| purification, molecular cloning, and application of a novel sphingomyelin-binding protein (clamlysin) from the brackishwater clam, corbicula japonica. | a novel sphingomyelin-binding protein (clamlysin) was purified from the foot muscle of a brackishwater clam, corbicula japonica. the purified 24.8-kda protein lysed sheep, horse and rabbit erythrocytes and the hemolytic activity was inhibited by sphingomyelin, but not other phospholipids or glycosphingolipids. the open reading frame of the clamlysin gene encoded a putative 26.9-kda protein (clamlysin b) which showed high sequence similarity with the actinoporin family. a surface plasmon resonanc ... | 2011 | 21396484 |
| detection of hepatitis e virus rna from the bivalve yamato-shijimi (corbicula japonica) in japan. | to evaluate whether bivalves are contaminated with hepatitis e virus (hev), samples of a bivalve called yamato-shijimi (corbicula japonica) were examined for hev by reverse transcription-polymerase chain reaction. genotype 3 hev was detected from 2 of 32 packages of yamato-shijimi obtained from japanese rivers, which indicated that hev contaminates river water in japan. | 2007 | 17255247 |
| brackish water benthic shellfish (corbicula japonica) as a biological indicator for cryptosporidium parvum oocysts in river water. | | 2004 | 15058651 |
| structure of new carotenoids from corbicula clam corbicula japonica. | six new carotenoids, named corbiculaxanthin (1), corbiculaxanthin 3'-acetate (2), 6-epiheteroxanthin (3), 7',8'-didehydrodeepoxyneoxanthin (4), cyclopyrrhoxanthin (5), and hydratoperidinin (6), were isolated from the corbicula clam (shijimi in japanese), corbicula japonica. their structures were determined by chemical and spectral data. | 2005 | 16180810 |
| detection system of cryptosporidium parvum oocysts by brackish water benthic shellfish (corbicula japonica) as a biological indicator in river water. | the brackish water benthic shellfish, corbicula japonica, was experimentally exposed to cryptosporidium parvum oocysts at 1.51x10(4)oocysts/clam/day for 7 or 14 days. oocysts were predominantly eliminated through the feces of corbicula japonica in both cases by microscopic and pcr methods. the fecal excretion rates of oocysts within 4 days after the last exposure to corbicula japonica were 87.6% for the 7-day exposure group and 86.0% for the 14-day exposure group. the tissue residue level of ooc ... | 2006 | 16998637 |
| molecular cloning of glycoside hydrolase family 45 cellulase genes from brackish water clam corbicula japonica. | we previously reported endogenous glycoside hydrolase family (ghf) 9 beta-1,4-glucanase gene, cjcel9a, from common japanese freshwater clam corbicula japonica. here we identified another endogenous beta-1,4-glucanase genes which belong to ghf45 (cjcel45a, cjcel45b). both genes encode orf of 627 bp corresponding to 208 amino acids. cjcel45a and cjcel45b are different in 5' and 3'-untranslated regions and six nucleotides in the orf. cjcel45 has only one ghf45 catalytic domain without any carbohydr ... | 2009 | 19266619 |
| influence of low-temperature processing of the brackish-water bivalve, corbicula japonica, on the ornithine content of its extract. | the ornithine content of an extract of the brackish-water bivalve, corbicula japonica, increased when the bivalve was frozen. it was not influenced by the period of freezing. this phenomenon was not apparent in the scallop, little-neck clam, or hard clam. we applied various low-temperature conditions for processing the bivalve from 4 degrees c to -10 degrees c and measured the ornithine content of each extract. the ornithine content was maximized by processing at - 4 degrees c. the increase in t ... | 2004 | 15215585 |
| two corbicula (corbiculidae: bivalvia) mitochondrial lineages are widely distributed in asian freshwater environment. | we investigated the biogeography of asian corbicula using mitochondrial gene sequence variation for corbicula members sampled from 24 localities of eight asian regions. a total of 210 individuals were genetically characterized by examining sequence variations of a 614 bp fragment of the mitochondrial cytochrome oxidase i (coi) gene. phylogenetic analyses of the coi dataset revealed that corbicula members are subdivided into two well-supported clades: estuarine and freshwater. a robust dichotomy ... | 2003 | 14615191 |
