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bacillus endophyticus sp. nov., isolated from the inner tissues of cotton plants (gossypium sp.).four strains of aerobic, endospore-forming bacteria were isolated from the inner tissues of healthy cotton plants (gossypium sp., dushanbe, tajikistan). the organisms had identical randomly amplified polymorphic dna patterns that distinguished them from other bacilli that are commonly isolated from plant tissues, e.g. bacillus amyloliquefaciens, bacillus licheniformis, bacillus pumilus and bacillus subtilis. pcr amplification of 16s-23s rrna spacer regions suggested that the four strains could b ...200211837291
protection of oilseed rape (brassica napus) toward fungal pathogens by strains of plant-associated bacillus amyloliquefaciens.in this report, four bacillus strains were tested for effects on plant fitness and disease protection of oilseed rape (brassica napus). the strains belonged to newly discovered plant-associated bacillus amyloliquefaciens and a recently proposed species, bacillus endophyticus. the fungal pathogens tested represented different infection strategies and included alternaria brassicae, botrytis cinerea, leptosphaeria maculans, and verticillium longisporum. the b. amyloliquefaciens strains showed no or ...200717186140
bacillamides from a hypersaline microbial mat bacterium.chemical studies of a bacillus endophyticus isolated from a bahamian hypersaline microbial mat led to the isolation of bacillamides b and c, new tryptamide thiazole metabolites. bioassay-guided fractionation using a hplc-uv-ms bioassay technique enabled the detection of these trace fermentation products, and their total structures were elucidated by combined spectroscopic techniques.200717988095
phytoremediation of metals from fly ash through bacterial augmentation.different combinations of four bacterial strains isolated from fly ash were used by us to study their impact on phytoextraction of metals from fly ash by brassica juncea grown in fly ash amended with farm yard manure (50:50 w/w). out of 11 bacterial consortia, a combination of two strains i.e. paenibacillus macerans nbrft5 + bacillus pumilus nbrft9 (c7) inoculated in the rhizosphere was found to enhance pb accumulation maximally by 278%, mn by 75%, zn by 163%, cr by 226% and ni by 414% compared ...201021080221
A Rapid Microbioassay for Discovery of Antagonistic Bacteria for Phytophthora parasitica var. nicotianae.A simple, rapid, small-scale microbioassay for infection of tobacco seedlings by Phytophthora parasitica var. nicotianae was developed here. This assay uses tobacco seedlings cultivated in Petri dishes for a standardized method for quantitation of initial zoospore inocula and high-throughput screening of antagonistic bacteria. Zoospore inocula between 102 to 105 spores per Petri dish were inoculated on 14-day-old tobacco seedlings for the susceptibility test. One thousand zoospores' treatment wa ...201122046969
stimulated phytoextraction of metals from fly ash by microbial interventions.various combinations of fly ash tolerant bacteria isolated from the rhizospheric zone of typha latifolia naturally growing on a fly ash dump site were tested for enhanced metal uptake by brassica juncea grown in fly ash amended with press mud. after enrichment of the bacteria in a nutrient broth, they were subsequently applied to the rhizospheric zone of b. juncea in different combinations. when the metal analysis was done in the plants at their maturity, it was revealed that out of 11 bacterial ...201223393983
draft genome sequences of four plant probiotic bacillus strains.here, we report the whole-genome sequences of four bacillus strains that exhibit plant probiotic activities. three of them are the type strains of bacillus endophyticus, "bacillus gaemokensis," and bacillus trypoxylicola, and the other, bacillus sp. strain kctc 13219, should be reclassified into a species belonging to the genus lysinibacillus.201627174273
isolation of endophytic plant growth-promoting bacteria associated with the halophyte salicornia europaea and evaluation of their promoting activity under salt stress.several reports have highlighted that many plant growth-promoting endophytic bacteria (pgpe) can assist their host plants in coping with various biotic and abiotic stresses. however, information about the pgpe colonizing in the halophytes is still scarce. this study was designed to isolate and characterize pgpe from salt-accumulating halophyte salicornia europaea grown under extreme salinity and to evaluate in vitro the bacterial mechanisms related to plant growth promotion. a total of 105 isola ...201627447799
potentiality of eisenia fetida to degrade disposable paper cups-an ecofriendly solution to solid waste pollution.the aim of the present study was to subject the post-consumer waste, namely paper cups for vermicomposting along with cow dung in three different ratios for a period of 90-140 days employing eisenia fetida. the post-consumer wastes are a menace in many developing countries including india. this waste was provided as feed for earthworms and was converted to vermicompost. vermicompost prepared with paper cup waste was analyzed for their physicochemical properties. based on the physicochemical prop ...201525220767
