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observations on the life-cycle of the strigeoid trematode, apatemon (apatemon) gracilis (rudolphi, 1819) szidat, 1928.the life-cycle of apatemon (a.) gracilis was completed in the laboratory. the snail host is lymnaea peregra (muller). the cercaria is redescribed from a wide range of material. metacercariae were found in naturally infected rainbow trout (salmo gairdneri richardson), three-spined sticklebacks (gasterosteus aculeatus l.) and stone loach (nemacheilus barbatulus (l.)) from scotland and in three-spined sticklebacks from iceland. in trout, most metacercariae were found in the pericardial cavity, in s ...1976965706
lipovitellin-phosvitin crystals with orthorhombic features: thin-section electron microscopy, gel electrophoresis, and microanalysis in teleost and amphibian yolk platelets and a comparison with other vertebrates.yolk-platelet crystals in the teleosts pelvicachromis pulcher and noemacheilus barbatulus and the amphibians xenopus laevis, rana temporaria, r. esculenta, and triturus sp. have been studied by electron diffraction and imaging using a standardized processing (glutaraldehyde-osmium tetroxide fixation, glutaraldehyde-urea embedding, thin-section staining), by x-ray microanalysis, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of their constituents. the crystal lattice is orthorhombi ...19836602894
observations on the development of syncuaria squamata (nematoda: acuariidae), a parasite of cormorants, in the intermediate and paratenic hosts.the development of the nematode syncuaria squamata (linstow, 1883), a gizzard parasite of cormorants, was experimentally studied in the ostracod notodromas monacha. after the eggs of this nematode have been swallowed by the ostracod, the toothed first-stage larvae of the parasite are released and penetrate through the intestinal wall into the haemocoel of the crustacean. before attaining the infective third stage, the larvae moult twice in the body of the intermediate host (9-11 and 13-15 days a ...19947883250
colonization and establishment of pomphorhynchus laevis (acanthocephala) in an isolated english river.the successful colonization, establishment and spread of pomphorhynchus laevis in a small, isolated, devon river 128 km from the parasite's nearest focus in dorset was followed over 11 years from 1985. the parasite was first detected in anguilla anguilla and platichthys flesus in 1988: by 1995 it had attained prevalence levels of 22.6% in a. anguilla and 43.6% in p. flesus and also occurred in 100% salmo trutta, 50% cottus gobio and noemacheilus barbatulus. as judged by prevalence, abundance, pr ...19968991914
functional comparison of the metal-regulated transcriptional control regions of metallothionein genes from cadmium-sensitive and tolerant fish species.the promoter region of teleost metallothioneins (mts) contains multiple metal-responsive elements (mres) organized in proximal and distal clusters which together mediate gene induction by heavy metals. this arrangement of mres is found both in cadmium-sensitive species, such as the rainbow trout, and in cadmium-tolerant species such as the pike and the stone loach. on comparison of the putative regulatory elements identified within the 5'-flanking region of these genes the major differences are ...19979061029
long-term recovery of macrobenthos and fish assemblages after water pollution abatement measures in the river petite baïse (france).riverine ecosystems are subject to a large variety of man-made influences, and in recent years a new public awareness of the need to protect rivers has emerged within the industrialized world. the present study focuses on the effect of abatement of pollution from one factory on the recovery of fish and macroinvertebrate species richness in the river petite baïse over a 20-year period (1973-1993). until 1973, the petite baïse (75 km in length) received not only factory waste (nitrogenous rich eff ...19979353205
impact assessment of a wastewater treatment plant effluent using the fish biomarker ethoxyresorufin-o-deethylase: field and on-site experiments.the impact of a wastewater treatment plant (wwtp) effluent was assessed with the fish biomarker ethoxyresorufin-o-deethylase (erod) using field and on-site laboratory experiments. erod activity was measured in chub (leuciscus cephalus) and stone loach (noemacheilus barbatulus) caught at three sites of the chalaronne river (southeast france). liver somatic index (lsi) and organochloride bioaccumulation in muscle were estimated for chub only. in september, erod activity and lsi of chub increased s ...19989756685
