presence of a lethal protease in the extracellular products of vibrio splendidus-vibrio lentus related strains.the presence of a lethal extracellular 39-kda protease, a virulence determinant of a listonella pelagia strain which produces vibriosis in turbot, was determined in the extracellular products (ecp) of 33 vibrionaceae strains. both immunological and enzymatic techniques distinguished this specific protease from other vibrionaceae proteins. it was detected in 15% (5/33) of the ecps assayed belonging to strains of the vibrio splendidus-v. lentus related group isolated in galician aquaculture system ...200617169102
vibrio lentus associated with diseased wild octopus (octopus vulgaris).studies were undertaken to identify the bacteria involved in a disease of wild octopus (octopus vulgaris). signs of the disease include round hard lesions in the arms or head mantle, leading, in advanced cases, to the loss of skin and the exposure of the muscle beneath. bacterial strains isolated from sterile organs have been evaluated taxonomically and by experimental infections. different phenotypes and ribotypes of vibrio lentus were identified. experimental infection by bath challenge demons ...200312788284
effects of three strains of intestinal autochthonous bacteria and their extracellular products on the immune response and disease resistance of common carp, cyprinus carpio.the study isolated three strains of intestinal autochthonous bacteria aeromonas veronii ba-1, vibrio lentus ba-2, and flavobacterium sasangense ba-3 from the intestinal tract of the common carp (cyprinus carpio). to reveal the effects of these three strains of bacteria on the innate immunity of carp, the lysozyme, complement c3, total serum protein, albumin and globulin levels, respiratory burst activity, phagocytic activity by blood leucocytes and the expression of il-1b, lysozyme-c, and tnf-α ...201424161775
vibrio lentus sp. nov., isolated from mediterranean oysters.twelve phenotypically similar marine bacteria have been studied by means of ribotyping, dna-dna hybridization and cultural and physiological characterization. phylogenetic analysis has been performed of the 16s and 23s rrna genes of two representative strains. phylogenetically, they belong to the vibrio/photobacterium branch of the gamma-proteobacteria and they share all of the properties that define the genus vibrio. the strains represent a new vibrio species that is phenotypically similar to v ...200111491345
vibrio lentus protects gnotobiotic sea bass (dicentrarchus labrax l.) larvae against challenge with vibrio harveyi.due to the mounting awareness of the risks associated with the use of antibiotics in aquaculture, treatment with probiotics has recently emerged as the preferred environmental-friendly prophylactic approach in marine larviculture. however, the presence of unknown and variable microbiota in fish larvae makes it impossible to disentangle the efficacy of treatment with probiotics. in this respect, the recent development of a germ-free culture model for european sea bass (dicentrarchus labrax l.) la ...201626931390
cellular and transcriptomic response to treatment with the probiotic candidate vibrio lentus in gnotobiotic sea bass (dicentrarchus labrax) larvae.the present study aimed at evaluating the cellular and transcriptomic responses induced by the probiotic candidate vibrio lentus with gnotobiotic european sea bass (dicentrarchus labrax, linnaeus 1785) larvae. for this, a histomorphological analysis was performed using the terminal deoxynucleotidyltransferase-mediated dutp nick end labeling (tunel) and the anti-proliferating cell nuclear antigen (pcna) assay. in addition, a global transcriptomic approach was adopted to study the whole body mrna ...201728119147
selection of reliable reference genes for rt-qpcr studies in octopus vulgaris paralarvae during development and immune-stimulation.the common octopus, octopus vulgaris is a new candidate species for aquaculture. however, rearing of octopus paralarvae is hampered by high mortality and poor growth rates that impede its entire culture. the study of genes involved in the octopus development and immune response capability could help to understand the key of paralarvae survival and thus, to complete the octopus life cycle. quantitative real-time pcr (rt-qpcr) is the most frequently tool used to quantify the gene expression becaus ...201627267177
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