| detection of brazilian hantavirus by reverse transcription polymerase chain reaction amplification of n gene in patients with hantavirus cardiopulmonary syndrome. | we report a nested reverse transcription-polymerase chain reaction (rt-pcr) assay for hantavirus using primers selected to match high homology regions of hantavirus genomes detected from the whole blood of hantavirus cardiopulmonary syndrome (hcps) patients from brazil, also including the n gene nucleotide sequence of araraquara virus. hantavirus genomes were detected in eight out of nine blood samples from the hcps patients by rt-pcr (88.9% positivity) and in all 9 blood samples (100% positivit ... | 2004 | 15558177 |
| identifying rodent hantavirus reservoirs, brazil. | we describe the genetic analysis of samples from hantavirus pulmonary syndrome (hps) patients from southern and southeastern states of brazil and rodents captured at the presumed site of infection of these patients. a total of 65 samples that were antibody-positive for sin nombre or laguna negra virus by enzyme-linked immunosorbent assay were processed by nested reverse transcription-polymerase chain reaction (rt-pcr) by using several primer combinations in the m and s genome segments. pcr produ ... | 2004 | 15663849 |
| high rates of molecular evolution in hantaviruses. | hantaviruses are rodent-borne bunyaviruses that infect the arvicolinae, murinae, and sigmodontinae subfamilies of muridae. the rate of molecular evolution in the hantaviruses has been previously estimated at approximately 10(-7) nucleotide substitutions per site, per year (substitutions/site/year), based on the assumption of codivergence and hence shared divergence times with their rodent hosts. if substantiated, this would make the hantaviruses among the slowest evolving of all rna viruses. how ... | 2008 | 18417484 |
| expression of a hantavirus n protein and its efficacy as antigen in immune assays. | hantavirus cardiopulmonary syndrome (hcps) has been recognized as an important public heath problem. five hantaviruses associated with hcps are currently known in brazil: juquitiba, araraquara, laguna negra-like, castelo dos sonhos, and anajatuba viruses. the laboratory diagnosis of hcps is routinely carried out by the detection of anti-hantavirus igm and/or igg antibodies. the present study describes the expression of the n protein of a hantavirus detected in the blood sample of an hcps patient ... | 2008 | 18719741 |
| natural host relationships and genetic diversity of rodent-associated hantaviruses in southeastern brazil. | hantaviruses are rodent-borne rna viruses that have caused hantavirus cardiopulmonary syndrome in several brazilian regions. in the present study, geographical distribution, seroprevalence, natural host range, and phylogenetic relations of rodent-associated hantaviruses collected from seven counties of southeastern brazil were evaluated. | 2008 | 19001829 |
| hantavirus pulmonary syndrome, central plateau, southeastern, and southern brazil. | hantavirus pulmonary syndrome (hps) is an increasing health problem in brazil because of encroachment of sprawling urban, agricultural, and cattle-raising areas into habitats of subfamily sigmodontinae rodents, which serve as hantavirus reservoirs. from 1993 through june 2007, a total of 884 cases of hps were reported in brazil (case-fatality rate 39%). to better understand this emerging disease, we collected 89 human serum samples and 68 rodent lung samples containing antibodies to hantavirus f ... | 2009 | 19331732 |
| evaluation of an enzyme-linked immunosorbent assay based on araraquara virus recombinant nucleocapsid protein. | laboratory diagnosis of hantavirus cardiopulmonary syndrome (hcps) in brazil has been performed mostly by detection of igm antibodies to recombinant antigen purified from sin nombre virus and andes virus (andv). recently, a recombinant nucleocapsid (rn) protein of araraquara virus (arav), a brazilian hantavirus, was obtained in escherichia coli. to evaluate arav rn as antigen for antibody detection, serum samples from 30 patients from argentina seropositive for hantavirus were tested. all sample ... | 2009 | 19635882 |
| genetic characterization of a juquitiba-like viral lineage in oligoryzomys nigripes in rio de janeiro, brazil. | hantaviruses, family bunyaviridae, are rodent-borne rna viruses that have caused cases of hantavirus cardiopulmonary syndrome (hcps) in various regions of the americas. there are five hantaviral lineages associated with hcps in brazil: juquitiba virus (juqv), araraquara virus (arav), laguna negra-like virus (lnv), castelo dos sonhos virus (casv), and anajatuba virus (anajv). three additional hantaviruses have been described in rodents alone: rio mearim virus, jaborá virus, and a hantavirus linea ... | 2009 | 19660427 |
