| isolation and characterization of a unique group of slowly growing mycobacteria: description of mycobacterium lentiflavum sp. nov. | a distinct group of slowly growing mycobacteria was identified on the basis of growth characteristics, biochemical and lipid profiles, and nucleic acid analyses. the isolates showed growth at 22 to 37 degrees c, yellow pigmentation, and negative tests for tween 80 hydrolysis, nicotinic acid, nitrate reductase, and urease; tests for arylsulfatase, pyrazinamidase, and heat-stable catalase were variable. analysis of cellular fatty acids by gas-liquid chromatography and mycolic acids by thin-layer c ... | 1996 | 8727884 |
| characterization of mycobacterial isolates phylogenetically related to, but different from mycobacterium simiae. | the use of high-performance liquid chromatography (hplc) revealed four previously unreported profiles within a group of mycobacteria consisting of 14 clinical isolates. these mycobacteria, whose identification by conventional tests appeared problematic, mostly resembled mycobacterium avium complex or mycobacterium simiae. genetic analysis revealed, within this group, six different nucleic acid sequences in a hypervariable 16s rrna segment, but all the isolates appeared to be phylogenetically rel ... | 1997 | 9041415 |
| mycobacterium lentiflavum: an etiologic agent of cervical lymphadenitis. | | 1997 | 9402392 |
| identification and characterization of is2404 and is2606: two distinct repeated sequences for detection of mycobacterium ulcerans by pcr. | molecular analysis of mycobacterium ulcerans has revealed two new insertion sequences (iss), is2404 and is2606. is2404 was identified by complete sequencing of a previously described repetitive dna segment from m. ulcerans. this element is 1,274 bp long, contains 12-bp inverted repeats and a single open reading frame (orf) potentially encoding a protein of 327 amino acids (aa), and apparently generates 7-bp direct repeats upon transposition. amino acid similarity was found between the putative t ... | 1999 | 10074520 |
| the pcr amplification of non-tuberculous mycobacterial 16s rrna sequences from soil. | non-tuberculous mycobacteria are free living saprophytic organisms commonly found in soil and water. some are major causes of opportunistic infection, particularly in immuno-compromised patients, and may influence the efficacy of bacille calmette-guérin vaccinations. many of these organisms are not amenable to culture, so information about their distribution is limited. pcr primers designed to amplify part of the mycobacterial 16s rrna gene were applied to dna extracted from cultured organisms a ... | 2000 | 10754246 |
| molecular characterization of environmental mycobacterium strains by pcr-restriction fragment length polymorphism of hsp65 and by sequencing of hsp65, and of 16s and its1 rdna. | fifteen mycobacterial strains from the environment, not clearly identifiable by biochemical properties, were analyzed with molecular markers: pcr-restriction enzyme analysis of hsp65 and sequencing of hsp65, and of the internal transcribed spacer 1 (its1) and 16s rdna. the 16s rdna sequencing closely related the strains to a slow-growing mycobacterial group including mycobacterium simiae, mycobacterium lentiflavum, mycobacterium genavense, mycobacterium triplex and mycobacterium heidelbergense. ... | 2000 | 11081578 |
| disseminated mycobacterium lentiflavum infection in a human immunodeficiency virus-infected patient. | we report the first case of mycobacterium lentiflavum disseminated infection in a human immunodeficiency virus-infected patient. conventional identification procedures failed to identify the mycobacterial strain, but sequencing of the 16s rrna gene led to the species identification. furthermore, we describe here the analysis of the 16s-23s rrna internal transcribed spacer sequence of m. lentiflavum. | 2001 | 11326043 |
| human infections due to mycobacterium lentiflavum. | three cases of human disease due to mycobacterium lentiflavum are reported. in the first, the mycobacterium was responsible for chronic pulmonary disease in an elderly woman; in the second, it gave rise to cervical lymphadenitis in a child; and in the third, it caused a liver abscess in a young aids patient. | 2002 | 11826009 |
| [isolation of mycobacterium lentiflavum in a case of suspected lung cancer]. | | 2002 | 11886682 |
| cervical lymphadenitis caused by mycobacterium lentiflavum. | we report a case of human infection caused by mycobacterium lentiflavum and review the literature for infections caused by this bacterium. the patient was a 19-month-old boy with involvement of a cervical lymph node. surgical removal of the lymphadenopathy was both diagnostic and curative. | 2002 | 12182389 |
