Publications

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the dynamic behaviour of photoreceptor cells in the fly in response to random (white noise) stimulation at a range of temperatures.1. photoreceptor cells in calliphora stygia were stimulated with randomly fluctuating green light while the resulting fluctuations in membrane potential were recorded with intracellular micro-electrodes. 2. fourier analysis was used to obtain the frequency response functions between the light intensity fluctuations and the membrane potential fluctuations at a range of different temperatures. 3. the results show that for small light fluctuations the transducer function can be modelled by a cascad ...1978624996
metabolism of 3beta,14alpha-dihydroxy-5beta-(3alpha-3h)-cholest-7-en-6-one in calliphora stygia. 19751120911
detection of ige antibodies to a wide range of insect species in subjects with suspected inhalant allergies to insects.sera obtained from subjects diagnosed on the basis of case history and skin tests as having inhalant allergies to insects, were tested for the presence of ige antibodies to antigens from the house fly musca domestica, a blowfly calliphora stygia, the common clothes moth tineola bisselliella, warehouse moth ephestia cautella, cockroach blattella germanica, carpet beetle anthrenus verbasci and silverfish ctenolepisma longicaudata. approximately one-third of the sera reacted with extracts from all ...19883350607
developmental changes in the major inclusion bodies of polytene nuclei from larval tissues of the blowfly, calliphora stygia. 19704193397
metabolism of some possible ecdysone precursors in calliphora stygia. 19744436587
developmental changes in the late larva of calliphora stygia. i. haemolymph. 19684882875
developmental changes in the late larva of calliphora stygia. ii. protein synthesis. 19695374537
developmental changes in the late larva of calliphora stygia. 3. the occurrence and synthesis of specific tissue proteins. 19715579443
developmental changes in the late larva of calliphora stygia. iv. uptake of plasma protein by the fat body. 19715579444
ultrastructure of probable sites of release of neurosecretory materials in an insect, calliphora stygia fabr. (diptera). 19665946403
a model for the aging of fly larvae in forensic entomology.a method is described for determining the time of hatching of blowfly larvae on a corpse with respect to temperature. temperature is known to have a profound effect on the rate of growth of fly larvae, and it is suggested that past assumptions that the growth of larvae found in a corpse has taken place at one approximated ambient temperature may have led to large overestimates of the development time of the larvae, and thus overestimates of time of death. larvae of lucilia cuprina, calliphora vi ...19846745822
habitat preferences and carcase colonization by sheep blowflies in the northern north island of new zealand.in urban auckland, from september 1991 to may 1992, only six specimens of the australian sheep blowfly, lucilia cuprina, were collected in farmed parkland and in garden habitats. the common green blowfly, lucilia sericata, was the most common calliphorid trapped in these habitats. neither of the two lucilia species were found in native bush remnants in urban or rural areas where calliphora hilli was dominant. similarly very few l.cuprina (0.3% of the total) were trapped in rural rubbish tips in ...19938481532
the forensic application of allozyme electrophoresis to the identification of blowfly larvae (diptera: calliphoridae) in southern australia.most known carrion-breeding species of blowflies in southern australia are of the genus calliphora. the morphological similarity of the immatures of these species means that correctly identifying them poses a challenge for forensic entomologists. this study investigates the potential of allozyme analysis to assist with this task. molecular profiles of third-instar larvae and adults representing four of these carrion-breeding species, calliphora stygia, c. dubia, c. hilli hilli, and c. vicina, we ...200111373007
food consumption and individual lifespan of adults of the blowfly, calliphora stygia: a test of the 'rate of living' theory of aging.following eclosion, adult calliphora stygia were individually housed (at 22 degrees c and 73% rh) in 125 ml plastic vials and provided with ad libitum access to either 0.125 or 0.2m sucrose as a food source and daily food consumption measured throughout their adult life. all blowflies were weighed daily and food consumed is determined by weighing individual food dishes. blowflies provided with 0.125 m sucrose (n=59) consumed daily a significantly greater amount of the sucrose solution than those ...200415501018
the determination of morphine in the larvae of calliphora stygia using flow injection analysis and hplc with chemiluminescence detection.selective determination of morphine in the larvae of calliphora stygia (fabricius) (diptera: calliphoridae) using acidic potassium permanganate chemiluminescence detection coupled with flow injection analysis and high-performance liquid chromatography (hplc) is described. larvae of c. stygia were reared on minced meat substrates that had been spiked with varying concentrations of morphine. morphine concentrations were chosen to reflect typical levels in human tissues from opiate overdose victims ...200617132246
blowfly succession from possum (trichosurus vulpecula) carrion in a sheep-farming zone.the significance of brushtail possum, trichosurus vulpecula kerr (diprotodontia: phalangeridae) carcasses to the succession and production of diptera species and its relevance to fly strike management in tasmania, australia was examined. calliphora stygia (fabricius), lucilia sericata (meigen) and calliphora vicina robineau-desvoidy (diptera: calliphoridae) were found to be the most abundant and lucilia cuprina (wiedemann) (diptera: calliphoridae) always the least abundant (< 1%) of the putative ...200617199756