| purification and properties of a proteinaceous toxin from the brackishwater clam (corbicula japonica). | water extracts from the brackishwater clam (corbicula japonica) are lethal to mice upon i.v. injection. further mouse assays confirmed that the toxicity exhibits a regional variation but no seasonal or sexual variations. the c. japonica toxin was purified from foot muscle, the most toxic tissue, successively by hydrophobic chromatography on phenyl sepharose, gel filtration on sephacryl s-200, hydrophobic fplc on phenyl superose and cation-exchange fplc on mono s. the purified toxin had an i.v. l ... | 2001 | 11223096 |
| luminol-dependent chemiluminescence of hemocytes derived from marine and estuarine molluscs. | hemocytes of two marine molluscs, nerita albicilla (gastropod) and mytilus edulis (bivalve), were stimulated in vitro with zymosan and live cells of vibrio parahaemolyticus and escherichia coli as determined by luminol-dependent chemiluminescence (cl). the cl response was enhanced in the presence of the respective molluscan plasma. hemocytes of an estuarine gastropod, clithon retropictus, showed low cl response to zymosan and v. parahaemolyticus, which was slightly enhanced in the presence of c. ... | 1993 | 8513011 |
| reduction of lipogenic enzymes by shellfish triglycerides in rat liver. | when rats were fed for 2 weeks on 3% fat diets containing 0.5 or 1%corbicula (corbicula japonica prime), clam (tapes japonica) or oyster (callocorchina) triglycerides, serum and liver triglyceride levels were significantly lowered. the activities of hepatic glucose-6-phosphate dehydrogenase, malic enzyme and acetyl-coa carboxylase were markedly reduced in the rats. cholesterol synthesis by liver slices was also reduced. the results of immunochemical titrations and ouchterlony double-diffusion an ... | 1980 | 6105173 |
| chemotactic activity of hemocytes derived from a brackish-water clam, corbicula japonica, to vibrio parahaemolyticus and escherichia coli strains. | hemocytes from adult and juvenile specimens of a brackish-water clam, corbicula japonica, were attracted chemotactically to live cells of vibrio parahaemolyticus and escherichia coli strains in a balanced salt solution, which was enhanced significantly in the presence of respective c. japonica plasma. chemotactic attractions of adult's and juvenile's hemocytes were seen also in artificial seawater at a similar level to those in the balanced salt solution. chemotactic attractions of juvenile's he ... | 1992 | 1420564 |
| survival of kanagawa-positive strains of vibrio parahaemolyticus in a brackish-water area. | vibrio parahaemolyticus was observed to overwinter in sediments and to be present in considerable numbers in sediments and clithon retropictus (gastropod mollusc) during summer months at a brackish-water area along hashizu creek in japan. the highest level of the organisms was 9.3 x 10(6) and 2.3 x 10(7)/100 g in sediments and c. retropictus respectively. production of kanagawa haemolysin was detected in approximately 12% and 20% of strains isolated from sediments and c. retropictus respectively ... | 1985 | 4067291 |
| bioaccumulation and metabolism of [14c]bisphenol a in the brackish water bivalve corbicula japonica. | the brackish water bivalve mollusk corbicula japonica was exposed to brackish water containing approximately 9 microg/l [(14)c]bisphenol a (bpa) for 168 h (the uptake phase), and subsequently transferred to clean brackish water for 144 h (the depuration phase) under semi-static conditions. mono and disulfate conjugates of bpa were detected in the bivalves as major metabolites. at the end of the uptake phase, the visceral mass contained the highest (14)c-concentration, and the monosulfate conjuga ... | 2008 | 19060404 |