| clinical and epidemiological correlates of genotypes within the mycobacterium avium complex defined by restriction and sequence analysis of hsp65. | species identification of isolates of the mycobacterium avium complex (mac) remains a difficult task. although m. avium and mycobacterium intracellulare can be identified with expensive, commercially available probes, many mac isolates remain unresolved, including those representing mycobacterium lentiflavum as well as other potentially undefined species. pcr restriction analysis (pra) of the hsp65 gene has been proposed as a rapid and inexpensive approach. we applied pra to 278 mac isolates, in ... | 2002 | 12202581 |
| disseminated infection caused by slow-growing mycobacterium lentiflavum. | | 2002 | 12373506 |
| gas-chromatographic lipid profiles in identification of currently known slowly growing environmental mycobacteria. | cellular fatty acid analysis by glc is widely used in the species identification of mycobacteria. combining mycolic acid cleavage products with shorter cellular fatty acids increases the informative value of the analysis. a key has been created to aid in the identification of all currently known slowly growing environmental species. in this scheme, the species are classified into six categories, each characterized by a combination of fatty markers shared by those species. within each category, i ... | 2003 | 12676870 |
| [mycobacterium lentiflavum infection: case history and review of the medical literature]. | | 2003 | 12732120 |
| [clinically insignificant mycobacterium lentiflavum isolation in 30 patients]. | | 2004 | 14987544 |
| mycobacteria in water and loose deposits of drinking water distribution systems in finland. | drinking water distribution systems were analyzed for viable counts of mycobacteria by sampling water from waterworks and in different parts of the systems. in addition, loose deposits collected during mechanical cleaning of the main pipelines were similarly analyzed. the study covered 16 systems at eight localities in finland. in an experimental study, mycobacterial colonization of biofilms on polyvinyl chloride tubes in a system was studied. the isolation frequency of mycobacteria increased fr ... | 2004 | 15066787 |
| mycobacterium parmense sp. nov. | the isolation and identification of a novel, slow-growing, scotochromogenic, mycobacterial species is reported. a strain, designated mup 1182t, was isolated from a cervical lymph node of a 3-year-old child. mup 1182t is alcohol- and acid-fast, with a lipid pattern that is consistent with those of species that belong to the genus mycobacterium. it grows slowly at 25-37 degrees c, but does not grow at 42 degrees c. the isolate was revealed to be biochemically distinct from previously described myc ... | 2004 | 15280280 |
| mycobacterium lentiflavum as an emerging causative agent of cervical lymphadenitis. | a lymph node excision was performed on a 45-year-old woman with left cervical swelling. the disorder which developed after the patient had undergone oral surgery for a severe periodontal disease failed to respond to antimicrobial chemotherapy. a mycobacterial strain subsequently identified by high-performance liquid chromatography analysis of cell wall mycolic acids as mycobacterium lentiflavum grew from the excised specimen. this case and previously published reports highlight the relevance of ... | 2004 | 15297560 |
| mycobacterium lentiflavum infection in immunocompetent patient. | mycobacterium lentiflavum is a recently described nontuberculous mycobacterium that has mainly clinical importance in young children with cervical lymphadenitis and in immunocompromised patients. we describe a case of chronic pulmonary infection in an immunocompetent patient. our observation confirms clinical, diagnostic, and treatment difficulties in the management of m. lentiflavum infection. | 2005 | 15705334 |
| comparison of naoh-n-acetyl cysteine and sulfuric acid decontamination methods for recovery of mycobacteria from clinical specimens. | we compared the naoh-n-acetyl cysteine (naoh-nalc) and the sulfuric acid decontamination procedure in the detection of mycobacteria using the mycobacteria growth indicator tube (mgit). in total 219 sputum specimens were collected from 142 zambian patients and subjected to mycobacterial culture. one half of the specimen was decontaminated with naoh-nalc and the other half was decontaminated with sulfuric acid. from the 438 samples a total of 261 (60%) cultures yielded growth of mycobacteria, cons ... | 2005 | 15823396 |
