genome size variation in the pine fusiform rust pathogen cronartium quercuum f.sp. fusiforme as determined by flow cytometry.the genome size of the pine fusiform rust pathogen cronartium quercuum f.sp. fusiforme (cqf) was determined by flow cytometric analysis of propidium iodide-stained, intact haploid pycniospores with haploid spores of two genetically well characterized fungal species, sclerotinia sclerotiorum and puccinia graminis f.sp. tritici, as size standards. the cqf haploid genome was estimated at ~90 mb, similar to other pucciniales species for which reference genome sequences are available. twenty-three cq ...201020943541
a genomic map enriched for markers linked to avr1 in cronartium quercuum f.sp. fusiforme.a novel approach is presented to map avirulence gene avr1 in the basidiomycete cronartium quercuum f.sp. fusiforme, the causal agent of fusiform rust disease in pines. dna markers tightly linked to resistance gene fr1 in loblolly pine tree 10-5 were used to classify 10-5 seedling progeny as either resistant or susceptible. a single dikaryotic isolate (p2) heterozygous at the corresponding avr1 gene was developed by crossing fr1 avirulent isolate sc20-21 with fr1 virulent isolate nc2-40. bulk bas ...201020888926
micrornas in loblolly pine (pinus taeda l.) and their association with fusiform rust gall development.micrornas (mirnas) are endogenous small rnas that can have large-scale regulatory effects on development and on stress responses in plants. the endemic rust fungus cronartium quercuum f. sp. fusiforme causes fusiform rust disease in pines, resulting in the development of spindle-shaped galls (cankers) on branches or stems. this disease is the most destructive disease of pines in the southern usa. to test whether mirnas play roles in fusiform rust gall development, we cloned and identified 26 mir ...200717635765
differential expression of pine and cronartium quercuum f. sp. fusiforme genes in fusiform rust galls.cronartium quercuum f. sp. fusiforme is the causative agent of fusiform rust disease of southern pines in the united states. this disease is characterized by the formation of woody branch and stem galls. differential display was used to identify pine genes whose expression is altered by c. quercuum f. sp. fusiforme infection and to identify c. quercuum f. sp. fusiforme genes that are expressed in fusiform rust galls. six pine cdnas that appeared to be differentially expressed in galled and healt ...200414711673
microsatellite dna suggests regional structure in the fusiform rust fungus cronartium quercuum f. sp fusiforme.this paper reports results obtained from microsatellite dna analysis of genetic structure for populations of the native fungus cronartium quercuum f. sp fusiforme infecting loblolly pine (pinus taeda l.) over much of this host's natural range. mostly all fusiform rust galls formed under field conditions are produced as a result of infection and colonization by haploid mycelium originating from a single basidiospore of c. quercuum fusiforme. if multiple infections do occur, then only a single hap ...200413130304
genetic diversity in cronartium quercuum f.sp. fusiforme on loblolly pines in southern u.s.twelve random amplified polymorphic dna (rapd) markers were used to study genetic variability in three populations of cronartium quercuum f.sp.fusiforme, the causal agent of fusiform rust on pines. most of the variability (94%) was found within populations while 6% of the total variability was attributable to frequency differences between populations. the frequency of three markers was significantly different between regions of origin on an east-west transect. three markers were completely absen ...19947882431
components of partial resistance in the slash pine-fusiform rust pathosystem.abstract progeny of rust-resistant, open-pollinated slash pine families exhibited components of partial resistance in greenhouse tests. nine-month-old seedlings of some resistant families had (i) a greater frequency of short galls (</= 25 mm long), and (ii) fewer sporulating galls compared with other resistant and susceptible families when exposed to cronartium quercuum f. sp. fusiforme in greenhouse tests. these components of partial resistance were evident with (i) mixed- or single-gall inocul ...200018944526
genetic interaction of the fusiform rust fungus with resistance gene fr1 in loblolly pine.abstract we propose a method for defining dna markers linked to cronartium quercuum f. sp. fusiforme avirulence (avr) genes. however, before this method can be successfully employed, a spore competition study was needed to determine the genetic composition of single pycnial drops and multiple drops on single galls when using the standard inoculation procedure, whether virulent (avr1) basidiospores ever predispose some resistant (fr1/fr1) trees to infection by avirulent (avr1) basidiospores, and ...200518943039
genetic dissection of fusiform rust and pitch canker disease traits in loblolly pine.loblolly pine (pinus taeda l.) exhibits genetic resistance to fusiform rust disease (incited by the biotrophic fungus, cronartium quercuum f. sp. fusiforme) and pitch canker disease (incited by the necrotrophic fungus, fusarium circinatum). in this study, a total of 14,015 loblolly pine cuttings from 1,065 clones were screened in controlled greenhouse conditions to identify phenotypes of clones, families, and parents that guide a genetic dissection of disease traits associated with pitch canker ...200515700146
pine genes regulated by the necrotrophic pathogen fusarium circinatum.a targeted genomics approach was used to construct a cdna array of potential pathogen-regulated genes for investigating host-pathogen interactions in pine trees (pinus species). this array, containing a nonredundant set of 311 cdnas, was assembled by combining smaller sets of cdnas generated by differential display or suppression subtraction hybridization using a variety of pathogen treatments and elicitors. the array was probed to identify host genes regulated by fusarium circinatum, a necrotro ...200415221139
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