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comparative analysis of the chromosomal and genomic organization of ty1-copia-like retrotransposons in pteridophytes, gymnosperms and angiosperms.we have investigated the physical distribution of the reverse transcriptase genes of ty1-copia-like retrotransposable elements from 12 plant species belonging to different subdivisions by hybridization in situ on chromosome preparations. ty1-copia-like elements showed different and non-random hybridization patterns. a dispersed distribution throughout most of the chromosomes with reduced hybridization at some regions or with some weak clustering at other regions was found in allium cepa, beta vu ...19979037155
promoter sequences of two homologous pectin esterase genes from chinese cabbage (brassica campestris l. ssp. pekinensis) and pollen-specific expression of the gus gene driven by a promoter in tobacco plants.the promoter regions of two genomic clones, gban215-6 and gban215-12 from chinese cabbage (brassica campestris l. ssp. pekinensis), were sequenced. the nucleotide sequences of their promoter regions were compared with that of the bp19 pollen-specific gene of brassca napus. high nucleotide sequence homologies were observed among these three genes in the region between 210 bp upstream and the putative transcription start site. a sequence motif tgtggtg, which is similar to that of the pb core motif ...19979085260
phytogrowth-inhibitory activities of sulfur-containing compounds. ii. the inhibitory activities of thiosalicylic acid and dihydro-2(3h)-thiophenone-related compounds on plant growth.thiosalicylic acid (i) showed rather strong inhibitory activity on the growth of roots of all plants treated except abelmoschus esculentus moench at the concentration of 5.0 x 10(-4) m. this compound strongly inhibited the growth of the root of echinochloa utilis ohwi et yabuno even at the low concentration of 5.0 x 10(-5) m. dihydro-2(3h)-thiophenone (vii) also exhibited inhibitory activity on the growth of roots of all plants treated except glycine max merrill. both compounds inhibited the ger ...19938220332
characterization of a cdna encoding cysteine proteinase inhibitor from chinese cabbage (brassica campestris l. ssp. pekinensis) flower buds.a cdna encoding a new phytocystatin isotype named bcpi-1 was isolated from a cdna library of chinese cabbage flower buds. the bcpi-1 clone encodes 199 amino acids resulting in a protein much larger than other known phytocystatins. bcpi-1 has an unusually long c-terminus. a bcpi-1 fusion protein expressed in escherichia coli strongly inhibits the enzymatic activity of papain, a cysteine proteinase. genomic southern blot analysis revealed that the bcpi gene is a member of a small multi-gene family ...19968616262
defense-related gene induction in brassica campestris in response to defined mutants of xanthomonas campestris with altered pathogenicity.we have studied the induction of beta-1,3-glucanase (bgl) in turnip following inoculation with pathovars of xanthomonas campestris and derived mutants. bgl transcript accumulated more rapidly in leaves in the incompatible interactions with x. c. pv. armoraciae and x. c. pv. raphani than in the compatible interaction with x. c. pv. campestris. no accumulation was seen in response to wounding or inoculation with water, salicylic acid, or escherichia coli. deletion of the hrp cluster from the x. ca ...19947949324
lipopolysaccharide from xanthomonas campestris induces defense-related gene expression in brassica campestris.purified lipopolysaccharide (lps) from xanthomonas campestris pv. campestris induced accumulation of transcript for beta-1,3-glucanase in turnip at concentrations of 1 micrograms/ml. the lipid a-inner core structure was required for activity but the o-antigen had no role. we suggest that release of lps in planta triggers expression of at least some defense-related genes.19957579622
isolation and characterization of soybean waste-degrading microorganisms and analysis of fertilizer effects of the degraded products.two microorganisms which could degrade soybean lees efficiently were isolated and identified as bacillus circulans and b. stearothermophilus. these two strains secreted thermostable proteases into the medium and could digest soybean lees rapidly and completely at 50 degrees c. initially, the soybean lees were degraded to proteins in approximately 20 h by these two strains, after which time the concentrations of peptides in the medium gradually increased. the degraded products from soybean lees c ...19948117080
haploid and diploid expression of a brassica campestris anther-specific gene promoter in arabidopsis and tobacco.the anther-specific cdna clone bcp1 from brassica campestris is expressed in both the haploid pollen and diploid tapetum, as shown by in situ hybridization. we have isolated bgp1, a genomic clone homologous to bcp1. the coding region and extensive 5' flanking sequences of bgp1 have been sequenced, and the coding region shows 88% identity with bcp1. rna gel blot analysis confirmed the expression of bgp1-specific transcripts in b. campestris pollen. a 767 bp 5' dna fragment was fused to the report ...19938510662
biological activity of purpurogallin.purpurogallin showed antibacterial activity toward gram-positive bacteria. strong activity against methicillin-resistant staphylococcus aureus [minimal inhibitory concentration (mic) against methicillin of 1600 micrograms/ml] was found, with mic of 11.0 micrograms/ml. purpurogallin inhibited the growth of all tested plants and decreased the chlorophyll content in the cotyledons of brassica campestris subsp. rapa. it showed potent inhibitory activity against prolyl endopeptidase (the 50% inhibito ...19979178568
the presence of a sar1 gene family in brassica campestris that suppresses a yeast vesicular transport mutation sec12-1.two new members (bsar1a and bsar1b) of the sar1 gene family have been identified from a flower bud cdna library of brassica campestris and their functional characteristics were analyzed. the two clones differ from each other at 14 positions of the 193 amino acid residues deduced from their coding region. the amino acid sequences of bsar1a and bsar1b are most closely related to the sar1 family, genes that function early in the process of vesicle budding from the endoplasmic reticulum (er). the se ...19979154984
rhizobium leguminosarum as a plant growth-promoting rhizobacterium: direct growth promotion of canola and lettuce.early seedling root growth of the nonlegumes canola (brassica campestris cv. tobin, brassica napus cv. westar) and lettuce (lactuca sativa cv. grand rapids) was significantly promoted by inoculation of seeds with certain strains of rhizobium leguminosarum, including nitrogen- and nonnitrogen-fixing derivatives under gnotobiotic conditions. the growth-promotive effect appears to be direct, with possible involvement of the plant growth regulators indole-3-acetic acid and cytokinin. auxotrophic rhi ...19968868235
a metalloprotease from xanthomonas campestris that specifically degrades proline/hydroxyproline-rich glycoproteins of the plant extracellular matrix.culture supernatants of xanthomonas campestris pv. campestris contain an enzymic activity capable of degrading gp120, a proline-rich glycoprotein associated with the extracellular matrix of the vascular bundles in petioles of turnip (brassica campestris). this activity did not reside in any of the three previously characterized proteases of x. campestris pv. campestris that were identified by their action against the model substrate beta-casein. the novel enzyme was purified by ion-exchange and ...19989805395