termitarium-inhabiting bacillus spp. enhanced plant growth and bioactive component in turmeric (curcuma longa l.).curcumin (diferuloyl methane) is the main bioactive component of turmeric (curcuma longa l.) having remarkable multipotent medicinal and therapeutic applications. two bacilli isolated from termitarium soil and identified as bacillus endophyticus tsh42 and bacillus cereus tsh77 were used for bacterization of rhizome for raising c. longa ver. suguna for growth and enhancement. both the strains showed remarkable pgp activities and also chemotactic in nature with high chemotactic index. turmeric pla ...201727913879
termitarium-inhabiting bacillus endophyticus tsh42 and bacillus cereus tsh77 colonizing curcuma longa l.: isolation, characterization, and evaluation of their biocontrol and plant-growth-promoting activities.bacillus strains were isolated from termitarium soil and screened for their antifungal activity through the production of diffusible and volatile metabolites. further, the bacterial strains that showed antifungal activity were evaluated for their biocontrol potential on the basis of their plant-growth-promoting attributes. termitarium-inhabiting bacillus strains tsh42 and tsh77 significantly reduced the growth of pathogenic fungus fusarium solani, controlled the symptoms of rhizome rot in turmer ...201627604298
genome sequence of bacillus endophyticus and analysis of its companion mechanism in the ketogulonigenium vulgare-bacillus strain consortium.bacillus strains have been widely used as the companion strain of ketogulonigenium vulgare in the process of vitamin c fermentation. different bacillus strains generate different effects on the growth of k. vulgare and ultimately influence the productivity. first, we identified that bacillus endophyticus hbe603 was an appropriate strain to cooperate with k. vulgare and the product conversion rate exceeded 90% in industrial vitamin c fermentation. here, we report the genome sequencing of the b. e ...201526248285
reconstruction of amino acid biosynthetic pathways increases the productivity of 2-keto-l-gulonic acid in ketogulonicigenium vulgare-bacillus endophyticus consortium via genes screening.defect in the amino acid biosynthetic pathways of ketogulonicigenium vulgare, the producing strain for 2-keto-l-gulonic acid (2-kga), is the key reason for its poor growth and low productivity. in this study, five different strains were firstly reconstructed by expressing absent genes in threonine, proline and histidine biosynthetic pathways for better 2-kga productivity. when mono-cultured in the shake flasks, the strain sybe_kv02080002 expressing hsk from gluconobacter oxydans in threonine bio ...201728283955
biodegradation of 2,4-dichlorophenol in packed-bed biofilm reactor: effect of hydraulic retention time, biogenic substrate, and loading rate.the removal of 2,4-dichlorophenol (2,4-dcp) by a pure culture of bacillus endophyticus strain immobilized on ceramic balls was studied in a packed bed biofilm reactor (pbbr). the biodegradation of 2,4-dcp was studied in fed-batch and continuous mode and the effect of different parameters such as hydraulic retention time (hrt), biogenetic substrate concentration, and loading rate on the removal of 2,4-dcp were evaluated. field emission scanning electron microscope (fesem) results established the ...201628061932
comparative analysis of l-sorbose dehydrogenase by docking strategy for 2-keto-l-gulonic acid production in ketogulonicigenium vulgare and bacillus endophyticus consortium.improving the yield of 2-keto-l-gulonic acid (2-kga), the direct precursor of vitamin c, draws more and more attention in industrial production. in this study, we try to increase the 2-kga productivity by computer-aided selection of genes encoding l-sorbose dehydrogenases (sdh) of ketogulonicigenium vulgare. first, six sdhs were modeled by docking strategy to predict the binding mode with co-factor pqq. the binding energy between ssda1-h/ssda1-l and pqq was the highest, followed by ssda3/ssda2. ...201627565673
comparative genomics analysis of the companion mechanisms of bacillus thuringiensis bc601 and bacillus endophyticus hbe603 in bacterial consortium.bacillus thuringiensis and bacillus endophyticus both act as the companion bacteria, which cooperate with ketogulonigenium vulgare in vitamin c two-step fermentation. two bacillus species have different morphologies, swarming motility and 2-keto-l-gulonic acid productivities when they co-culture with k. vulgare. here, we report the complete genome sequencing of b. thuringiensis bc601 and eight plasmids of b. endophyticus hbe603, and carry out the comparative genomics analysis. consequently, b. t ...201627353048