| mycobacterium florentinum sp. nov., isolated from humans. | eight mycobacterial strains isolated during an 11 year period from the sputum of independent patients with various pulmonary disorders and, in one case, from a lymph node of a young girl, were found to present identical features. phenotypic and genotypic characteristics revealed that the most closely related species to these test isolates were mycobacterium triplex and mycobacterium lentiflavum. however, the lipids of the cell wall of the test isolates differed from those of the latter species b ... | 2005 | 15879240 |
| mycobacterium lentiflavum, a recently identified slow-growing mycobacterial species: clinical significance in immunosuppressed cancer patients and summary of reported cases of infection. | the clinical significance of mycobacterium lentiflavum, a recently identified nontuberculous mycobacterium, remains uncertain, especially in immunosuppressed cancer patients. the records of all patients in whom m. lentiflavum was identified using a gene sequencing technique between january 2001 and december 2003 were reviewed. the mean age among 12 patients was 51+/-20 years, and 11 (92%) patients had a hematologic malignancy. six of seven (86%) hematopoietic stem cell transplant recipients had ... | 2005 | 16133412 |
| mycobacterium lentiflavum, an emerging pathogen? | three cases of infection due to mycobacterium lentiflavum, a recently described species characterized by multiple resistance to anti-mycobacterial drugs, are reported here. while one case simply adds to the number of cervical lynphadenitis reported in literature, the others concern the first isolations from pleural effusions, in a young boy with leukaemia and in an elderly patient with lung disease, respectively. | 2006 | 16223526 |
| isolation of nontuberculous mycobacteria in zambia: eight case reports. | the isolation of nontuberculous mycobacteria (ntm) raises the question of their clinical significance, especially in an african setting. we found a high percentage of ntm isolated from various specimens, including ones that are normally sterile, among 213 patients in zambia. because tuberculosis can affect all parts of the body, we decided to include patients who had signs and symptoms in any part of the body for more than 2 weeks. most patients had tractus respiratorius (80%) and tractus digest ... | 2005 | 16333092 |
| detection of mixed infections with mycobacterium lentiflavum and mycobacterium avium by molecular genotyping methods. | three mycobacterial isolates, one from the blood of an hiv-infected patient and two consecutive isolates from a woman with unknown hiv status, had been identified as belonging to the mycobacterium avium complex by conventional procedures. in both patients, using genetic analysis procedures such as pcr-restriction enzyme analysis (pra) of the hsp65 gene, a commercially available reverse hybridization-based assay (inno-lipa mycobacteria) and/or sequencing analysis of the 16s-23s internal transcrib ... | 2006 | 16388042 |
| isolation and automated ribotyping of mycobacterium lentiflavum from drinking water distribution system and clinical specimens. | automated ribotyping as a tool for identifying of nontuberculous mycobacteria was evaluated. we created a database comprising of riboprints of 60 strains, representing 32 species of nontuberculous mycobacteria. it was shown that combined ribopatterns generated after digestion with ecori and pvuii were distinguishable between species of both slow-growing and rapid-growing mycobacteria. the findings were in good agreement with the 16s rrna gene sequencing results, allowing correct identification o ... | 2006 | 16499612 |
| cutaneous infection by mycobacterium lentiflavum in a patient with hiv. | | 2006 | 16902301 |
| [mycobacterium lentiflavum lymphadenitis]. | | 2007 | 17583659 |
| mycobacterial species diversity at a general hospital on the island of crete: first detection of mycobacterium lentiflavum in greece. | the objective of the present study was to investigate the diversity of mycobacterial isolates in a general hospital in crete, greece. 48 positive lowenstein-jensen cultures over a 3-y period were analysed by means of accuprobe and genotype assays. non-tuberculous mycobacteria (ntm) comprised the majority of the isolates (56.3%, 27/48) vs 33.3% (16/48) of m. tuberculosis; 10.4% of the isolates could not be classified. among ntm, m. lentiflavum was the predominant species isolated (9/27) followed ... | 2007 | 17852893 |