purification and characterization of protein kinase c from a higher plant, brassica campestris l.protein kinase c (pkc) was partially purified from brassica campestris l., by successive chromatographies on deae-cellulose membrane, hydroxyapatite and phenyl-5pw columns. the purified preparation showed typical characteristics of the conventional type of mammalian pkc that responds to ca2+, phosphatidylserine, and diacylglycerol or the tumor-promoting phorbol ester, phorbol 12-myristate 13-acetate. the plant pkc activity was apparently associated with a 75-kda polypeptide that was recognized b ...19948074672
induction of extracellular matrix glycoproteins in brassica petioles by wounding and in response to xanthomonas campestris.a panel of monoclonal antibodies that recognize plant extracellular matrix glycoproteins previously implicated in plant-microbe interactions was used to study the effects of pathogen inoculation and wounding on glycoproteins in petioles of brassica campestris. the panel of monoclonals comprised two sets: jim11, jim12, and jim20 recognize epitopes carried on hydroxyproline-rich glycoproteins (hrgps) (m. smallwood, a. beven, n. donovan, s. j. neitl, j. peart, k. roberts, and j. p. knox, plant j. 5 ...19979304856
potential of a novel antibiotic, 2-methylheptyl isonicotinate, as a biocontrol agent against fusarial wilt of crucifers.screening for newer bioactive compounds from microbial metabolites resulted in the isolation of a novel antibiotic from the culture filtrate, streptomyces sp 201, of a soil. the bioactive compound, with antifungal and antibacterial activity, was identified as 2-methylheptyl isonicotinate. the antifungal activity of live culture, culture broth and the isolated bioactive compound showed marked inhibition against dominant soil-borne phytopathogens such as fusarium oxysporum schlect, f moniliforme s ...200211975177
comparative mapping of the brassica s locus region and its homeolog in arabidopsis. implications for the evolution of mating systems in the brassicaceae.the crucifer family includes self-incompatible genera, such as brassica, and self-fertile genera, such as arabidopsis. to gain insight into mechanisms underlying the evolution of mating systems in this family, we used a selective comparative mapping approach between brassica campestris plants homozygous for the s8 haplotype and arabidopsis. starting with markers flanking the self-incompatibility genes in brassica, we identified the homeologous region in arabidopsis as a previously uncharacterize ...19989596638
biological effects of rare earth protein complexes: influence of lanthanide ions eu3+, tb3+ on secondary structure of calmodulins.the secondary structure of four kinds of calmodulins (cams; i.e., brassica campestris pollen cam, bovine brain cam, earthworm calcium binding protein, and earthworm new calcium binding protein) in thin films are determined by the ftir resolution enhanced technique and curve fitting. the variation in the secondary structure of cam upon its binding with ca2+, eu3+, and tb3+, the assay of phosphodiesterase enzyme, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis are also investigated. ...199910604289
the tomato mosaic tobamovirus movement protein interacts with a putative transcriptional coactivator kelp.viral movement through plasmodesmata in host plants likely depends on the interaction between virus-encoded movement protein (mp) and host proteins. in order to search for mp-interacting protein (mip), we carried out far-western screening of a brassica campestris cdna library using a recombinant mp of tomato mosaic tobamovirus (tomv) as a probe. one of the positive clones, designated mip102, was found to be a putative orthologue for a transcriptional coactivator kelp of arabidopsis thaliana. in ...200111561731
growth rate, lipid composition, metabolism and myocardial lesions of rats fed rapeseed oils (brassica campestris var. arlo, echo and span, and b. napus var. oro). 19734752972
molecular cloning and characterization of the gene encoding glutathione reductase in brassica campestris.we have isolated the brassica campestris cdna encoding glutathione reductase of 502 amino acid residues with molecular mass of 54.5 kda. the deduced amino acid sequences were 92.2%, and 79.5% identical to those of arabidopsis thaliana, and pea, respectively. as expected, it exhibited a high degree of conservation within the region responsible for the redox reaction and for the binding of gssg or nadph. the gene was highly inducible by ozone fumigation or by paraquat treatment.19989512665
preliminary observations on inhalation and intradermal challenges of horses with oil seed rape.horses with asymptomatic or symptomatic chronic obstructive pulmonary disease (copd) and control horses were challenged with oil seed rape either by exposure to a field of flowering brassica campestris for six days, or by an inhalation challenge with freshly collected pollen from brassica napus, or by inhalation challenge with a commercial extract of b napus pollen. clinical and bronchoscopic examinations showed that the challenges did not induce detectable pulmonary disease in the control or as ...19921413430
immunopurification and immunocharacterization of the glucosinolate biosynthetic enzyme thiohydroximate s-glucosyltransferase.preparing homogeneous udp-glucose:thiohydroximate s-glucosyltransferase (s-gt), the penultimate biosynthetic enzyme of glucosinolates, by standard chromatographic methods has yielded too little protein for adequate purity evaluation, identity verification, and structural analysis. the low yields were apparently due to low abundance in source tissues, aggravated by enzyme instability. here we describe an immunological method for purification of workable quantities from florets of brassica olerace ...199412232212
characterization of 2s seed storage protein of brassica campestris and its antigenic homology with seed proteins of other cruciferae.the low molecular weight seed storage protein of brassica campestris has been isolated and its amino acid composition determined. antibody raised against this low molecular weight protein has been used to compare the antigenic similarity between the low molecular weight storage proteins of different cruciferae seeds by immunoprecipitation and western blotting. these studies revealed the existence of antigenically homologous proteins of identical molecular weights in seeds of other cruciferae but ...19911805785
mycotoxins produced by fusarium oxysporum in the seeds of brassica campestris during storage.three recognized mycotoxins, viz. diaxetoxyscirpenol, t-2 toxin and zearalenone, along with the fatty esters of these toxins were isolated from the flask culture extractives of fusarium oxysporum schlecht (imi 273220) as also from seeds of brassica campestris var. sarson (mustard) infected with this fungus in storage. evaluation of biological activity and toxicity of the extractives and the effects of prolonged ingestion of the mouldy seeds by animals suggest that the infected seeds may present ...19872952882
flowering plant sperm contains a cytosolic soluble protein factor which can trigger calcium oscillations in mouse eggs.there is evidence showing that the sperm-induced ca(2+) oscillations in mammalian eggs at fertilization are triggered by a sperm-derived protein factor. it was established recently that the activity of the putative sperm protein in causing ca(2+) oscillations in mammalian eggs is not species-specific in vertebrates (1, 16). here we report that cytosolic soluble extracts derived from flowering plant sperms in brassica campestris can also induce fertilization-like ca(2+) oscillations when microinj ...200111549252