a preliminary investigation of insect colonisation and succession on remains in new zealand.a preliminary, systematic field study on the process of decomposition and associated insects was conducted, for the first time, in new zealand. using pig carcasses as an animal model for human decomposition, insect colonisation and succession was monitored in three different habitats in the auckland region where remains are likely to be found. a significant difference in the rates of decomposition was found among the three different habitats of an open field, coastal sand dune area and native bu ...200817997065
a global study of forensically significant calliphorids: implications for identification.a proliferation of molecular studies of the forensically significant calliphoridae in the last decade has seen molecule-based identification of immature and damaged specimens become a routine complement to traditional morphological identification as a preliminary to the accurate estimation of post-mortem intervals (pmi), which depends on the use of species-specific developmental data. published molecular studies have tended to focus on generating data for geographically localised communities of ...200818295422
experimental studies of blowfly (calliphora stygia) longevity: a little dietary fat is beneficial but too much is detrimental.this paper is one in a series of experimental studies on the effects of food composition on aging and longevity, using the golden-haired blowfly calliphora stygia as the animal model. here we examine how diet fat content affects blowfly life history traits such as longevity, reproduction, feeding rate, body mass, total fat content and membrane fatty acid composition. the highest median and maximum longevity was observed in blowflies fed on low fat diets, while high-fat diets caused more rapid de ...200919632351
effect of morphine on the growth rate of calliphora stygia (fabricius) (diptera: calliphoridae) and possible implications for forensic entomology.insect specimens collected from decomposing bodies enable forensic entomologists to estimate the minimum post-mortem interval (pmi). drugs and toxins within a corpse may affect the development rate of insects that feed on them and it is vital to quantify these effects to accurately calculate minimum pmi. this study investigated the effects of morphine on growth rates of the native australian blowfly, calliphora stygia (fabricius) (diptera: calliphoridae). several morphine concentrations were inc ...200919773137
accumulation and excretion of morphine by calliphora stygia, an australian blow fly species of forensic importance.this study examined the ability of the forensically important blow fly, calliphora stygia to actively excrete morphine, thereby maintaining a low morphine level within its body when fed on a diet containing morphine at low (7pmolg(-1)) and high (17.5pmolg(-1)) concentrations. morphine was accumulated within the bodies of maggots (≈70% within the tissues) at concentrations which were lower than that of the meat (3-24%). the morphine content of the initial developing stages (second and third insta ...201020888829
chemosensory genes identified in the antennal transcriptome of the blowfly calliphora stygia.blowflies have relevance in areas of forensic science, agriculture, and medicine, primarily due to the ability of their larvae to develop on flesh. while it is widely accepted that blowflies rely heavily on olfaction for identifying and locating hosts, there is limited research regarding the underlying molecular mechanisms. using next generation sequencing (illumina), this research examined the antennal transcriptome of calliphora stygia (fabricius) (diptera: calliphoridae) to identify members o ...201525880816
effects of methamphetamine and its primary human metabolite, p-hydroxymethamphetamine, on the development of the australian blowfly calliphora stygia.the larvae of necrophagous fly species are used as forensic tools for the determination of the minimum postmortem interval (pmi). however, any ingested drugs in corpses may affect larval development, thus leading to incorrect estimates of the period of infestation. this study investigated the effects of methamphetamine and its metabolite, p-hydroxymethamphetamine, on the forensically important australian blowfly calliphora stygia. it was found that the presence of the drugs significantly acceler ...201424905152
diet fatty acid profile, membrane composition and lifespan: an experimental study using the blowfly (calliphora stygia).the membrane pacemaker theory of ageing proposes that the polyunsaturated fatty acid (pufa) composition of membrane lipids of a species is an important determinant of its maximum lifespan. we report three experiments using the blowfly calliphora stygia, where this theory was tested by manipulation of dietary fat profile. although the fat profile of the larval diet resulted in small alterations of individual membrane fatty acids, it had no effect on the peroxidation index (pi) of membrane lipids ...201424631404
effect of temperature on the rate of ageing: an experimental study of the blowfly calliphora stygia.all organisms age, the rate of which can be measured by demographic analysis of mortality rates. the rate of ageing is thermally sensitive in ectothermic invertebrates and we examined the effects of temperature on both demographic rates of ageing and on cellular senescence in the blowfly, calliphora stygia. the short lifespan of these flies is advantageous for demographic measurements while their large body size permits individual-based biochemical characterisation. blowflies maintained at tempe ...201324019937
examination of forensic entomology evidence using computed tomography scanning: case studies and refinement of techniques for estimating maggot mass volumes in bodies.a new technique has recently been developed for estimating the volume of maggot masses on deceased persons using post-mortem ct scans. this allows volume to be measured non-invasively and factored into maggot mass temperature calculations for both casework and research. examination of admission scans also allows exploration of entomological evidence in anatomical areas not usually exposed by autopsy (e.g. nasal cavities and facial sinuses), and before autopsy disrupts the maggot distribution on ...201222639347
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