| sarcocystis and related organisms in australian wildlife: iii. sarcocystis murinotechis sp.n. life cycle in rats (rattus, pseudomys and mastocomys spp.) and tiger snakes (notechis ater). | sporocysts from tiger snakes (notechis ater) produced thick-walled sarcocysts in laboratory rats (rattus norvegicus). ultrastructurally these organisms were identical with sarcocysts found in native rats, but were different from sarcocystis singaporensis. sarocystis murinotechis sp.n. is proposed for this parasite of rodents and tiger snakes. when naturally-infected rats were fed to kittens (felis domestica), quolls (dasyurus viverrinus) and a masked owl (tyto novaehollandiae) no sporocysts were ... | 1980 | 6768904 |
| [the life-history of sarcocystis singaporensis zaman and colley, 1976 in the definitive and intermediate host (author's transl)]. | sporulated oocysts and sporocysts (9.3 x 7.3 micrometer) of sarcocystis singaporensis - isolated from freshly imported snakes (python reticulatus) - were fed to various animals to test their function as suitable intermediate hosts (nmri-mice, albino rats, meriones unguiculatus, golden hamsters, guinea pigs, mastomys natalensis, field-voles [microtus arvalis], pigeons, and chickens). only in rats were muscle-cysts formed. the optimal dose was 150 sporocysts per rat. two generations of merogony co ... | 1980 | 6771934 |
| sarcocystis singaporensis zaman and colley, (1975) 1976, sarcocystis villivilliso sp. n., and sarcocystis zamani sp. n.: development, morphology, and persistence in the laboratory rat, rattus norvegicus. | sporocysts obtained in singapore in the feces of the boid snake, python reticulatus, were given by mouth to laboratory rats, mice, and a monkey (macaca mulatta). infections developed in rats only. schizonts were observed in vascular endothelium of various tissues of rats examined at 7 to 15 days of infection; and in each of 20 rats examined at 1 mo to 2 yr, cysts of three species of sarcocystis were observed. based on observations made by light and electron microscopy, one species was identified ... | 1981 | 6787185 |
| sarcocystis singaporensis: studies on host specificity, pathogenicity, and potential use as a biocontrol agent of wild rats. | host specificity and pathogenicity of sarcocystis singaporensis were investigated as a prerequisite to a subsequent application of the parasite as a biocontrol agent of wild rats in egypt. after inoculation of 7 snake species comprising the families elapidae, viperidae, colubridae, and boidae with sarcocysts, sporocyst development was only observed in a reticulated python. among amphibians, reptiles, and rodents that orally received various sporocyst doses in the laboratory, 2 x 10(4) sporocysts ... | 1996 | 8604097 |
| in vitro cultivation of the vascular phase of sarcocystis singaporensis. | to establish an in vitro culture system for the precystic phase of sarcocystis singaporensis, we initially tested various excysting fluids for sporocysts. an excysting fluid containing 2.5% bovine taurocholate and 10% bile of the specific intermediate host, rattus norvegicus, in rpmi medium was the most suitable resulting in excystation of 80% of the sporozoites. subsequently, we identified brain endothelial cells and pneumonocytes of the rat to promote growth of sporozoites to schizonts. hepato ... | 1997 | 9225443 |
| identification of a subpopulation of merozoites of sarcocystis singaporensis that invades and partially develops inside muscle cells in vitro. | the affinity of merozoites of sarcocystis singaporensis obtained from the lungs of acutely infected rats to muscle cells and other cell lines grown in vitro was examined. two distinct types of mature schizonts developed in the lungs 11-13 days p.i. with sporocysts: those containing pas- merozoites (type 1) which mainly reacted with antibodies prepared against sporozoites, and others containing pas+ merozoites (type 2) which were antigenically close to bradyzoites. when inoculated onto cell cultu ... | 1999 | 10205799 |
| biological control of rodents using sarcocystis singaporensis. | parasites have been identified as an important factor in regulating vertebrate populations. in replicated field experiments (plots up to 4 ha) performed in thailand we tested whether commensal and field rodents could be artificially infected and controlled with the host-restricted apicomplexan protozoon sarcocystis singaporensis which is endemic in southeast asia. when bait-pellets containing high numbers of these parasites were consumed by rodents of three species (rattus norvegicus, rattus tio ... | 1999 | 10576581 |
| fine structure of the development of sarcocystis singaporensis in python reticulatus from macrogamont to sporulated oocyst stage. | three, 4-month old reticulated pythons (python reticulatus) hatched from eggs laid by a newly caught female from singapore island, were fed on muscles of sarcocystis singaporensis-infected rattus rattus caught in singapore. snakes were sacrificed five, six and eight days later. the infected tissues were studied by transmission electron microscope. the present communication summarizes findings on macrogamont and oocyst stages. in the premature stages, rough endoplasmic reticulum consolidate into ... | 2000 | 11031755 |
| effects of sequence alignment and structural domains of ribosomal dna on phylogeny reconstruction for the protozoan family sarcocystidae. | finding correct species relationships using phylogeny reconstruction based on molecular data is dependent on several empirical and technical factors. these include the choice of dna sequence from which phylogeny is to be inferred, the establishment of character homology within a sequence alignment, and the phylogeny algorithm used. nevertheless, sequencing and phylogeny tools provide a way of testing certain hypotheses regarding the relationship among the organisms for which phenotypic character ... | 2000 | 11110900 |