[preliminary studies on tissue culture and agrobacterium-medicated transformation of brassica campestris ssp. chinensis].the hypocotyls and cotyledons of the asepetic seedling of brassica campestris ssp. chinensis l cv. pudongaijiecai) were used as explants for tissue culture. adventitious buds were differentiated on modified ms medium supplemented with tdz 1-2 mg/l, naa 0.2-1 mg/l and agno3 7.5 mg/l. the percentage of explants which formed buds of cotyledons was about 56%, and that of hypocotyls was about 37%. when the regenerated explants were transferred onto ms medium with 2 i.p. 5 mg/l and naa 0.1 mg/l for tw ...199912548800
brassica campestris var. span: ii. cardiopathogenicity of fractions isolated from span rapeseed oil when fed to male rats.rapeseed oils low in erucic acid caused myocardial lesions when fed to weanling male rats for 16 weeks. the cardiopathogenic properties appear to be associated with the triglycerides of the oil, and not to nontriglyceride components present in fully refined rapeseed oil. cardiac lipid analysis confirmed that erucic acid accumulation was proportional to the concentration of this acid in the diet.19751177663
nutritional effects of partially hydrogenated low erucic rapeseed oils.the incidence of cardiac lesions in male rats fed rapeseed oil (brassica campestris, cultivar "span") was lower with partially hydrogenated oil (iodine value 78) than withe the liquid oil which had been treated in various ways. another rapeseed oil (brassica napus, cultivar "tower") was similarly improved when hydrogenated to iodine value 76.6, but not at iodine value 97.1, as demonstrated in both sprague-dawley and wistar rats. the improved nutritional quality of hydrogenated oil appeared not t ...1977916818
single amino acid change in the helicase domain of the putative rna replicase of turnip crinkle virus alters symptom intensification by virulent satellites.the virulent satellite [satellite c (sat c)] of turnip crinkle virus (tcv) is a small pathogenic rna that intensifies symptoms in tcv-infected turnip plants (brassica campestris). the virulence of sat c is determined by properties of the satellite itself and is influenced by the helper virus. symptoms produced in infections with sat c differ in severity depending on the helper virus. the tcv-ji helper virus produces more severe symptoms than the tcv-b helper virus when inoculated with sat c. to ...19921370351
tissue printing for myrosinase activity in roots of turnip and japanese radish and horseradish: a technique for localizing myrosinases.a method for analyzing the tissue distribution of myrosinase activity in brassicaceous plants was developed. this technique is based on 'tissue printing' to visualize enzyme activity. the freshly-cut surface (transverse direction) of the root of three species, japanese radish (raphanus sativus), turnip (brassica campestris) and japanese horseradish (wasabi, wasabia japonica), was pressed onto a polyvinylidene difluoride (pvdf) filter to immobilize the proteins onto the membrane. the sites of myr ...200111166428
cdna cloning and characterization of enolase from chinese cabbage, brassica campestris ssp. pekinensis.an enolase-encoding cdna clone from chinese cabbage, brassica campestris ssp. pekinensis, was isolated. this gene (accession number: ay307448) had a total length of 1580bp with an open reading frame of 1335bp, and encoded a predicted polypeptide of 444 amino acids with a molecular weight of 47.38 kda. the deduced amino acid (aa) sequence shared identity with a number of enolases ranging from bacillus subtilis to human beings and had much higher identity with other plant enolases than with enolas ...200415354355
laboratory evaluations of a wild crucifer barbarea vulgaris as a management tool for the diamondback moth plutella xylostella (lepidoptera: plutellidae).the term 'dead-end trap cropping' has recently been proposed to identify a plant that is highly attractive for oviposition by an insect pest, but on which offspring of the pest cannot survive. the potential of the wild crucifer barbarea vulgaris r. br. to allure and serve as a dead-end trap crop for the diamondback moth plutella xylostella (l.), an important pest of cruciferous crops worldwide, was examined in laboratory experiments. when p. xylostella adults were provided with a dual-choice of ...200415541190
overexpression of a eukaryotic glutathione reductase gene from brassica campestris improved resistance to oxidative stress in escherichia coli.glutathione reductase (gr) plays an essential role in a cell's defense against reactive oxygen metabolites by sustaining the reduced status of an important antioxidant glutathione. we constructed a recombinant plasmid based on the expression vector pet-18a that overexpresses a eukaryotic gr from brassica campestris (bcgr) in escherichia coli. for comparative analyses, e. coli gr (ecgr) was also subcloned in the same manner. the transformed e. coli with the recombinant constructs accumulated a hi ...200515596144
a novel arabidopsis-colletotrichum pathosystem for the molecular dissection of plant-fungal interactions.the ability of a colletotrichum sp., originally isolated from brassica campestris, to infect arabidopsis thaliana was examined. sequence analysis of the internal transcribed spacer (its)1, 5.8s rna gene and its2 regions of ribosomal (r)dna showed the pathogen to be colletotrichum destructivum. the host range was broad, including many cruciferous plants and some legumes. at 25 degrees c, all a. thaliana accessions tested were susceptible to the brassica isolates of c. destructivum; however, at 15 ...200415000394
effect of alternaria pathotoxin(s) on expression of p53-like apoptotic protein in calli and leaves of brassica campestris.possible involvement of apoptosis was investigated in pathotoxin-treated and nutritionally-depleted in vitro cultured calli by comparing levels of p53-like protein. antibodies raised against human p53 were used to detect and quantify p53 in b. campestris. expression of p53-like protein increased from proliferating to static growth stage and reached to constant level at decaying stage. both elisa and dot immuno-binding assay showed that p53-like protein was over expressed in toxin treated and nut ...200212561976
effects of cold exposure on feed protein degradation, microbial protein synthesis and transfer of plasma urea to the rumen of sheep.1. three diets of barley-canola-seed (brassica campestris), lucerne (medicago sativa) or chopped brome-grass (bromus inermis) were given at intervals of 3 h to closely-shorn suffolk wethers held at a temperature of 1-5 degree (cold) or 22-24 degree (warm). apparent digestibility of organic matter (om) and nitrogen was reduced by 0.08-0.05 and 0.04 units respectively for lucerne and brome-grass diets given to cold-exposed sheep, but no treatment effects on digestibility were observed for the barl ...19827082623
use of waste chinese cabbage as a substrate for yeast biomass production.the possibility of using waste chinese cabbage (brassica campestris) as a substrate for microbial biomass production was investigated. the juice from waste chinese cabbage contains relatively high amounts of reducing sugars suitable for yeast culture. the cell mass and protein content of four species of yeast, candida utilis, pichia stipitis, kluyveromyces marxianus, and saccharomyces cerevisiae, were determined when cultured in juice extracted from cabbage waste. compared to ym broth containing ...200212094802
the size, shape, and structure of chinese rape (brassica campestris l.-youcai) mosaic virus, ymv15, and some chemical characteristics of its protein subunits. 196414208093