| molecular cloning of endogenous beta-glucosidase from common japanese brackish water clam corbicula japonica. | studies on the cellulose utilization by animals have been conducted in keeping with the recent developments in molecular biology. in mollusks, endogenous cellulases have been reported from blue mussel, abalone, and freshwater snail. we previously reported the possibility of cellulose assimilation by corbicula japonica, a representative bivalve dominant in brackish water environments in japan, and the cloning of its endogenous cellulase (beta-1,4-glucanase) gene (sakamoto, k., touhata, k., yamash ... | 2009 | 19393177 |
| putative endogenous xylanase from brackish-water clam corbicula japonica. | xylan digestion by corbicula japonica was investigated according to the hypothesis that c. japonica can breakdown xylan as well as cellulose. c. japonica showed relatively high xylanase activity compared to other bivalve species. molecular cloning of a xylanase gene was performed in order to determine whether c. japonica possesses an endogenous xylanase, and resulted in the isolation of cdna with an orf of 2523 bp corresponding to 840 amino acids (cjxyn10a). cjxyn10a has a glycoside hydrolase fa ... | 2009 | 19454320 |
| a comparative study of cellulase and hemicellulase activities of brackish water clam corbicula japonica with those of other marine veneroida bivalves. | corbicula japonica is a typical brackish water bivalve species belonging to the order veneroida, and it is the most important inland fishery resource in japan. corbicula japonica has been suggested to assimilate organic matter from terrestrial plants, unlike ruditapes philippinarum and mactra veneriformis, which selectively assimilate organic matter of marine origin. this led us to hypothesize that c. japonica, despite being a suspension feeder, could assimilate cellulosic materials derived from ... | 2009 | 19684215 |
| bioconcentration and biotransformation of [¹⁴c]methoxychlor in the brackish water bivalve corbicula japonica. | to obtain basic information on the metabolic fate of xenobiotics in the brackish water, bivalve corbicula japonica, bioconcentration and biotransformation experiments were performed using methoxychlor (mxc) as a model compound. bivalves were exposed to [ring-u-¹⁴c]mxc (10 µg l⁻¹) for 28 days under semi-static conditions followed by a 14-day depuration phase. the ¹⁴c concentration in the bivalves rapidly increased and reached a steady state after exposure for 7 days (bcfss = 2010); however, it ra ... | 2011 | 21521078 |
| complete genome sequence of oscillibacter valericigenes sjm18-20(t) (=nbrc 101213(t)). | oscillibacter valericigenes is a mesophilic, strictly anaerobic bacterium belonging to the clostridial cluster iv. strain sjm18-20(t) (=nbrc 101213(t) =dsm 18026(t)) is the type strain of the species and represents the genus oscillibacter iino et al. 2007. it was isolated from the alimentary canal of a japanese corbicula clam (corbicula japonica) collected on a seacoast in shimane prefecture in japan. phylogenetically, strain sjm18-20(t) is closest to uncultured bacteria in digestive tracts, inc ... | 2012 | 23408234 |
| in vitro acylation of okadaic acid in the presence of various bivalves' extracts. | the dinoflagellate dinophysis spp. is responsible for diarrhetic shellfish poisoning (dsp). in the bivalves exposed to the toxic bloom of the dinoflagellate, dinophysistoxin 3 (dtx3), the 7-oh acylated form of either okadaic acid (oa) or dtx1, is produced. we demonstrated in vitro acylation of oa with palmitoyl coa in the presence of protein extract from the digestive gland, but not other tissues of the bivalve mizuhopecten yessoensis. the yield of 7-o-palmitoyl oa reached its maximum within 2 h ... | 2013 | 23434830 |
| phylogeography of the brackish water clam corbicula japonica around the japanese archipelago inferred from mitochondrial coii gene sequences. | we investigated the phylogeography of the asian brackish water clam, corbicula japonica, to clarify its demographic history using partial mitochondrial coii gene sequences (990 bp) from 283 individuals collected from around the japanese archipelago and adjacent areas. phylogenetic analyses revealed the presence of two major groups within our samples: monophyletic group i comprising lineages a-e of c. japonica and paraphyletic group ii consisting of corbicula sp. lineages a-c were distributed in ... | 2014 | 24601779 |