| isolation of mycobacterium lentiflavum from a patient with a lung destroyed by tuberculosis. | mycobacterium lentiflavum has recently been described as an emerging human pathogen without regard to the immune status of the host. we herein report on m. lentiflavum isolated from a respiratory specimen of a patient. although the organism described in this case seems to be a colonizer of a lung destroyed by tuberculosis, the current methods for species identification of nontuberculous mycobacteria have to be re-evaluated so as not to underestimate these organisms. | 2007 | 18094563 |
| [intraspecies divergence of 16s rdna, its, rpob gene and hsp65 gene sequence for mycobacterium lentiflavum]. | to clarify the genetic microheterogeneity of mycobacterium lentiflavum and identify the predominant genotype. | 2008 | 18536332 |
| a novel approach to correct variations in raman spectra due to photo-bleachable cellular components. | bacterial typing by raman spectroscopy is based on small spectral differences that exist between strains, due to differences in their overall molecular composition. these strain-specific spectral differences can be obscured by sources of non-specific signal variance. one such source is the signal contribution of microbial pigments that can vary strongly in intensity. examples of such pigments are carotenoids in staphylococcus aureus, and other pigments in pseudomonas aeruginosa and mycobacterium ... | 2009 | 19173067 |
| identification of non-tuberculous mycobacteria: utility of the genotype mycobacterium cm/as assay compared with hplc and 16s rrna gene sequencing. | non-tuberculous mycobacteria (ntm) causing clinical disease have become increasingly common and more diverse. a new reverse line probe assay, genotype mycobacterium cm/as (hain lifescience), was evaluated for identification of a broad range of ntm. it was compared with phenotypic (hplc) and molecular (dna probes, in-house real-time multiplex species-specific pcr, 16s rrna gene pcr and sequencing) identification techniques, which together provided the reference 'gold standard'. a total of 131 cli ... | 2009 | 19502366 |
| detection of nontuberculous mycobacteria from water buffalo raw milk in brazil. | milk is an important nutritional source to man and water buffalo raw milk is used to produce mozzarella cheese. products from unpasteurized milk have been associated with certain infectious diseases and can carry pathogenic mycobacteria. nontuberculous mycobacteria (ntm) are emerging pathogens causing opportunistic infections in humans and animals. the objectives of this study were to demonstrate the presence of mycobacteria in water buffaloes' milk and to determine their role as possible source ... | 2009 | 19527843 |
| nontuberculous mycobacterial infection in children: a prospective national study. | the epidemiology and management of nontuberculous mycobacterial (ntm) infection in australian children is unknown. | 2009 | 19636280 |
| [erythema nodosum as a first sign of adenitis due to mycobacterium lentiflavum]. | | 2009 | 19733137 |
| isolation of non-tuberculous mycobacteria at three rural settings in zambia; a pilot study. | to assess the role of non-tuberculous mycobacteria (ntm) as a cause of tuberculosis-like diseases in zambia, 167 chronically ill patients, hospitalized in three rural hospitals in katete, sesheke and chilonga, were examined by microscopy and liquid culture for the presence of ntm. the percentages of patients with a positive culture for mycobacterium tuberculosis complex were similar in the three geographical locations (19-25%). in contrast, the percentage of ntm ranged from 78% in katete and 65% ... | 2010 | 19832715 |
| current treatment of atypical mycobacteriosis. | atypical mycobacteria are a heterogeneous group of organisms that are of increasing importance because of the growing number of infections they cause. this rising rate of infection is due mainly to the increase in the number of susceptible (and especially immunosuppressed) patients. | 2009 | 19929702 |
| inactivation of environmental mycobacteria by free chlorine and uv. | the resistance of environmental mycobacteria (em) against chlorine and ultraviolet (uv) was evaluated for determination of the ct value and uv dose to inactivate em. chlorine disinfection experiments were done on mycobacterium fortuitum in oxidant demand-free buffered water at the worst condition (ph 8.5, 4 degrees c) and normal condition (ph 7.0, 20 degrees c). the ct value for 3log inactivation of m. fortuitum was 600 times greater than that of escherichia coli. uv experiments were performed f ... | 2010 | 19945731 |
