| nucleotide sequence, genome organization and phylogenetic analysis of pineapple mealybug wilt-associated virus-2. | the genome of pineapple mealybug wilt-associated closterovirus-2 (pmwav-2) was cloned from double-stranded rna isolated from diseased pineapple and its sequence determined. the 3'-terminal 14861 nt of the single-stranded rna genome contains ten open reading frames (orfs) which, from 5' to 3', potentially encode a >204 kda polyprotein containing papain-like protease, methyltransferase and helicase domains (orf1a), a 65 kda rna-dependent rna polymerase (orf1b), a 5 kda hydrophobic protein (orf2), ... | 2001 | 11125151 |
| genome organization and phylogenetic relationship of pineapple mealybug wilt associated virus-3 with family closteroviridae members. | the nucleotide sequence of pineapple mealybug wilt associated virus-3 (pmwav-3) (closteroviridae: ampelovirus), spanning seven open reading frames (orfs) and the untranslatable region of the 3' end was determined. based on the amino acid identities with orthologous orfs of pmwav-1 (54%-73%) and pmwav-2 (13%-35%), we propose pmwav-3 is a new species in the pmwav complex. pmwav-3 lacks an intergenic region between orf1b and orf2, encodes a relatively small, 28.8 kda, coat protein, and lacks a coat ... | 2009 | 19225875 |
| analysis of pineapple mealybug wilt associated virus -1 and -2 for potential rna silencing suppressors and pathogenicity factors. | higher plants use rna silencing to defend against viral infections. as a counter defense, plant viruses have evolved proteins that suppress rna silencing. mealybug wilt of pineapple (mwp), an important disease of pineapple, has been associated with at least three distinct viruses, pineapple mealybug wilt associated virus -1, -2, and -3 (pmwav-1, -2, and -3). selected open reading frames (orfs) of pmwav-1 and pmwav-2 were screened for their local and systemic suppressor activities in agrobacteriu ... | 2015 | 25751306 |
| closterovirus infection and mealybug exposure are necessary for the development of mealybug wilt of pineapple disease. | abstract the roles of pineapple mealybug wilt-associated viruses (pmwavs) and mealybug (dysmicoccus spp.) feeding in the etiology of mealybug wilt of pineapple (mwp) were evaluated. container-grown pineapple (ananas comosus) plants from five commercially grown hawaiian proprietary selections and a field study utilizing a randomized complete block design were used to test four treatments for induction of mwp: pmwav-1-free and pmwav-1-infected plants maintained mealybug-free, and pmwav-1-free and ... | 2002 | 18944017 |
| a highly sensitive single-tube nested pcr assay for the detection of pineapple mealybug wilt associated virus-2 (pmwav-2). | an assay was developed for the detection of pineapple mealybug wilt associated virus-2 (pmwav-2), an important factor in the etiology of mealybug wilt of pineapple. the assay combines reverse transcription of rna isolated from pineapple with a specific and very sensitive, single, closed-tube nested polymerase chain reaction (pcr) to amplify a segment of the coat protein gene of the pmwav-2. the outer primers were designed to anneal at higher temperatures than the nested primers to prevent primer ... | 2012 | 22484612 |