ultrastructural changes in fish gills as biomarker to assess small stream pollution.in order to verify the principal suitability of gill ultrastructure as a biomarker, semi-field studies with two endigoneous fish, trout (salmo trutta f. fario) and loach (barbatula barbatula), were performed. the fish were exposed in flow-through systems to one heavily polluted (körsch) and one lightly polluted small stream (krähenbach) in south-west germany. ultrastructural responses in gills were correlated with limnological and chemical data recorded over a 2 year period in each stream. after ...199810189321
observations on the metazoan parasites of the atlantic salmon (salmo salar) after its reintroduction into the elbe river basin in the czech republic.in the years 1999-2002, first studies were carried out on the metazoan parasites of atlantic salmon (salmo salar l.) recently reintroduced into the elbe river drainage system, after more than 50 years after the complete extinction of the elbe salmon population. a total of six helminth species were recorded from salmon smolts from three streams of the elbe river basin (kamenice river, jestedský and libocanský brooks) in north bohemia, czech republic, where s. salar fingerlings have been released ...200314971599
[helminths fauna of fishes in the mała panew river drainage basin].a parasitological investigation was made at 420 fishes from 10 species: perca fluviatilis l., gymnocephalus cernuus (l.), gobio gobio (l.), tinca tinca (l.), leuciscus idus (l), noemacheilus barbatulus (l.), lota lota (l.), leucaspisus delineatus (heckell 843), blicca biorkna (l.) and rhodeus sericeus amarus (bloch 1782). the fishes were caught over one year period (1995-1996) in the mała panew river drainage basin (south poland). in examined fishes the following parasite species were found: dip ...200016886354
experimental studies on the development of contracaecum rudolphii (nematoda: anisakidae) in copepod and fish paratenic hosts.the larval development of the nematode contracaecum rudolphii (rudolphi, 1819), a common parasite of the proventriculus of cormorants, was experimentally studied. within the eggs cultivated in freshwater under laboratory temperatures of 20-22 degrees c, the developing larva undergoes two moults on days 4-5, attaining the third larval stage. most of the ensheathed third-stage larvae, 291-457 microm long, hatch spontaneously from egg shells on days 5-6. experiments have indicated that hatched ensh ...200919827362
chemical contaminants in fish species from rivers in the north of luxembourg: potential impact on the eurasian otter (lutra lutra).contamination levels of pcbs, and of the heavy metals cadmium (cd), lead (pb) and mercury (hg) were analyzed in four fish species from seven rivers in the north of luxembourg. during august and september 2007, 85 samples of fish were collected belonging to four species: the stone loach (barbatula barbatula, n=12 pools), the chub (squalius cephalus, n=36), the barbel (barbus barbus, n=23) and eel (anguilla anguilla, n=14). the concentration of seven indicator pcbs ( summation operator(7)pcbs) rea ...201020060148
gyrodactylus laevisoides n. sp. (monogenea: gyrodactylidae) infecting northern redbelly dace phoxinus eos cope (cyprinidae) from nova scotia, canada.gyrodactylus laevisoides n. sp. is described from the gill rakers of red belly dace, phoxinus eos cope (cyprinidae), from nova scotia, canada. gyrodactylus laevisoides n. sp. is the second species of gyrodactylus nordmann, 1832 described from this host and is characterised by weakly curving hamuli, a small ventral bar lacking anterolateral processes, stout dorsal bar, small marginal hooks with sickles larger proximally than distally and having a small circular process on the heel, a mco with spi ...201324163028
habitat use by the fish assemblages of two chalk streamspatterns of habitat use by fish assemblages in two chalk streams in southern england were examined to identify species preferences with respect to major habitat gradients. both study sites, although differing in some physical habitat characteristics, mainly channel width, water temperature and instream cover, could be arranged similarly along a continuum extending from erosional to depositional habitats. twelve fish species were collected from stream surveys conducted during july 1993. the habit ...19979236089