| utility of high-performance liquid chromatography analysis of mycolic acids and partial 16s rrna gene sequencing for routine identification of mycobacterium spp. in a national reference laboratory. | high-performance liquid chromatography analysis of mycolic acids and partial gene sequencing for the first 500-bp 5' end of the 16s rrna gene were used singularly and in combination to evaluate the final identification of species. examination of 200 cultures revealed 100 strains of slowly growing mycobacteria (sgm), 91 strains of rapidly growing mycobacteria (rgm), and 9 strains of other genera. sgm were discriminated in complexes with both methods for 56 strains, composed primarily of the mycob ... | 2010 | 20466195 |
| mycobacterium sherrisii sp. nov., a slow-growing non-chromogenic species. | 'mycobacterium sherrisii' is an undescribed species that appears to be emerging, in particular, among hiv-positive patients originating from africa. to describe 'm. sherrisii', to ensure that the species name is validly published and to define its phylogenetic position, we collected 11 of these strains reported in five previous studies, and subjected them to biochemical identification, cell-wall mycolic acid analysis and sequencing of multiple housekeeping genes. the bacteria formed smooth and g ... | 2010 | 20639227 |
| mycobacterium lentiflavum--a cause of infections in the head and neck: case report and literature review. | | 2011 | 20674119 |
| mycobacterium lentiflavum in drinking water supplies, australia. | mycobacterium lentiflavum, a slow-growing nontuberculous mycobacterium, is a rare cause of human disease. it has been isolated from environmental samples worldwide. to assess the clinical significance of m. lentiflavum isolates reported to the queensland tuberculosis control centre, australia, during 2001-2008, we explored the genotypic similarity and geographic relationship between isolates from humans and potable water in the brisbane metropolitan area. a total of 47 isolates from 36 patients ... | 2011 | 21392429 |
| mycobacterium lentiflavum ileitis using aspirated intestinal fluid during endoscopy in hiv-infected patient. | | 2011 | 21699576 |
| drug susceptibility testing and pharmacokinetics question current treatment regimens in mycobacterium simiae complex disease. | the mycobacterium simiae complex bacteria can cause opportunistic infections in humans. in the case of definite disease, there are no evidence-based treatment regimens and outcomes are very disappointing. to increase the evidence base underpinning treatment regimens for m. simiae complex disease, drug susceptibility patterns and rifampicin/ethambutol synergy were assessed retrospectively in 69 clinical m. simiae complex isolates from 60 patients (22 patients with m. simiae, 24 with mycobacterium ... | 2011 | 22099521 |
| isolation of multiple nontuberculous mycobacteria species in the same patients. | during the observation of many patients with nontuberculous mycobacteria (ntm) infections, we have often isolated different ntm species in the same patient. in this study we elucidated the patterns of multiple ntm species isolation. | 2011 | 21873093 |
| nontuberculous mycobacteria: an underestimated cause of bioprosthetic valve infective endocarditis. | background. atypical mycobacteria, or nontuberculous mycobacteria (ntm), have been barely reported as infective endocarditis (ie) agents. methods. from january 2010 to december 2013, cardiac valve samples sent to our laboratory as cases of blood culture-negative suspected ie were analyzed by 16s rdna polymerase chain reaction (pcr). when positive for ntm, hsp pcr allowed species identification. demographic, clinical, echocardiographic, histopathological, and ziehl-neelsen staining data were th ... | 2015 | 26213691 |
| microbial pathogens in source and treated waters from drinking water treatment plants in the united states and implications for human health. | an occurrence survey was conducted on selected pathogens in source and treated drinking water collected from 25 drinking water treatment plants (dwtps) in the united states. water samples were analyzed for the protozoa giardia and cryptosporidium (epa method 1623); the fungi aspergillus fumigatus, aspergillus niger and aspergillus terreus (quantitative pcr [qpcr]); and the bacteria legionella pneumophila (qpcr), mycobacterium avium, m. avium subspecies paratuberculosis, and mycobacterium intrace ... | 2016 | 27260619 |
| [identification of mycobacterium spp. isolates using matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (maldi-tof ms)]. | matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (maldi-tof ms) has recently been widely used in diagnostic microbiological laboratories. it is a cheap and rapid method for the identification of bacteria and micromycetes. apart from this purpose, it is also used for the detection of antibiotic resistance mechanisms. it has the potential to be extended for other purposes in microbiology. the aim of this study was to validate maldi-tof ms for the identification of mycob ... | 2014 | 25412483 |