point mutations in the turnip crinkle virus capsid protein affect the symptoms expressed by nicotiana benthamiana.in an effort to determine the biological function(s) of the capsid protein protruding domains unique to the plant carmo- and tombusviruses, we constructed turnip crinkle virus (tcv) mutants in which tandem, in-frame translation terminators replaced the first two codons of the five-amino acid hinge between the shell and the protruding domains of the tcv capsid protein. one of the mutants replicated in inoculated leaves and protoplasts without detectable accumulation of capsid protein. the mutant ...19912053278
characterization of a stamen-specific cdna encoding a novel plant defensin in chinese cabbage.we isolated a stamen-specific cdna, bsd1 (brassica stamen specific plant defensin 1) that encodes a novel plant defensin peptide in chinese cabbage (brassica campestris l. ssp. pekinensis). plant defensins are antimicrobial peptides containing eight highly conserved cysteine residues linked by disulfide bridges. in bsd1, the eight cysteine residues and a glutamate residue at position 29 are conserved whereas other amino acid residues of the plant defensins consensus sequence are substituted. bsd ...200212139009
microbial succession associated with organic matter decomposition during thermophilic composting of organic waste.using dog food as a model of the organic waste, thermophilic composting was carried out for 14 days at a fixed temperature of 60 degrees c. the relationship between organic matter decomposition measured by co2 evolution during the bio-stabilization process and microbial succession expressed as the changes over time in the restriction fragment length polymorphism (rflp) patterns of 16s rdna sequences, of micro-organisms associated with the composting material was also examined. the co2 evolution ...200515751395
effects of the fermentation product of herbs by lactic acid bacteria against phytopathogenic filamentous fungi and on the growth of host plants.the fermentation product of herbs by lactic acid bacteria (fhl) was assayed for antifungal activities against rosellinia necatrix, helicobasidium mompa, fusarium oxysporum, pythium graminicola and pyricularia oryzae. fhl completely inhibited the growth of r. necatrix, h. mompa, p. graminicola and p. oryzae, and reduced the growth of f. oxysporum by 35%. when the seeds of medicago sativa l. (alfalfa), asparagus officinalis l. (asparagus), brassica campestris l. (komatsuna), oryza sativa l. (rice) ...200416233688
productive performance and liver lesions in two strains of laying hens receiving two rapeseed meals.a comparative study was conducted to determine the effect of two varieties of rapeseed meal at 10 and 20% levels in the diet on the productive performance, organ weights and liver lesions of two commercial strains of laying hens. the double-low (brassica napus 1788) rapeseed meal appeared to be superior to span meal (brassica campestris) at the 10% level in maintaining egg production, feed efficiency, thyroid weight and body weight gain. however at the 20% level the productive performance was de ...1977611494
effect of dietary cysteine supplements on canola meal toxicity and altered hepatic glutathione metabolism in the rat.experiments were conducted to determine the effects of feeding canola meal (brassica campestris and brassica napus) on the rat hepatic glutathione detoxification system and whether dietary cysteine supplements might modify such effects. rats were fed test diets for 14 d. body weight change, feed consumption, hepatic glutathione concentration, and hepatic glutathione-s-transferase (gsh-s-t) activities were determined. weight gain was decreased when canola meal was fed, whereas hepatic glutathione ...19921506312
rhizosphere competent pseudomonas aeruginosa grc1 produces characteristic siderophore and enhances growth of indian mustard (brassica campestris).pseudomonas aeruginosa grc1, an isolate of potato rhizosphere, was known to have several plant growth-promoting activities, including production of phytohormone and antibiotic substance. the isolate was found to have prolific production ability of hydroxamate siderophore in iron-deficient conditions. the siderophore of grc1 was purified and characterized. the purified siderophore appeared to be of pyoverdin type with typical amino acid composition. in field trials, p. aeruginosa grc1 enhanced th ...200516211433
tn5-mediated cloning of a genetic region from pseudomonas putida involved in the stimulation of plant root elongation.transposon (tn5) mutagenesis was applied to pseudomonas putida gr12-2r3, which promotes root elongation (a phenotype designated pre) of brassica campestris under gnotobiotic conditions. of 3,000 tn5 transconjugants, only one mutant that lost pre activity but remained prototrophic and capable of plant root colonization was detected. this mutant was complemented by plasmid pre53, which contained a 15.0-kilobase dna insert isolated from a parental strain. the complemented mutant regained full pre a ...198816347807
induction of hydrolytic enzymes in brassica campestris in response to pathovars of xanthomonas campestris.inoculation of mature leaves of turnip (brassica campestris) with the incompatible xanthomonas campestris pv vitians resulted in the induction of beta-1,3-glucanase and chitinase/lysozyme (chl) activity. no increase in the basal activity of beta-1,3-glucanase was observed after inoculation of leaves with heat- or rifampicin-killed x. c. vitians, escherichia coli, or sterile water. inoculation with the compatible x. campestris pv campestris resulted in a slower induction of glucanase than that se ...199016667441
effect of inclusion of tree leaves in feed on nutrient utilization and rumen fermentation in sheep.the effect of inclusion of tree leaves in mustard (brassica campestris) straw (ms) based complete feed blocks (cfb) on nutrient utilization and rumen fermentation was studied in adult male sheep. four types of cfb diets (roughage:concentrate:molasses, 70:25:5) were prepared. the compaction process increased bulk density (g/cm(3)) of msb, msnl, mssl and msal by 2.9, 1.79, 2.40 and 2.26 times, respectively. the dry matter intake (g/day) was higher (p<0.05) in mssl and msal than in msb. digestibili ...200716563748
relationship between erucic acid and myocardial changes in male rats.the back and belly fat of pigs fed a diet containing 20% by wt rapeseed oil (22% erucic acid) for 16 weeks was rendered into oil. this rendered pig fat, which contained 5.6% erucic acid, was fed to male rats in three separate experiments at 20% by wt of the diet for 16 weeks. in experiment i rendered pig fat was compared only to brassica campestris var. span rapeseed oil containing 4.8% erucic acid. in experiments ii and iii, rendered pig fat was compared to commercial lard containing 0.2% docos ...19761250074
lipid changes in tissues of young boars fed rapeseed oil or corn oil.fully refined rapeseed oil, brassica campestris var. arlo plus echo, and corn oil were fed at 20% by weight of a basal diet to young yorkshire boars. a serial slaughter technique was employed whereby 3 boars from each dietary treatment were killed at 0,1,2,3,4 and 8 weeks of the experiment. the fatty acid profiles of heart, liver, adrenals and testes lipids were investigated. the typical fatty acids of rapeseed oil, erucic and eicosenoic acids, were incorporated at different levels into the tiss ...19751232599