proteocephalid tapeworms (cestoda: onchoproteocephalidea) of loaches (cobitoidea): evidence for monophyly and high endemism of parasites in the far east.the parasite fauna of loaches (cypriniformes: cobitoidea), a group of small bottom-dwelling freshwater fishes with a mostly eurasian distribution, remains a largely unknown quantity. here we revise the taxonomy of tapeworms of the genus proteocephalus weinland, 1858 (cestoda: proteocephalidae) that had been found in loaches from the palaearctic region (central europe, japan and russia [primorsky region]). molecular phylogenetic analysis based on two nuclear (ssr- and lsrdna) and two mitochondria ...201727702602
molecular faunistics of accidental infections of gyrodactylus nordmann, 1832 (monogenea) parasitic on salmon salmo salar l. and brown trout salmo trutta l. in nw russia.salmon salmo salar l. and brown trout s. trutta l. juveniles were examined for the presence of accidental monogenean ectoparasitic species of gyrodactylus nordmann, 1832 in the baltic and white sea basins of russian karelia in order to estimate the frequency of host-switching attempts on an ecological timescale. to collect phylogeographical information and for exact species identification, the parasites were characterised by nuclear internal transcribed spacer sequences of rdna (its) and, for so ...200818038199
morphometric comparison of three members of the gyrodactylus nemachili-like species group (monogenea: gyrodactylidae) on barbatula barbatula l. in the czech republic, with a reinstatement of g. papernai ergens & bychowsky, 1967.the gyrodactylus nemachili-like species complex, parasitising stone loach barbatula barbatula l. in the czech republic, was investigated based on the detailed morphometric analysis of its haptoral hard parts. the existence of two species, g. jiroveci ergens & bychowsky, 1967 and g. pseudonemachili ergens & bychowsky, 1967 was confirmed. a standard discriminant analysis of the morphometric data showed a clear separation between specimens of g. jiroveci and another species or variant. based on the ...200818030600
cytochrome p4501a induction in brown trout exposed to small streams of an urbanised area: results of a five-year-study.this case study examines the ability of the cytochrome p4501a (cyp1a) biomarker to distinguish the pollution status of two small streams, krähenbach and körsch, receiving different levels of urban and agricultural impact, with low to moderate contamination by arylhydrocarbon receptor (ahr)-binding pahs and pcbs. brown trout, salmo trutta, exposed in enclosure restrictions, showed significant between-stream differences of hepatic cyp1a levels. erod activities were the better discriminator than cy ...200515840531
cloning of a chub metallothionein cdna and development of competitive rt-pcr of chub metallothionein mrna as a potential biomarker of heavy metal exposure.metallothionein has been assayed in a range of aquatic animal tissues as an indicator of metal exposure. we sequenced chub (leuciscus cephalus) metallothionein cdna which showed over 90% homology to common carp, goldfish and stone loach and 77% homology to rainbow trout sequences for metallothionein. we then used the extended primer method to develop an accurate quantitative competitive rt-pcr assay for metallothionein mrna. rt-pcr was used to measure metallothionein mrna in feral chub from a ra ...201515178096
old fish in a young lake: stone loach (pisces: barbatula barbatula) populations in lake constance are genetically isolated by distance.the genetic structure of 10 populations (453 individuals) of stone loach (barbatula barbatula l.), a small bottom-dwelling cyprinid fish, in the littoral zone of lake constance, central europe, was investigated by analysing the mitochondrial control region sequences and five microsatellite loci. an unexpectedly high degree of genetic diversity (up to 0.36%) and old estimated age of these populations (> 150 000 years) based on mitochondrial dna (mtdna) was found. these findings contrast with the ...200515773949
associations between fish reproductive cycle and the dynamics of metazoan parasite infection.the parasite fauna of the stone loach (barbatula barbatula) was investigated monthly from may to december 2001. four parasite species were recorded: gyrodactylus spp. (monogenea), tylodelphys clavata (digenea), proteocephalus sagittus (cestoda), and raphidascaris acus (nematoda). the changes in the seasonal dynamics of parasite infection were investigated throughout the 8 months. the potential effect of the reproductive investment of the host, measured by gonad mass and gonado-somatic index (gsi ...200515565460