differential-display reverse transcription-pcr (ddrt-pcr): a new technology for molecular detection and studying one of the antagonistic factors of bacillus endophyticus strain sa against staphylococcus aureus (mrsa).differential display (ddrt-pcr) is a powerful technique for analyzing differences in gene expression. in-vivo expression technologies and differential display rt-pcr are providing new approaches to further examine a microbe's response to experimental conditions which more closely resemble natural microbial associations and habitats. in this study, bacillus endophyticus strain sa isolated from the inner tissue of the stem of the cultivated plant (salvadora persica, asir, kingdom of saudi arabia) ...201628330192
molecular diversity and multifarious plant growth promoting attributes of bacilli associated with wheat (triticum aestivum l.) rhizosphere from six diverse agro-ecological zones of india.the diversity of culturable bacilli was investigated in six wheat cultivating agro-ecological zones of india viz: northern hills, north western plains, north eastern plains, central, peninsular, and southern hills. these agro-ecological regions are based on the climatic conditions such as ph, salinity, drought, and temperature. a total of 395 bacilli were isolated by heat enrichment and different growth media. amplified ribosomal dna restriction analysis using three restriction enzymes alui, msp ...201626567901
isolation and identification of culturable bacteria from honeydew of whitefly, bemisia tabaci (g.) (hemiptera: aleyrodidae).bemisia tabaci (g.) is an important pest and a vector of gemini viruses infecting plants. during the process of feeding b. tabaci excretes honeydew which is rich in nutrients, and an excellent medium for microbial growth. recent report proved that volatile emitted by the honeydew associated bacteria of aphid, acyrthosiphon pisum harris was involved in natural enemy calling. thus understanding the honeydew associated bacteria is of paramount importance from the non-chemical method of insect pest ...201425606395
bacillus filamentosus sp. nov., isolated from sediment sample.a novel gram-stain positive, endospore-forming bacterium, designated sgd-14(t), was isolated from a marine sediment sample in goa province, india. cells of the isolate were found to be strictly aerobic. phylogenetic analysis based on 16s rrna gene sequences indicated that strain sgd-14(t) showed a similarity of 99.5 % with bacillus endophyticus and similarities to other bacillus type strains were below 96 %. the whole-cell sugar pattern was found to consist of ribose, xylose and glucose. the pre ...201525432072
induced phytoremediation of metals from fly ash mediated by plant growth promoting rhizobacteria.a study was carried out to observe the impact of a consortium of bacteria isolated from the fly ash on the metal accumulation by t. latifolia. when a consortium of bacteria bacillus endophyticus nbrft4 (mtcc 9021), paenibacillus macerans nbrfts (mtcc 8912) and bacillus pumilus nbrft9 (mtcc 8913) was bioaugmented into the rhizosphere of t. latifolia, it enhanced the metal concentration in root, stem and leaves of the plants through increased bioavailability of metals fe, cd, pb, cr, ni, cu and zn ...201324640248
bacillus oceani sp. nov., a new slightly halophilic bacterium, isolated from a deep sea sediment environment.a strictly aerobic, gram-stain positive, slightly halophilic strain, designated scsio 04524(t), was isolated from a deep sea sediment sample collected from the northern south china sea at a depth of 3415 m. the isolate slightly embedded into the medium after 72 h incubation at 30 °c. growth was found to occur on media with 0-10 % nacl but extremely weak growth occurred without supplying nacl. the predominant menaquinone was determined to be mk-7. the major cellular fatty acid identified was iso- ...201323934481
probiotics in the intestinal tract of juvenile whiteleg shrimp litopenaeus vannamei: modulation of the bacterial community.molecular analysis of the 16s rdna of the intestinal microbiota of whiteleg shrimp litopenaeus vannamei was examined to investigate the effect of a bacillus mix (bacillus endophyticus yc3-b, bacillus endophyticus c2-2, bacillus tequilensisyc5-2) and the commercial probiotic (alibio(®)) on intestinal bacterial communities and resistance to vibrio infection. pcr and single strain conformation polymorphism (sscp) analyses were then performed on dna extracted directly from guts. injection of shrimp ...201323161451
draft genome sequence of bacillus endophyticus 2102.bacillus endophyticus 2102 is an endospore-forming, plant growth-promoting rhizobacterium isolated from a hypersaline pond in south korea. here we present the draft sequence of b. endophyticus 2102, which is of interest because of its potential use in the industrial production of algaecides and bioplastics and for the treatment of industrial textile effluents.201223012284
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