oleosin isoforms of high and low molecular weights are present in the oil bodies of diverse seed species.oleosins are unique and major proteins localized on the surface of oil bodies in diverse seed species. we purified five different oleosins (maize [zea mays l.] kd 16 and kd 18, soybean [glycine max l.] kd 18 and kd 24, and rapeseed [brassica campestris l.] kd 20), and raised chicken antibodies against them. these antibodies were used to test for immunological cross-reactivity among oleosins from diverse seed species. within the same seed species, antibodies raised against one oleosin isoform did ...199016667830
impact of sewage irrigation on speciation of nickel in soils and its accumulation in crops of industrial towns of punjab.analysis of soil samples collected from sewage and tube well irrigated soils of ludhiana, amritsar jalandhar and mandi gobindgarh, revealed that diethylene triamine pentaacetic acid extractable nickel ( dtpa-ni) was found to be higher in sewage fed soils. sewage irrigation increased soil dtpa-ni content by 3.04 times over the tube well irrigated soils. the content of dtpa-ni showed decreasing trend with depth. hydrogen concentration (ph) was negatively and significantly correlated with dtpa-ni n ...200819295085
induction of male sterile cabbage using a tapetum-specific promoter from brassica campestris l. ssp. pekinensis.the anther (tapetum)-specific gene bca9 was isolated from chinese cabbage, brassica campestris l. ssp. pekinensis cv. jangwon, using the arabidopsis tapetum-specific a9 gene as a probe. the dna and amino acid sequences of the coding region of the bca9 gene showed high homology with a9 genes from arabidopsis and b. napus. however, the dna sequences of the 5' noncoding (promoter) region were different, except for the sequence from -281 to -89. to test the specific activity of this promoter, a plan ...200312920565
molecular and functional characterization of a cyclophilin with antifungal activity from chinese cabbage.an antifungal protein that inhibits the growth of filamentous fungal pathogens was isolated from chinese cabbage (brassica campestris l. ssp. pekinensis) by affinity chromatography on affi-gel blue gel and ion exchange chromatography on cm-sepharose. the n-terminal amino acid sequence of the protein was highly homologous to that of plant cyclophilins and consequently the protein was denoted as c-cyp. to understand the antifungal activity of c-cyp, we isolated a cdna encoding its gene from a chin ...200717194440
effectiveness and stability of heterologous proteins expressed in plants by turnip mosaic virus vector at five different insertion sites.the n-terminal (nt) regions of particular protein-coding sequences are generally used for in-frame insertion of heterologous open reading frames (orfs) in potyviral vectors for protein expression in plants. an infectious cdna clone of turnip mosaic virus (tumv) isolate yc5 was engineered at the generally used nt regions of hc-pro and cp, and other possibly permissive sites to investigate their effectiveness to express the gfp (jellyfish green fluorescent protein) and der p 5 (allergen from the d ...200717689817
[preliminary findings on the endocrine changes in rabbits fed cabbage (brassica oleracea) & rape (brassica campestris oleifera)]. 195713490561
regulation of ethylene levels in canola (brassica campestris) by 1-aminocyclopropane-1-carboxylate deaminase-containing methylobacterium fujisawaense.we report the presence of acc deaminase in methylobacterium fujisawaense and its lowering of ethylene levels and promotion of root elongation in canola seedlings under gnotobiotic conditions. to test a part of the previous model proposed for acc deaminase producing bacteria with methylobacterium, acc levels and various enzyme activities were monitored in canola. lower amounts of acc were present in the tissues of seeds treated with m. fujisawaense strains than in control seeds treated with mgso( ...200616416316
[bcmf4 gene, encoding a leucine-rich repeat protein, plays a role in male fertility in chinese cabbage-pak-choi].the bcmf4 (brassica campestris male fertility 4) gene was previously isolated from the fertile b line of chinese cabbage-pak-choi (brassica campestris ssp. chinensis var. communis, syn. b. rapa ssp. chinensis var. communis). in the present paper, based on the cdna sequence of bcmf4, primers were designed and used to amplify two fragments from the cdna of flower buds of chinese cabbage-pak-choi. two produced fragments were introduced separately into binary vector pbi121 in antisense and sense ori ...200617098713
purification and characterization of an antifungal protein, c-fkbp, from chinese cabbage.an antifungal protein was isolated from chinese cabbage (brassica campestris l. ssp. pekinensis) by buffer-soluble extraction and two chromatographic procedures. the results of matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) mass spectrometry revealed that the isolated chinese cabbage protein was identical to human fk506-binding protein (fkbp). a cdna encoding fkbp was isolated from a chinese cabbage leaf cdna library and named c-fkbp. the open reading frame of the gene en ...200717550265
a steroid fraction of chloroform extract from bee pollen of brassica campestris induces apoptosis in human prostate cancer pc-3 cells.bee pollen of brassica campestris l. is widely used in china as a natural food supplement and an herbal medicine in strengthening the body's resistance against diseases including cancer. the present study was carried out to investigate the effect of a steroid fraction of chloroform extract from bee pollen of brassica campestris l. on human cancer cell viability. our studies show that among nine cancer cell lines of different origin (pc-3, lncap, mcf-7, hela, bel-7402, bcg-823, kb, a549 and ho891 ...200717639562
pedobacter suwonensis sp. nov., isolated from the rhizosphere of chinese cabbage (brassica campestris).a mesophilic bacterium, strain 15-52(t), was isolated from the rhizosphere of chinese cabbage (brassica campestris). on the basis of phenotypic and genotypic characteristics, the bacterium was identified as representing a novel species belonging to the genus pedobacter. the strain is non-flagellated, non-spore-forming and grows at temperatures in the range 1-37 degrees c. physiological tests of the strain showed the presence of oxidase, catalase, protease (gelatin and casein hydrolysis), beta-gl ...200717329772
coprinellus curtus (hitoyo-take) prevents diseases of vegetables caused by pathogenic fungi.a strain of coprinellus curtus (designated gm-21), a basidiomycete that suppressed bottom-rot disease of chinese cabbage, 'pak-choi' (brassica campestris), caused by the pathogen rhizoctonia solani pak-choi 2 was isolated. the mechanism of plant disease suppression was discovered to be hyphal interference, a combative fungal interaction between strain gm-21 and the pathogen. the antifungal spectrum of strain gm-21 was shown to include r. solani and fusarium sp., i.e. strain gm-21 showed disease- ...200717850327
lipid transfer proteins from brassica campestris and mung bean surpass mung bean chitinase in exploitability.antifungal peptides with a molecular mass of 9 kda and an n-terminal sequence demonstrating remarkable similarity to those of nonspecific lipid transfer proteins (nsltps) were isolated from seeds of the vegetable brassica campestris and the mung bean. the purified peptides exerted an inhibitory action on mycelial growth in various fungal species. the antifungal activity of brassica and mung bean nsltps were thermostable, ph-stable, and stable after treatment with pepsin and trypsin. in contrast, ...200717726719