loaches in a long term study of the drava river in croatia.the diversity, species composition and structure of the fish community were studied in the vicinity of three power plant reservoirs in the middle part of the drava river in croatia. over a period of twenty years in a collection of 25,913 fishes, 56 different fish species belonging to 16 families were recorded. the family cobitidae is represented by two species: cobitis elongatoides and misgurnus fossilis, while the family balitoridae is represented by only one species, barbatula barbatula. the p ...200315303354
raphidascaris acus (bloch, 1779) larvae infections of the stone loach, barbatula barbatula (l.), from the river haná, czech republic.the stone loach, barbatula barbatula (l.), is a typical and dominant intermediate host of raphidascaris acus in lowland streams of central europe. the prevalence of infection of r. acus in b. barbatula from the river haná ranged throughout the year from 73.3 to 100%. the abundance and the mean intensity of infection also varied throughout the year with a peak in september. larvae were located mainly in the liver parenchyma. high numbers of larvae and their migration through the tissue caused cys ...200415009247
a mathematical model for cadmium in the stone loach (noemacheilus barbatulus l.) from the river ecclesbourne, derbyshire.a mathematical model which linked metabolism of stone loach with cadmium dynamics was developed using information from laboratory experiments and field studies. three possible sources of cadmium were distinguished: water, food, and sediment. predicted results over 1 year were compared with field observations from three sites in derbyshire. the model adequately predicted growth of fish for all three sites. predicted cadmium levels in loach were in good agreement with measured levels in fish from ...19902338063
development of proteocephalus torulosus in the intermediate host under experimental conditions.the development of the tapeworm proteocephalus torulosus (batsch, 1786) (cestoda: proteocephalidae), a parasite of cyprinid fish, was studied in the intermediate host under experimental conditions. the eggs of p. torulosus were typified by a relatively small outer envelope (hyaline membrane) and a thick middle layer surrounding the oncosphere. incubation of p. torulosus eggs at different temperatures revealed the ability of some oncospheres to survive and remain infective to the intermediate hos ...19938132977
do sewage treatment plant discharges substantially impair fish reproduction in polluted rivers?sewage treatment plants are frequently associated with the release of xenobiotics and, consequently, with alterations of the reproductive function induced by many of these substances in aquatic organisms. in order to assess the impacts of sewage treatment plant (stp) discharges in polluted rivers, two sentinel species (gudgeon gobio gobio and stoneloach barbatula barbatula) were caught during their reproductive cycle upstream and downstream two stps (stp1--goffontaine, stp2--wegnez). gonadosomat ...200717112566
comparison of ultrastructural and morphometrical analysis of tonic, white and red muscle fibers in the myotome of teleost fish (noemacheilus barbatulus l.). 19873433896
around or across the carpathians: colonization model of the danube basin inferred from genetic diversification of stone loach (barbatula barbatula) populations.despite increasing information about postglacial recolonization of european freshwater systems, very little is known about pre-pleistocene history. we used data on the recent distribution and phylogenetic relationships of stone loach mitochondrial lineages to reconstruct the initial colonization pattern of the danube river system, one of the most important refuges for european freshwater ichthyofauna. fine-scale phylogeography of the danubian populations revealed five highly divergent lineages o ...200818302688
life-history traits of the stone loach barbatula barbatula.the life-history tactics of the stone loach barbatula barbatula were studied in a mediterranean-type climate stream (matarranya river) located in the ebro river basin (north-east spain). maximum observed ages were 2+ years in both sexes (1% of individuals), although only 0+ and 1+ year age groups were well represented. it is the lowest longevity reported for this species in its entire distribution. the seasonal growth period started in june and continued until november, but the pattern observed ...201020646136
