| genome sequence determinations and analyses of novel circoviruses from goose and pigeon. | the genomes of novel circoviruses from goose and pigeon, which were isolated using degenerate primer and inverse primer pcr methods, were cloned and sequenced. comparative nucleotide (nt) sequence analyses showed that the goose circovirus (gcv) and pigeon circovirus (picv) possessed genomes which were 1821 and 2037 or 2036 nt, respectively, and which had features in common with the genomes of porcine circoviruses types 1 and 2 (pcv1, pcv2) and psittacine beak and feather disease virus (bfdv), su ... | 2001 | 11485403 |
| nucleotide sequence analysis of a novel circovirus of canaries and its relationship to other members of the genus circovirus of the family circoviridae. | the circular, single-stranded dna genome of a novel circovirus of canaries, tentatively named canary circovirus (cacv), was cloned and sequenced. sequence analysis indicated that the genome was 1952 nucleotides (nt) in size and had the potential to encode three viral proteins, including the putative capsid and replication-associated (rep) proteins. the cacv genome shared greatest sequence similarity (58.3% nt identity) with the newly characterized columbid circovirus (cocv) and was more distantl ... | 2001 | 11602793 |
| nucleotide sequences of goose circovirus isolated in taiwan. | we report the complete nucleotide (nt) sequences of eleven goose circovirus (gocv) isolated in taiwan. nine out of the eleven isolates had a genome size of 1821 nt, whereas the remaining two isolates have a size of 1820 nt. sequence comparisons of the eleven taiwanese gocv isolates and a german isolate revealed that these viruses could be divided into three distinct genetic groups. group i contains the german isolate, group ii contains three taiwanese isolates, and group iii contains eight taiwa ... | 2003 | 12745370 |
| cloning and sequencing of duck circovirus (ducv). | the genome of duck circovirus (ducv) is circular and 1996 nts in size. two major open reading frames were identified, encoding the replicase (v1) and the capsid protein (c1). a stem-loop structure comprising the nonamer 5'-tattattac, conserved in all circo-, nano- and geminiviruses, was found. unique to ducv, the region between the 3'-ends of the rep and cap gene contains four repeats of a 44-bp sequence. phylogenetic analysis shows close relation of ducv with goose circovirus and suggests class ... | 2003 | 14648300 |
| diagnosis of goose circovirus infection in hungarian geese samples using polymerase chain reaction and dot blot hybridization tests. | a polymerase chain reaction (pcr) and dot blot hybridization (dbh) test have been developed for the diagnosis of infection by a novel circovirus of geese (gocv). these tests were applied to samples of bursae of fabricius from sick and dead birds from commercial goose farms in hungary. in this second report of the occurrence of circovirus infection in diseased geese, 103 of 214 (48.1%) and 37 of 150 (24.6%) birds, and 49 of 76 (64.5%) and 18 of 76 (23.7%) flocks were positive by pcr and dbh respe ... | 2004 | 14681068 |
| avian circovirus diseases: lessons for the study of pmws. | the diseases associated with psittacine beak and feather disease virus (bfdv), pigeon circovirus (picv) and goose circovirus (gocv), which can be classified with porcine circovirus type 2 (pcv2) as members of the genus circovirus of the family circoviridae, have clinico-pathological features in common with post-weaning multisystemic wasting syndrome (pmws), with which pcv2 infection is causally associated. intracytoplasmic botryoid inclusions within macrophages and depletion of t and b lymphocyt ... | 2004 | 14741130 |
| circovirus-infected geese studied by in situ hybridization. | it has now been established that circovirus infection is common in farmed geese, but little is known about the clinicopathological significance of such infections. ten clinically diseased geese suspected of being infected by circovirus were studied by in situ hybridization using a goose circovirus dna probe. circovirus dna was demonstrated in the bursa of fabricius (bf), spleen, thymus, bone marrow, liver, kidney, lung and heart, indicating that infection can be multisystemic. in some birds, vir ... | 2005 | 16191706 |
