| detection of eutypa lata and eutypella vitis in grapevine by nested multiplex polymerase chain reaction. | abstract two fungi were isolated from grapevines in michigan vineyards with eutypa dieback symptoms: eutypa lata and eutypella vitis. these fungi are difficult to distinguish morphologically but are genetically distinct as determined by sequencing of the internal transcribed spacer (its) regions. the its regions of 25 eutypa lata and 15 eutypella vitis isolates were sequenced. eutypa lata sequences were more variable than those of eutypella vitis. polymerase chain reaction (pcr) primers were des ... | 2007 | 18943605 |