshwater snail lymnaea stagnalis. the morphological basis for a conserved role of apgwamide in the control of male reproduction in gastropod molluscs is presented. the prosobranch littorina littorea, the opisthobranch aplysia californica, the basommatophoran pulmonate bulinus truncatus, and the stylommatophoran pulmonates arion ater and limax ma ...19989473361
o-glycosylation of snails.the glycosylation abilities of snails deserve attention, because snail species serve as intermediate hosts in the developmental cycles of some human and cattle parasites. in analogy to many other host-pathogen relations, the glycosylation of snail proteins may likewise contribute to these host-parasite interactions. here we present an overview on the o-glycan structures of 8 different snails (land and water snails, with or without shell): arion lusitanicus, achatina fulica, biomphalaria glabrata ...201222581130
identification of putative egg-laying hormone containing neuronal systems in gastropod molluscs.of gastropod molluscs, only in the aplysiidae and the lymnaeidae have the genes encoding the respective egg-laying hormones been cloned and the neurons controlling egg laying and egg-laying behavior been identified. immunocytochemistry, using antibodies raised against alpha-cdcp (one of the neuropeptides encoded on the egg-laying hormone gene of lymnaea stagnalis), identified neurons in various species of gastropods. in the basommatophoran snail, biomphalaria glabrata, large and small neurons we ...19921505734
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