enhanced tolerance to sulfur dioxide and salt stress of transgenic chinese cabbage plants expressing both superoxide dismutase and catalase in chloroplasts.to explore the possibility of overcoming the highly phytotoxic effect of so(2) and salt stress, we introduced the maize cu/znsod and/or cat genes into chloroplasts of chinese cabbage (brassica campestris l. ssp. pekinensis cv. tropical pride) (referred to as sod, cat and sod+cat plants). sod+cat plants showed enhanced tolerance to 400 ppb so(2), and visible damage was one-sixth that of wild-type (ck) plants. in addition, when sod+cat plants were exposed to a high salt treatment of 200 mm nacl fo ...200717851086
first isolation of an antifungal lipid transfer peptide from seeds of a brassica species.an antifungal peptide with a molecular mass of 9412 and an n-terminal sequence exhibiting notable homology to those of lipid transfer proteins was isolated from seeds of the vegetable brassica campestris. the purification protocol entailed ion exchange chromatography on q-sepharose, affinity chromatography on affi-gel blue gel, ion exchange chromatography by fast protein liquid chromatography (fplc) on mono s, and gel filtration by fplc on a superdex peptide column. the antifungal peptide was ad ...200717692430
the ecology of bacillus thuringiensis on the phylloplane: colonization from soil, plasmid transfer, and interaction with larvae of pieris brassicae.seedlings of clover (triflorium hybridum) were colonized by bacillus thuringiensis when spores and seeds were co-inoculated into soil. both a strain isolated in the vegetative form from the phylloplane of clover, 2810-s-4, and a laboratory strain, hd-1, were able to colonize clover to a density of about 1000 cfu/g leaf when seeds were sown in sterile soil and to a density of about 300 cfu/g leaf in nonsterile soil. a strain lacking the characteristic insecticidal crystal proteins produced a simi ...200817973155
decreased toxicity to terrestrial plants associated with a mixture of methyl tert-butyl ether and its metabolite tert-butyl alcohol.the influence of the main fuel oxygenate methyl tert-butyl ether (mtbe) and its key metabolite, tert-butyl alcohol (tba), on the growth of a plant seedling was studied separately and in combination. the test plants were mung bean (phaseolus radiatus), cucumber (cucumis sativus), wheat (triticum aestivum), sorghum (sorghum bicolor), kale (brassica alboglabra), chinese cabbage (brassica campestris), and sweet corn (zea mays). the growth of all the plants was adversely affected by tba and mtbe. the ...200717702346
municipal sludge-induced phytotoxicity.municipal sludge (ms), containing various types of environmental pollutants, can exert phytotoxic effects in plants. seed germination, chlorophyll content, plant weight, root and shoot growth, and metal levels, were measured as endpoints to assess the phytotoxic effects of leachates of ms from lucknow city in three plants, triticum aestivum, brassica campestris and phaseolus aureus. among the five metals analysed, lead was found to be present at the highest concentration, and chromium at the low ...200516268761
nitrogen-fixing pseudomonads isolated from roots of plants grown in the canadian high arctic.root-associated bacteria capable of reducing acetylene to ethylene (biological nitrogen fixation) were isolated from various native plants grown in the canadian high arctic. all the strains belonged to the genus pseudomonas but varied in several physiological characteristics. the rates of acetylene reduction at 14 or 20 degrees c were higher than at 25 or 9 degrees c. six strains reduced acetylene at 4 degrees c. all the strains exhibited chemotaxis to l-asparagine in semisolid agar at 4 to 25 d ...198616346983
ethnoveterinary practices for the treatment of parasitic diseases in livestock in cholistan desert (pakistan).this study was conducted to document the ethnoveterinary medicinal (evm) practices for the treatment of different parasitic diseases of livestock in cholistan desert, pakistan.200818524514
[effects of silicon on flowering chinese cabbage's anthracnose occurence, flower stalk formation, and silicon uptake and accumulation].different concentrations of silicon (si) were applied to flowering chinese cabbbage (brassica campestris l. ssp. chinensis var. utilis tsen et lee) to study their effects on the flowering chinese cabbage's anthracnose occurrence, flower stalk formation, and si uptake and accumulation. the results indicated that si could obviously control the occurrence of anthracnose, and the effect was genotype-dependant. the plants of susceptible cultivar applied with 2.5 mmol l(-1) si and those of resistant c ...200818655585
quadratic form: a robust metric for quantitative comparison of flow cytometric histograms.comparison of fluorescence distributions is a fundamental part of the analysis of flow cytometric data. this approach is applied to detect differences between control and test sample and thus analyze a biological response. comparison of standard test samples over time provides an estimate of instrument stability for quality control. however, application of statistical methods of distribution comparison in flow cytometry is difficult owing to instrument noise and the complex shape of intensity di ...200818561196
effects of bt transgenic chinese cabbage on the herbivore mamestra brassicae (lepidoptera: noctuidae) and its parasitoid microplitis mediator (hymenoptera: braconidae).we investigated the effects of a diamondback moth-resistant chinese cabbage (brassica campestris subsp napus variety pekinensis makino), expressing the insecticidal protein cryla(c) toxin derived from bacillus thuringiensis, on the nontarget herbivore mamestra brassicae (l.) (lepidoptera: noctuidae) and its parasitoid wasp microplitis mediator (haliday) (hymenoptera: braconidae). a decreased survival rate at neonate stage was observed in m. brassicae when reared on bt cabbage, although overall d ...200818767720
characterization of 1-aminocyclopropane-1-carboxylate (acc) deaminase containing methylobacterium oryzae and interactions with auxins and acc regulation of ethylene in canola (brassica campestris).the possible interaction of the plant hormones auxin and ethylene and the role of 1-aminocyclopropane-1-carboxylate (acc) deaminase containing bacteria on ethylene production in canola (brassica campestris) in the presence of inhibitory concentrations of growth regulators were investigated. the effects of auxin (indole-3-acetic acid and 2,4-dichlorophenoxy acetic acid), auxin transport inhibitor 2-(p-chlorophenoxy)-2-methylpropionic acid, ethylene precursor 1-aminocyclopropane-1-carboxylate and ...200717541630
interaction between plants and bacteria: glucosinolates and phyllospheric colonization of cruciferous vegetables by enterobacter radicincitans dsm 16656.for determining interactive plant-bacterial effects between glucosinolates and phyllospheric colonization by a plant growth-promoting strain, enterobacter radicincitans dsm 16656, in cruciferous vegetables, the extent of bacterial colonization was assessed in 5 cruciferous vegetables (brassica juncea, brassica campestris, brassica oleracea var. capitata, brassica rapa var. alboglabra, nasturtium officinale) using a species-specific taqman probe and quantitative real-time pcr. colonization abilit ...200919556746
ethanolic extracts of brassica campestris spp. rapa roots prevent high-fat diet-induced obesity via beta(3)-adrenergic regulation of white adipocyte lipolytic activity.the influence of ethanolic extracts of brassica campestris spp. rapa roots (ebr) on obesity was examined in imprinting control region (icr) mice fed a high-fat diet (hfd) and in 3t3-l1 adipocytes. the icr mice used were divided into regular diet, hfd, ebr (50 mg/kg/day ebr administered orally), and orlistat (10 mg/kg/day orlistat administered orally) groups. the molecular mechanism of the anti-obesity effect of ebr was investigated in 3t3-l1 adipocytes as well as in hfd-fed icr mice. in the obes ...201020132043
