| multiplex rt-pcr detection of four aphid-borne strawberry viruses in fragaria spp. in combination with a plant mrna specific internal control. | the principal aphid-borne viruses infecting strawberry (fragaria spp.) strawberry crinkle virus (scv), strawberry mild yellow edge virus (smyev), strawberry mottle virus (smov) and strawberry vein banding virus (svbv) can cause serious crop losses. in this paper, a multiplex reverse transcriptase polymerase chain reaction (rt-pcr) method is described for the simultaneous detection of all four viruses in combination with a plant mrna specific internal control which can be used as an indicator of ... | 2003 | 12880923 |
| genetic characterization of novel putative rhabdovirus and dsrna virus from japanese persimmon. | deep-sequencing analysis of nucleic acids from leaf tissue of japanese persimmon trees exhibiting fruit apex disorder in some fruits detected two molecules that were graft transmitted to healthy seedlings. one of the complete genomes consisted of 13 467 nt and encoded six genes similar to those of plant rhabdoviruses. the virus formed a distinct cluster in the genus cytorhabdovirus with lettuce necrotic yellows virus, lettuce yellow mottle virus and strawberry crinkle virus in a phylogenetic tre ... | 2013 | 23704275 |
| serial passage of strawberry crinkle virus in the aphid chaetosiphon jacobi. | strawberry crinkle virus was maintained through 6 consecutive serial passages by needle inoculation of the aphid vector, chaetosiphon jacobi. a dilution factor of at least 10(-3) at each passage, coupled with a high rate of successful inoculation (ca. 90%) and a stable incubation period, indicated that the virus titer was being maintained by multiplication in the aphid. | 1974 | 18620233 |
| strawberry crinkle virus, a cytorhabdovirus needing more attention from virologists. | summary taxonomic relationship: a member of nonsegmented, negative-sense, single-stranded rna viruses of the genus cytorhabdovirus (type member: lettuce necrotic yellows virus), family rhabdoviridae, order mononegavirales. members of the family rhabdoviridae can infect vertebrates, invertebrates and plants. physical properties: virions are bacilliform, 74-88 nm in diameter and 163-383 nm in length with surface projections probably composed of trimers of the glycoprotein g, occurring in the cytop ... | 2000 | 20572980 |
| completion of the genome sequence of lettuce necrotic yellows virus, type species of the genus cytorhabdovirus. | we completed the genome sequence of lettuce necrotic yellows virus (lnyv) by determining the nucleotide sequences of the 4a (putative phosphoprotein), 4b, m (matrix protein), g (glycoprotein) and l (polymerase) genes. the genome consists of 12,807 nucleotides and encodes six genes in the order 3' leader-n-4a(p)-4b-m-g-l-5' trailer. sequences were derived from clones of a cdna library from lnyv genomic rna and from fragments amplified using reverse transcription-polymerase chain reaction. the 4a ... | 2006 | 16313992 |