| reclassification of amycolatopsis rugosa lechevalier et al. 1986 as prauserella rugosa gen. nov., comb. nov. | an almost complete sequence of the 16s rdna of the type strain of amycolatopsis rugosa was determined following direct sequencing of the amplified gene. the sequence was aligned with those of representatives of the family pseudonocardiaceae and related actinomycetes and phylogenetic trees were inferred by using three tree-making algorithms. the organism formed a distinct clade within the evolutionary radiation occupied by the family pseudonocardiaceae. it was also readily distinguished from all ... | 1999 | 10319471 |
| functional analysis of alkane hydroxylases from gram-negative and gram-positive bacteria. | we have cloned homologs of the pseudomonas putida gpo1 alkane hydroxylase from pseudomonas aeruginosa pao1, pseudomonas fluorescens cha0, alcanivorax borkumensis ap1, mycobacterium tuberculosis h37rv, and prauserella rugosa nrrl b-2295. sequence comparisons show that the level of protein sequence identity between the homologs is as low as 35%, and that the pseudomonas alkane hydroxylases are as distantly related to each other as to the remaining alkane hydroxylases. based on the observation that ... | 2002 | 11872725 |
| prauserella halophila sp. nov. and prauserella alba sp. nov., moderately halophilic actinomycetes from saline soil. | the actinomycete strains yim 90001(t) and yim 90005(t) were isolated on starch-casein agar [20 % (w/v) nacl, ph 7.0] from a soil sample collected from xinjiang province in the west of china. the two isolates were aerobic and gram-positive. the substrate mycelium was fragmented and an aerial mycelium was well developed on czapek medium. the aerial mycelium formed long spore chains with branched short or long spore chains at maturity, which were straight to flexuous, and spores were non-motile. ba ... | 2003 | 13130045 |
| new alk genes detected in antarctic marine sediments. | alkane monooxygenases (alk) are the key enzymes for alkane degradation. in order to understand the dispersion and diversity of alk genes in antarctic marine environments, this study analysed by clone libraries the presence and diversity of alk genes (alkb and alkm) in sediments from admiralty bay, king george island, peninsula antarctica. the results show a differential distribution of alk genes between the sites, and the predominant presence of new alk genes, mainly in the pristine site. sequen ... | 2009 | 19207566 |
| prauserella salsuginis sp. nov., prauserella flava sp. nov., prauserella aidingensis sp. nov. and prauserella sediminis sp. nov., isolated from a salt lake. | strains yim 90625(t), yim 90630(t), yim 90636(t) and yim 90694(t) were isolated from a salt lake in xinjiang province, north-west china, and were subjected to a polyphasic analysis to determine their taxonomic positions. all isolates were moderately halophilic and were able to grow at nacl concentrations up to 15 or 20 % (w/v). the genomic dna g+c contents of the strains ranged from 69.1 to 70.1 mol%. based on the results of 16s rrna gene sequence analysis, dna-dna relatedness, phenotypic charac ... | 2009 | 19628605 |
| prauserella muralis sp. nov., from the indoor environment. | a novel gram-stain-positive, mycelium-forming actinobacterium (05-be-005(t)) isolated from the wall of an indoor environment was studied for its taxonomic position. the isolated strain formed a substrate mycelium that fragmented into rod-shaped cells and showed an aerial mycelium on medium m79. on the basis of 16s rrna gene sequence similarity studies, strain 05-be-005(t) was shown to belong to the genus prauserella, closely related to prauserella rugosa dsm 43194(t) (96.6 % similarity), prauser ... | 2010 | 19651719 |
| prauserella marina sp. nov., isolated from ocean sediment of the south china sea. | a novel actinomycete strain, designated ms498(t), was isolated from an ocean sediment sample collected from the south china sea. it was subjected to a polyphasic analysis to determine its taxonomic position. the phylogenetic tree based on 16s rrna gene sequences showed that strain ms498(t) had the highest similarity (96.5 %) with members of the genus prauserella and was loosely associated with prauserella rugosa dsm 43194(t) and saccharomonospora halophila dsm 44411(t). based on 16s rrna gene se ... | 2010 | 19666793 |
| yuhushiella deserti gen. nov., sp. nov., a new member of the suborder pseudonocardineae. | a thermotolerant, gram-stain-positive, aerobic, sporangium-forming actinomycete, strain ra45(t), was isolated from a desert region in xinjiang uigur autonomous region, north-western china. comparative analysis of the 16s rrna gene sequence and phenotypic characterization revealed that strain ra45(t) belonged phylogenetically to the family pseudonocardiaceae of the suborder pseudonocardineae. strain ra45(t) showed more than 5 % 16s rrna gene sequence divergence from recognized species of genera ... | 2011 | 20400669 |
| [cloning and characterization of gene cluster for biosynthesis of ectoine and 5-hydroxyectoine from extreme halotolerant actinomycete strain prauserella alba yim 90005(t)]. | to study adaptive mechanism in hypersaline environments of extreme halotolerant filamentous actinomycetes. | 2011 | 21800621 |