two new subspecies of photorhabdus luminescens, isolated from heterorhabditis bacteriophora (nematoda: heterorhabditidae): photorhabdus luminescens subsp. kayaii subsp. nov. and photorhabdus luminescens subsp. thracensis subsp. nov.bacterial isolates from nematodes from turkish soil samples were initially characterized by molecular methods and seven members of the genus photorhabdus identified to the species level, using riboprint analyses and metabolic properties. strain 07-5 (dsm 15195) was highly related to the type strain of photorhabdus luminescens subsp. laumondii dsm 15139t, and was regarded a strain of this subspecies. strains 1121t (dsm 15194t), 68-3 (dsm 15198) and 47-10 (dsm 15197) formed one, strain 39-8t (dsm ...200415053319
cloning serratia entomophila antifeeding genes--a putative defective prophage active against the grass grub costelytra zealandica.serratia entomophila and serratia proteamaculans (enterobacteriaceae) cause amber disease in the grass grub costelytra zealandica (coleoptera: scarabaeidae), an important pasture pest in new zealand. larval disease symptoms include cessation of feeding, clearance of the gut, amber coloration, and eventual death. a 155-kb plasmid, padap, carries the genes sepa, sepb, and sepc, which are essential for production of amber disease symptoms. transposon insertions in any of the sep genes in padap abol ...200415262948
cloning and sequencing of a genomic island found in the brazilian purpuric fever clone of haemophilus influenzae biogroup aegyptius.a genomic island was identified in the haemophilus influenzae biogroup aegyptius brazilian purpuric fever (bpf) strain f3031. this island, which was also found in other bpf isolates, could not be detected in non-bpf biogroup aegyptius strains or in nontypeable or typeable h. influenzae strains, with the exception of a region present in the type b eagan strain. this 34,378-bp island is inserted, in reference to h. influenzae rd kw20, within a choline transport gene and contains a mosaic structure ...200515784532
the gene stla encodes a phenylalanine ammonia-lyase that is involved in the production of a stilbene antibiotic in photorhabdus luminescens tt01.photorhabdus is a genus of gram-negative bacteria from the family enterobacteriaceae. members of photorhabdus have a complex life cycle during which the bacterium has a pathogenic interaction with insect larvae whilst also maintaining a mutualistic relationship with nematodes from the family heterorhabditidae. during growth in the insect, photorhabdus bacteria produce a broad-spectrum antibiotic identified as 3,5-dihydroxy-4-isopropylstilbene (st). the biochemical pathway responsible for the pro ...200516079333
pleiotropic role of quorum-sensing autoinducer 2 in photorhabdus luminescens.bacterial virulence is an integrative process that may involve quorum sensing. in this work, we compared by global expression profiling the wild-type entomopathogenic photorhabdus luminescens subsp. laumondii tt01 to a luxs-deficient mutant unable to synthesize the type 2 quorum-sensing inducer ai-2. ai-2 was shown to regulate more than 300 targets involved in most compartments and metabolic pathways of the cell. ai-2 is located high in the hierarchy, as it controls the expression of several tra ...200617021191
cloning and heterologous expression of insecticidal-protein-encoding genes from photorhabdus luminescens tt01 in enterobacter cloacae for termite control.enterobacter cloacae, one of the indigenous gut bacteria of the formosan subterranean termite (coptotermes formosanus), was genetically modified with a transposon tn5 vector containing genes (tcda1 and tcdb1) encoding orally insecticidal proteins from the entomopathogenic bacterium photorhabdus luminescens subsp. laumondii tt01, a symbiont of the entomopathogenic nematode heterorhabditis bacteriophora, for termite control. in the laboratory, termites were fed filter paper inoculated with the rec ...200818836027
identification and characterization of a novel l-amino acid ligase from photorhabdus luminescens subsp. laumondii tt01.l-amino acid ligase catalyzes dipeptide synthesis from unprotected l-amino acids in an atp-dependent manner. we recently identified a new member of l-amino acid ligase, the plu1440 protein, from photorhabdus luminescens subsp. laumondii tt01 by in silico analysis. this protein was found to synthesize dipeptides containing l-asparagine at the n-terminus, which is a novel substrate specificity.201020541113
genomic analysis reveals versatile organisms for quorum quenching enzymes: acyl-homoserine lactone-acylase and -lactonase.microbial virulence and their resistance to multiple drugs have obliged researchers to look for novel drug targets. virulence of pathogenic microbes is regulated by signal molecules such as acylated homoserine lactone (ahl) produced during a cell density dependent phenomenon of quorum sensing (qs). in contrast, certain microbes produce ahl-lactonases and -acylases to degrade qs signals, also termed as quorum quenching. mining sequenced genome databases has revealed organisms possessing conserved ...201121660112
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