a comparison of the differential accumulation of cadmium in the tissues of three species of freshwater fish, salmo gairdneri, rutilus rutilus and noemacheilus barbatulus.roach and stone loach were exposed to cadmium dissolved in their aquarium water at 500 and 1250 micrograms/l, respectively, and the distribution of the metal accumulated in the major body organs was determined. the pattern of distribution for each species was somewhat different and was distinct in each case from that observed previously with rainbow trout. the total body loads of cadmium accumulated by the three species were assessed during the period of exposure and found not to correlate direc ...19862874940
complete mitochondrial genome of the stone loach, triplophysa stoliczkae (teleostei: cypriniformes: balitoridae).the complete mitochondrial genome of the stone loach triplophysa stoliczkae is 16,571 bp in size, consisting of 13 protein-coding genes, 22 transfer rna (trna) genes, two ribosomal rna (rrna) genes, and a noncoding control region. as in other vertebrates, most mitochondrial genes are encoded on the heavy strand, except for nd6 and eight trna genes which are encoded on the light strand. the overall base composition of the heavy strand of the t. stoliczkae mitochondrial genome is a: 28.1%, t: 29.0 ...201322920274
estimation of parameters in a bertalanffy type of temperature dependent growth model using data on juvenile stone loach (barbatula barbatula).parameters of a bertalanffy type of temperature dependent growth model are fitted using data from a population of stone loach (barbatula barbatula). over two periods respectively in 1990 and 2010 length data of this population has been collected at a lowland stream in the central part of the netherlands. the estimation of the maximum length of a fully grown individual is given special attention because it is in fact found as the result of an extrapolation over a large interval of the entire life ...201223053466
[electron microscopical and autoradiographical studies on the so-called yolk-nuclei in the oocytes of nemacheilus barbatulus (l.) and phoxinus phoxinus (l.) (pisces, teleostei) (author's transl)].the yolk-nuclei in the oocytes of the teleosts noemacheilus barbatulus (l.) and phoxinus phoxinus (l.) were investigated by electron microscopy, autoradiography and ultrahistochemistry. yolk-nuclei were only found in stage i. they consist of mitochondria, an osmiophilic material and in noemacheilus partly of dictyosomes. it was demonstrated by marking with [3h]-uridine, that the osmiophilic material contains rna. the osmiophilic material gets into the juxtanuclear cytoplasm. there it is surround ...1978689247
[the yolk nucleus in young oocytes of neomacheilus barbatulus (l.) (teleostei, cobitidae) (author's tranls)].the yolk nucleus in the young oocytes of the freshwater teleost noemacheilus barbatulus (l.) was investigated by electron microscopy. yolk nuclei are only found in stage i. they consist of nucleolar substance, mitochondria and partly of dictysomes. the nucleolar material leaves the nucleus through the pores of the nuclear membrane and gets into the juxtanuclear cytoplasm. it mainly consists of ribonucleic acid. the yolk nuclei of noemacheilus doesn't have any relations to the formation of yolk.2016138302
the complete mitochondrial genome of beautiful stone loach traccatichthys pulcher (nichols & pope) (cypriniformes: balitoridae).in this study, the complete mitogenome sequence of balitorid fish, beautiful stone loach traccatichthys pulcher (nichols & pope, 1927), collected from hainan island, china, has been sequenced by the long polymerase chain reaction method. the mitogenome, consisting of 16,580 base pairs (bp), had typical vertebrate mitochondrial gene arrangement, including 13 protein coding genes, 22 transfer rnas, 2 ribosomal rna genes and a noncoding control region (cr). cr of 928 bp length is located between tr ...201524409869
schistura phamhringi, a new stone loach from chindwin basin in manipur, india (cypriniformes: nemacheilidae).schistura phamhringi, new stone loach, is described from dutah stream, tributary of the yu river (chindwin basin), near larong village, chandel district, manipur, india. it is distinguished from all its congeners by a unique combination of characters: 6-7 black saddles, each continued on both flanks forming broad diamond-shaped black bars with narrow ventral margin; bars superimposed on a grey stripe along lateral line; upper lip with numerous melanophores; black basicaudal bar arc-shaped; compl ...201424869534
