| primer extension enrichment reaction (peer): a new subtraction method for identification of genetic differences between biological specimens. | we developed a conceptually new subtraction strategy for the detection and isolation of target dna and/or rna from complex nucleic acid mixtures, called primer extension enrichment reaction (peer). peer uses adapters and class iis restriction enzymes to generate tagged oligonucleotides from dsdna fragments derived from specimens containing an unknown target ('tester'). subtraction is achieved by selectively disabling these oligonucleotides by extension reaction using ddntps and a double stranded ... | 2006 | 16790564 |
| viral and bacterial serology of six free-ranging bearded seals erignathus barbatus. | serum or heparinized plasma samples were obtained from 3 male (2 adult and 1 weaned calf) and 3 adult female free-ranging bearded seals erignathus barbatus in may of 1994, 1995, or 1996. blood samples were obtained from animals taken in subsistence hunts near st. lawrence island, alaska and screened for antibodies to a suite of bacteria and viruses potentially pathogenic for pinnipeds and/or humans. no samples had detectable antibodies to brucella spp., phocine distemper virus, influenza a virus ... | 2008 | 18828565 |
| antibodies to marine caliciviruses in the steller sea lion (eumetopias jubatus schreber). | sera from 145 steller sea lions (76 adults, three subadults, 37 pups, and 29 fetuses) were tested for neutralizing antibodies to nine marine calicivirus serotypes. antibodies were found to san miguel sea lion virus (smsv) types 1, 5, 6, 7, 8, 10 and 13, and to tillamook (bovine) calicivirus, but no antibodies were found to the walrus calicivirus. titers (microtiter neutralization assay) ranged from 1:20 to 1:320, with many positive reactions at the higher dilutions (greater than or equal to 1:80 ... | 1987 | 3820427 |
| antibodies to marine caliciviruses in the pacific walrus (odobenus rosmarus divergens illiger). | sera from 155 pacific walruses (odobenus rosmarus divergens illiger), sampled in the chukchi sea during the summer of 1983, were tested for serum neutralizing (sn) antibodies to six marine calicivirus serotypes. serotypes tested included san miguel sea lion virus (smsv) types 1, 5, 8, and 10, previously isolated from northern fur seals (callorhinus ursinus linné) in the bering sea; walrus calicivirus (wcv), previously isolated from walrus feces collected off sea ice in the chukchi sea; and tilla ... | 1986 | 3712641 |
| genomic characterization of novel marine vesiviruses from steller sea lions (eumetopias jubatus) from alaska. | marine vesiviruses were isolated in cell culture from oral and rectal swabs and vesicular fluid from alaskan steller sea lions (ssl; eumetopias jubatus). further characterization by rt-pcr, complete genomic sequencing, and phylogenetic analyses indicated that these viruses are most closely related to the marine vesiviruses, but are distinct viruses and represent two novel genotypes. the complete genome of these two ssl isolates was sequenced after cloning their viral cdna. the genomes were found ... | 2008 | 18765261 |
| new calicivirus isolated from walrus. | the nucleotide sequence and genome organization of a new member of caliciviridae was determined. cell culture inoculated with fecal matter from walrus was used to recover fragments of a new virus by suppression subtractive hybridization (ssh). the isolate was identified as a member of the vesivirus genus of caliciviridae and designated the name walrus calicivirus (wcv). sets of pcr primers spanning the entire putative genome were designed using known sequences of other vesiviruses. the assembled ... | 2004 | 15084403 |