Publications

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hematologic values of captive mexican wolves.hematologic reference values were determined for a captive population of 11 mexican wolves (canis lupus baileyi). wolf pups from 4 to 24 weeks old had progressive age-related increases in pcv, hemoglobin concentration, mean cell volume, and rbc counts similar to those seen in domestic dog pups (c familiaris). hematologic indices in wolves older than 24 weeks were comparable to those of the adult domestic dog; however, pcv, hemoglobin concentration, and rbc counts were higher.19911785734
canine parvovirus enteritis, canine distemper, and major histocompatibility complex genetic variation in mexican wolves.the endangered mexican wolf (canis lupus baileyi) was recently reintroduced into arizona and new mexico (usa). in 1999 and 2000, pups from three litters that were part of the reintroduction program died of either canine parvovirus or canine distemper. overall, half (seven of 14) of the pups died of either canine parvovirus or canine distemper. the parents and their litters were analyzed for variation at the class ii major histocompatibility complex (mhc) gene drb1. similar mhc genes are related ...200314733289
influence of cooling rates and addition of equex pasta on cooled and frozen-thawed semen of generic gray (canis lupus) and mexican gray wolves (c. l. baileyi).a current priority for the preservation of the endangered mexican gray wolf (canis lupus baileyi) is the development of a sperm-based genome resource bank for subsequent use in artificial insemination. to optimize the quality of cryopreserved sperm, the procedures involved in processing semen before and during freezing need to be improved. the aim of this study were to examine the effects of: (i) different cooling periods before freezing and (ii) addition of equex pasta (minitüb, tübingen, germa ...200616472849
artificial insemination in canids: a useful tool in breeding and conservation.artificial insemination (ai) and semen freezing have become services available to dog owners worldwide, and the demand for services to freeze semen is increasing. in other canids such as the fox, the fur industry utilizes fresh or frozen semen to artificially inseminate vixens to produce pelts. clearly, ai facilitates the use of a male to sire several females by diluting the ejaculate, increases breeding hygiene, and allows crossing between species with slightly different breeding seasons. the a ...200918947865
vitrification of oocytes from endangered mexican gray wolves (canis lupus baileyi).careful genetic management, including cryopreservation of genetic material, is central to conservation of the endangered mexican gray wolf. we tested a technique, previously used to vitrify human and domestic animal oocytes, on oocytes from domestic dogs as a model and from the endangered mexican wolf. this method provided a way to conserve oocytes from genetically valuable older female mexican wolves as an alternative to embryos for preserving female genes. oocytes were aspirated from ovaries o ...201021111469
intestinal rupture in a mexican gray wolf (canis lupus baileyi) in association with ingestion of a diazinon-impregnated cattle ear tag.a free-ranging mexican gray wolf (canis lupus baileyi) suffered intestinal rupture following ingestion of an insecticidal cattle ear tag. subsequent organophosphate toxicosis as a cause of the rupture was speculated. insecticidal ear tags could represent a poisoning risk in canids and other wildlife scavengers.201323307392
applying clinically proven human techniques for contraception and fertility to endangered species and zoo animals: a review.reversible contraception that does not alter natural behavior is a critical need for managing zoo populations. in addition to reversible contraception, other fertility techniques perfected in humans may be useful, such as in vitro fertilization (ivf) or oocyte and embryo banking for endangered species like amphibians and mexican wolves (canis lupus baileyi). furthermore, the genetics of human fertility can give a better understanding of fertility in more exotic species. collaborations were estab ...201324437091
developing metapopulation connectivity criteria from genetic and habitat data to recover the endangered mexican wolf.restoring connectivity between fragmented populations is an important tool for alleviating genetic threats to endangered species. yet recovery plans typically lack quantitative criteria for ensuring such population connectivity. we demonstrate how models that integrate habitat, genetic, and demographic data can be used to develop connectivity criteria for the endangered mexican wolf (canis lupus baileyi), which is currently being restored to the wild from a captive population descended from 7 fo ...201424112074
genetic rescue and inbreeding depression in mexican wolves.although inbreeding can reduce individual fitness and contribute to population extinction, gene flow between inbred but unrelated populations may overcome these effects. among extant mexican wolves (canis lupus baileyi), inbreeding had reduced genetic diversity and potentially lowered fitness, and as a result, three unrelated captive wolf lineages were merged beginning in 1995. we examined the effect of inbreeding and the merging of the founding lineages on three fitness traits in the captive po ...200717609180
gnrh-agonist induction of fertile estrus with either natural mating or artificial insemination, followed by birth of pups in gray wolves (canis lupus).although captive populations of endangered species such as the mexican gray wolf (canis lupus baileyi) can benefit from artificial insemination to accomplish genetic exchange, reliable techniques for timing insemination are lacking. we used the generic gray wolf (c. lupus) to test the efficacy of a short-acting gnrh-agonist implant, deslorelin, for inducing estrus. of five females receiving implants on 17 or 18 january 2003, two mated naturally 10-17 days later, and the others were artificially ...200616469367
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