diffusion and accumulation in cultivated vegetable plants of di-(2-ethylhexyl) phthalate (dehp) from a plastic production factory.di-(2-ethylhexyl) phthalate (dehp) concentrations in the atmosphere and in four vegetable crops including brassica chinensis l. (bok choy), brassica campestris l. (field mustard), vigna unguiculata walp. (cowpea), and solanum melongena l. (eggplant) cultivated on land surrounding a plastic production factory were determined. the air dehp concentrations (means) at the sites 0.2, 0.4, 0.8, and 1.6 km away from the plastic production building were about 9.4-12.8, 5.8-9.6, 1.6-5.0, and 0.04-0.27 mic ...201020496185
the lcb2 subunit of the sphingolip biosynthesis enzyme serine palmitoyltransferase can function as an attenuator of the hypersensitive response and bax-induced cell death.previous results showed that expression of the gene encoding the long-chain base2 (lcb(2)) subunit of serine palmitoyltransferase (spt), designated bclcb(2), from nonheading chinese cabbage (brassica campestris ssp. chinensis) was up-regulated during hypersensitive cell death (hcd) induced by the phytophthora boehmeriae elicitor pb90. overexpression of bclcb(2) in nicotiana tabacum leaves suppressed the hcd normally initiated by elicitors and pb90-triggered h(2)o(2) accumulation. bclcb(2) also f ...200919076721
chinese cabbage (brassica campestris l.) does not improve glucose tolerance, serum insulin, or blood lipid profiles in a rat model of type-2 diabetes.the present study was conducted to investigate the effects of a low (0.5%) and a high (2.0%) dietary dose of freeze-dried chinese cabbage (cc) (brassica campestris l.) powder in a type-2 diabetes (t2d) model of rats. five-week-old male sprague-dawley rats were fed a high fat (hf)-containing diet for 2 wk then randomly divided into 4 groups of 8 animals, namely: normal control (nc), diabetic control (dbc), chinese cabbage low (ccl, 0.5%), and chinese cabbage high (cch, 2.0%) groups. diabetes was ...200819021803
isolation and characterization of juncin, an antifungal protein from seeds of japanese takana (brassica juncea var. integrifolia).an 18.9 kda antifungal protein designated juncin was isolated from seeds of the japanese takana (brassica juncea var. integrifolia). the purification protocol employed comprised anion-exchange chromatography on q-sepharose, affinity chromatography on affi-gel blue gel, cation exchange chromatography on sp-sepharose, and gel filtration on superdex 75. juncin was adsorbed on affi-gel blue gel and sp-sepharose but unadsorbed on q-sepharose. the protein exhibited antifungal activity toward the phyto ...200919354248
over-expression of putative transcriptional coactivator kelp interferes with tomato mosaic virus cell-to-cell movement.tomato mosaic virus (tomv) encodes a movement protein (mp) that is necessary for virus cell-to-cell movement. we have demonstrated previously that kelp, a putative transcriptional coactivator of arabidopsis thaliana, and its orthologue from brassica campestris can bind to tomv mp in vitro. in this study, we examined the effects of the transient over-expression of kelp on tomv infection and the intracellular localization of mp in nicotiana benthamiana, an experimental host of the virus. in co-bom ...200919236566
pedobacter rhizosphaerae sp. nov. and pedobacter soli sp. nov., isolated from the rhizosphere soil of chinese cabbage (brassica campestris).two bacterial strains 01-96(t) and 15-51(t) isolated from the rhizosphere soil of chinese cabbage (brassica campestris), were characterized using a polyphasic approach. the 16s rrna gene sequence analysis showed that strain 01-96(t) was phylogenetically related to pedobacter suwonensis 15-52(t) and pedobacter roseus 25b(t) (96.7 % and 96.7 % sequence similarity, respectively), and strain 15-51(t) was closely related to pedobacter borealis tfb(t) and pedobacter suwonensis 15-52(t) (97.2 % and 97. ...201121257686
effect of bacillus thuringiensis naturally colonising brassica campestris var. chinensis leaves on neonate larvae of pieris brassicae.the feeding of neonate larvae of pieris brassicae (order lepidoptera) on leaves of brassica plants that had been colonised by bacillus thuringiensis resulted in the death of 35% of the population within 72h. the bacteria multiplied in the cadavers, resulting in an increase of about 50-fold compared to the living insects. surviving insects showed no ill effects during the time of the study. there was negligible multiplication of b. thuringiensis in the frass.200919232351
variable intron content of the nadh dehydrogenase subunit 4 gene of plant mitochondria.the gene nad4, encoding subunit four of the mitochondrial nadh dehydrogenase complex i, has been isolated and characterized from turnip, brassica campestris. the 8 kb turnip nad4 gene contains four exons, which potentially encode a nad4 polypeptide of 495 amino acids, and three large group ii introns. northern analysis identifies an abundant 2 kb transcript that most likely serves as the nad4 mrna, while several larger transcripts (putative splicing intermediates) are also detected. analysis of ...19921525869
1,8-cineole inhibits both proliferation and elongation of by-2 cultured tobacco cells.volatile monoterpenes such as 1,8-cineole inhibit the growth of brassica campestris seedlings in a dose-dependent manner, and the growth-inhibitory effects are more severe for roots than hypocotyls. the preferential inhibition of root growth may be explained if the compounds inhibit cell proliferation more severely than cell elongation because root growth requires both elongation and proliferation of the constituent cells, whereas hypocotyl growth depends exclusively on elongation of existing ce ...201121344180
a novel method for constructing pathogen-regulated small rna cdna library.pathogen-responsive endogenous small non-coding rnas regulate gene expression in relation to plant immune responses by serving as rna silencing machinery. decay caused by the bacterium, erwinia carotovora subsp. carotovora (ecc), often leads to soft rot disease in the plant brassica campestris l. ssp. pekinensis (bcp). to discover endogenous small rna species in bcp in response to ecc infection, we developed a highly efficient approach for cloning pathogen-regulated small rnas. a group of degene ...201020515661
[the pta29-barnase chimeric gene transformation of brassica campestris l. subsp. chinensis makino var. parachinensis mediated by agrobacterium].in order to induce male sterility of brassica campestris l. subsp. chinensis makino var. parachinensis, we introduced the chimeric pta29-barnase gene into it by agrobacteriumtume faciens transformation. we obtained the transgenic plants, and determined them by pcr, southern blotting and rt-pcr analysis. results indicated that the rnase (barnase) gene had been transferred into genome of plant, and its expression level was different among transformation plants. all transgenic plants were male ster ...200818724712
bioaccumulation and translocation of cadmium in cole (brassica campestris l.) and celery (apium graveolens) grown in the polluted oasis soil, northwest of china.a pot experiment was conducted to study the bioaccumulation and translocation of cadmium (cd) in cole (brassica campestris l.) and celery (apium graveolens) grown in the cd-polluted oasis soil, northwest of china. the results showed that cd in the unpolluted oasis soil was mainly bound to carbonate fraction (f2) and fe-mn oxide fraction (f3). however, marked change of cd fractions was observed with increasing soil cd concentrations, in which the concentration of cd in fl (exchangeable fraction), ...201122128545