| prevalence and drug resistance of mycobacteria in turkish cystic fibrosis patients. | isolation of mycobacteria in cystic fibrosis (cf) patients is increasingly being reported. because of having long term antimicrobial treatment, cf patients are at risk of pulmonary infection with especially resistant nontuberculous mycobacteria (ntm) strains. the aim of the present study is to determine the prevalence of mycobacterium spp. and antimicrobial susceptibility in turkish cf patients. | 2014 | 25123237 |
| inverse correlation between salt tolerance and host-adaptation in mycobacteria. | the genus mycobacterium includes host-adapted organisms regarded as obligate and opportunistic pathogens and environmental organisms. factors contributing to this wide range of adaptations are poorly known. | 2016 | 27129386 |
| [lymphadenitis due to non-tuberculous mycobacteria: experience over 15 years]. | to study the epidemiology, clinical features, diagnosis, therapeutic management, and outcome of non-tuberculous mycobacterial lymphadenitis in a paediatric population of aragón (spain). | 2017 | 27052399 |
| [genetic analysis reveals misidentification of mycobacterium lentiflavum as mycobacterium intracellulare by the cobas taqman mai test]. | to evaluate cobas taqman mai test misidentification of mycobacterium lentiflavum as mycobacterium intracellulare. | 2014 | 25283011 |
| comparison of mycobacterium lentiflavum and mycobacterium avium-intracellulare complex lymphadenitis. | mycobacterium lentiflavum is considered a rare pathogen causing nontuberculous mycobacterial (ntm) lymphadenitis. | 2014 | 24064561 |
| [a case of pulmonary mycobacterium lentiflavum infection diagnosed by microbiological analysis]. | in 1998, a 51-year-old woman was diagnosed with mycobacterium avium infection on the basis of chest radiographic findings, positive smear test results, and positive results of polymerase chain reaction (pcr) specific for mycobacterium avium dna in bronchial lavage fluid. antimycobacterial therapy was administered for 11 months, and the chest radiographic findings improved. in 2001, re-treatment was performed because radiographic findings indicated exacerbation of disease and poor response. after ... | 2012 | 23367830 |
| isolation of nontuberculous mycobacteria from hospital waters in turkey. | nontuberculous mycobacteria (ntm) are ubiquitous in hot and cold water distribution systems. with molecular typing methods it was shown that water can be the source of colonization and infection with ntm. the aim of our study was the investigation of ntm in hot and cold water samples taken from various departments of two hospitals in istanbul, turkey. totally, 160 water samples were examined. the temperature, ph, and free chlorine levels of water samples were measured between 10-41 °c, 6.78-7.98 ... | 2013 | 23509870 |
| [an investigation of misidentification of mycobacterium lentiflavum as mycobacterium intracellulare by the cobas taqman mai test]. | | 2013 | 23713333 |
| [mycobacterium lentiflavum lymphadenitis: two case reports]. | lymphadenitis is the most common clinical feature in nontuberculous mycobacterium infection in immunocompetent children. we present two case reports of m. lentiflavum lymphadenitis diagnosed in a tertiary hospital in the last 10 years. routine tests were performed after persistent adenopathy, and a sample for culture was obtained, being positive for this microorganism. both patients received oral antibiotics during several weeks. case 1 needed complete excision after five months of treatment, wh ... | 2016 | 27606656 |
| prevalence of mycobacterium lentiflavum in cystic fibrosis patients, france. | mycobacterium lentiflavum is rarely isolated in respiratory tract samples from cystic fibrosis patients. we herein describe an unusually high prevalence of m. lentiflavum in such patients. | 2015 | 26503635 |
| acute necrotizing pneumonia combined with parapneumonic effusion caused by mycobacterium lentiflavum: a case report. | mycobacterium lentiflavum (m. lentiflavum), a slow growing nontuberculous mycobacterium (ntm), has recently been described as an emerging human pathogen regardless of the immune status of the host. previous reports have demonstrated that cervical lymphadenitis of children is the most frequent pathology of m. lentiflavum. however, there are little reports regarding pulmonary diseases by m. lentiflavum specifically in immunocompetent patients. | 2015 | 26286334 |
