| cloning of human picobirnavirus genomic segments and development of an rt-pcr detection assay. | nearly full-length genomic segments 2 and a partial-length genomic segment 1 of human picobirnavirus were cloned and sequenced. the clones were derived from viruses obtained from human immunodeficiency virus (hiv)-infected patients in atlanta, georgia (strains 3-ga-91 and 4-ga-91) and a nonhiv-infected person from china (strain 1-chn-97). the picobirnavirus genomic segments lacked sequence similarities with other viral sequences in genbank and embl. comparison of genomic segment 1 from a human a ... | 2000 | 11080479 |
| molecular diversity of partial-length genomic segment 2 of human picobirnavirus. | this study was carried out in order to evaluate the efficacy of the recently developed picobirnavirus (pbv) sets of primers and to establish the phylogenetic relationships of argentine strains with pbv strains isolated in china and the usa. | 2003 | 12931028 |
| complete nucleotide sequences of two rna segments of human picobirnavirus. | picobirnaviruses are unclassified, non-enveloped, spherical, small viruses with a genome comprising two double-stranded rna segments. only incomplete sequence data on picobirnaviruses are available so far. by cloning involving single primer amplification, full-length cdnas were prepared corresponding to rna segments 1 and 2 of a picobirnavirus (strain hy005102) isolated from a stool specimen from an infant with acute non-bacterial gastroenteritis in thailand, and the complete nucleotide sequence ... | 2005 | 15847933 |
| [picobirnavirus]. | picobirnavirus is named after the small birnavirus which contains two double-stranded rna segments as a genome. however, their properties are quite different to each other. although the virus has been detected mainly from the stools of gastroenteritis patients and several mammals and birds, the pathogenicity of the virus has not been established. characterizations of the virus are hampered due to the lack in the system for multiplication of the virus in cultured cells or experimental animals. re ... | 2005 | 16557016 |
| human picobirnaviruses identified by molecular screening of diarrhea samples. | the global threat of (re)emerging infectious viruses requires a more effective approach regarding virus surveillance and diagnostic assays, as current diagnostics are often virus species specific and not able to detect highly divergent or unknown viruses. a systematic exploration of viruses that infect humans is the key to effectively counter the potential public health threat caused by new and emerging infectious diseases. the human gut is a known reservoir of a wide variety of microorganisms, ... | 2010 | 20335418 |
| evaluation of pepper mild mottle virus, human picobirnavirus and torque teno virus as indicators of fecal contamination in river water. | a reliable indicator is needed to predict and reduce the risk of infection associated with fecal contamination of surface water. since pepper mild mottle virus (pmmov), human picobirnaviruses (hpbv) and torque teno virus (ttv) have been detected at substantial levels in human feces, we explored whether detection of nucleic acids of these viruses is a suitable indicator of fecal contamination in river water. from september 2008 to december 2009, water samples (n = 111) were collected from the ruh ... | 2010 | 21074236 |
| initiation of rna polymerization and polymerase encapsidation by a small dsrna virus. | during the replication cycle of double-stranded (ds) rna viruses, the viral rna-dependent rna polymerase (rdrp) replicates and transcribes the viral genome from within the viral capsid. how the rdrp molecules are packaged within the virion and how they function within the confines of an intact capsid are intriguing questions with answers that most likely vary across the different dsrna virus families. in this study, we have determined a 2.4 å resolution structure of an rdrp from the human picobi ... | 2016 | 27078841 |
| virome diversity analysis reveals novel enteroviruses and a human picobirnavirus in stool samples from african green monkeys with diarrhea. | it is important to identify viruses in animals because most infectious diseases in humans are caused by viruses of zoonotic origin. african green monkey is a widely used non-human primate model in biomedical investigations. in this study, total rnas were extracted from stool samples of 10 african green monkeys with diarrhea. high-throughput sequencing was used to characterize viromes. pcr and sanger sequencing were used to determine the full genome sequences. great viral diversity was observed. ... | 2020 | 32165243 |