the complete mitochondrial genome of giant stone loach triplophysa siluroides (cypriniformes: balitoridae).in this study, the complete mitogenome sequence of balitorid fish, giant stone loach triplophysa siluroides, which collected from the yellow river, china has been sequenced by the long polymerase chain reaction method. the mitogenome, consisting of 16,574 base pairs (bp), had typical vertebrate mitochondrial gene arrangement, including 13 protein coding genes, 22 transfer rnas, 2 ribosomal rna genes and a noncoding control region (cr). cr of 918 bp length is located between trna(pro) and trna(ph ...201624963763
molecular diversity of germany's freshwater fishes and lampreys assessed by dna barcoding.this study represents the first comprehensive molecular assessment of freshwater fishes and lampreys from germany. we analysed coi sequences for almost 80% of the species mentioned in the current german red list. in total, 1056 dna barcodes belonging to 92 species from all major drainages were used to (i) build a reliable dna barcode reference library, (ii) test for phylogeographic patterns, (iii) check for the presence of barcode gaps between species and (iv) evaluate the performance of the bar ...201525186809
complete mitochondrial genome of triplophysa bleekeri (cypriniformes: balitoridae: nemacheilinae), and analysis of mitochondrial genetic variability among triplophysa species.the complete mitochondrial genome of the stone loach triplophysa bleekeri collected from the anning river, a tributary of the yalong river, in sichuan province was sequenced. the genome is 16,573 bp in length, consisting of 13 protein-coding genes, 22 transfer rna (trna) genes, two ribosomal rna (rrna) genes, and a noncoding control region. most of the genes are encoded on the heavy strand, except for nd6 and eight trnas. the overall base composition of t. bleekeri is 28.1% for a, 28.4% for t, 2 ...201625600735
pcr-free shotgun sequencing of the stone loach mitochondrial genome (barbatula barbatula).the complete mitochondrial genome of the stone loach barbatula barbatula (linnaeus, 1758) (actinopterygii: cypriniformes: nemacheilidae) has been sequenced using a genome-skimming approach on an illumina hiseq 2500 platform. the mitochondrial genome of b. barbatula was determined to be 16,630 bp long and presents an organization typical of vertebrate mitogenomes. the mean coverage was 82× with a minimum coverage of 33× for the control region and 52× for the remaining part of the genome. a phylog ...201626000945
sequence and organization of the complete mitogenome of a siberian stone loach, barbatula toni (dybowsky, 1869) (cypriniformes: balitoridae).in this study, we determined the complete mitogenome sequence of siberian stone loach, barbatula toni (dybowsky, 1869). the total length of mitogenome is 16 623 bp, which consists of 13 protein-coding genes, 22 trna genes, 2 rrna genes, and 1 control region (d-loop). the genome organization and gene order were identical to that of the typical vertebrates. the control region harbors conserved sequence blocks (csb-d, e, f, csb-1, cbs-2 and cbs-3) and ta-nucleotide microsatellite repeats in its 3' ...201626305807
karyotype differentiation in 19 species of river loach fishes (nemacheilidae, teleostei): extensive variability associated with rdna and heterochromatin distribution and its phylogenetic and ecological interpretation.loaches of the family nemacheilidae are one of the most speciose elements of palearctic freshwater ichthyofauna and have undergone rapid ecological adaptations and colonizations. their cytotaxonomy is largely unexplored; with the impact of cytogenetical changes on this evolutionary diversification still unknown. an extensive cytogenetical survey was performed in 19 nemacheilid species using both conventional (giemsa staining, c- banding, ag- and chromomycin a3/dapi stainings) and molecular (fluo ...201526573692
balitora chipkali, a new species of stone loach (teleostei: balitoridae) from the northern western ghats of india, with a note on the distribution of b. laticauda.balitora chipkali, a new species of stone loach, is described from the westward-flowing astoli tributary of the kali river system in the northern part of the western ghats, india. the species differs from its congeners in a combination of characters that includes: a single pair of maxillary barbels; a large eye, of diameter greater than 15% head length (hl); snout length less than 4 times eye diameter; gape of mouth less than 30% hl; upper lip with 9-12 papillae in first series and 3-8 papillae ...201627470757