| diagnosis of hantavirus infection in humans and rodents in ribeirão preto, state of são paulo, brazil. | hantavirus pulmonary and cardiovascular syndrome (hpcs) is an emerging serious disease in the americas. hantaviruses (bunyaviridae) are the causative agents of this syndrome and are mainly transmitted through inhalation of aerosols containing the excreta of wild rodents. in the ribeirão preto region (state of são paulo, brazil), hpcs has been reported since 1998, caused by the araraquara virus (arav), for which necromys lasiurus is the rodent reservoir. this study aimed to show diagnostic result ... | 2010 | 20802928 |
| expression of recombinant araraquara hantavirus nucleoprotein in insect cells and its use as an antigen for immunodetection compared to the same antigen expressed in escherichia coli. | abstract: background: antigens for hantavirus serological tests have been produced using dna recombinant technology for more than twenty years. several different strategies have been used for that purpose. all of them avoid the risks and difficulties involved in multiplying hantavirus in the laboratory. in brazil, the araraquara virus is one of the main causes of hantavirus cardio-pulmonary syndrome (hcps). methods. in this investigation, we report the expression of the n protein of the araraqua ... | 2011 | 21569341 |
| serological survey of hantavirus in inhabitants from tropical and subtropical areas of brazil. | brazil has reported more than 1,600 cases of hantavirus cardiopulmonary syndrome (hps) since 1993, with a 39% rate of reported fatalities. using a recombinant nucleocapsid protein of araraquara virus, we performed elisa to detect igg antibodies against hantavirus in human sera. the aim of this study was to analyze hantavirus antibody levels in inhabitants from a tropical area (amazon region) in rondônia state and a subtropical (atlantic rain forest) region in são paulo state, brazil. a total of ... | 2016 | 27034670 |
| hantavirus pulmonary syndrome and rodent reservoirs in the savanna-like biome of brazil's southeastern region. | this paper describes the diversity of rodent fauna in an area endemic for hantavirus cardiopulmonary syndrome (hcps) in brazil, the population dynamics and the relationship of rodents with hantavirus in the cerrado (savanna-like) biome. additionally, an analysis is made of the partial s segment sequences of the hantaviruses obtained from serologically confirmed human hcps cases and from rodent specimens. rodents were collected during four campaigns. human serum samples were collected from suspec ... | 2016 | 26541807 |
| detection of different south american hantaviruses. | hantaviruses are the etiologic agents of hemorrhagic fever with renal syndrome (hfrs) in old world, and hantavirus pulmonary syndrome (hps)/hantavirus cardiopulmonary syndrome (hcps), in the new world. serological methods are the most common approach used for laboratory diagnosis of hcps, however theses methods do not allow the characterization of viral genotypes. the polymerase chain reaction (pcr) has been extensively used for diagnosis of viral infections, including those caused by hantavirus ... | 2015 | 26220480 |
| development of a novel plaque reduction neutralisation test for hantavirus infection. | in the americas, hantaviruses cause severe cardiopulmonary syndrome (hcps) with a high fatality rate. hantavirus infection is commonly diagnosed using serologic techniques and reverse transcription-polymerase chain reaction. this paper presents a novel plaque reduction neutralisation test (prnt) for detecting antibodies to brazilian hantavirus. using prnt, plaque detection was enhanced by adding 0.6% of dimethyl sulfoxide into the overlay culture medium of the infected cells. this procedure faci ... | 2015 | 26132430 |
| evidence of hantavirus infection among bats in brazil. | hantaviruses are zoonotic viruses harbored by rodents, bats, and shrews. at present, only rodent-borne hantaviruses are associated with severe illness in humans. new species of hantaviruses have been recently identified in bats and shrews greatly expanding the potential reservoirs and ranges of these viruses. brazil has one of the highest incidences of hantavirus cardiopulmonary syndrome in south america, hence it is critical to know what is the prevalence of hantaviruses in brazil. although muc ... | 2015 | 26078322 |
| co-circulation in a single biome of the juquitiba and araraquara hantavirus detected in human sera in a sub-tropical region of brazil. | hantaviruses is an emerging infectious disease. although hcps has been reported in several regions of brazil, more cases of hcps have recently been reported in minas gerais than in any other state. in 2009, we analyzed 27 samples presenting antibodies against hantaviruses. these samples originated from 688 symptomatic patients, as determined based on the hemorrhagic fever protocol. a subsequent sybr green-based real-time rt-pcr demonstrated the presence of the virus in 22 of the samples. among t ... | 2015 | 25678450 |