identification and preliminary analysis of a new pcp promoter from brassica rapa ssp. chinensis.the promoter of brassica campestris male fertile 5 (bcmf5), a pollen coat protein member, class a (pcp-a) gene family, was isolated from brassica rapa l. ssp. chinensis makino (chinese cabbage-pak-choi) by thermal asymmetric interlaced polymerase chain reaction (tail-pcr). sequence analysis suggested that the 605-bp promoter of bcmf5 appears to be a pollen promoter. in an attempt to confirm the promoter activity of bcmf5 promoter, -609 to +3 bp and -377 to +3 bp fragments of the upstream sequenc ...200817851779
influence of various host plants on the consumption and utilization of food by pieris brassicae (linn.).pieris brassicae (linn.) is a destructive cosmopolitan pest of cruciferous crops. it is present wherever its host plants occur, and it is considered to be one of the most widely distributed of all the lepidoptera. we investigated the affect of various host plants on the food consumption and utilization by p. brassicae. we quantified consumption of food, larval duration, pupal duration and weight on cabbage (brassica oleracea var. capitata), cauliflower (brassica oleracea var. botrytis), radish ( ...201122030306
toxicities of fipronil enantiomers to the honeybee apis mellifera l. and enantiomeric compositions of fipronil in honey plant flowers.fipronil is a chiral phenylpyrazole insecticide that is effective for control of a wide range of agricultural and domestic pests at low application rates. wide application of fipronil also causes poisoning of some nontarget insects, such as honeybees. in the present study, toxicities of fipronil enantiomers and racemate to the honeybee apis mellifera l. were determined to examine whether using formulations of single or enriched fipronil enantiomer is a possible option to reduce risks to bees. co ...201020821427
agrobacterium-mediated transformation and regeneration of fertile transgenic plants of chinese cabbage (brassica campestris ssp. pekinensis cv. 'spring flavor').a procedure for the regeneration of fertile transgenic chinese cabbage (brassica campestris ssp. pekinensis cv. 'spring flavor') is presented in this report. the protocol is based on infection of cotyledon explants of 5-d-old seedlings with an agrobacterium tumefaciens strain lba4404 carrying a disarmed binary vector ptok/bks-1. the t-dna region of this binary vector contains the nopaline synthase/neomycin phosphotransferase ii (nptii) chimeric gene for kanamycin resistance and the cauliflower m ...199524194308
the effects of acetosyringone and ph on agrobacterium-mediated transformation vary according to plant species.expiants of five plant species (allium cepa, antirrhinum majus, brassica campestris. glycine max, and nicotiana tabacum) were co-cultivated with three agrobacterium tumefaciens strains under different conditions to assess the effects of acetosyringone and medium ph on strain virulence. tumours were incited on all dicotyledonous species by strains n2/73 and a281. the presence of acetosyringone during co-cultivation generally enhanced the virulence of these strains, most markedly n2/73 on a. majus ...199124213690
[allelopathic effects of streptomyces sp. 6803 on plants].streptomyces can produce an overwhelming majority of known antibiotics and several biologically active compounds, but whether streptomyces can display allelopathic effects on higher plants is largely unknown. in this study, seven actinomyces strains isolated from soils showed inhibitory effect on plant seedlings growth, among which, streptomyces sp. 6803 had strong capability in inhibiting the seedlings growth of brassica campestris and echinochloa crusgalli in both solid and liquid cultures. th ...201223359933
evaluation of rhizospheric pseudomonas and bacillus as biocontrol tool for xanthomonas campestris pv campestris.xanthomonas campestris pv campestris (xcc), causing black rot, is one of the most yield-limiting and destructive pathogens of cruciferous crops. the intention of this study was to evaluate the potential of rhizobacteria in black rot management. fifty-four isolates from rhizosphere soil of brassica campestris were screened against xcc. two isolates namely, ka19 and se, with inhibition radius >11 mm were selected. the combined use of them produced an average inhibition zone of 18.1 ± 1.4 mm radius ...201222806865
the use of lacz marker in enumeration of azotobacter chroococcum in carrier based inoculants.a transconjugant of azotobacter chroococcum mac 27 tagged with lac z(a. chroococcum mac27 l) was found to possess high levels of β-galactosidase activity constitutively. further, the lac z marker was found to be stably integrated into the chromosome of the a. chroococcum mac 27 and did not have any adverse effect on growth, nitrogen fixation and excretion of ammonia. a quick method to determine the viable cell number in broth culture and carrier based inoculants has been developed on the basis o ...201425242946
soil and vegetables enrichment with heavy metals from geological sources in gilgit, northern pakistan.this study was conducted to investigate the concentrations of heavy metals in soil and vegetables, and human health risks through ingestion of contaminated vegetables. soil and vegetable samples were collected from different locations in gilgit, northern pakistan, and analyzed for cd, cu, ni, pb and zn. plant transfer factors (ptf), daily intake of metals (dim) and health risk index (hri) were also calculated. the concentrations of cd, cu and zn exceeded their respective permissible limits in so ...201020810165
bcpmi2, isolated from non-heading chinese cabbage encoding phosphomannose isomerase, improves stress tolerance in transgenic tobacco.phosphomannose isomerase (pmi) is an enzyme that catalyses the first step of the l-galactose pathway for ascorbic acid (asa) biosynthesis in plants. to clarify the physiological roles of pmi in asa biosynthesis, the cdna sequence of pmi was cloned from non-heading chinese cabbage (brassica campestris ssp. chinensis makino) and overexpressed in tobacco transformed with agrobacterium tumefaciens. the asa and soluble sugar contents were lower in 35s::bcpmi2 tobacco than in wild-type tobacco. howeve ...201424430300
protective effect of the cruciferous vegetable mustard leaf (brassica campestris) against in vivo chromosomal damage and oxidative stress induced by gamma-radiation and genotoxic chemicals.we evaluated the possible protective effect of the popular indian cruciferous vegetable mustard leaf (brassica campestris) against chromosomal damage and oxidative stress induced by gamma-radiation, cyclophosphamide (cph) and urethane (ure), in mice. in vivo bone marrow micronucleus test was performed to assess chromosomal damage, and oxidative stress was monitored by estimating the changes in lipid peroxidation and the status of glutathione (gsh) as well as redox cycle antioxidants. pretreatmen ...200818418865
A novel cold-inducible gene from Pak-choi (Brassica campestris ssp. chinensis), BcWRKY46, enhances the cold, salt and dehydration stress tolerance in transgenic tobacco.WRKY TFs belong to one of the largest families of transcriptional regulators in plants and form integral parts of signaling webs that modulate many plant processes. BcWRKY46, a cDNA clone encoding a polypeptide of 284 amino acids and exhibited the structural features of group III of WRKY protein family, was isolated from the cold-treated leaves of Pak-choi (Brassica campestris ssp. chinensis Makino, syn. B. rapa ssp. chinensis) using the cDNA-AFLP technique. Expression of this gene was induced q ...201121938429
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