the first european cave fish.subterranean biodiversity in europe is spectacularly rich, with the western balkans being home to about 400 cave species, representing the highest number of species per area worldwide [1]. nonetheless, cave fishes, which are the most commonly found vertebrates in underground habitats [2], have not been described from europe so far [3]. here, we report the first european record of a cave fish population, a loach of the genus barbatula (figure 1), found in the danube-aach system, an underground ka ...201728376329
co-occurrence patterns of some small-bodied freshwater fishes in southwestern france: implications for fish conservation and environmental management.we assessed the influence of environmental variables (elevation, stream order, distance from source, catchment area, slope, stream width, and fish species richness) on the co-occurrence patterns of the minnow, the stone loach, and the gudgeon at the stream system scale. a total of 474 sites were classified according to the seven variables using the self-organizing map (neural network), and three clusters were detected (k-means algorithm). the frequency of the various fish co-occurrence patterns ...200516201214
the complementarity of chemosensory systems in mediating the searching behavioral response to food chemical signals in stone loach barbatula barbatula. 201616121944
seasonal dynamics of proteocephalus sagittus in the stone loach barbatula barbatula from the hana river, czech republic.the seasonal cycle of the cestode proteocephalus sagittus (cestoda: proteocephalidae) was studied for the first time in the stone loach barbatula barbatula from the hana river, czech republic. a total of 180 loaches were examined monthly from january to december 2001. the parasite occurred in loaches throughout the year but infection parameters differed significantly among seasons, with the highest values of prevalence and abundance from the late winter to the early summer. parasite recruitment ...200415469625
elucidation of cdna sequences for metallothioneins from rainbow trout, stone loach and pike liver using the polymerase chain reaction.metallothionein cdnas were generated from the livers of three fish species and amplified by pcr. two distinct coding sequences (a and b) were elucidated for rainbow trout metallothioneins but single isoforms were encoded by genes isolated from the stone loach and pike. different sized transcripts were observed both with stone loach and pike but these were accounted for by altered lengths of 3' untranslated regions.20061859844
reproduction of the stone loach, barbatula barbatula (l.) in estonia.reproduction of the stone loach was investigated in two rivers of northwestern estonia (vääna and maidla, 59 degrees n). loaches in the vääna river were slow growing and males prevailed among older fish; in the maidla river loaches were fast growing and females dominated among older fish. reproduction indices (age at maturation, duration of the spawning season, number of egg batches, fecundity, oocyte diameter, annual dynamics of gonadosomatic index and oocyte diameter) of comparably sized loach ...200315303363
resurrection of proteocephalus sagittus (grimm, 1872) (cestoda: proteocephalidea) based on morphological and molecular data.this paper presents a redescription of the proteocephalidean cestode proteocephalus sagittus (grimm, 1872) based on freshly collected specimens from the type-host, the stone loach barbatula barbatula (l.) (family balitoridae), from the czech republic. this species has recently been synonymised with p. torulosus (batsch, 1786), a parasite of cyprinid fishes in the holarctic region, but it differs in the following characters: (i) scolex morphology (scolex rounded, with more apically situated sucke ...200314707502
fish community responses to pulp and paper mill effluents at the southern lake saimaa, finland.the fish community in sublittoral and profundal waters, at stony shores, and densities of vendace larvae were studied in the southern lake saimaa, finland. the objective was to investigate the possible recovery of fish populations after modernizations at the pulp and paper mills discharging into the lake. sublittoral and profundal waters were studied by gill net trial fishings, stony shores by electrofishing, and vendace larvae by beach seine. the research area was divided in a polluted (0.5-4.0 ...200211806460
analysis of regulatory elements flanking metallothionein genes in cd-tolerant fish (pike and stone loach).from genomic libraries constructed for both pike and stone loach, clones were isolated containing the metallothionein genes from these two species of cadmium-tolerant fish. a single copy metallothionein gene was identified in each species by southern blot analysis. sequencing revealed that each gene consisted of three exons followed by polyadenylation signals at the 3' end. in the 5' flanking region, putative metal responsive elements were identified both close to the transcription start site an ...19938218416