| fine structural development of microgamonts of sarcocystis singaporensis in python reticulatus. | three 4-month-old reticulated pythons (python reticulatus), hatched from eggs laid by a newly caught female from singapore island, were fed on muscles of sarcocystis singaporensis-infected rattus rattus caught in singapore. snakes were sacrificed 5, 6 and 8 days later, the infected tissues were studied by transmission electron microscopy. the present communication summarizes findings on microgamont stages. both premature and mature microgamonts were already present in the snake sacrificed 5 days ... | 2000 | 11133107 |
| immunoglobulin subclass responses of wild brown rats to sarcocystis singaporensis. | immunoglobulin subclass responses of wild brown rats (rattus norvegicus) from southeastern asia to the endemic cyst-forming coccidian sarcocystis singaporensis were characterised. the antibody response of brown rats to wild-type parasites (high reproductive capacity) showed a th1 profile during acute infection, namely elevated concentrations of parasite-specific igg2b and igg2c and absence of igg1. chronic infection (bradyzoite development) resulted in a mixed th1/th2 pattern whereby significant ... | 2001 | 11226454 |
| binding of a monoclonal antibody to sporozoites of sarcocystis singaporensis enhances escape from the parasitophorous vacuole, which is necessary for intracellular development. | early intracellular development in vitro of the cyst-forming protozoon sarcocystis singaporensis and the influence of a monoclonal antibody on invasion, intracellular localization, and development of sporozoites were studied. as revealed by immunofluorescence using parasite-specific antibodies which labeled the parasitophorous vacuole membrane (pvm) and by ultrastructural analysis, sporozoites invaded pneumonocytes of the rat via formation of a parasitophorous vacuole (pv). about half of the spo ... | 2001 | 11553592 |
| reduction of transmission stages concomitant with increased host immune responses to hypervirulent sarcocystis singaporensis, and natural selection for intermediate virulence. | parasite virulence (pathogenicity depending on inoculum size) and host immune reactions were examined for the apicomplexan protozoan sarcocystis singaporensis. this parasite is endemic in southeastern asia and multiplies as a proliferation (merozoite) and transmission stage (bradyzoite) in rats. virulence in wild brown rats of parasites freshly isolated in the wild (wild-type) was surprisingly constant within the endemic area and showed an intermediate level. in contrast, serially passaged paras ... | 2001 | 11730791 |
| fine structure of sarcocystis singaporensis merogony stages preceding sarcocyst formation in the rat. | the fine structure of merogony stages of sarcocystis singaporensis (zaman and colley, 1975) is described from experimentally infected laboratory rats, 10 days after being fed sporocysts obtained from naturally infected python reticulatus from singapore. infection was shown to consist exclusively of s. singaporensis. parasites developed in the endothelial cells of the lungs, brain, kidney and heart. infection comprised meronts prior to division, dividing and divided meronts, and dispersed merozoi ... | 2002 | 11822741 |
| ultrastructural study of the development of sarcocystis singaporensis sarcocysts in the muscles of its rat host. | laboratory rats fed sporocysts of sarcocystis singaporensis (zaman & colley, 1975) zaman & colley, 1976 originating from singapore were euthanized 22, 23, 33 and 80 days later. sporocysts were extracted from feces of either naturally or laboratory-infected python reticulatus. electron microscopically examined longue and esophageal muscles yielded images of successive developing stages of sarcocysts. the primary wall evolved from a continuous thin layer into folds and later, into villar protrusio ... | 2002 | 12116862 |
| phylogeny and sequence variability of the sarcocystis singaporensis zaman and colley, (1975) 1976 ssrdna. | the coccidium sarcocystis singaporensis (apicomplexa: sarcocystidae) is a cyst-forming parasite with potential as a biological agent for the control of wild populations of rodents in non-native environments. phylogenetic analysis based on the ssrdna supports s. singaporensisisolates as a sister species to sarcosporidians transmitted between snakes and rodents but an association with the carnivore-ruminant sarcocystis spp. could not be rejected by likelihood ratio tests. four complete and six par ... | 2002 | 12172812 |
| molecular identification of sarcocystis spp. helped to define the origin of green pythons (morelia viridis) confiscated in germany. | sarcocystis spp. represent apicomplexan parasites. they usually have a heteroxenous life cycle. around 200 species have been described, affecting a wide range of animals worldwide, including reptiles. in recent years, large numbers of reptiles have been imported into europe as pets and, as a consequence, animal welfare and species protection issues emerged. a sample of pooled feces from four confiscated green pythons (morelia viridis) containing sarcocystis spp. sporocysts was investigated. thes ... | 2014 | 24476633 |
| molecular characterization of the ribosomal dna unit of sarcocystis singaporensis, sarcocystis zamani and sarcocystis zuoi from rodents in thailand. | sarcocystis species are heteroxenous cyst-forming coccidian protozoan parasites with a wide host range, including rodents. in this study, sarcocystis spp. samples were isolated from bandicota indica, rattus argentiventer, r. tiomanicus and r. norvegicus across five provinces of thailand. two major groups of sarcocystis cysts were determined in this study: large and small cysts. by sequence comparisons and phylogenetic analyses based on the partial sequences of 28s ribosomal dna, the large cysts ... | 2017 | 28701623 |