| draft genome sequence of mycobacterium lentiflavum csur p1491. | we announce the draft genome sequence of mycobacterium lentiflavum strain csur p1491, a nontuberculous mycobacterium responsible for opportunistic potentially life-threatening infections in immunocompromised patients. the genome described here comprises a 6,818,507-bp chromosome exhibiting a 65.75% g+c content, 6,354 protein-coding genes, and 75 rna genes. | 2015 | 26205866 |
| immunodeficiency in a child with rapadilino syndrome: a case report and review of the literature. | rapadilino syndrome is a genetic disease characterized by a characteristic clinical tableau. it is caused by mutations in recql4 gene. immunodeficiency is not described as a classical feature of the disease. we present a 2-year-old girl with rapadilino syndrome with important lymphadenopathies and pneumonia due to disseminated mycobacterium lentiflavum infection. an immunological work-up showed several unexpected abnormalities. repeated blood samples showed severe lymphopenia. immunophenotyping ... | 2015 | 26064716 |
| acid-fast smear and histopathology results provide guidance for the appropriate use of broad-range polymerase chain reaction and sequencing for mycobacteria. | new molecular diagnostic tests are attractive because of the potential they hold for improving diagnostics in microbiology. the value of these tests, which is often assumed, should be investigated to determine the best use of these potentially powerful tools. | 2015 | 25574585 |
| disseminated mycobacterium lentiflavum responsible for hemophagocytic lymphohistocytosis in a man with a history of heart transplantation. | mycobacterium lentiflavum is a nontuberculous, slowly growing mycobacterium usually recognized as a contaminant. here, we report a case of disseminated m. lentiflavum infection responsible for hemophagocytic lymphohistocytosis in a heart-transplanted man. | 2014 | 24871221 |
| nontuberculous mycobacterial lung disease caused by mycobacterium lentiflavum in a patient with bronchiectasis. | we report a rare case of lung disease caused by mycobacterium lentiflavum in a previously healthy woman. a 54-year-old woman was referred to our hospital due to chronic cough and sputum. a computed tomography scan of the chest revealed bilateral bronchiectasis with bronchiolitis in the right middle lobe and the lingular division of the left upper lobe. nontuberculous mycobacteria were isolated twice from three expectorated sputum specimens. all isolates were identified as m. lentiflavum by multi ... | 2013 | 23678361 |
| isolation and identification of environmental mycobacteria in the waters of a hemodialysis center. | the use of poorly treated water during hemodialysis may lead to contamination with nontuberculous mycobacteria (ntm). this study aimed to isolate and identify ntm species in the water of a brazilian hemodialysis center. we collected 210 samples of water from the hydric system of the unit (post-osmosis system, hemodialysis rooms, reuse system, and hemodialysis equipment) and from the municipal supply network; we isolated the ntm by a classic microbiological technique and identified them by the pc ... | 2013 | 23463515 |
| human infections due to mycobacterium lentiflavum: first report in iran. | non-tuberculosis mycobacteria (ntm), as certain species of mycobacteria, can affect human in several ways. in the preceding years, the rate of ntm detection has risen in both immunocompromised and immunocompetent patients. on the other hand, several reports have debated the possibility of co-infection of both mycobcateriu tuberculosis (mtb) and ntm in individuals that puts the role of ntm in disease manifestations under question. moreover, it is now proven that some of the cases that are identif ... | 2010 | 22347547 |
| species-specific risk factors, treatment decisions, and clinical outcomes for laboratory isolates of less common nontuberculous mycobacteria in washington state. | nontuberculous mycobacteria (ntm) are a diverse group of environmental organisms that infrequently cause human disease. understanding of the epidemiologic and clinical characteristics associated with ntm disease is needed to refine diagnostic and treatment strategies, particularly among the less commonly isolated species. | 2017 | 28387523 |
| recovery of mycobacterium lentiflavum from bronchial lavage during follow-up of an extrapulmonary tuberculosis patient. | initially diagnosed with cervical lymphadenitis, a 15-year-old boy was started with category i anti-tuberculosis (tb) drugs. follow-up investigations led to isolation and identification of mycobacterium lentiflavum by multiple diagnostic and identification approaches. observation of this rare pathogen from human origin urges cautious diagnosis while attending tb cases. | 2017 | 28776534 |