[injuries to chemical sense organs of fish due to sublethal levels of water pollution].histopathological investigations of taste organs of loaches (noemacheilus barbatulus, l.) taken from creeks with differing grades of pollution demonstrate variable degrees of damage to taste bud structures. the degree of cell and tissue alteration (including sensory cells) is obviously directly correlated to the level and effective duration of the type of water pollutant. based on the appearance of taste bud damage due to sublethal grades and qualities of pollution, the gustatory organs of fish ...19912068720
metallothionein gene expression and cadmium toxicity in freshwater fish.certain species of fish e.g. rainbow trout, are particularly susceptible to poisoning by cadmium in their aquatic environment whereas others e.g. roach and stone loach, are much more resistant. it is postulated that the vulnerability of the salmonids arises because 1) existing metallothionein (mt) in the tissues of these fish is unable to bind cadmium and 2) the toxic metal (in contrast to zinc) cannot switch on the gene(s) for apo-thionein production de novo. consequently, cadmium is sequestere ...19872959554
sequestration of environmental cadmium by metallothionein in the roach (rutilus rutilus) and the stone loach (noemacheilus barbatulus).two species of coarse fish that are relatively resistant to cadmium poisoning were exposed to sub-lethal concentrations of the metal in their aquarium water. thus, roach were exposed to cadmium concentrations between 30 and 500 micrograms/l for periods of 14-70 days whereas stone loach were exposed to 1250 micrograms cd/l for 21-77 days. under all conditions of exposure, it was found upon analysis of the major organs of accumulation of cadmium in the two species that the toxic metal was sequeste ...19872885141
histochemical and electronmicroscopical analysis of muscle fiber in myotomes of teleost fish (noemacheilus barbatulus l.).a quantitative histochemical study was carried out on axial musculature of noemacheilus barbatulus l. on the basis of succinate dehydrogenase (sdh) and myofibrillar atp-ase activity, 5 types of muscle fibers are described. when the sdh method was used, red, tonic, intermediate, and white muscle fibers were easily observed. however, histochemical reaction for myofibrillar atp-ase activity, after alkaline preincubation (ph = 10.4), revealed another type of fiber zone laying between the intermediat ...19853158569
the production of cadmium-binding protein in three species of freshwater fish.cadmium-binding protein (cd-bp) was detected in the liver and kidney of stoneloach, noemacheilus barbatulus, roach, rutilus rutilus and rainbow trout, salmo gairdneri which had been dosed i.p. with 1 mg cadmium (cd)/kg body weight 168 h previously. the cadmium content (microgram cd/g wet weight tissue) was much lower in the liver and kidney of trout than in stoneloach and roach, but there was no clear relationship between the amount of cd-bp detected and the reported sensitivity of each species ...19836836595
lead and cadmium in stone loach (noemacheilus barbatulus l.) from three rivers in derbyshire.fish noemacheilus barbatulus l. (stone loach) were caught at about 4-week intervals from single sites in three derbyshire rivers, with different concentrations of cadmium and lead in sediments and water, during a 1-year sampling program. fish were classified by age, site, and sampling occasion. growth was allometric and affected by temperature. a steady state of cadmium burden was reached by fish of 2 years old or more but not by younger fish. for lead, fish rarely showed an increase in body bur ...19892776688
metabolic rate and uptake and loss of cadmium from food by the fish noemacheilus barbatulus l. (stone loach).fish, noemacheilus barbatulus (stone loach), of different body weights were used to study rates of uptake and loss of cadmium during and after dietary exposure. fish were kept singly in a continuous-flow system, and fed tubificid worms. the worms had a range of cadmium levels, but all levels were below that needed to cause acute lethal toxicity in the fish. body weight affected both the maintenance ration and the amount of food consumed ad libitum, but the exponent for body weight (0.78+/-0.04), ...198915092402
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