| [cloning and analysis of the complete genome of a goose circovirus from yongkang zhejiang]. | to investigate the mechanism of avian influenza outbreak wildly in water fowls, the co-pathogens, especially that caused immuno-depression were studied. a pair of degenerated primers, which amplified a fragment of 552bp in length, was designed and synthesized based on the published goose and other circovirus sequences. the specific pcr product was amplified from the goose sample of yongkang avian influenza case of zhejiang province. the fragment was then sequenced and the result showed the exist ... | 2005 | 16496692 |
| identification of a novel circovirus in australian ravens (corvus coronoides) with feather disease. | the complete genome of a novel circovirus isolated from an australian raven (corvus coronoides) with feather lesions similar to those that occur in psittacine beak and feather disease is reported. degenerate polymerase chain reaction primers were designed to amplify and sequence novel circovirus dna from affected feathers. sequence analysis indicated that the tentatively named raven circovirus (racv) was 1898 nucleotides in size with two major open reading frames synonymous with other avian circ ... | 2006 | 16595298 |
| development of a polymerase chain reaction procedure for detection and differentiation of duck and goose circovirus. | this article reports the complete nucleotide sequences of four duck circovirus (ducv) isolates from sick ducks in taiwan and development of a polymerase chain reaction (pcr) for detection and differentiation of goose circovirus (gocv) and ducv. sequence comparison showed that taiwanese ducv isolates had 82.5%-83.8% nucleotide sequence identity to the german and north american ducv isolates. this is the first report on the presence of ducv and its associated diseases outside germany. a pcr test w ... | 2006 | 16617989 |
| serological diagnosis of goose circovirus infections. | infections with goose circovirus (gocv) are associated with growth retardation and developmental problems in farmed geese. an indirect immunofluorescence assay for detecting virus-specific serum antibody was developed for diagnostic and epidemiological purposes. in the absence of a method for growing gocv in cell culture, the assay was based on the reaction of antibodies with the gocv capsid protein produced within baby hamster kidney cells using the eukaryotic semliki forest virus expression ve ... | 2006 | 17121739 |
| genome analysis and epidemiological investigation of goose circovirus detected in eastern china. | goose circovirus (gocv), a potential immuno-suppressive virus, was detected in a sample collected from yongkang avian influenza (ai) case in zhejiang province of eastern china. further, epidemiological investigation showed that, four farms located in the two major rearing areas of yongkang and xiangshan out of eight in zhejiang province were positive for gocv. however, the geese in these gocv positive farms exhibited no apparent clinical abnormality. the complete genomes of ten isolates from yon ... | 2007 | 17619136 |
| detection of a novel circovirus in mute swans (cygnus olor) by using nested broad-spectrum pcr. | circoviruses are the causative agents of acute and chronic diseases in several animal species. clinical symptoms of circovirus infections range from depression and diarrhoea to immunosuppression and feather disorders in birds. eleven different members of the genus circovirus are known so far, which infect pigs and birds in a species-specific manner. here, a nested pcr was developed for the detection of a broad range of different circoviruses in clinical samples. using this assay, a novel circovi ... | 2008 | 18082907 |
| pathological observations of an experimental infection of geese with goose circovirus. | there is so far no report describing the pathogenicity of goose circovirus (gocv) following experimental infection. we report here an experimental inoculation of 21-day-old geese with a gocv polymerase chain reaction (pcr)-positive sample of bursa of fabricius (bf) homogenate, and the results of clinicopathological changes. forty geese were randomly assigned to two groups: the inoculated group (n=30) receiving tissue homogenate and the uninoculated group (n=10) receiving a placebo (physiological ... | 2011 | 21331948 |
