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[experimental inoculation of the chick embryo with sphaerophorus funduliformis]. 196113721378
endotoxin-triggered haematological interactions in fusobacterium necrophorum infections.the haematological mechanisms in the course of liver abscess formation were evaluated. they were examined by employing viable cells of fusobacterium necrophorum subsp. necrophorum and fusobacterium necrophorum subsp. funduliforme in comparison with their endotoxins. whole cell infection with f.n. necrophorum led to neutrophilia and to a concomitant monocytosis in parallel with those responses induced by the in vivo injection of its endotoxin. viable infection with f.n. funduliforme was character ...200010817519
comparison of haemolytic activity between fusobacterium necrophorum subsp. necrophorum and fusobacterium necrophorum subsp. funduliforme in vitro and in vivo.haemolytic activity of two subspecies of fusobacterium necrophorum was compared in vitro and in vivo. f. necrophorum subsp. necrophorum (fnn) showed a stronger activity than f. necrophorum subsp. funduliforme (fnf) in vitro. haemolytic activity of fnn and fnf was 57.97%+/-1.90 and 17.33%+/-1.44, respectively, compared to complete haemolysis by distilled water. in the mice injected with fnn, haemolysin was detected in the liver at a titre of from 1 : 16 to 1 : 128, and fnn was recovered from all ...19968645462
ribotyping to differentiate fusobacterium necrophorum subsp. necrophorum and f. necrophorum subsp. funduliforme isolated from bovine ruminal contents and liver abscesses.differences in biological activities (hemagglutination, hemolytic, leukotoxic, and virulence) and ribotypes between the two subspecies of fusobacterium necrophorum of bovine ruminal and liver abscess origins were investigated. hemagglutination activity was present in all hepatic, but only some ruminal, strains of fusobacterium necrophorum subsp. necrophorum. ruminal f. necrophorum subsp. necrophorum had low leukotoxin titers yet was virulent in mice. fusobacterium necrophorum subsp. funduliforme ...19968593050
[clinical and bacteriological study of bacteroides infections. report on 10 clinical observations of sphaerophorus funduliformis and one of ristella pseudoinsolita]. 19715143574
chemical investigations on the endotoxins of sphaerophorus funduliformis. 19734786202
proposal of two subspecies of fusobacterium necrophorum (flügge) moore and holdeman: fusobacterium necrophorum subsp. necrophorum subsp. nov., nom. rev. (ex flügge 1886), and fusobacterium necrophorum subsp. funduliforme subsp. nov., nom. rev. (ex hallé 1898).the biological and biochemical properties, dna base compositions, and levels of dna-dna homology of two biovars of fusobacterium necrophorum were examined. some differences were found between the two biovars in biological and biochemical properties. the g + c contents of dnas from biovar a strains vpi 2891t (t = type strain), nctc 10576, n167, fn47, and fn43, were 32, 30, 29, 28, and 31 mol%, respectively. the g + c contents of dnas from biovar b strains fn524t, 606, fn49, fn45, and 1260 were 30 ...19911883714
pathogenicity of fusobacterium necrophorum biovar b.previous studies showed that the high minimum infective dose (more than 10(6) organisms) of biovar a strains of fusobacterium necrophorum for mice by subcutaneous inoculation could be greatly reduced, often to less than 10 organisms, by suspending the fusobacteria in sublethal doses of broth cultures of certain other bacterial species, such as staphylococcus aureus. the present study showed that no such enhancement of infectivity occurred with two biovar b strains. this observation, together wit ...19921585082
comparative bacteriological studies on summer mastitis in grazing cattle and pyogenes mastitis in stabled cattle in denmark.a total of 143 secretions from clinical cases of summer mastitis (sm) in grazing cattle and from 89 cases of pyogenes mastitis (pm) in stabled cattle were examined bacteriologically. the typical bacteriological finding was a mixed flora in which the predominant organisms were actinomyces pyogenes (sm-70%, pm-85%), peptostreptococcus indolicus (54%, 54%), a microaerophilic coccus (stuart-schwan coccus) (26%, 25%), fusobacterium necrophorum biovar b (22%, 12%), bacteroides melaninogenicus (20%, 9% ...19921355316
immunochemical investigations on sphaerophorus funduliformis endotoxins. 19751212626
stability and stabilization of fusobacterium necrophorum hemolysin.the stability and stabilization of the hemolytic activity of fusobacterium necrophorum subsp. necrophorum and fusobacterium necrophorum subsp. funduliforme were monitored over a period of four weeks using culture supernatants. the hemolytic activity was completely lost after one week at room temperature and 37 degrees c. after a two-week storage at 4 degrees c and -80 degrees c only trace activity was detected with -80 degrees c being the better of the two conditions. the addition of cysteine mo ...19968810016
serological investigations on endotoxins isolated from sphaerophorus funduliformis (syn. fusobacterium necrophorum) strains. 19744217645
tryptophanase from sphaerophorus funduliformis. purification, molecular weight and subunit properties.a crystalline tryptophanase can be obtained from extracts of spaerophorus funduliformis using a heat treatment, hydroxyapatite chromatography and solubility in solutions of (nh4)2so4 as a function of ph and temperature. the purified enzyme is homogeneous by several criteria. s. funduliformis tryptophanase has a specific activity of 11.5-13.5 and requires pyridoxal 5'-phosphate for enzymatic activity. like other tryptophanases that have been studied, the s. funduliformis enzyme is a tetramer prot ...1975236027
[observations concerning 7 strains of saphaerophorus necrophorus, sphaerophorus funduliformis, and sphaerophorus pseudonecrophorus species]. 19714939845
comparison of extracellular enzymes of fusobacterium necrophorum subsp. necrophorum and fusobacterium necrophorum subsp. funduliforme.a total of 10 strains each of fusobacterium necrophorum subsp. necrophorum and fusobacterium necrophorum subsp. funduliforme were tested for the production of 13 extracellular enzymes. dnase, alkaline phosphatase, and lipase were predominantly associated with all the strains of f. necrophorum subsp. necrophorum, with dnase not detected in any of the strains of f. necrophorum subsp. funduliforme. in addition, the strains of f. necrophorum subsp. necrophorum were generally more hemolytic than thos ...19938370761
detection of fusobacterium necrophorum subsp. funduliforme in tonsillitis in young adults by real-time pcr.throat swabs from 61 patients, aged 18-32 years, with non-streptococcal tonsillitis (nst) and 92 healthy controls were examined for the presence of fusobacterium necrophorum dna using a novel taqman-based real-time quantitative pcr assay for f. necrophorum subspecies. the assay was based on the gyrb subunit gene, and detected f. necrophorum dna in 48% of patients with nst and in 21% of controls (p <0.001). f. necrophorum subsp. funduliforme was the only subspecies found in both patients and cont ...200717403128
fusobacterium necrophorum- detection and identification on a selective agar.within the last decade, fusobacterium necrophorum subsp. funduliforme has been considered a clinically important pathogen causing pharyngitis especially in adolescents and young adults. f. necrophorum pharyngitis can progress into lemierre's syndrome, which is a severe and life-threatening infection. however, throat swabs are not cultured anaerobically in the routine and even if cultured anaerobically, it can be difficult to identify f. necrophorum from the normal flora of the throat. f. necroph ...201021091782
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