| polymorphisms in human leukocyte antigens, human platelet antigens, and cytokine genes in hantavirus cardiopulmonary syndrome patients from ribeirão preto, brazil. | hantavirus cardiopulmonary syndrome is a severe human disease associated with hantavirus infection. the clinical course of illness varies greatly among individuals, possibly due to viral and immunological elements and the influence of host genetic factors on clinical outcome. as the magnitude of immune activation has been associated with disease severity, polymorphisms in genes involved in the immune response that may affect the development of this syndrome were investigated. polymorphisms in th ... | 2014 | 24615895 |
| development of a one-step sybr green i real-time rt-pcr assay for the detection and quantitation of araraquara and rio mamore hantavirus. | hantaviruses are members of the family bunyaviridae and are an emerging cause of disease worldwide with high lethality in the americas. in brazil, the diagnosis for hantaviruses is based on immunologic techniques associated with conventional rt-pcr. a novel one-step sybr green real-time rt-pcr was developed for the detection and quantitation of araraquara (arav) and rio mamore hantavirus (riomv). the detection limit of assay was 10 copies/μl of rna in vitro transcribed of segment s. the specific ... | 2013 | 24056673 |
| serological survey of hantavirus in rodents in uberlândia, minas gerais, brazil. | we conducted a serological survey to determine the presence of hantavirus infection in rodents in uberlândia, minas gerais as well as to identify and characterize associated factors. rodents were captured using sherman live-capture traps set in rural and peri-urban environments. a total of 611 rodents were captured. there was a higher trap success in peri-urban areas (26.3%) and a higher prevalence of antibodies among rodents captured in rural areas (2.9%). necromys lasiurus was the most common ... | 2013 | 23740018 |
| prevalence of antibody to hantaviruses in humans and rodents in the caribbean region of colombia determined using araraquara and maciel virus antigens. | we tested sera from 286 agricultural workers and 322 rodents in the department of córdoba, northeastern colombia, for antibodies against two hantaviruses. the sera were analysed by indirect elisa using the lysate of vero e6 cells infected with maciel virus (macv) or the n protein of araraquara virus (arav) as antigens for the detection of antibodies against hantaviruses. twenty-four human sera were igg positive using one or both antigens. we detected anti-macv igg antibodies in 10 sera (3.5%) an ... | 2013 | 23579795 |
| detection of hantavirus in bats from remaining rain forest in são paulo, brazil. | the significant biodiversity found in brazil is a potential for the emergence of new zoonoses. study in some places of the world suggest of the presence to hantavirus in tissues of bats. researches of hantavirus in wildlife, out rodents, are very scarce in brazil. therefore we decided to investigate in tissues of different species of wild animals captured in the same region where rodents were detected positive for this virus. the present work analyzed ninety-one animals (64 rodents, 19 opossums, ... | 2012 | 23259834 |
| a retrospective serologic survey of hantavirus infections in the county of cássia dos coqueiros, state of são paulo, brazil. | in recent years, hantavirus infections producing severe diseases have obtained an increased attention from public health authorities from the countries of eurasia to the americas. brazil has reported 1,300 cases of hantavirus cardiopulmonary syndrome (hcps) from 1993 to 2010, with about 80 of them occurring in the northeast of the state of são paulo, with 48% fatality rate. araraquara virus was the causative agent of hcps in the region. considering that hantaviruses causing human disease in the ... | 2012 | 22767095 |
| co-circulation of araraquara and juquitiba hantavirus in brazilian cerrado. | hantavirus cardiopulmonary syndrome is an emerging serious disease in the americas, transmitted from wild rodents to humans through inhalation of aerosol containing virus. herein, we characterized two distinct hantaviruses circulating in rodent species form central plateau, midwestern region of brazil in the cerrado (savanna-like) biome, an area characterized by small trees and grasses adapted to climates with long dry periods. in this study, we identified the co-circulation of the araraquara vi ... | 2017 | 28856421 |