| the development of a rapid sybr green i-based quantitative pcr for detection of duck circovirus. | this report describes a one-step real-time polymerase chain reaction assay based on sybr green i for detection of a broad range of duck circovirus (ducv). align with all ducv complete genome sequences and other genus circovirus download from the genbank (such as goose circovirus, pigeon circovirus), the primers targets to the replicate gene of ducv were designed. the detection assay was linear in the range of 1.31 × 102-1.31 × 107 copies/μl. the reaction efficiency of the assay using the slope ( ... | 2011 | 21978576 |
| astroviruses associated with stunting and pre-hatching mortality in duck and goose embryos. | the first detection of avian nephritis virus (anv) in goose embryos and of turkey astrovirus-1 (tastv-1) in duck embryos is described. intestinal samples from duck and goose embryos from five duck and four goose flocks in croatia were tested by polymerase chain reaction for the presence of anv, tastv-1, turkey astrovirus-2, chicken astrovirus, duck astrovirus and also for the presence of avian reovirus, derzsy's disease virus and duck enteritis virus. the kidneys from duck and goose embryos were ... | 2012 | 22845326 |
| goose parvovirus and circovirus coinfections in ornamental ducks. | clinical observations and diagnostic procedures carried out to elucidate the cause of high mortality in 2-8-wk-old ornamental ducks (mandarin, wood, falcated, and silver teal ducks) are described. at necropsy, ducklings showed general pallor of skeletal and heart muscles, subcutaneous gelatinous transudates, pericarditis, ascites, and severe edema and hyperemia of lungs. histopathologic examination revealed that the most important changes were located in the crop, bursa of fabricius, and lungs w ... | 2016 | 27309298 |
| viral infections in goose flocks in poland. | the aim of this study was to determine the infectious agents isolated from infection - suspected geese sent for the diagnostic examination to national veterinary research institute. the birds were sent from goose flocks localized in different parts of poland. totally, 1,013 birds from 122 flocks were examined. the presence of goose parvovirus (gpv), goose haemorrhagic polyomavirus (ghpv), and goose circovirus (gocv) was detected by triplex pcr. the presence of gpv dna was shown in 36 flocks. the ... | 2012 | 23214374 |
| genome sequence of a diverse goose circovirus recovered from greylag goose. | a diverse goose circovirus (gocv) genome was recovered from a wild hunted greylag goose (anser anser) in poland. the genome shares 83% pairwise identity with other gocv genomes recovered from various geese from china, germany, and taiwan. | 2015 | 26227589 |
| loop-mediated isothermal amplification for the detection of goose circovirus. | goose circovirus (gcv) presents an immunosuppressive problem in production of geese. the infection's clinical symptoms include growth retardation or feathering disorders but the infection process may remain non-symptomatic what makes the infected birds more susceptible for secondary viral, bacterial and fungal infections. diagnosis of gcv infection is made by histopathological examination, dot blot hybridization, polymerase chain reaction (pcr) and real-time pcr. however these techniques require ... | 2012 | 22695123 |
| [construction and transfection experiment of a goose circovirus infectious clone]. | a pair of primers with bamh i restriction site were designed to amplify the complete genome of goose circovirus. two copies of the genome were ligated in tandem and cloned into pgem-t easy vector to construct an infectious clone named as pgemt-2gocv. the pgemt-2gocv linearized with ecor i was transfected to negative embryos and gosling with lipfectamine. pcr detection verified the proliferation of gocv in geese. some sera of the embryo transfected group were detected to be positive at 2 and 4 we ... | 2012 | 22416347 |
| occurrence and genetic diversity of cress dna viruses in wild birds: a hungarian study. | circoviruses, cycloviruses and other circular, replication-associated protein-encoding single stranded (cress) dna viruses have been detected in a variety of animal taxa. in this study, cloacal swab samples (n = 90) were examined for cress dna viruses from 31 wild bird species living at various aquatic sites in hungary to identify possible reservoirs of viruses pathogenic to domestic poultry. a total of 30 (33.3%) specimens tested positive with pan-cress dna virus specific pcr. goose circovirus ... | 2020 | 32341382 |