| dynamic changes in the accumulation of metabolites in brackish water clam corbicula japonica associated with alternation of salinity. | the brackish water clam corbicula japonica inhabits rivers and brackish waters throughout japan where the major fishing grounds in the ibaraki prefecture, japan, are located at the hinuma lake and hinuma river. water salinity in the lake hinuma is low and stable due to the long distance from the pacific ocean, whereas that in the downstream of the river varies daily due to a strong effect of tidal waters. in the present study, we dissected the gill and foot muscle of brackish water clam collecte ... | 2015 | 25455471 |
| immunohistochemical, in situ hybridization and biochemical studies on endogenous cellulase of corbicula japonica. | we previously reported on the endogenous cellulase gene of corbicula japonica, cjcel9a. in this study, the tissue localization of the mrna and translated products of cjcel9a was investigated in order to understand how this gene is physiologically involved in cellulose decomposition by c. japonica. antiserum against recombinant cjcel9a protein was prepared. multiple bands were observed mainly on western blot analysis of the crystalline style, and the band sizes partially corresponded to the activ ... | 2008 | 18436461 |
| a novel ornithine-containing tripeptide isolated from the extract of the brackish-water bivalve corbicula japonica. | previous studies have demonstrated that frozen preparations of the brackish-water bivalve corbicula japonica significantly increase the content of free ornithine found in its extracts. here we report a novel ornithine-containing tripeptide commonly found in c. japonica, which is believed to be the source of increased free ornithine. the new peptide, named acorbine, was isolated from extracts of this bivalve obtained using ultra-filtration and gel permeation chromatography. acorbine is comprised ... | 2007 | 17293050 |
| purification and molecular cloning of a dna adp-ribosylating protein, carp-1, from the edible clam meretrix lamarckii. | the cabbage butterflies pieris rapae and pieris brassicae have unique enzymes, named pierisin-1 and -2, respectively, that catalyze the adp-ribosylation of guanine residues of dna, which has been linked with induction of apoptosis and mutation in mammalian cell lines. in the present study, we identified adp-ribosylation activity targeting dna in six kinds of edible clam. similar to our observations with pierisin-1 and -2, crude extracts from the clams meretrix lamarckii, ruditapes philippinarum, ... | 2006 | 16945908 |
| [seasonal characteristics of gametogenesis in the corbicula japonica]. | a morphological study of gonads in corbicula japonica made it possible to distinguish between five stages of gonad maturity. it has been established that stages of active gametogenesis, prespawning and of spawning go almost in parallel and in the shortest time. in july, with the beginning of spawning, gametogenesis does not stop, and oogenesis proceeds up to the end of august. dynamics of changes of some chemical substances in oocytes was followed. high concentrations of, respectively, rna, albu ... | 2006 | 16737182 |
| carotenoids in three species of corbicula clams, corbicula japonica, corbicula sandai, and corbicula sp. (chinese freshwater corbicula clam). | carotenoids were investigated in three species of corbicula clams, corbicula japonica, corbicula sandai, and corbicula sp. (chinese freshwater corbicula clam). forty-three carotenoids were isolated. among them, 7,8-didehydro-beta-cryptoxanthin (12), peridininol 5,8-furanoxide (38), pyrrhoxanthin 5,8-furanoxide (40), and pyrrhoxanthinol 5,8-furanoxide (43) are newly reported as naturally occurring carotenoids. their structures were characterized on the basis of uv-vis, fab-ms including ms/ms expe ... | 2005 | 16218688 |
| two-domain arginine kinases from the clams solen strictus and corbicula japonica: exceptional amino acid replacement of the functionally important d(62) by g. | arginine kinases (aks) isolated from the adductor muscle of the clams solen strictus and corbicula japonica have relative molecular masses of 80 kda as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) in contrast to the 40 kda aks found in mollusca and arthropoda. the cdnas encoding solen and corbicula aks have open reading frames of 2175 nucleotides (724 amino acid protein) and 2172 nucleotides (723 amino acid protein), respectively. the amino acid sequence clea ... | 2002 | 12127572 |
| glycolipid transfer protein from pig brain transfers glycolipids with beta-linked sugars but not with alpha-linked sugars at the sugar-lipid linkage. | the glycolipid transfer protein purified from pig brain facilitates the transfer of various glycosphingolipids and glyceroglycolipids (yamada, k., abe, a. and sasaki, t. (1985) j. biol. chem. 260, 4615-4621). in this paper, the transfer of man beta 1----4glc beta 1-cer and man alpha 1----4man beta 1-cer isolated from a bivalve, corbicula japonica, the transfer of 3-[glc alpha 1-]-sn-1,2-diacylglycerol and 3-[glc alpha 1----2glc alpha 1-]-sn-1,2-diacylglycerol prepared from streptococcus lactis, ... | 1986 | 3778924 |
| purification and some properties of alanine racemase from a bivalve mollusc corbicula japonica. | a brackish-water mollusc, corbicula japonica, uses large quantities of d- and l-alanine as intracellular osmotically active solutes, osmolytes, for regulation of intracellular osmolarity. we purified alanine racemase from the mantle of c. japonica to characterize its enzymological properties. the molecular masses of the enzyme were estimated to be 41 kda by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 140 kda by gel filtration on high-performance liquid chromatography, suggestin ... | 2001 | 11169488 |
| detection of a proteinaceous toxin in the brackishwater clam (corbicula japonica). | water extracts from the brackishwater clam (corbicula japonica) were found to be lethal to mice upon i.v. injection. muscular tissues (foot muscle, adductor muscle, mantle muscle, mantle and siphon) were all toxic while gill and viscera (mid-gut gland, testis and ovary) were nontoxic; the highest toxicity was observed with foot muscle. the toxin was judged to be a thermolabile, basic protein with a mol. wt. of about 13,000. | 1995 | 7660374 |
| ecological cycle of thermostable direct hemolysin-producing strains of vibrio parahaemolyticus in a brackish-water area with special reference to molluscs and attached microalgae. | prevalences of thermostable direct hemolysin (tdh)-producing strains in communities of a gastropod mollusc. clithon retropictus, and a bivalve mollusc, corbicula japonica, and levels of the strains in attached microalgae and muddy sediments were investigated at a brackish-water area along hashizu creek and togo pond in japan, v. parahaemolyticus was detected from attached microalgae at hashizu creek in summer months with the highest level of 1.4 x 10(5) cfu/g. levels of the organism among 20 ani ... | 1991 | 1830798 |
| effect of feeding the shell fish (corbicula japonica) on lipid metabolism in the rat. | rats were maintained for 2 weeks on 3 different diets; a basal diet, one containing 0.1% cholate, and one containing 0.1% cholesterol and 0.1% cholate. each dietary group was further divided into subgroups whose diet contained 0, 5 or 10% (dry weight) of minced corbicula (corbicula japonica prime). feeding corbicula significantly reduced the increase of cholesterol levels in rats fed the cholesterol diet. though corbicula contains several sterols, sterols other than cholesterol were almost not a ... | 1979 | 497041 |
| kinetic properties and structural characteristics of an unusual two-domain arginine kinase of the clam corbicula japonica. | arginine kinase (ak) from the clam corbicula japonica is a unique enzyme in that it has an unusual two-domain structure with molecular mass of 80 kda. it lacks two functionally important amino acid residues, asp-62 and arg-193, which are conserved in other 40 kda aks and are assumed to be key residues for stabilizing the substrate-bound structure. k m arg and vmax values for the recombinant two-domain ak were determined. these values were close to those of usual 40 kda aks, although corbicula ak ... | 2003 | 12505165 |
| preliminary analyses on persistence of vibrio parahaemolyticus in a brackish water clam, corbicula japonica. | | 1986 | 3712889 |